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- PDB-8zsb: Cryo-EM structure of human ZnT1, in the absence of zinc, determin... -
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Basic information
Entry | Database: PDB / ID: 8zsb | ||||||
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Title | Cryo-EM structure of human ZnT1, in the absence of zinc, determined in outward-facing conformation | ||||||
![]() | Proton-coupled zinc antiporter SLC30A1 | ||||||
![]() | TRANSPORT PROTEIN / Copper uptake / Cuproptosis / ZnT family | ||||||
Function / homology | ![]() negative regulation of zinc ion transmembrane import / zinc export across plasma membrane / detoxification of zinc ion / detoxification of cadmium ion / Zinc efflux and compartmentalization by the SLC30 family / cadmium ion transmembrane transport / zinc ion import into organelle / zinc:proton antiporter activity / zinc ion transmembrane transporter activity / zinc ion transport ...negative regulation of zinc ion transmembrane import / zinc export across plasma membrane / detoxification of zinc ion / detoxification of cadmium ion / Zinc efflux and compartmentalization by the SLC30 family / cadmium ion transmembrane transport / zinc ion import into organelle / zinc:proton antiporter activity / zinc ion transmembrane transporter activity / zinc ion transport / zinc ion transmembrane transport / negative regulation of calcium ion import / negative regulation of neurotransmitter secretion / intracellular zinc ion homeostasis / calcium ion import / calcium channel inhibitor activity / T-tubule / postsynaptic density membrane / cytoplasmic vesicle membrane / Schaffer collateral - CA1 synapse / intracellular calcium ion homeostasis / basolateral plasma membrane / nuclear membrane / in utero embryonic development / defense response to bacterium / Golgi apparatus / endoplasmic reticulum / metal ion binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.26 Å | ||||||
![]() | Ma, J. / Zheng, S. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Zinc transporter 1 functions in copper uptake and cuproptosis Authors: Li, Y. / Ma, J. / Wang, R. / Luo, Y. / Zheng, S. / Wang, X. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 121 KB | Display | ![]() |
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PDB format | ![]() | 91.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 60410MC ![]() 8zszC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein | Mass: 55362.605 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Chemical | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Cryo-EM structure of the ZnT1 in the apo state / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() |
Buffer solution | pH: 7.4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal magnification: 64000 X / Nominal defocus max: 3250 nm / Nominal defocus min: 1230 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Num. of real images: 890 |
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Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 3.26 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 709780 / Symmetry type: POINT | ||||||||||||||||||||||||
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