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- EMDB-38159: P/Q type calcium channel in complex with omega-conotoxin MVIIC -

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ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-38159
TitleP/Q type calcium channel in complex with omega-conotoxin MVIIC
Map data
Sample
  • Complex: Voltage-gated P/Q type calcium channel
    • Protein or peptide: Voltage-dependent L-type calcium channel subunit beta-3
    • Protein or peptide: Omega-conotoxin MVIIC
    • Protein or peptide: Voltage-dependent calcium channel subunit alpha-2/delta-1
    • Protein or peptide: Voltage-dependent P/Q-type calcium channel subunit alpha-1A
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
  • Ligand: CALCIUM ION
  • Ligand: CHOLESTEROL
  • Ligand: [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate
  • Ligand: CHOLESTEROL HEMISUCCINATE
  • Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine
Keywordsvoltage-gated calcium channel in complex with toxins / MEMBRANE PROTEIN
Function / homology
Function and homology information


regulation of membrane repolarization during action potential / Presynaptic depolarization and calcium channel opening / positive regulation of high voltage-gated calcium channel activity / calcium ion transmembrane transport via high voltage-gated calcium channel / membrane depolarization during bundle of His cell action potential / high voltage-gated calcium channel activity / L-type voltage-gated calcium channel complex / host cell presynaptic membrane / cardiac muscle cell action potential involved in contraction / NCAM1 interactions ...regulation of membrane repolarization during action potential / Presynaptic depolarization and calcium channel opening / positive regulation of high voltage-gated calcium channel activity / calcium ion transmembrane transport via high voltage-gated calcium channel / membrane depolarization during bundle of His cell action potential / high voltage-gated calcium channel activity / L-type voltage-gated calcium channel complex / host cell presynaptic membrane / cardiac muscle cell action potential involved in contraction / NCAM1 interactions / regulation of ventricular cardiac muscle cell membrane repolarization / calcium ion transport into cytosol / syntaxin binding / regulation of calcium ion transmembrane transport via high voltage-gated calcium channel / voltage-gated calcium channel complex / ion channel inhibitor activity / neuromuscular junction development / calcium ion import across plasma membrane / neuronal dense core vesicle / regulation of heart rate by cardiac conduction / response to amyloid-beta / regulation of calcium ion transport / calcium channel regulator activity / voltage-gated calcium channel activity / sarcoplasmic reticulum / cell projection / protein localization to plasma membrane / Regulation of insulin secretion / calcium ion transmembrane transport / modulation of chemical synaptic transmission / Adrenaline,noradrenaline inhibits insulin secretion / cellular response to amyloid-beta / calcium ion transport / T cell receptor signaling pathway / amyloid-beta binding / positive regulation of cytosolic calcium ion concentration / toxin activity / chemical synaptic transmission / neuronal cell body / synapse / extracellular exosome / extracellular region / membrane / nucleus / metal ion binding / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Voltage-dependent calcium channel, P/Q-type, alpha-1 A / Conotoxin, omega-type, conserved site / Omega-conotoxin family signature. / Voltage-dependent calcium channel, L-type, beta-3 subunit / Voltage-dependent L-type calcium channel subunit beta-3, SH3 domain / Voltage-dependent calcium channel, L-type, beta subunit / Voltage-dependent L-type calcium channel subunit beta-1-4, N-terminal A domain / Voltage gated calcium channel subunit beta domain 4Aa N terminal / VWA N-terminal / Voltage-dependent calcium channel, alpha-2/delta subunit, conserved region ...Voltage-dependent calcium channel, P/Q-type, alpha-1 A / Conotoxin, omega-type, conserved site / Omega-conotoxin family signature. / Voltage-dependent calcium channel, L-type, beta-3 subunit / Voltage-dependent L-type calcium channel subunit beta-3, SH3 domain / Voltage-dependent calcium channel, L-type, beta subunit / Voltage-dependent L-type calcium channel subunit beta-1-4, N-terminal A domain / Voltage gated calcium channel subunit beta domain 4Aa N terminal / VWA N-terminal / Voltage-dependent calcium channel, alpha-2/delta subunit, conserved region / : / VWA N-terminal / Neuronal voltage-dependent calcium channel alpha 2acd / Voltage-dependent calcium channel, alpha-1 subunit, IQ domain / : / Voltage gated calcium channel IQ domain / Voltage gated calcium channel IQ domain / Voltage-dependent calcium channel, alpha-1 subunit / Voltage-dependent L-type calcium channel, IQ-associated domain / Voltage-dependent L-type calcium channel, IQ-associated / Guanylate kinase/L-type calcium channel beta subunit / Guanylate kinase / Guanylate kinase homologues. / von Willebrand factor type A domain / von Willebrand factor (vWF) type A domain / VWFA domain profile. / Voltage-dependent channel domain superfamily / von Willebrand factor, type A / von Willebrand factor A-like domain superfamily / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain / Ion transport domain / Ion transport protein / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Voltage-dependent P/Q-type calcium channel subunit alpha-1A / Omega-conotoxin MVIIC / Voltage-dependent L-type calcium channel subunit beta-3 / Voltage-dependent calcium channel subunit alpha-2/delta-1
Similarity search - Component
Biological speciesHomo sapiens (human) / Conus magus (magus cone)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.11 Å
AuthorsYan N / Li Z / Cong Y / Wu T / Wang T
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32330052 China
CitationJournal: Cell Res / Year: 2024
Title: Structural basis for different ω-agatoxin IVA sensitivities of the P-type and Q-type Ca2.1 channels.
Authors: Zhangqiang Li / Ye Cong / Tong Wu / Tongtong Wang / Xinyao Lou / Xinyu Yang / Nieng Yan /
History
DepositionNov 29, 2023-
Header (metadata) releaseMar 20, 2024-
Map releaseMar 20, 2024-
UpdateOct 9, 2024-
Current statusOct 9, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_38159.png

Downloads & links

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Map

FileDownload / File: emd_38159.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.1 Å/pix.
x 320 pix.
= 351.328 Å		1.1 Å/pix.
x 320 pix.
= 351.328 Å		1.1 Å/pix.
x 320 pix.
= 351.328 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.0979 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.2
Minimum - Maximum-1.3078406 - 2.3011897
Average (Standard dev.)0.001576897 (±0.060204647)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 351.328 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_38159_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_38159_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Sample components

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Entire : Voltage-gated P/Q type calcium channel

EntireName: Voltage-gated P/Q type calcium channel
Components
  • Complex: Voltage-gated P/Q type calcium channel
    • Protein or peptide: Voltage-dependent L-type calcium channel subunit beta-3
    • Protein or peptide: Omega-conotoxin MVIIC
    • Protein or peptide: Voltage-dependent calcium channel subunit alpha-2/delta-1
    • Protein or peptide: Voltage-dependent P/Q-type calcium channel subunit alpha-1A
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
  • Ligand: CALCIUM ION
  • Ligand: CHOLESTEROL
  • Ligand: [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate
  • Ligand: CHOLESTEROL HEMISUCCINATE
  • Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine

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Supramolecule #1: Voltage-gated P/Q type calcium channel

SupramoleculeName: Voltage-gated P/Q type calcium channel / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Voltage-dependent L-type calcium channel subunit beta-3

MacromoleculeName: Voltage-dependent L-type calcium channel subunit beta-3
type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 56.23157 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MYDDSYVPGF EDSEAGSADS YTSRPSLDSD VSLEEDRESA RREVESQAQQ QLERAKHKPV AFAVRTNVSY CGVLDEECPV QGSGVNFEA KDFLHIKEKY SNDWWIGRLV KEGGDIAFIP SPQRLESIRL KQEQKARRSG NPSSLSDIGN RRSPPPSLAK Q KQKQAEHV ...String:
MYDDSYVPGF EDSEAGSADS YTSRPSLDSD VSLEEDRESA RREVESQAQQ QLERAKHKPV AFAVRTNVSY CGVLDEECPV QGSGVNFEA KDFLHIKEKY SNDWWIGRLV KEGGDIAFIP SPQRLESIRL KQEQKARRSG NPSSLSDIGN RRSPPPSLAK Q KQKQAEHV PPYDVVPSMR PVVLVGPSLK GYEVTDMMQK ALFDFLKHRF DGRISITRVT ADLSLAKRSV LNNPGKRTII ER SSARSSI AEVQSEIERI FELAKSLQLV VLDADTINHP AQLAKTSLAP IIVFVKVSSP KVLQRLIRSR GKSQMKHLTV QMM AYDKLV QCPPESFDVI LDENQLEDAC EHLAEYLEVY WRATHHPAPG PGLLGPPSAI PGLQNQQLLG ERGEEHSPLE RDSL MPSDE ASESSRQAWT GSSQRSSRHL EEDYADAYQD LYQPHRQHTS GLPSANGHDP QDRLLAQDSE HNHSDRNWQR NRPWP KDSY LEHHHHHHHH HH

UniProtKB: Voltage-dependent L-type calcium channel subunit beta-3

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Macromolecule #2: Omega-conotoxin MVIIC

MacromoleculeName: Omega-conotoxin MVIIC / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Conus magus (magus cone)
Molecular weightTheoretical: 2.763341 KDa
SequenceString:
CKGKGAPCRK TMYDCCSGSC GRRGKC

UniProtKB: Omega-conotoxin MVIIC

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Macromolecule #3: Voltage-dependent calcium channel subunit alpha-2/delta-1

MacromoleculeName: Voltage-dependent calcium channel subunit alpha-2/delta-1
type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 126.316148 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAAGCLLALT LTLFQSLLIG PSSEEPFPSA VTIKSWVDKM QEDLVTLAKT ASGVNQLVDI YEKYQDLYTV EPNNARQLVE IAARDIEKL LSNRSKALVR LALEAEKVQA AHQWREDFAS NEVVYYNAKD DLDPEKNDSE PGSQRIKPVF IEDANFGRQI S YQHAAVHI ...String:
MAAGCLLALT LTLFQSLLIG PSSEEPFPSA VTIKSWVDKM QEDLVTLAKT ASGVNQLVDI YEKYQDLYTV EPNNARQLVE IAARDIEKL LSNRSKALVR LALEAEKVQA AHQWREDFAS NEVVYYNAKD DLDPEKNDSE PGSQRIKPVF IEDANFGRQI S YQHAAVHI PTDIYEGSTI VLNELNWTSA LDEVFKKNRE EDPSLLWQVF GSATGLARYY PASPWVDNSR TPNKIDLYDV RR RPWYIQG AASPKDMLIL VDVSGSVSGL TLKLIRTSVS EMLETLSDDD FVNVASFNSN AQDVSCFQHL VQANVRNKKV LKD AVNNIT AKGITDYKKG FSFAFEQLLN YNVSRANCNK IIMLFTDGGE ERAQEIFNKY NKDKKVRVFT FSVGQHNYDR GPIQ WMACE NKGYYYEIPS IGAIRINTQE YLDVLGRPMV LAGDKAKQVQ WTNVYLDALE LGLVITGTLP VFNITGQFEN KTNLK NQLI LGVMGVDVSL EDIKRLTPRF TLCPNGYYFA IDPNGYVLLH PNLQPKPIGV GIPTINLRKR RPNIQNPKSQ EPVTLD FLD AELENDIKVE IRNKMIDGES GEKTFRTLVK SQDERYIDKG NRTYTWTPVN GTDYSLALVL PTYSFYYIKA KLEETIT QA RYSETLKPDN FEESGYTFIA PRDYCNDLKI SDNNTEFLLN FNEFIDRKTP NNPSCNADLI NRVLLDAGFT NELVQNYW S KQKNIKGVKA RFVVTDGGIT RVYPKEAGEN WQENPETYED SFYKRSLDND NYVFTAPYFN KSGPGAYESG IMVSKAVEI YIQGKLLKPA VVGIKIDVNS WIENFTKTSI RDPCAGPVCD CKRNSDVMDC VILDDGGFLL MANHDDYTNQ IGRFFGEIDP SLMRHLVNI SVYAFNKSYD YQSVCEPGAA PKQGAGHRSA YVPSVADILQ IGWWATAAAW SILQQFLLSL TFPRLLEAVE M EDDDFTAS LSKQSCITEQ TQYFFDNDSK SFSGVLDCGN CSRIFHGEKL MNTNLIFIMV ESKGTCPCDT RLLIQAEQTS DG PNPCDMV KQPRYRKGPD VCFDNNVLED YTDCGGVSGL NPSLWYIIGI QFLLLWLVSG STHRLLLEHH HHHHHHHH

UniProtKB: Voltage-dependent calcium channel subunit alpha-2/delta-1

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Macromolecule #4: Voltage-dependent P/Q-type calcium channel subunit alpha-1A

MacromoleculeName: Voltage-dependent P/Q-type calcium channel subunit alpha-1A
type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 287.534312 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MASWSHPQFE KGGGARGGSG GGSWSHPQFE KGFDYKDDDD KGTMARFGDE MPARYGGGGS GAAAGVVVGS GGGRGAGGSR QGGQPGAQR MYKQSMAQRA RTMALYNPIP VRQNCLTVNR SLFLFSEDNV VRKYAKKITE WPPFEYMILA TIIANCIVLA L EQHLPDDD ...String:
MASWSHPQFE KGGGARGGSG GGSWSHPQFE KGFDYKDDDD KGTMARFGDE MPARYGGGGS GAAAGVVVGS GGGRGAGGSR QGGQPGAQR MYKQSMAQRA RTMALYNPIP VRQNCLTVNR SLFLFSEDNV VRKYAKKITE WPPFEYMILA TIIANCIVLA L EQHLPDDD KTPMSERLDD TEPYFIGIFC FEAGIKIIAL GFAFHKGSYL RNGWNVMDFV VVLTGILATV GTEFDLRTLR AV RVLRPLK LVSGIPSLQV VLKSIMKAMI PLLQIGLLLF FAILIFAIIG LEFYMGKFHT TCFEEGTDDI QGESPAPCGT EEP ARTCPN GTKCQPYWEG PNNGITQFDN ILFAVLTVFQ CITMEGWTDL LYNSNDASGN TWNWLYFIPL IIIGSFFMLN LVLG VLSGE FAKERERVEN RRAFLKLRRQ QQIERELNGY MEWISKAEEV ILAEDETDGE QRHPFDALRR TTIKKSKTDL LNPEE AEDQ LADIASVGSP FARASIKSAK LENSTFFHKK ERRMRFYIRR MVKTQAFYWT VLSLVALNTL CVAIVHYNQP EWLSDF LYY AEFIFLGLFM SEMFIKMYGL GTRPYFHSSF NCFDCGVIIG SIFEVIWAVI KPGTSFGISV LRALRLLRIF KVTKYWA SL RNLVVSLLNS MKSIISLLFL LFLFIVVFAL LGMQLFGGQF NFDEGTPPTN FDTFPAAIMT VFQILTGEDW NEVMYDGI K SQGGVQGGMV FSIYFIVLTL FGNYTLLNVF LAIAVDNLAN AQELTKDEQE EEEAANQKLA LQKAKEVAEV SPLSAANMS IAVKEQQKNQ KPAKSVWEQR TSEMRKQNLL ASREALYNEM DPDERWKAAY TRHLRPDMKT HLDRPLVVDP QENRNNNTNK SRAAEPTVD QRLGQQRAED FLRKQARYHD RARDPSGSAG LDARRPWAGS QEAELSREGP YGRESDHHAR EGSLEQPGFW E GEAERGKA GDPHRRHVHR QGGSRESRSG SPRTGADGEH RRHRAHRRPG EEGPEDKAER RARHREGSRP ARGGEGEGEG PD GGERRRR HRHGAPATYE GDARREDKER RHRRRKENQG SGVPVSGPNL STTRPIQQDL GRQDPPLAED IDNMKNNKLA TAE SAAPHG SLGHAGLPQS PAKMGNSTDP GPMLAIPAMA TNPQNAASRR TPNNPGNPSN PGPPKTPENS LIVTNPSGTQ TNSA KTARK PDHTTVDIPP ACPPPLNHTV VQVNKNANPD PLPKKEEEKK EEEEDDRGED GPKPMPPYSS MFILSTTNPL RRLCH YILN LRYFEMCILM VIAMSSIALA AEDPVQPNAP RNNVLRYFDY VFTGVFTFEM VIKMIDLGLV LHQGAYFRDL WNILDF IVV SGALVAFAFT GNSKGKDINT IKSLRVLRVL RPLKTIKRLP KLKAVFDCVV NSLKNVFNIL IVYMLFMFIF AVVAVQL FK GKFFHCTDES KEFEKDCRGK YLLYEKNEVK ARDREWKKYE FHYDNVLWAL LTLFTVSTGE GWPQVLKHSV DATFENQG P SPGYRMEMSI FYVVYFVVFP FFFVNIFVAL IIITFQEQGD KMMEEYSLEK NERACIDFAI SAKPLTRHMP QNKQSFQYR MWQFVVSPPF EYTIMAMIAL NTIVLMMKFY GASVAYENAL RVFNIVFTSL FSLECVLKVM AFGILNYFRD AWNIFDFVTV LGSITDILV TEFGNNFINL SFLRLFRAAR LIKLLRQGYT IRILLWTFVQ SFKALPYVCL LIAMLFFIYA IIGMQVFGNI G IDVEDEDS DEDEFQITEH NNFRTFFQAL MLLFRSATGE AWHNIMLSCL SGKPCDKNSG ILTRECGNEF AYFYFVSFIF LC SFLMLNL FVAVIMDNFE YLTRDSSILG PHHLDEYVRV WAEYDPAAWG RMPYLDMYQM LRHMSPPLGL GKKCPARVAY KRL LRMDLP VADDNTVHFN STLMALIRTA LDIKIAKGGA DKQQMDAELR KEMMAIWPNL SQKTLDLLVT PHKSTDLTVG KIYA AMMIM EYYRQSKAKK LQAMREEQDR TPLMFQRMEP PSPTQEGGPG QNALPSTQLD PGGALMAHES GLKESPSWVT QRAQE MFQK TGTWSPEQGP PTDMPNSQPN SQSVEMREMG RDGYSDSEHY LPMEGQGRAA SMPRLPAENQ RRRGRPRGNN LSTISD TSP MKRSASVLGP KARRLDDYSL ERVPPEENQR HHQRRRDRSH RASERSLGRY TDVDTGLGTD LSMTTQSGDL PSKERDQ ER GRPKDRKHRQ HHHHHHHHHH PPPPDKDRYA QERPDHGRAR ARDQRWSRSP SEGREHMAHR QGSSSVSGSP APSTSGTS T PRRGRRQLPQ TPSTPRPHVS YSPVIRKAGG SGPPQQQQQQ QQQQQQQAVA RPGRAATSGP RRYPGPTAEP LAGDRPPTG GHSSGRSPRM ERRVPGPARS ESPRACRHGG ARWPASGPHV SEGPPGPRHH GYYRGSDYDE ADGPGSGGGE EAMAGAYDAP PPVRHASSG ATGRSPRTPR ASGPACASPS RHGRRLPNGY YPAHGLARPR GPGSRKGLHE PYSESDDDWC

UniProtKB: Voltage-dependent P/Q-type calcium channel subunit alpha-1A

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Macromolecule #8: 2-acetamido-2-deoxy-beta-D-glucopyranose

MacromoleculeName: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 8 / Number of copies: 2 / Formula: NAG
Molecular weightTheoretical: 221.208 Da
Chemical component information

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

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Macromolecule #9: CALCIUM ION

MacromoleculeName: CALCIUM ION / type: ligand / ID: 9 / Number of copies: 3 / Formula: CA
Molecular weightTheoretical: 40.078 Da

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Macromolecule #10: CHOLESTEROL

MacromoleculeName: CHOLESTEROL / type: ligand / ID: 10 / Number of copies: 5 / Formula: CLR
Molecular weightTheoretical: 386.654 Da
Chemical component information

ChemComp-CLR:
CHOLESTEROL

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Macromolecule #11: [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tr...

MacromoleculeName: [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate
type: ligand / ID: 11 / Number of copies: 1 / Formula: PT5
Molecular weightTheoretical: 1.047088 KDa

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Macromolecule #12: CHOLESTEROL HEMISUCCINATE

MacromoleculeName: CHOLESTEROL HEMISUCCINATE / type: ligand / ID: 12 / Number of copies: 3 / Formula: Y01
Molecular weightTheoretical: 486.726 Da
Chemical component information

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

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Macromolecule #13: 1,2-Distearoyl-sn-glycerophosphoethanolamine

MacromoleculeName: 1,2-Distearoyl-sn-glycerophosphoethanolamine / type: ligand / ID: 13 / Number of copies: 2 / Formula: 3PE
Molecular weightTheoretical: 748.065 Da
Chemical component information

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.3 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.11 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 126389
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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