+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 8xou | |||||||||
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タイトル | Prohead portal vertex of bacteriophage lambda | |||||||||
要素 |
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キーワード | VIRAL PROTEIN / caudovirales / siphoviridae / portal vertex / portal / capsid / connector/neck / tail / delivery device / B-DNA / phage lambda | |||||||||
機能・相同性 | 機能・相同性情報 viral portal complex / symbiont genome ejection through host cell envelope, long flexible tail mechanism / T=7 icosahedral viral capsid / virion assembly / viral capsid / host cell cytoplasm / structural molecule activity / DNA binding 類似検索 - 分子機能 | |||||||||
生物種 | Escherichia phage Lambda (λファージ) | |||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 5.58 Å | |||||||||
データ登録者 | Wang, J.W. / Gu, Z.W. | |||||||||
資金援助 | 中国, 2件
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引用 | ジャーナル: J Virol / 年: 2024 タイトル: Structural morphing in the viral portal vertex of bacteriophage lambda. 著者: Zhiwei Gu / Kexun Wu / Jiawei Wang / 要旨: The portal protein of tailed bacteriophage plays essential roles in various aspects of capsid assembly, motor assembly, genome packaging, connector formation, and infection processes. After DNA ...The portal protein of tailed bacteriophage plays essential roles in various aspects of capsid assembly, motor assembly, genome packaging, connector formation, and infection processes. After DNA packaging is complete, additional proteins are assembled onto the portal to form the connector complex, which is crucial as it bridges the mature head and tail. In this study, we report high-resolution cryo-electron microscopy (cryo-EM) structures of the portal vertex from bacteriophage lambda in both its prohead and mature virion states. Comparison of these structures shows that during head maturation, in addition to capsid expansion, the portal protein undergoes conformational changes to establish interactions with the connector proteins. Additionally, the independently assembled tail undergoes morphological alterations at its proximal end, facilitating its connection to the head-tail joining protein and resulting in the formation of a stable portal-connector-tail complex. The B-DNA molecule spirally glides through the tube, interacting with the nozzle blade region of the middle-ring connector protein. These insights elucidate a mechanism for portal maturation and DNA translocation within the phage lambda system. IMPORTANCE: The tailed bacteriophages possess a distinct portal vertex that consists of a ring of 12 portal proteins associated with a 5-fold capsid shell. This portal protein is crucial in multiple ...IMPORTANCE: The tailed bacteriophages possess a distinct portal vertex that consists of a ring of 12 portal proteins associated with a 5-fold capsid shell. This portal protein is crucial in multiple stages of virus assembly and infection. Our research focused on examining the structures of the portal vertex in both its preliminary prohead state and the fully mature virion state of bacteriophage lambda. By analyzing these structures, we were able to understand how the portal protein undergoes conformational changes during maturation, the mechanism by which it prevents DNA from escaping, and the process of DNA spirally gliding. | |||||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 8xou.cif.gz | 3 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb8xou.ent.gz | 表示 | PDB形式 | |
PDBx/mmJSON形式 | 8xou.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 8xou_validation.pdf.gz | 1.7 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 8xou_full_validation.pdf.gz | 1.7 MB | 表示 | |
XML形式データ | 8xou_validation.xml.gz | 356 KB | 表示 | |
CIF形式データ | 8xou_validation.cif.gz | 499.9 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/xo/8xou ftp://data.pdbj.org/pub/pdb/validation_reports/xo/8xou | HTTPS FTP |
-関連構造データ
関連構造データ | 38541MC 8xotC 8xowC 8xpmC 8xqbC C: 同じ文献を引用 (文献) M: このデータのモデリングに利用したマップデータ |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
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非結晶学的対称性 (NCS) | NCSドメイン:
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