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Open data
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Basic information
Entry | Database: PDB / ID: 8x91 | ||||||
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Title | P/Q type calcium channel in complex with omega-conotoxin MVIIC | ||||||
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![]() | MEMBRANE PROTEIN / voltage-gated calcium channel in complex with toxins | ||||||
Function / homology | ![]() regulation of membrane repolarization during action potential / Presynaptic depolarization and calcium channel opening / positive regulation of high voltage-gated calcium channel activity / calcium ion transmembrane transport via high voltage-gated calcium channel / membrane depolarization during bundle of His cell action potential / high voltage-gated calcium channel activity / L-type voltage-gated calcium channel complex / host cell presynaptic membrane / cardiac muscle cell action potential involved in contraction / NCAM1 interactions ...regulation of membrane repolarization during action potential / Presynaptic depolarization and calcium channel opening / positive regulation of high voltage-gated calcium channel activity / calcium ion transmembrane transport via high voltage-gated calcium channel / membrane depolarization during bundle of His cell action potential / high voltage-gated calcium channel activity / L-type voltage-gated calcium channel complex / host cell presynaptic membrane / cardiac muscle cell action potential involved in contraction / NCAM1 interactions / regulation of ventricular cardiac muscle cell membrane repolarization / calcium ion transport into cytosol / regulation of calcium ion transmembrane transport via high voltage-gated calcium channel / syntaxin binding / voltage-gated calcium channel complex / ion channel inhibitor activity / neuromuscular junction development / neuronal dense core vesicle / regulation of heart rate by cardiac conduction / regulation of calcium ion transport / response to amyloid-beta / calcium channel regulator activity / calcium ion import across plasma membrane / voltage-gated calcium channel activity / sarcoplasmic reticulum / cell projection / Regulation of insulin secretion / protein localization to plasma membrane / calcium ion transmembrane transport / modulation of chemical synaptic transmission / Adrenaline,noradrenaline inhibits insulin secretion / cellular response to amyloid-beta / calcium ion transport / amyloid-beta binding / T cell receptor signaling pathway / toxin activity / positive regulation of cytosolic calcium ion concentration / chemical synaptic transmission / neuronal cell body / synapse / extracellular exosome / extracellular region / membrane / nucleus / metal ion binding / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.11 Å | ||||||
![]() | Yan, N. / Li, Z. / Cong, Y. / Wu, T. / Wang, T. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis for different ω-agatoxin IVA sensitivities of the P-type and Q-type Ca2.1 channels. Authors: Zhangqiang Li / Ye Cong / Tong Wu / Tongtong Wang / Xinyao Lou / Xinyu Yang / Nieng Yan / ![]() | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 539.5 KB | Display | ![]() |
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PDB format | ![]() | 414.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.1 MB | Display | ![]() |
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Full document | ![]() | 2.2 MB | Display | |
Data in XML | ![]() | 83.8 KB | Display | |
Data in CIF | ![]() | 124.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 38159MC ![]() 8x90C ![]() 8x93C M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
-Voltage-dependent ... , 3 types, 3 molecules CBA
#1: Protein | Mass: 56231.570 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#3: Protein | Mass: 126316.148 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#4: Protein | Mass: 287534.312 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
-Protein/peptide , 1 types, 1 molecules X
#2: Protein/peptide | Mass: 2763.341 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() |
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-Sugars , 4 types, 7 molecules ![](data/chem/img/NAG.gif)
#5: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source | ||||
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#6: Polysaccharide | Source method: isolated from a genetically manipulated source #7: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #8: Sugar | |
-Non-polymers , 5 types, 14 molecules ![](data/chem/img/CA.gif)
![](data/chem/img/CLR.gif)
![](data/chem/img/PT5.gif)
![](data/chem/img/Y01.gif)
![](data/chem/img/3PE.gif)
![](data/chem/img/CLR.gif)
![](data/chem/img/PT5.gif)
![](data/chem/img/Y01.gif)
![](data/chem/img/3PE.gif)
#9: Chemical | #10: Chemical | ChemComp-CLR / #11: Chemical | ChemComp-PT5 / [( | #12: Chemical | #13: Chemical | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Voltage-gated P/Q type calcium channel / Type: COMPLEX / Entity ID: #1-#4 / Source: RECOMBINANT |
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Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 1800 nm / Nominal defocus min: 1300 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
EM software | Name: PHENIX / Version: 1.19.2_4158: / Category: model refinement | ||||||||||||||||||||||||
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.11 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 126389 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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