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Open data
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Basic information
Entry | Database: PDB / ID: 8wrz | ||||||||||||||||||
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Title | Cry-EM structure of cannabinoid receptor-arrestin 2 complex | ||||||||||||||||||
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![]() | MEMBRANE PROTEIN / complex / GPCR | ||||||||||||||||||
Function / homology | ![]() renal water retention / Defective AVP does not bind AVPR2 and causes neurohypophyseal diabetes insipidus (NDI) / Vasopressin-like receptors / cannabinoid signaling pathway / regulation of penile erection / retrograde trans-synaptic signaling by endocannabinoid / regulation of systemic arterial blood pressure by vasopressin / vasopressin receptor activity / cannabinoid receptor activity / angiotensin receptor binding ...renal water retention / Defective AVP does not bind AVPR2 and causes neurohypophyseal diabetes insipidus (NDI) / Vasopressin-like receptors / cannabinoid signaling pathway / regulation of penile erection / retrograde trans-synaptic signaling by endocannabinoid / regulation of systemic arterial blood pressure by vasopressin / vasopressin receptor activity / cannabinoid receptor activity / angiotensin receptor binding / negative regulation of mast cell activation / trans-synaptic signaling by endocannabinoid, modulating synaptic transmission / negative regulation of fatty acid beta-oxidation / negative regulation of dopamine secretion / positive regulation of acute inflammatory response to antigenic stimulus / regulation of feeding behavior / negative regulation of serotonin secretion / Activation of SMO / regulation of presynaptic cytosolic calcium ion concentration / hemostasis / telencephalon development / negative regulation of action potential / positive regulation of systemic arterial blood pressure / negative regulation of interleukin-8 production / Class A/1 (Rhodopsin-like receptors) / positive regulation of blood pressure / positive regulation of fever generation / regulation of metabolic process / arrestin family protein binding / G protein-coupled receptor internalization / axonal fasciculation / enzyme inhibitor activity / positive regulation of intracellular signal transduction / Lysosome Vesicle Biogenesis / regulation of synaptic transmission, GABAergic / positive regulation of Rho protein signal transduction / Golgi Associated Vesicle Biogenesis / negative regulation of NF-kappaB transcription factor activity / stress fiber assembly / negative regulation of Notch signaling pathway / pseudopodium / regulation of insulin secretion / negative regulation of interleukin-6 production / positive regulation of receptor internalization / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / endocytic vesicle / GABA-ergic synapse / maternal process involved in female pregnancy / clathrin-coated pit / positive regulation of vasoconstriction / regulation of synaptic transmission, glutamatergic / cellular response to hormone stimulus / negative regulation of protein ubiquitination / activation of adenylate cyclase activity / visual perception / negative regulation of blood pressure / Activated NOTCH1 Transmits Signal to the Nucleus / response to nutrient / GTPase activator activity / response to cocaine / response to cytokine / G protein-coupled receptor activity / G protein-coupled receptor binding / electron transport chain / peptide binding / insulin-like growth factor receptor binding / Signaling by high-kinase activity BRAF mutants / clathrin-coated endocytic vesicle membrane / response to nicotine / MAP2K and MAPK activation / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / cytoplasmic vesicle membrane / adenylate cyclase-activating G protein-coupled receptor signaling pathway / memory / Vasopressin regulates renal water homeostasis via Aquaporins / positive regulation of neuron projection development / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / Signaling by BRAF and RAF1 fusions / protein transport / actin cytoskeleton / Thrombin signalling through proteinase activated receptors (PARs) / Cargo recognition for clathrin-mediated endocytosis / glucose homeostasis / Clathrin-mediated endocytosis / presynaptic membrane / G alpha (i) signalling events / growth cone / cytoplasmic vesicle / G alpha (s) signalling events / ubiquitin-dependent protein catabolic process / spermatogenesis / proteasome-mediated ubiquitin-dependent protein catabolic process / response to ethanol / mitochondrial outer membrane / response to lipopolysaccharide / periplasmic space / transcription coactivator activity Similarity search - Function | ||||||||||||||||||
Biological species | ![]() Phage display vector pTDisp (others) ![]() ![]() | ||||||||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.6 Å | ||||||||||||||||||
![]() | Wang, Y.X. / Wang, T. / Wu, L.J. / Hua, T. / Liu, Z.J. | ||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structure of cannabinoid receptor CB1-β-arrestin complex. Authors: Yuxia Wang / Lijie Wu / Tian Wang / Junlin Liu / Fei Li / Longquan Jiang / Zhongbo Fan / Yanan Yu / Na Chen / Qianqian Sun / Qiwen Tan / Tian Hua / Zhi-Jie Liu / ![]() | ||||||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 175.7 KB | Display | ![]() |
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PDB format | ![]() | 131.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 38.6 KB | Display | |
Data in CIF | ![]() | 55.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 37795MC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
#1: Protein | Mass: 44271.508 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Antibody | Mass: 28693.922 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Phage display vector pTDisp (others) / Production host: ![]() ![]() |
#3: Protein | Mass: 53577.543 Da / Num. of mol.: 1 / Mutation: M29W, H124I, T210I, E273K, T283V, R340E Source method: isolated from a genetically manipulated source Details: The chimera of Cytochrome b-562, linker, and Cannabinoid receptor 1 Source: (gene. exp.) ![]() ![]() ![]() Gene: cybC, CNR1 / Production host: ![]() |
#4: Protein/peptide | Mass: 2920.651 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#5: Chemical | ChemComp-8D0 / ( |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Cry-EM structure of cannabinoid receptor-arrestin 2 complex Type: COMPLEX / Entity ID: #1-#4 / Source: MULTIPLE SOURCES |
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Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 7.5 |
Specimen | Conc.: 1.5 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 60 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Num. of real images: 25847 |
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Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 130058 / Symmetry type: POINT | ||||||||||||||||||||||||
Atomic model building | Protocol: RIGID BODY FIT | ||||||||||||||||||||||||
Refine LS restraints |
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