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Yorodumi- PDB-8x83: The cryo-EM structure of insect gustatory receptor Gr43a I418A fr... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8x83 | ||||||
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Title | The cryo-EM structure of insect gustatory receptor Gr43a I418A from Drosophila melanogaster in complex with fructose | ||||||
Components | Gustatory receptor for sugar taste 43a | ||||||
Keywords | MEMBRANE PROTEIN / gustatory receptor / ligand-gated ion channel / Gr43a / fructose | ||||||
Function / homology | Function and homology information sweet taste receptor activity / regulation of appetite / male courtship behavior / taste receptor activity / adult feeding behavior / chemosensory behavior / sensory perception of sweet taste / regulation of feeding behavior / cellular response to fructose stimulus / ligand-gated monoatomic cation channel activity ...sweet taste receptor activity / regulation of appetite / male courtship behavior / taste receptor activity / adult feeding behavior / chemosensory behavior / sensory perception of sweet taste / regulation of feeding behavior / cellular response to fructose stimulus / ligand-gated monoatomic cation channel activity / monoatomic cation transmembrane transport / sensory perception of taste / axon / neuronal cell body / dendrite / signal transduction / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.8 Å | ||||||
Authors | Ma, D. / Guo, J. | ||||||
Funding support | China, 1items
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Citation | Journal: Science / Year: 2024 Title: Structural basis for sugar perception by gustatory receptors. Authors: Demin Ma / Meiqin Hu / Xiaotong Yang / Qiang Liu / Fan Ye / Weijie Cai / Yong Wang / Ximing Xu / Shenghai Chang / Ruiying Wang / Wei Yang / Sheng Ye / Nannan Su / Minrui Fan / Haoxing Xu / Jiangtao Guo / Abstract: Insects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures of sweet taste ...Insects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures of sweet taste receptors GR43a and GR64a in the apo and sugar-bound states. Both GRs form tetrameric sugar-gated cation channels composed of one central pore domain (PD) and four peripheral ligand-binding domains (LBDs). Whereas GR43a is specifically activated by the monosaccharide fructose that binds to a narrow pocket in LBDs, disaccharides sucrose and maltose selectively activate GR64a by binding to a larger and flatter pocket in LBDs. Sugar binding to LBDs induces local conformational changes, which are subsequently transferred to the PD to cause channel opening. Our studies reveal a structural basis for sugar recognition and activation of GRs. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8x83.cif.gz | 571.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8x83.ent.gz | 480.5 KB | Display | PDB format |
PDBx/mmJSON format | 8x83.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8x83_validation.pdf.gz | 565 KB | Display | wwPDB validaton report |
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Full document | 8x83_full_validation.pdf.gz | 575.6 KB | Display | |
Data in XML | 8x83_validation.xml.gz | 28.7 KB | Display | |
Data in CIF | 8x83_validation.cif.gz | 42.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/x8/8x83 ftp://data.pdbj.org/pub/pdb/validation_reports/x8/8x83 | HTTPS FTP |
-Related structure data
Related structure data | 38134MC 8jm9C 8jmaC 8jmeC 8jmhC 8jmiC 8x82C 8x84C M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 49267.312 Da / Num. of mol.: 4 / Mutation: I418A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: Gr43a, GR43.1, CG1712 / Production host: Homo sapiens (human) / References: UniProt: Q9V4K2 #2: Chemical | #3: Sugar | ChemComp-FRU / #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: gustatory receptor Gr43a I418A / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: #1 / Source: RECOMBINANT |
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Source (natural) | Organism: Drosophila melanogaster (fruit fly) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 7.4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 1500 nm / Nominal defocus min: 800 nm |
Image recording | Electron dose: 52 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3D reconstruction | Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 158079 / Symmetry type: POINT |