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-基本情報
登録情報 | データベース: PDB / ID: 8tk8 | ||||||||||||
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タイトル | Human Type 3 IP3 Receptor - Resting State (+IP3/ATP) | ||||||||||||
要素 | Inositol 1,4,5-trisphosphate receptor type 3 | ||||||||||||
キーワード | TRANSPORT PROTEIN / Ion Channel / Calcium Channel / Endoplasmic Reticulum | ||||||||||||
機能・相同性 | 機能・相同性情報 DAG and IP3 signaling / sensory perception of bitter taste / platelet dense tubular network membrane / inositol 1,3,4,5 tetrakisphosphate binding / sensory perception of umami taste / inositol 1,4,5-trisphosphate-gated calcium channel activity / sensory perception of sweet taste / Effects of PIP2 hydrolysis / Elevation of cytosolic Ca2+ levels / PLC beta mediated events ...DAG and IP3 signaling / sensory perception of bitter taste / platelet dense tubular network membrane / inositol 1,3,4,5 tetrakisphosphate binding / sensory perception of umami taste / inositol 1,4,5-trisphosphate-gated calcium channel activity / sensory perception of sweet taste / Effects of PIP2 hydrolysis / Elevation of cytosolic Ca2+ levels / PLC beta mediated events / inositol hexakisphosphate binding / inositol 1,4,5 trisphosphate binding / nuclear outer membrane / CLEC7A (Dectin-1) induces NFAT activation / transport vesicle membrane / cytoplasmic side of endoplasmic reticulum membrane / intracellularly gated calcium channel activity / brush border / Role of phospholipids in phagocytosis / calcium ion homeostasis / Ion homeostasis / release of sequestered calcium ion into cytosol / FCERI mediated Ca+2 mobilization / FCGR3A-mediated IL10 synthesis / phosphatidylinositol binding / sarcoplasmic reticulum / secretory granule membrane / VEGFR2 mediated cell proliferation / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / Regulation of insulin secretion / long-term synaptic potentiation / memory / Sensory perception of sweet, bitter, and umami (glutamate) taste / response to calcium ion / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / sensory perception of taste / apical part of cell / myelin sheath / Ca2+ pathway / positive regulation of cytosolic calcium ion concentration / protein homotetramerization / receptor complex / G protein-coupled receptor signaling pathway / neuronal cell body / calcium ion binding / endoplasmic reticulum membrane / nucleolus / endoplasmic reticulum / nucleoplasm / membrane / plasma membrane / cytoplasm 類似検索 - 分子機能 | ||||||||||||
生物種 | Homo sapiens (ヒト) | ||||||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.7 Å | ||||||||||||
データ登録者 | Paknejad, N. / Sapuru, V. / Hite, R.K. | ||||||||||||
資金援助 | 米国, 3件
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引用 | ジャーナル: Nat Commun / 年: 2023 タイトル: Structural titration reveals Ca-dependent conformational landscape of the IP receptor. 著者: Navid Paknejad / Vinay Sapuru / Richard K Hite / 要旨: Inositol 1,4,5-trisphosphate receptors (IPRs) are endoplasmic reticulum Ca channels whose biphasic dependence on cytosolic Ca gives rise to Ca oscillations that regulate fertilization, cell division ...Inositol 1,4,5-trisphosphate receptors (IPRs) are endoplasmic reticulum Ca channels whose biphasic dependence on cytosolic Ca gives rise to Ca oscillations that regulate fertilization, cell division and cell death. Despite the critical roles of IPR-mediated Ca responses, the structural underpinnings of the biphasic Ca dependence that underlies Ca oscillations are incompletely understood. Here, we collect cryo-EM images of an IPR with Ca concentrations spanning five orders of magnitude. Unbiased image analysis reveals that Ca binding does not explicitly induce conformational changes but rather biases a complex conformational landscape consisting of resting, preactivated, activated, and inhibited states. Using particle counts as a proxy for relative conformational free energy, we demonstrate that Ca binding at a high-affinity site allows IPRs to activate by escaping a low-energy resting state through an ensemble of preactivated states. At high Ca concentrations, IPRs preferentially enter an inhibited state stabilized by a second, low-affinity Ca binding site. Together, these studies provide a mechanistic basis for the biphasic Ca-dependence of IPR channel activity. | ||||||||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 8tk8.cif.gz | 4.2 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb8tk8.ent.gz | 表示 | PDB形式 | |
PDBx/mmJSON形式 | 8tk8.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 8tk8_validation.pdf.gz | 2.1 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 8tk8_full_validation.pdf.gz | 2.1 MB | 表示 | |
XML形式データ | 8tk8_validation.xml.gz | 210.8 KB | 表示 | |
CIF形式データ | 8tk8_validation.cif.gz | 321.6 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/tk/8tk8 ftp://data.pdbj.org/pub/pdb/validation_reports/tk/8tk8 | HTTPS FTP |
-関連構造データ
関連構造データ | 41323MC 8tkdC 8tkeC 8tkfC 8tkgC 8tkhC 8tkiC 8tl9C 8tlaC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
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非結晶学的対称性 (NCS) | NCSドメイン:
NCSドメイン領域: Ens-ID: ens_1
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