GroEL-GroES complex / chaperonin ATPase / mitochondrial unfolded protein response / protein import into mitochondrial intermembrane space / virion assembly / chaperone cofactor-dependent protein refolding / positive regulation of interferon-alpha production / isomerase activity / ATP-dependent protein folding chaperone / response to radiation ...GroEL-GroES complex / chaperonin ATPase / mitochondrial unfolded protein response / protein import into mitochondrial intermembrane space / virion assembly / chaperone cofactor-dependent protein refolding / positive regulation of interferon-alpha production / isomerase activity / ATP-dependent protein folding chaperone / response to radiation / positive regulation of interleukin-6 production / positive regulation of type II interferon production / unfolded protein binding / protein folding / positive regulation of T cell activation / protein-folding chaperone binding / response to heat / protein refolding / magnesium ion binding / ATP hydrolysis activity / ATP binding / identical protein binding / membrane / cytosol 類似検索 - 分子機能
平均露光時間: 16.67 sec. / 電子線照射量: 40 e/Å2 / 検出モード: COUNTING フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 撮影したグリッド数: 1 / 実像数: 4672
画像スキャン
横: 4096 / 縦: 4096
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解析
EMソフトウェア
ID
名称
バージョン
カテゴリ
1
cryoSPARC
4.3
粒子像選択
2
EPU
3.4
画像取得
4
cryoSPARC
4.3
CTF補正
9
cryoSPARC
4.4
初期オイラー角割当
10
cryoSPARC
4.4
最終オイラー角割当
11
cryoSPARC
4.4
分類
12
cryoSPARC
4.4
3次元再構成
19
Coot
0.9.8.5
モデル精密化
20
PHENIX
1.21.5207
モデル精密化
CTF補正
タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION
粒子像の選択
選択した粒子像数: 350383
対称性
点対称性: D7 (2回x7回 2面回転対称)
3次元再構成
解像度: 2.45 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 203031 / アルゴリズム: FOURIER SPACE / クラス平均像の数: 40 / 対称性のタイプ: POINT
原子モデル構築
プロトコル: AB INITIO MODEL / 空間: REAL / Target criteria: 0.88 詳細: The C-alpha model was obtained by two rounds of ab initio modeling using machine learning-based ModelAngelo 1.09 software. The incorrectly assigned GroEL residues were then manually corrected. ...詳細: The C-alpha model was obtained by two rounds of ab initio modeling using machine learning-based ModelAngelo 1.09 software. The incorrectly assigned GroEL residues were then manually corrected. The C-alpha trace of the GroTAC peptide was initially constructed by ModelAngelo, with residue identities suggested through a comparison to the 1MNF PDB structure. The reconstruction to full-atom was performed using cg2all 1.5 software. The first stage of refinement was done in Coot (0.9.8.5). The final refinement stage involved two rounds of phenix.real_space_refine (Phenix 1.21.5207).
原子モデル構築
詳細: The CA model was obtained by ModelAngelo (1.09) and then subsequently rebuilt to full-atom by cg2all (1.5). Source name: Other / タイプ: in silico model