[English] 日本語
![](img/lk-miru.gif)
- PDB-8tem: Cryo-EM structure of Arabidopsis thaliana Bor1 mutant (R637E/E641... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 8tem | ||||||
---|---|---|---|---|---|---|---|
Title | Cryo-EM structure of Arabidopsis thaliana Bor1 mutant (R637E/E641R/R643E) in the inward-facing conformation in lauryl maltose neopentyl glycol (LMNG) | ||||||
![]() | Boron transporter 1 | ||||||
![]() | TRANSPORT PROTEIN / Borate transporter / Bor1 | ||||||
Function / homology | ![]() borate transmembrane transport / response to boron-containing substance / cellular response to boron-containing substance levels / active borate transmembrane transporter activity / monoatomic ion homeostasis / solute:inorganic anion antiporter activity / monoatomic anion transport / vacuolar membrane / transmembrane transport / endosome membrane / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.02 Å | ||||||
![]() | Jiang, Y. / Jiang, J. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: Structure of borate transporter Bor1 reveals a novel auto-inhibition mechanism for the SLC4 family Authors: Jiang, Y. / Jiang, J. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 208 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 163.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.4 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1.4 MB | Display | |
Data in XML | ![]() | 42.3 KB | Display | |
Data in CIF | ![]() | 62.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 41191MC ![]() 8tegC ![]() 8tehC ![]() 8teiC ![]() 8tejC ![]() 8telC ![]() 8tenC M: map data used to model this data C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
-
Assembly
Deposited unit | ![]()
|
---|---|
1 |
|
-
Components
#1: Protein | Mass: 80108.898 Da / Num. of mol.: 2 / Fragment: R637E/E641R/R643E Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-
Sample preparation
Component | Name: Arabidopsis thaliana Boron transporter 1 / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
---|---|
Molecular weight | Experimental value: NO |
Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 6.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: OTHER / Accelerating voltage: 300 kV / Illumination mode: OTHER |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 800 nm |
Image recording | Electron dose: 55 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
-
Processing
EM software |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
Symmetry | Point symmetry: C2 (2 fold cyclic) | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.02 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 80443 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
|