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Open data
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Basic information
Entry | Database: PDB / ID: 8vt2 | ||||||
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Title | cryo-EM structure of HMPV (MPV-2c) | ||||||
![]() | Prefusion of HMPV (MPV-2c) | ||||||
![]() | VIRAL PROTEIN / Prefusion / HMPV / Cryo-EM / VIRUS | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.14 Å | ||||||
![]() | Yu, X. / Langedijk, J.P.M. | ||||||
Funding support | 1items
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![]() | ![]() Title: Efficacious human metapneumovirus vaccine based on AI-guided engineering of a closed prefusion trimer Authors: Bakkers, M.J.G. / Ritschel, T. / Tiemessen, M. / Dijkman, J. / Zuffiano, A.A. / Yu, X. / Overveld, D. / Le, L. / Voorzaat, R. / Haaren, M. / Man, M. / Sharma, S. / Tamara, S. / Fits, L. / ...Authors: Bakkers, M.J.G. / Ritschel, T. / Tiemessen, M. / Dijkman, J. / Zuffiano, A.A. / Yu, X. / Overveld, D. / Le, L. / Voorzaat, R. / Haaren, M. / Man, M. / Sharma, S. / Tamara, S. / Fits, L. / Zahn, R. / Juraszek, J. / Langedijk, J.P.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 243.2 KB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.8 MB | Display | ![]() |
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Full document | ![]() | 1.8 MB | Display | |
Data in XML | ![]() | 46.3 KB | Display | |
Data in CIF | ![]() | 69.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein | Mass: 56553.586 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Polysaccharide | Source method: isolated from a genetically manipulated source #3: Polysaccharide | Source method: isolated from a genetically manipulated source #4: Sugar | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Prefusion of HMPV (MPV-2cREKR) / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
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Microscopy | Model: FEI TALOS ARCTICA |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2400 nm / Nominal defocus min: 1200 nm |
Image recording | Electron dose: 40 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k) |
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Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 3.14 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 97386 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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