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- PDB-8rqe: Composite map of bacteriophage JBD30 capsid - neck complex -

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Basic information

Entry
Database: PDB / ID: 8rqe
TitleComposite map of bacteriophage JBD30 capsid - neck complex
Components
  • Bacteriophage Mu GpT domain-containing protein
  • DUF1320 domain-containing protein
  • Mu-like prophage FluMu N-terminal domain-containing protein
  • Portal protein
  • Stopper protein
  • Virion structural protein
KeywordsVIRAL PROTEIN / bacteriophage JBD30 / virion / capsid / connector / neck / portal / adaptor / stopper / tail
Function / homology
Function and homology information


Protein of unknown function UCP028589 / Protein of unknown function DUF935 / Portal protein of Mu bacteriophage / Bacteriophage Mu, Gene product J / Bacteriophage Mu, Gp36 / Bacteriophage Mu, GpT / Mu-like prophage major head subunit gpT
Similarity search - Domain/homology
Portal protein / Uncharacterized protein / Bacteriophage Mu GpT domain-containing protein / Mu-like prophage FluMu N-terminal domain-containing protein / Virion structural protein / DUF1320 domain-containing protein
Similarity search - Component
Biological speciesPseudomonas phage JBD30 (virus)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 8 Å
AuthorsValentova, L. / Fuzik, T. / Plevka, P.
Funding support Czech Republic, European Union, 2items
OrganizationGrant numberCountry
Ministry of Education, Youth and Sports of the Czech RepublicLX22NPO5103 Czech Republic
European Research Council (ERC)101043452European Union
Citation
Journal: EMBO J / Year: 2024
Title: Structure and replication of Pseudomonas aeruginosa phage JBD30.
Authors: Lucie Valentová / Tibor Füzik / Jiří Nováček / Zuzana Hlavenková / Jakub Pospíšil / Pavel Plevka /
Abstract: Bacteriophages are the most abundant biological entities on Earth, but our understanding of many aspects of their lifecycles is still incomplete. Here, we have structurally analysed the infection ...Bacteriophages are the most abundant biological entities on Earth, but our understanding of many aspects of their lifecycles is still incomplete. Here, we have structurally analysed the infection cycle of the siphophage Casadabanvirus JBD30. Using its baseplate, JBD30 attaches to Pseudomonas aeruginosa via the bacterial type IV pilus, whose subsequent retraction brings the phage to the bacterial cell surface. Cryo-electron microscopy structures of the baseplate-pilus complex show that the tripod of baseplate receptor-binding proteins attaches to the outer bacterial membrane. The tripod and baseplate then open to release three copies of the tape-measure protein, an event that is followed by DNA ejection. JBD30 major capsid proteins assemble into procapsids, which expand by 7% in diameter upon filling with phage dsDNA. The DNA-filled heads are finally joined with 180-nm-long tails, which bend easily because flexible loops mediate contacts between the successive discs of major tail proteins. It is likely that the structural features and replication mechanisms described here are conserved among siphophages that utilize the type IV pili for initial cell attachment.
#1: Journal: Embo J. / Year: 2024
Title: Structure and replication of Pseudomonas aeruginosa phage JBD30
Authors: Valentova, L. / Plevka, P. / Fuzik, T. / Novacek, J. / Pospisil, J.
History
DepositionJan 18, 2024Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 14, 2024Provider: repository / Type: Initial release
Revision 1.1Aug 28, 2024Group: Data collection / Database references / Category: citation / citation_author / em_admin / Item: _em_admin.last_update
Revision 1.2Oct 16, 2024Group: Data collection / Database references / Structure summary
Category: citation / citation_author ...citation / citation_author / em_admin / pdbx_entry_details
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID / _em_admin.last_update

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
aa: Mu-like prophage FluMu N-terminal domain-containing protein
ab: Mu-like prophage FluMu N-terminal domain-containing protein
ac: Mu-like prophage FluMu N-terminal domain-containing protein
ad: Mu-like prophage FluMu N-terminal domain-containing protein
ae: Mu-like prophage FluMu N-terminal domain-containing protein
af: Mu-like prophage FluMu N-terminal domain-containing protein
ag: Bacteriophage Mu GpT domain-containing protein
ah: Bacteriophage Mu GpT domain-containing protein
ai: Bacteriophage Mu GpT domain-containing protein
aj: Bacteriophage Mu GpT domain-containing protein
ak: Bacteriophage Mu GpT domain-containing protein
al: Bacteriophage Mu GpT domain-containing protein
am: Bacteriophage Mu GpT domain-containing protein
an: Mu-like prophage FluMu N-terminal domain-containing protein
ao: Mu-like prophage FluMu N-terminal domain-containing protein
ap: Mu-like prophage FluMu N-terminal domain-containing protein
aq: Mu-like prophage FluMu N-terminal domain-containing protein
ar: Mu-like prophage FluMu N-terminal domain-containing protein
as: Mu-like prophage FluMu N-terminal domain-containing protein
at: Mu-like prophage FluMu N-terminal domain-containing protein
au: Bacteriophage Mu GpT domain-containing protein
av: Bacteriophage Mu GpT domain-containing protein
aw: Bacteriophage Mu GpT domain-containing protein
ax: Bacteriophage Mu GpT domain-containing protein
ay: Bacteriophage Mu GpT domain-containing protein
az: Bacteriophage Mu GpT domain-containing protein
aA: Mu-like prophage FluMu N-terminal domain-containing protein
aB: Mu-like prophage FluMu N-terminal domain-containing protein
aC: Mu-like prophage FluMu N-terminal domain-containing protein
aD: Mu-like prophage FluMu N-terminal domain-containing protein
aE: Mu-like prophage FluMu N-terminal domain-containing protein
aF: Mu-like prophage FluMu N-terminal domain-containing protein
aG: Mu-like prophage FluMu N-terminal domain-containing protein
aH: Bacteriophage Mu GpT domain-containing protein
aI: Bacteriophage Mu GpT domain-containing protein
aJ: Bacteriophage Mu GpT domain-containing protein
aK: Bacteriophage Mu GpT domain-containing protein
aL: Bacteriophage Mu GpT domain-containing protein
aM: Bacteriophage Mu GpT domain-containing protein
aN: Bacteriophage Mu GpT domain-containing protein
aO: Mu-like prophage FluMu N-terminal domain-containing protein
aP: Mu-like prophage FluMu N-terminal domain-containing protein
aQ: Mu-like prophage FluMu N-terminal domain-containing protein
aR: Mu-like prophage FluMu N-terminal domain-containing protein
aS: Mu-like prophage FluMu N-terminal domain-containing protein
aT: Mu-like prophage FluMu N-terminal domain-containing protein
aU: Mu-like prophage FluMu N-terminal domain-containing protein
aV: Bacteriophage Mu GpT domain-containing protein
aW: Bacteriophage Mu GpT domain-containing protein
aX: Bacteriophage Mu GpT domain-containing protein
aY: Bacteriophage Mu GpT domain-containing protein
aZ: Bacteriophage Mu GpT domain-containing protein
ba: Bacteriophage Mu GpT domain-containing protein
bb: Bacteriophage Mu GpT domain-containing protein
bc: Mu-like prophage FluMu N-terminal domain-containing protein
bd: Mu-like prophage FluMu N-terminal domain-containing protein
be: Mu-like prophage FluMu N-terminal domain-containing protein
bf: Mu-like prophage FluMu N-terminal domain-containing protein
bg: Mu-like prophage FluMu N-terminal domain-containing protein
bh: Mu-like prophage FluMu N-terminal domain-containing protein
bi: Mu-like prophage FluMu N-terminal domain-containing protein
bj: Bacteriophage Mu GpT domain-containing protein
bk: Bacteriophage Mu GpT domain-containing protein
bl: Bacteriophage Mu GpT domain-containing protein
bm: Bacteriophage Mu GpT domain-containing protein
bn: Bacteriophage Mu GpT domain-containing protein
bo: Bacteriophage Mu GpT domain-containing protein
bp: Bacteriophage Mu GpT domain-containing protein
bq: Mu-like prophage FluMu N-terminal domain-containing protein
br: Mu-like prophage FluMu N-terminal domain-containing protein
bs: Mu-like prophage FluMu N-terminal domain-containing protein
bt: Mu-like prophage FluMu N-terminal domain-containing protein
bu: Mu-like prophage FluMu N-terminal domain-containing protein
bv: Mu-like prophage FluMu N-terminal domain-containing protein
bw: Mu-like prophage FluMu N-terminal domain-containing protein
bx: Bacteriophage Mu GpT domain-containing protein
by: Bacteriophage Mu GpT domain-containing protein
bz: Bacteriophage Mu GpT domain-containing protein
bA: Bacteriophage Mu GpT domain-containing protein
bB: Bacteriophage Mu GpT domain-containing protein
bC: Bacteriophage Mu GpT domain-containing protein
bD: Bacteriophage Mu GpT domain-containing protein
bE: Mu-like prophage FluMu N-terminal domain-containing protein
bF: Mu-like prophage FluMu N-terminal domain-containing protein
bG: Mu-like prophage FluMu N-terminal domain-containing protein
bH: Mu-like prophage FluMu N-terminal domain-containing protein
bI: Mu-like prophage FluMu N-terminal domain-containing protein
bJ: Mu-like prophage FluMu N-terminal domain-containing protein
bK: Mu-like prophage FluMu N-terminal domain-containing protein
bL: Bacteriophage Mu GpT domain-containing protein
bM: Bacteriophage Mu GpT domain-containing protein
bN: Bacteriophage Mu GpT domain-containing protein
bO: Bacteriophage Mu GpT domain-containing protein
bP: Bacteriophage Mu GpT domain-containing protein
bQ: Bacteriophage Mu GpT domain-containing protein
bR: Mu-like prophage FluMu N-terminal domain-containing protein
bS: Mu-like prophage FluMu N-terminal domain-containing protein
bT: Mu-like prophage FluMu N-terminal domain-containing protein
bU: Mu-like prophage FluMu N-terminal domain-containing protein
bV: Mu-like prophage FluMu N-terminal domain-containing protein
bW: Mu-like prophage FluMu N-terminal domain-containing protein
bX: Mu-like prophage FluMu N-terminal domain-containing protein
bY: Bacteriophage Mu GpT domain-containing protein
bZ: Bacteriophage Mu GpT domain-containing protein
ca: Bacteriophage Mu GpT domain-containing protein
cb: Bacteriophage Mu GpT domain-containing protein
cc: Bacteriophage Mu GpT domain-containing protein
cd: Bacteriophage Mu GpT domain-containing protein
ce: Bacteriophage Mu GpT domain-containing protein
cf: Mu-like prophage FluMu N-terminal domain-containing protein
cg: Mu-like prophage FluMu N-terminal domain-containing protein
ch: Mu-like prophage FluMu N-terminal domain-containing protein
ci: Mu-like prophage FluMu N-terminal domain-containing protein
cj: Mu-like prophage FluMu N-terminal domain-containing protein
ck: Mu-like prophage FluMu N-terminal domain-containing protein
cl: Mu-like prophage FluMu N-terminal domain-containing protein
cm: Bacteriophage Mu GpT domain-containing protein
cn: Bacteriophage Mu GpT domain-containing protein
co: Bacteriophage Mu GpT domain-containing protein
cp: Bacteriophage Mu GpT domain-containing protein
cq: Bacteriophage Mu GpT domain-containing protein
cr: Bacteriophage Mu GpT domain-containing protein
cs: Bacteriophage Mu GpT domain-containing protein
ct: Mu-like prophage FluMu N-terminal domain-containing protein
cu: Mu-like prophage FluMu N-terminal domain-containing protein
cv: Mu-like prophage FluMu N-terminal domain-containing protein
cw: Mu-like prophage FluMu N-terminal domain-containing protein
cx: Mu-like prophage FluMu N-terminal domain-containing protein
cy: Mu-like prophage FluMu N-terminal domain-containing protein
cz: Mu-like prophage FluMu N-terminal domain-containing protein
cA: Bacteriophage Mu GpT domain-containing protein
cB: Bacteriophage Mu GpT domain-containing protein
cC: Bacteriophage Mu GpT domain-containing protein
cD: Bacteriophage Mu GpT domain-containing protein
cE: Bacteriophage Mu GpT domain-containing protein
cF: Bacteriophage Mu GpT domain-containing protein
cG: Bacteriophage Mu GpT domain-containing protein
cH: Mu-like prophage FluMu N-terminal domain-containing protein
cI: Mu-like prophage FluMu N-terminal domain-containing protein
cJ: Mu-like prophage FluMu N-terminal domain-containing protein
cK: Mu-like prophage FluMu N-terminal domain-containing protein
cL: Mu-like prophage FluMu N-terminal domain-containing protein
cM: Mu-like prophage FluMu N-terminal domain-containing protein
cN: Mu-like prophage FluMu N-terminal domain-containing protein
cO: Bacteriophage Mu GpT domain-containing protein
cP: Bacteriophage Mu GpT domain-containing protein
cQ: Bacteriophage Mu GpT domain-containing protein
cR: Bacteriophage Mu GpT domain-containing protein
cS: Bacteriophage Mu GpT domain-containing protein
cT: Bacteriophage Mu GpT domain-containing protein
cU: Bacteriophage Mu GpT domain-containing protein
cV: Mu-like prophage FluMu N-terminal domain-containing protein
cW: Mu-like prophage FluMu N-terminal domain-containing protein
cX: Mu-like prophage FluMu N-terminal domain-containing protein
cY: Mu-like prophage FluMu N-terminal domain-containing protein
cZ: Mu-like prophage FluMu N-terminal domain-containing protein
da: Mu-like prophage FluMu N-terminal domain-containing protein
db: Mu-like prophage FluMu N-terminal domain-containing protein
dc: Bacteriophage Mu GpT domain-containing protein
dd: Bacteriophage Mu GpT domain-containing protein
de: Bacteriophage Mu GpT domain-containing protein
df: Bacteriophage Mu GpT domain-containing protein
dg: Bacteriophage Mu GpT domain-containing protein
dh: Bacteriophage Mu GpT domain-containing protein
di: Bacteriophage Mu GpT domain-containing protein
dj: Mu-like prophage FluMu N-terminal domain-containing protein
dk: Mu-like prophage FluMu N-terminal domain-containing protein
dl: Mu-like prophage FluMu N-terminal domain-containing protein
dm: Mu-like prophage FluMu N-terminal domain-containing protein
dn: Mu-like prophage FluMu N-terminal domain-containing protein
do: Mu-like prophage FluMu N-terminal domain-containing protein
dp: Mu-like prophage FluMu N-terminal domain-containing protein
dq: Bacteriophage Mu GpT domain-containing protein
dr: Bacteriophage Mu GpT domain-containing protein
ds: Bacteriophage Mu GpT domain-containing protein
dt: Bacteriophage Mu GpT domain-containing protein
du: Bacteriophage Mu GpT domain-containing protein
dv: Bacteriophage Mu GpT domain-containing protein
dw: Bacteriophage Mu GpT domain-containing protein
dx: Mu-like prophage FluMu N-terminal domain-containing protein
dy: Mu-like prophage FluMu N-terminal domain-containing protein
dz: Mu-like prophage FluMu N-terminal domain-containing protein
dA: Mu-like prophage FluMu N-terminal domain-containing protein
dB: Mu-like prophage FluMu N-terminal domain-containing protein
dC: Mu-like prophage FluMu N-terminal domain-containing protein
dD: Mu-like prophage FluMu N-terminal domain-containing protein
dE: Bacteriophage Mu GpT domain-containing protein
dF: Bacteriophage Mu GpT domain-containing protein
dG: Bacteriophage Mu GpT domain-containing protein
dH: Bacteriophage Mu GpT domain-containing protein
dI: Bacteriophage Mu GpT domain-containing protein
dJ: Bacteriophage Mu GpT domain-containing protein
dK: Bacteriophage Mu GpT domain-containing protein
dL: Mu-like prophage FluMu N-terminal domain-containing protein
dM: Mu-like prophage FluMu N-terminal domain-containing protein
dN: Mu-like prophage FluMu N-terminal domain-containing protein
dO: Mu-like prophage FluMu N-terminal domain-containing protein
dP: Mu-like prophage FluMu N-terminal domain-containing protein
dQ: Mu-like prophage FluMu N-terminal domain-containing protein
dR: Mu-like prophage FluMu N-terminal domain-containing protein
dS: Bacteriophage Mu GpT domain-containing protein
dT: Bacteriophage Mu GpT domain-containing protein
dU: Bacteriophage Mu GpT domain-containing protein
dV: Bacteriophage Mu GpT domain-containing protein
dW: Bacteriophage Mu GpT domain-containing protein
dX: Bacteriophage Mu GpT domain-containing protein
dY: Bacteriophage Mu GpT domain-containing protein
dZ: Mu-like prophage FluMu N-terminal domain-containing protein
ea: Mu-like prophage FluMu N-terminal domain-containing protein
eb: Mu-like prophage FluMu N-terminal domain-containing protein
ec: Mu-like prophage FluMu N-terminal domain-containing protein
ed: Mu-like prophage FluMu N-terminal domain-containing protein
ee: Mu-like prophage FluMu N-terminal domain-containing protein
ef: Mu-like prophage FluMu N-terminal domain-containing protein
eg: Bacteriophage Mu GpT domain-containing protein
eh: Bacteriophage Mu GpT domain-containing protein
ei: Bacteriophage Mu GpT domain-containing protein
ej: Bacteriophage Mu GpT domain-containing protein
ek: Bacteriophage Mu GpT domain-containing protein
el: Bacteriophage Mu GpT domain-containing protein
em: Bacteriophage Mu GpT domain-containing protein
en: Mu-like prophage FluMu N-terminal domain-containing protein
eo: Mu-like prophage FluMu N-terminal domain-containing protein
ep: Mu-like prophage FluMu N-terminal domain-containing protein
eq: Mu-like prophage FluMu N-terminal domain-containing protein
er: Mu-like prophage FluMu N-terminal domain-containing protein
es: Mu-like prophage FluMu N-terminal domain-containing protein
et: Mu-like prophage FluMu N-terminal domain-containing protein
eu: Bacteriophage Mu GpT domain-containing protein
ev: Bacteriophage Mu GpT domain-containing protein
ew: Bacteriophage Mu GpT domain-containing protein
ex: Bacteriophage Mu GpT domain-containing protein
ey: Bacteriophage Mu GpT domain-containing protein
ez: Bacteriophage Mu GpT domain-containing protein
eA: Bacteriophage Mu GpT domain-containing protein
eB: Mu-like prophage FluMu N-terminal domain-containing protein
eC: Mu-like prophage FluMu N-terminal domain-containing protein
eD: Mu-like prophage FluMu N-terminal domain-containing protein
eE: Mu-like prophage FluMu N-terminal domain-containing protein
eF: Mu-like prophage FluMu N-terminal domain-containing protein
eG: Mu-like prophage FluMu N-terminal domain-containing protein
eH: Mu-like prophage FluMu N-terminal domain-containing protein
eI: Bacteriophage Mu GpT domain-containing protein
eJ: Bacteriophage Mu GpT domain-containing protein
eK: Bacteriophage Mu GpT domain-containing protein
eL: Bacteriophage Mu GpT domain-containing protein
eM: Bacteriophage Mu GpT domain-containing protein
eN: Bacteriophage Mu GpT domain-containing protein
eO: Bacteriophage Mu GpT domain-containing protein
eP: Mu-like prophage FluMu N-terminal domain-containing protein
eQ: Mu-like prophage FluMu N-terminal domain-containing protein
eR: Mu-like prophage FluMu N-terminal domain-containing protein
eS: Mu-like prophage FluMu N-terminal domain-containing protein
eT: Mu-like prophage FluMu N-terminal domain-containing protein
eU: Mu-like prophage FluMu N-terminal domain-containing protein
eV: Mu-like prophage FluMu N-terminal domain-containing protein
eW: Bacteriophage Mu GpT domain-containing protein
eX: Bacteriophage Mu GpT domain-containing protein
eY: Bacteriophage Mu GpT domain-containing protein
eZ: Bacteriophage Mu GpT domain-containing protein
fa: Bacteriophage Mu GpT domain-containing protein
fb: Bacteriophage Mu GpT domain-containing protein
fc: Bacteriophage Mu GpT domain-containing protein
fd: Mu-like prophage FluMu N-terminal domain-containing protein
fe: Mu-like prophage FluMu N-terminal domain-containing protein
ff: Mu-like prophage FluMu N-terminal domain-containing protein
fg: Mu-like prophage FluMu N-terminal domain-containing protein
fh: Mu-like prophage FluMu N-terminal domain-containing protein
fi: Mu-like prophage FluMu N-terminal domain-containing protein
fj: Mu-like prophage FluMu N-terminal domain-containing protein
fk: Bacteriophage Mu GpT domain-containing protein
fl: Bacteriophage Mu GpT domain-containing protein
fm: Bacteriophage Mu GpT domain-containing protein
fn: Bacteriophage Mu GpT domain-containing protein
fo: Bacteriophage Mu GpT domain-containing protein
fp: Bacteriophage Mu GpT domain-containing protein
fq: Bacteriophage Mu GpT domain-containing protein
fr: Mu-like prophage FluMu N-terminal domain-containing protein
fs: Mu-like prophage FluMu N-terminal domain-containing protein
ft: Mu-like prophage FluMu N-terminal domain-containing protein
fu: Mu-like prophage FluMu N-terminal domain-containing protein
fv: Mu-like prophage FluMu N-terminal domain-containing protein
fw: Mu-like prophage FluMu N-terminal domain-containing protein
fx: Mu-like prophage FluMu N-terminal domain-containing protein
fy: Bacteriophage Mu GpT domain-containing protein
fz: Bacteriophage Mu GpT domain-containing protein
fA: Bacteriophage Mu GpT domain-containing protein
fB: Bacteriophage Mu GpT domain-containing protein
fC: Bacteriophage Mu GpT domain-containing protein
fD: Bacteriophage Mu GpT domain-containing protein
fE: Bacteriophage Mu GpT domain-containing protein
fF: Mu-like prophage FluMu N-terminal domain-containing protein
fG: Mu-like prophage FluMu N-terminal domain-containing protein
fH: Mu-like prophage FluMu N-terminal domain-containing protein
fI: Mu-like prophage FluMu N-terminal domain-containing protein
fJ: Mu-like prophage FluMu N-terminal domain-containing protein
fK: Mu-like prophage FluMu N-terminal domain-containing protein
fL: Mu-like prophage FluMu N-terminal domain-containing protein
fM: Bacteriophage Mu GpT domain-containing protein
fN: Bacteriophage Mu GpT domain-containing protein
fO: Bacteriophage Mu GpT domain-containing protein
fP: Bacteriophage Mu GpT domain-containing protein
fQ: Bacteriophage Mu GpT domain-containing protein
fR: Bacteriophage Mu GpT domain-containing protein
fS: Bacteriophage Mu GpT domain-containing protein
fT: Mu-like prophage FluMu N-terminal domain-containing protein
fU: Mu-like prophage FluMu N-terminal domain-containing protein
fV: Mu-like prophage FluMu N-terminal domain-containing protein
fW: Mu-like prophage FluMu N-terminal domain-containing protein
fX: Mu-like prophage FluMu N-terminal domain-containing protein
fY: Mu-like prophage FluMu N-terminal domain-containing protein
fZ: Mu-like prophage FluMu N-terminal domain-containing protein
ga: Bacteriophage Mu GpT domain-containing protein
gb: Bacteriophage Mu GpT domain-containing protein
gc: Bacteriophage Mu GpT domain-containing protein
gd: Bacteriophage Mu GpT domain-containing protein
ge: Bacteriophage Mu GpT domain-containing protein
gf: Bacteriophage Mu GpT domain-containing protein
gg: Bacteriophage Mu GpT domain-containing protein
gh: Mu-like prophage FluMu N-terminal domain-containing protein
gi: Mu-like prophage FluMu N-terminal domain-containing protein
gj: Mu-like prophage FluMu N-terminal domain-containing protein
gk: Mu-like prophage FluMu N-terminal domain-containing protein
gl: Mu-like prophage FluMu N-terminal domain-containing protein
gm: Mu-like prophage FluMu N-terminal domain-containing protein
gn: Mu-like prophage FluMu N-terminal domain-containing protein
go: Bacteriophage Mu GpT domain-containing protein
gp: Bacteriophage Mu GpT domain-containing protein
gq: Bacteriophage Mu GpT domain-containing protein
gr: Bacteriophage Mu GpT domain-containing protein
gs: Bacteriophage Mu GpT domain-containing protein
gt: Bacteriophage Mu GpT domain-containing protein
gu: Bacteriophage Mu GpT domain-containing protein
gv: Mu-like prophage FluMu N-terminal domain-containing protein
gw: Mu-like prophage FluMu N-terminal domain-containing protein
gx: Mu-like prophage FluMu N-terminal domain-containing protein
gy: Mu-like prophage FluMu N-terminal domain-containing protein
gz: Mu-like prophage FluMu N-terminal domain-containing protein
gA: Mu-like prophage FluMu N-terminal domain-containing protein
gB: Mu-like prophage FluMu N-terminal domain-containing protein
gC: Bacteriophage Mu GpT domain-containing protein
gD: Bacteriophage Mu GpT domain-containing protein
gE: Bacteriophage Mu GpT domain-containing protein
gF: Bacteriophage Mu GpT domain-containing protein
gG: Bacteriophage Mu GpT domain-containing protein
gH: Bacteriophage Mu GpT domain-containing protein
gI: Bacteriophage Mu GpT domain-containing protein
gJ: Mu-like prophage FluMu N-terminal domain-containing protein
gK: Mu-like prophage FluMu N-terminal domain-containing protein
gL: Mu-like prophage FluMu N-terminal domain-containing protein
gM: Mu-like prophage FluMu N-terminal domain-containing protein
gN: Mu-like prophage FluMu N-terminal domain-containing protein
gO: Mu-like prophage FluMu N-terminal domain-containing protein
gP: Mu-like prophage FluMu N-terminal domain-containing protein
gQ: Bacteriophage Mu GpT domain-containing protein
gR: Bacteriophage Mu GpT domain-containing protein
gS: Bacteriophage Mu GpT domain-containing protein
gT: Bacteriophage Mu GpT domain-containing protein
gU: Bacteriophage Mu GpT domain-containing protein
gV: Bacteriophage Mu GpT domain-containing protein
gW: Bacteriophage Mu GpT domain-containing protein
gX: Mu-like prophage FluMu N-terminal domain-containing protein
gY: Mu-like prophage FluMu N-terminal domain-containing protein
gZ: Mu-like prophage FluMu N-terminal domain-containing protein
ha: Mu-like prophage FluMu N-terminal domain-containing protein
hb: Mu-like prophage FluMu N-terminal domain-containing protein
hc: Mu-like prophage FluMu N-terminal domain-containing protein
hd: Mu-like prophage FluMu N-terminal domain-containing protein
he: Bacteriophage Mu GpT domain-containing protein
hf: Bacteriophage Mu GpT domain-containing protein
hg: Bacteriophage Mu GpT domain-containing protein
hh: Bacteriophage Mu GpT domain-containing protein
hi: Bacteriophage Mu GpT domain-containing protein
hj: Bacteriophage Mu GpT domain-containing protein
hk: Bacteriophage Mu GpT domain-containing protein
hl: Mu-like prophage FluMu N-terminal domain-containing protein
hm: Mu-like prophage FluMu N-terminal domain-containing protein
hn: Mu-like prophage FluMu N-terminal domain-containing protein
ho: Mu-like prophage FluMu N-terminal domain-containing protein
hp: Mu-like prophage FluMu N-terminal domain-containing protein
hq: Mu-like prophage FluMu N-terminal domain-containing protein
hr: Mu-like prophage FluMu N-terminal domain-containing protein
hs: Bacteriophage Mu GpT domain-containing protein
ht: Bacteriophage Mu GpT domain-containing protein
hu: Bacteriophage Mu GpT domain-containing protein
hv: Bacteriophage Mu GpT domain-containing protein
hw: Bacteriophage Mu GpT domain-containing protein
hx: Bacteriophage Mu GpT domain-containing protein
hy: Mu-like prophage FluMu N-terminal domain-containing protein
hz: Mu-like prophage FluMu N-terminal domain-containing protein
hA: Mu-like prophage FluMu N-terminal domain-containing protein
hB: Mu-like prophage FluMu N-terminal domain-containing protein
hC: Mu-like prophage FluMu N-terminal domain-containing protein
hD: Mu-like prophage FluMu N-terminal domain-containing protein
hE: Mu-like prophage FluMu N-terminal domain-containing protein
hF: Bacteriophage Mu GpT domain-containing protein
hG: Bacteriophage Mu GpT domain-containing protein
hH: Bacteriophage Mu GpT domain-containing protein
hI: Bacteriophage Mu GpT domain-containing protein
hJ: Bacteriophage Mu GpT domain-containing protein
hK: Bacteriophage Mu GpT domain-containing protein
hL: Mu-like prophage FluMu N-terminal domain-containing protein
hM: Mu-like prophage FluMu N-terminal domain-containing protein
hN: Mu-like prophage FluMu N-terminal domain-containing protein
hO: Mu-like prophage FluMu N-terminal domain-containing protein
hP: Mu-like prophage FluMu N-terminal domain-containing protein
hQ: Mu-like prophage FluMu N-terminal domain-containing protein
hR: Mu-like prophage FluMu N-terminal domain-containing protein
hS: Bacteriophage Mu GpT domain-containing protein
hT: Bacteriophage Mu GpT domain-containing protein
hU: Bacteriophage Mu GpT domain-containing protein
hV: Bacteriophage Mu GpT domain-containing protein
hW: Bacteriophage Mu GpT domain-containing protein
hX: Bacteriophage Mu GpT domain-containing protein
hY: Bacteriophage Mu GpT domain-containing protein
hZ: Mu-like prophage FluMu N-terminal domain-containing protein
ia: Mu-like prophage FluMu N-terminal domain-containing protein
ib: Mu-like prophage FluMu N-terminal domain-containing protein
ic: Mu-like prophage FluMu N-terminal domain-containing protein
id: Mu-like prophage FluMu N-terminal domain-containing protein
ie: Mu-like prophage FluMu N-terminal domain-containing protein
if: Mu-like prophage FluMu N-terminal domain-containing protein
ig: Bacteriophage Mu GpT domain-containing protein
ih: Bacteriophage Mu GpT domain-containing protein
ii: Bacteriophage Mu GpT domain-containing protein
ij: Bacteriophage Mu GpT domain-containing protein
ik: Bacteriophage Mu GpT domain-containing protein
il: Bacteriophage Mu GpT domain-containing protein
im: Bacteriophage Mu GpT domain-containing protein
in: Mu-like prophage FluMu N-terminal domain-containing protein
io: Mu-like prophage FluMu N-terminal domain-containing protein
ip: Mu-like prophage FluMu N-terminal domain-containing protein
iq: Mu-like prophage FluMu N-terminal domain-containing protein
ir: Mu-like prophage FluMu N-terminal domain-containing protein
is: Mu-like prophage FluMu N-terminal domain-containing protein
it: Mu-like prophage FluMu N-terminal domain-containing protein
iu: Bacteriophage Mu GpT domain-containing protein
iv: Bacteriophage Mu GpT domain-containing protein
iw: Bacteriophage Mu GpT domain-containing protein
ix: Bacteriophage Mu GpT domain-containing protein
iy: Bacteriophage Mu GpT domain-containing protein
iz: Bacteriophage Mu GpT domain-containing protein
iA: Bacteriophage Mu GpT domain-containing protein
iB: Mu-like prophage FluMu N-terminal domain-containing protein
iC: Mu-like prophage FluMu N-terminal domain-containing protein
iD: Mu-like prophage FluMu N-terminal domain-containing protein
iE: Mu-like prophage FluMu N-terminal domain-containing protein
iF: Mu-like prophage FluMu N-terminal domain-containing protein
iG: Mu-like prophage FluMu N-terminal domain-containing protein
iH: Mu-like prophage FluMu N-terminal domain-containing protein
iI: Bacteriophage Mu GpT domain-containing protein
iJ: Bacteriophage Mu GpT domain-containing protein
iK: Bacteriophage Mu GpT domain-containing protein
iL: Bacteriophage Mu GpT domain-containing protein
iM: Bacteriophage Mu GpT domain-containing protein
iN: Bacteriophage Mu GpT domain-containing protein
iO: Bacteriophage Mu GpT domain-containing protein
iP: Mu-like prophage FluMu N-terminal domain-containing protein
iQ: Mu-like prophage FluMu N-terminal domain-containing protein
iR: Mu-like prophage FluMu N-terminal domain-containing protein
iS: Mu-like prophage FluMu N-terminal domain-containing protein
iT: Mu-like prophage FluMu N-terminal domain-containing protein
iU: Mu-like prophage FluMu N-terminal domain-containing protein
iV: Mu-like prophage FluMu N-terminal domain-containing protein
iW: Bacteriophage Mu GpT domain-containing protein
iX: Bacteriophage Mu GpT domain-containing protein
iY: Bacteriophage Mu GpT domain-containing protein
iZ: Bacteriophage Mu GpT domain-containing protein
ja: Bacteriophage Mu GpT domain-containing protein
jb: Bacteriophage Mu GpT domain-containing protein
jc: Bacteriophage Mu GpT domain-containing protein
jd: Mu-like prophage FluMu N-terminal domain-containing protein
je: Mu-like prophage FluMu N-terminal domain-containing protein
jf: Mu-like prophage FluMu N-terminal domain-containing protein
jg: Mu-like prophage FluMu N-terminal domain-containing protein
jh: Mu-like prophage FluMu N-terminal domain-containing protein
ji: Mu-like prophage FluMu N-terminal domain-containing protein
jj: Mu-like prophage FluMu N-terminal domain-containing protein
jk: Bacteriophage Mu GpT domain-containing protein
jl: Bacteriophage Mu GpT domain-containing protein
jm: Bacteriophage Mu GpT domain-containing protein
jn: Bacteriophage Mu GpT domain-containing protein
jo: Bacteriophage Mu GpT domain-containing protein
jp: Bacteriophage Mu GpT domain-containing protein
jq: Bacteriophage Mu GpT domain-containing protein
jr: Mu-like prophage FluMu N-terminal domain-containing protein
js: Mu-like prophage FluMu N-terminal domain-containing protein
jt: Mu-like prophage FluMu N-terminal domain-containing protein
ju: Mu-like prophage FluMu N-terminal domain-containing protein
jv: Mu-like prophage FluMu N-terminal domain-containing protein
jw: Mu-like prophage FluMu N-terminal domain-containing protein
jx: Mu-like prophage FluMu N-terminal domain-containing protein
jy: Bacteriophage Mu GpT domain-containing protein
jz: Bacteriophage Mu GpT domain-containing protein
jA: Bacteriophage Mu GpT domain-containing protein
jB: Bacteriophage Mu GpT domain-containing protein
jC: Bacteriophage Mu GpT domain-containing protein
jD: Bacteriophage Mu GpT domain-containing protein
jE: Bacteriophage Mu GpT domain-containing protein
jF: Mu-like prophage FluMu N-terminal domain-containing protein
jG: Mu-like prophage FluMu N-terminal domain-containing protein
jH: Mu-like prophage FluMu N-terminal domain-containing protein
jI: Mu-like prophage FluMu N-terminal domain-containing protein
jJ: Mu-like prophage FluMu N-terminal domain-containing protein
jK: Mu-like prophage FluMu N-terminal domain-containing protein
jL: Mu-like prophage FluMu N-terminal domain-containing protein
jM: Bacteriophage Mu GpT domain-containing protein
jN: Bacteriophage Mu GpT domain-containing protein
jO: Bacteriophage Mu GpT domain-containing protein
jP: Bacteriophage Mu GpT domain-containing protein
jQ: Bacteriophage Mu GpT domain-containing protein
jR: Bacteriophage Mu GpT domain-containing protein
jS: Bacteriophage Mu GpT domain-containing protein
jT: Mu-like prophage FluMu N-terminal domain-containing protein
jU: Mu-like prophage FluMu N-terminal domain-containing protein
jV: Mu-like prophage FluMu N-terminal domain-containing protein
jW: Mu-like prophage FluMu N-terminal domain-containing protein
jX: Mu-like prophage FluMu N-terminal domain-containing protein
jY: Mu-like prophage FluMu N-terminal domain-containing protein
jZ: Mu-like prophage FluMu N-terminal domain-containing protein
ka: Bacteriophage Mu GpT domain-containing protein
kb: Bacteriophage Mu GpT domain-containing protein
kc: Bacteriophage Mu GpT domain-containing protein
kd: Bacteriophage Mu GpT domain-containing protein
ke: Bacteriophage Mu GpT domain-containing protein
kf: Bacteriophage Mu GpT domain-containing protein
kg: Bacteriophage Mu GpT domain-containing protein
kh: Mu-like prophage FluMu N-terminal domain-containing protein
ki: Mu-like prophage FluMu N-terminal domain-containing protein
kj: Mu-like prophage FluMu N-terminal domain-containing protein
kk: Mu-like prophage FluMu N-terminal domain-containing protein
kl: Mu-like prophage FluMu N-terminal domain-containing protein
km: Mu-like prophage FluMu N-terminal domain-containing protein
kn: Mu-like prophage FluMu N-terminal domain-containing protein
ko: Bacteriophage Mu GpT domain-containing protein
kp: Bacteriophage Mu GpT domain-containing protein
kq: Bacteriophage Mu GpT domain-containing protein
kr: Bacteriophage Mu GpT domain-containing protein
ks: Bacteriophage Mu GpT domain-containing protein
kt: Bacteriophage Mu GpT domain-containing protein
ku: Mu-like prophage FluMu N-terminal domain-containing protein
kv: Mu-like prophage FluMu N-terminal domain-containing protein
kw: Mu-like prophage FluMu N-terminal domain-containing protein
kx: Mu-like prophage FluMu N-terminal domain-containing protein
ky: Mu-like prophage FluMu N-terminal domain-containing protein
kz: Mu-like prophage FluMu N-terminal domain-containing protein
kA: Mu-like prophage FluMu N-terminal domain-containing protein
kB: Bacteriophage Mu GpT domain-containing protein
kC: Bacteriophage Mu GpT domain-containing protein
kD: Bacteriophage Mu GpT domain-containing protein
kE: Bacteriophage Mu GpT domain-containing protein
kF: Bacteriophage Mu GpT domain-containing protein
kG: Bacteriophage Mu GpT domain-containing protein
kH: Bacteriophage Mu GpT domain-containing protein
kI: Mu-like prophage FluMu N-terminal domain-containing protein
kJ: Mu-like prophage FluMu N-terminal domain-containing protein
kK: Mu-like prophage FluMu N-terminal domain-containing protein
kL: Mu-like prophage FluMu N-terminal domain-containing protein
kM: Mu-like prophage FluMu N-terminal domain-containing protein
kN: Mu-like prophage FluMu N-terminal domain-containing protein
kO: Mu-like prophage FluMu N-terminal domain-containing protein
kP: Bacteriophage Mu GpT domain-containing protein
kQ: Bacteriophage Mu GpT domain-containing protein
kR: Bacteriophage Mu GpT domain-containing protein
kS: Bacteriophage Mu GpT domain-containing protein
kT: Bacteriophage Mu GpT domain-containing protein
kU: Bacteriophage Mu GpT domain-containing protein
kV: Bacteriophage Mu GpT domain-containing protein
kW: Mu-like prophage FluMu N-terminal domain-containing protein
kX: Mu-like prophage FluMu N-terminal domain-containing protein
kY: Mu-like prophage FluMu N-terminal domain-containing protein
kZ: Mu-like prophage FluMu N-terminal domain-containing protein
la: Mu-like prophage FluMu N-terminal domain-containing protein
lb: Mu-like prophage FluMu N-terminal domain-containing protein
lc: Mu-like prophage FluMu N-terminal domain-containing protein
ld: Bacteriophage Mu GpT domain-containing protein
le: Bacteriophage Mu GpT domain-containing protein
lf: Bacteriophage Mu GpT domain-containing protein
lg: Bacteriophage Mu GpT domain-containing protein
lh: Bacteriophage Mu GpT domain-containing protein
li: Bacteriophage Mu GpT domain-containing protein
lj: Bacteriophage Mu GpT domain-containing protein
lk: Mu-like prophage FluMu N-terminal domain-containing protein
ll: Mu-like prophage FluMu N-terminal domain-containing protein
lm: Mu-like prophage FluMu N-terminal domain-containing protein
ln: Mu-like prophage FluMu N-terminal domain-containing protein
lo: Mu-like prophage FluMu N-terminal domain-containing protein
lp: Mu-like prophage FluMu N-terminal domain-containing protein
lq: Mu-like prophage FluMu N-terminal domain-containing protein
lr: Bacteriophage Mu GpT domain-containing protein
ls: Bacteriophage Mu GpT domain-containing protein
lt: Bacteriophage Mu GpT domain-containing protein
lu: Bacteriophage Mu GpT domain-containing protein
lv: Bacteriophage Mu GpT domain-containing protein
lw: Bacteriophage Mu GpT domain-containing protein
lx: Bacteriophage Mu GpT domain-containing protein
ly: Mu-like prophage FluMu N-terminal domain-containing protein
lz: Mu-like prophage FluMu N-terminal domain-containing protein
lA: Mu-like prophage FluMu N-terminal domain-containing protein
lB: Mu-like prophage FluMu N-terminal domain-containing protein
lC: Mu-like prophage FluMu N-terminal domain-containing protein
lD: Mu-like prophage FluMu N-terminal domain-containing protein
lE: Mu-like prophage FluMu N-terminal domain-containing protein
lF: Bacteriophage Mu GpT domain-containing protein
lG: Bacteriophage Mu GpT domain-containing protein
lH: Bacteriophage Mu GpT domain-containing protein
lI: Bacteriophage Mu GpT domain-containing protein
lJ: Bacteriophage Mu GpT domain-containing protein
lK: Bacteriophage Mu GpT domain-containing protein
lL: Bacteriophage Mu GpT domain-containing protein
lM: Mu-like prophage FluMu N-terminal domain-containing protein
lN: Mu-like prophage FluMu N-terminal domain-containing protein
lO: Mu-like prophage FluMu N-terminal domain-containing protein
lP: Mu-like prophage FluMu N-terminal domain-containing protein
lQ: Mu-like prophage FluMu N-terminal domain-containing protein
lR: Mu-like prophage FluMu N-terminal domain-containing protein
lS: Mu-like prophage FluMu N-terminal domain-containing protein
lT: Bacteriophage Mu GpT domain-containing protein
lU: Bacteriophage Mu GpT domain-containing protein
lV: Bacteriophage Mu GpT domain-containing protein
lW: Bacteriophage Mu GpT domain-containing protein
lX: Bacteriophage Mu GpT domain-containing protein
lY: Bacteriophage Mu GpT domain-containing protein
lZ: Bacteriophage Mu GpT domain-containing protein
ma: Mu-like prophage FluMu N-terminal domain-containing protein
mb: Mu-like prophage FluMu N-terminal domain-containing protein
mc: Mu-like prophage FluMu N-terminal domain-containing protein
md: Mu-like prophage FluMu N-terminal domain-containing protein
me: Mu-like prophage FluMu N-terminal domain-containing protein
mf: Mu-like prophage FluMu N-terminal domain-containing protein
mg: Mu-like prophage FluMu N-terminal domain-containing protein
mh: Bacteriophage Mu GpT domain-containing protein
mi: Bacteriophage Mu GpT domain-containing protein
mj: Bacteriophage Mu GpT domain-containing protein
mk: Bacteriophage Mu GpT domain-containing protein
ml: Bacteriophage Mu GpT domain-containing protein
mm: Bacteriophage Mu GpT domain-containing protein
mn: Bacteriophage Mu GpT domain-containing protein
mo: Mu-like prophage FluMu N-terminal domain-containing protein
mp: Mu-like prophage FluMu N-terminal domain-containing protein
mq: Mu-like prophage FluMu N-terminal domain-containing protein
mr: Mu-like prophage FluMu N-terminal domain-containing protein
ms: Mu-like prophage FluMu N-terminal domain-containing protein
mt: Mu-like prophage FluMu N-terminal domain-containing protein
mu: Mu-like prophage FluMu N-terminal domain-containing protein
mv: Bacteriophage Mu GpT domain-containing protein
mw: Bacteriophage Mu GpT domain-containing protein
mx: Bacteriophage Mu GpT domain-containing protein
my: Bacteriophage Mu GpT domain-containing protein
mz: Bacteriophage Mu GpT domain-containing protein
mA: Bacteriophage Mu GpT domain-containing protein
mB: Bacteriophage Mu GpT domain-containing protein
mC: Mu-like prophage FluMu N-terminal domain-containing protein
mD: Mu-like prophage FluMu N-terminal domain-containing protein
mE: Mu-like prophage FluMu N-terminal domain-containing protein
mF: Mu-like prophage FluMu N-terminal domain-containing protein
mG: Mu-like prophage FluMu N-terminal domain-containing protein
mH: Mu-like prophage FluMu N-terminal domain-containing protein
mI: Mu-like prophage FluMu N-terminal domain-containing protein
mJ: Bacteriophage Mu GpT domain-containing protein
mK: Bacteriophage Mu GpT domain-containing protein
mL: Bacteriophage Mu GpT domain-containing protein
mM: Bacteriophage Mu GpT domain-containing protein
mN: Bacteriophage Mu GpT domain-containing protein
mO: Bacteriophage Mu GpT domain-containing protein
mP: Bacteriophage Mu GpT domain-containing protein
mQ: Mu-like prophage FluMu N-terminal domain-containing protein
mR: Mu-like prophage FluMu N-terminal domain-containing protein
mS: Mu-like prophage FluMu N-terminal domain-containing protein
mT: Mu-like prophage FluMu N-terminal domain-containing protein
mU: Mu-like prophage FluMu N-terminal domain-containing protein
mV: Mu-like prophage FluMu N-terminal domain-containing protein
mW: Mu-like prophage FluMu N-terminal domain-containing protein
mX: Bacteriophage Mu GpT domain-containing protein
mY: Bacteriophage Mu GpT domain-containing protein
mZ: Bacteriophage Mu GpT domain-containing protein
na: Bacteriophage Mu GpT domain-containing protein
nb: Bacteriophage Mu GpT domain-containing protein
nc: Bacteriophage Mu GpT domain-containing protein
nd: Bacteriophage Mu GpT domain-containing protein
ne: Mu-like prophage FluMu N-terminal domain-containing protein
nf: Mu-like prophage FluMu N-terminal domain-containing protein
ng: Mu-like prophage FluMu N-terminal domain-containing protein
nh: Mu-like prophage FluMu N-terminal domain-containing protein
ni: Mu-like prophage FluMu N-terminal domain-containing protein
nj: Mu-like prophage FluMu N-terminal domain-containing protein
nk: Mu-like prophage FluMu N-terminal domain-containing protein
nl: Bacteriophage Mu GpT domain-containing protein
nm: Bacteriophage Mu GpT domain-containing protein
nn: Bacteriophage Mu GpT domain-containing protein
no: Bacteriophage Mu GpT domain-containing protein
np: Bacteriophage Mu GpT domain-containing protein
nq: Bacteriophage Mu GpT domain-containing protein
nr: Bacteriophage Mu GpT domain-containing protein
ns: Mu-like prophage FluMu N-terminal domain-containing protein
nt: Mu-like prophage FluMu N-terminal domain-containing protein
nu: Mu-like prophage FluMu N-terminal domain-containing protein
nv: Mu-like prophage FluMu N-terminal domain-containing protein
nw: Mu-like prophage FluMu N-terminal domain-containing protein
nx: Mu-like prophage FluMu N-terminal domain-containing protein
ny: Mu-like prophage FluMu N-terminal domain-containing protein
nz: Bacteriophage Mu GpT domain-containing protein
nA: Bacteriophage Mu GpT domain-containing protein
nB: Bacteriophage Mu GpT domain-containing protein
nC: Bacteriophage Mu GpT domain-containing protein
nD: Bacteriophage Mu GpT domain-containing protein
nE: Bacteriophage Mu GpT domain-containing protein
nF: Bacteriophage Mu GpT domain-containing protein
nG: Mu-like prophage FluMu N-terminal domain-containing protein
nH: Mu-like prophage FluMu N-terminal domain-containing protein
nI: Mu-like prophage FluMu N-terminal domain-containing protein
nJ: Mu-like prophage FluMu N-terminal domain-containing protein
nK: Mu-like prophage FluMu N-terminal domain-containing protein
nL: Mu-like prophage FluMu N-terminal domain-containing protein
nM: Mu-like prophage FluMu N-terminal domain-containing protein
nN: Bacteriophage Mu GpT domain-containing protein
nO: Bacteriophage Mu GpT domain-containing protein
nP: Bacteriophage Mu GpT domain-containing protein
nQ: Bacteriophage Mu GpT domain-containing protein
nR: Bacteriophage Mu GpT domain-containing protein
nS: Bacteriophage Mu GpT domain-containing protein
nT: Bacteriophage Mu GpT domain-containing protein
nU: Mu-like prophage FluMu N-terminal domain-containing protein
nV: Mu-like prophage FluMu N-terminal domain-containing protein
nW: Mu-like prophage FluMu N-terminal domain-containing protein
nX: Mu-like prophage FluMu N-terminal domain-containing protein
nY: Mu-like prophage FluMu N-terminal domain-containing protein
nZ: Mu-like prophage FluMu N-terminal domain-containing protein
oa: Mu-like prophage FluMu N-terminal domain-containing protein
ob: Bacteriophage Mu GpT domain-containing protein
oc: Bacteriophage Mu GpT domain-containing protein
od: Bacteriophage Mu GpT domain-containing protein
oe: Bacteriophage Mu GpT domain-containing protein
of: Bacteriophage Mu GpT domain-containing protein
og: Bacteriophage Mu GpT domain-containing protein
oh: Bacteriophage Mu GpT domain-containing protein
oi: Mu-like prophage FluMu N-terminal domain-containing protein
oj: Mu-like prophage FluMu N-terminal domain-containing protein
ok: Mu-like prophage FluMu N-terminal domain-containing protein
ol: Mu-like prophage FluMu N-terminal domain-containing protein
om: Mu-like prophage FluMu N-terminal domain-containing protein
on: Mu-like prophage FluMu N-terminal domain-containing protein
oo: Mu-like prophage FluMu N-terminal domain-containing protein
op: Bacteriophage Mu GpT domain-containing protein
oq: Bacteriophage Mu GpT domain-containing protein
or: Bacteriophage Mu GpT domain-containing protein
os: Bacteriophage Mu GpT domain-containing protein
ot: Bacteriophage Mu GpT domain-containing protein
ou: Bacteriophage Mu GpT domain-containing protein
ov: Bacteriophage Mu GpT domain-containing protein
ow: Mu-like prophage FluMu N-terminal domain-containing protein
ox: Mu-like prophage FluMu N-terminal domain-containing protein
oy: Mu-like prophage FluMu N-terminal domain-containing protein
oz: Mu-like prophage FluMu N-terminal domain-containing protein
oA: Mu-like prophage FluMu N-terminal domain-containing protein
oB: Mu-like prophage FluMu N-terminal domain-containing protein
oC: Mu-like prophage FluMu N-terminal domain-containing protein
oD: Bacteriophage Mu GpT domain-containing protein
oE: Bacteriophage Mu GpT domain-containing protein
oF: Bacteriophage Mu GpT domain-containing protein
oG: Bacteriophage Mu GpT domain-containing protein
oH: Bacteriophage Mu GpT domain-containing protein
oI: Bacteriophage Mu GpT domain-containing protein
oJ: Bacteriophage Mu GpT domain-containing protein
oK: Mu-like prophage FluMu N-terminal domain-containing protein
oL: Mu-like prophage FluMu N-terminal domain-containing protein
oM: Mu-like prophage FluMu N-terminal domain-containing protein
oN: Mu-like prophage FluMu N-terminal domain-containing protein
oO: Mu-like prophage FluMu N-terminal domain-containing protein
oP: Mu-like prophage FluMu N-terminal domain-containing protein
oQ: Mu-like prophage FluMu N-terminal domain-containing protein
oR: Bacteriophage Mu GpT domain-containing protein
oS: Bacteriophage Mu GpT domain-containing protein
oT: Bacteriophage Mu GpT domain-containing protein
oU: Bacteriophage Mu GpT domain-containing protein
oV: Bacteriophage Mu GpT domain-containing protein
oW: Bacteriophage Mu GpT domain-containing protein
oX: Bacteriophage Mu GpT domain-containing protein
oY: Mu-like prophage FluMu N-terminal domain-containing protein
oZ: Mu-like prophage FluMu N-terminal domain-containing protein
pa: Mu-like prophage FluMu N-terminal domain-containing protein
pb: Mu-like prophage FluMu N-terminal domain-containing protein
pc: Mu-like prophage FluMu N-terminal domain-containing protein
pd: Mu-like prophage FluMu N-terminal domain-containing protein
pe: Mu-like prophage FluMu N-terminal domain-containing protein
pf: Bacteriophage Mu GpT domain-containing protein
pg: Bacteriophage Mu GpT domain-containing protein
ph: Bacteriophage Mu GpT domain-containing protein
pi: Bacteriophage Mu GpT domain-containing protein
pj: Bacteriophage Mu GpT domain-containing protein
pk: Bacteriophage Mu GpT domain-containing protein
pl: Bacteriophage Mu GpT domain-containing protein
pm: Mu-like prophage FluMu N-terminal domain-containing protein
pn: Mu-like prophage FluMu N-terminal domain-containing protein
po: Mu-like prophage FluMu N-terminal domain-containing protein
pp: Mu-like prophage FluMu N-terminal domain-containing protein
pq: Mu-like prophage FluMu N-terminal domain-containing protein
pr: Mu-like prophage FluMu N-terminal domain-containing protein
ps: Mu-like prophage FluMu N-terminal domain-containing protein
pt: Bacteriophage Mu GpT domain-containing protein
pu: Bacteriophage Mu GpT domain-containing protein
pv: Bacteriophage Mu GpT domain-containing protein
pw: Bacteriophage Mu GpT domain-containing protein
px: Bacteriophage Mu GpT domain-containing protein
py: Bacteriophage Mu GpT domain-containing protein
pz: Bacteriophage Mu GpT domain-containing protein
pA: Mu-like prophage FluMu N-terminal domain-containing protein
pB: Mu-like prophage FluMu N-terminal domain-containing protein
pC: Mu-like prophage FluMu N-terminal domain-containing protein
pD: Mu-like prophage FluMu N-terminal domain-containing protein
pE: Mu-like prophage FluMu N-terminal domain-containing protein
pF: Mu-like prophage FluMu N-terminal domain-containing protein
pG: Mu-like prophage FluMu N-terminal domain-containing protein
pH: Bacteriophage Mu GpT domain-containing protein
pI: Bacteriophage Mu GpT domain-containing protein
pJ: Bacteriophage Mu GpT domain-containing protein
pK: Bacteriophage Mu GpT domain-containing protein
pL: Bacteriophage Mu GpT domain-containing protein
pM: Bacteriophage Mu GpT domain-containing protein
pN: Bacteriophage Mu GpT domain-containing protein
pO: Mu-like prophage FluMu N-terminal domain-containing protein
pP: Mu-like prophage FluMu N-terminal domain-containing protein
pQ: Mu-like prophage FluMu N-terminal domain-containing protein
pR: Mu-like prophage FluMu N-terminal domain-containing protein
pS: Mu-like prophage FluMu N-terminal domain-containing protein
pT: Mu-like prophage FluMu N-terminal domain-containing protein
pU: Mu-like prophage FluMu N-terminal domain-containing protein
pV: Bacteriophage Mu GpT domain-containing protein
pW: Bacteriophage Mu GpT domain-containing protein
pX: Bacteriophage Mu GpT domain-containing protein
pY: Bacteriophage Mu GpT domain-containing protein
pZ: Bacteriophage Mu GpT domain-containing protein
qa: Bacteriophage Mu GpT domain-containing protein
qb: Bacteriophage Mu GpT domain-containing protein
qc: Mu-like prophage FluMu N-terminal domain-containing protein
qd: Stopper protein
qe: Stopper protein
qf: Stopper protein
qg: Stopper protein
qh: Stopper protein
qi: Stopper protein
qj: Virion structural protein
qk: Virion structural protein
ql: Virion structural protein
qm: Virion structural protein
qn: Virion structural protein
qo: Virion structural protein
qp: Portal protein
qq: DUF1320 domain-containing protein
qr: Portal protein
qs: DUF1320 domain-containing protein
qt: Portal protein
qu: DUF1320 domain-containing protein
qv: Portal protein
qw: DUF1320 domain-containing protein
qx: Portal protein
qy: DUF1320 domain-containing protein
qz: Portal protein
qA: DUF1320 domain-containing protein
qB: Portal protein
qC: DUF1320 domain-containing protein
qD: Portal protein
qE: DUF1320 domain-containing protein
qF: Portal protein
qG: DUF1320 domain-containing protein
qH: Portal protein
qI: DUF1320 domain-containing protein
qJ: Portal protein
qK: DUF1320 domain-containing protein
qL: Portal protein
qM: DUF1320 domain-containing protein


Theoretical massNumber of molelcules
Total (without water)20,209,706871
Polymers20,209,706871
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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Protein , 6 types, 871 molecules aaabacadaeafanaoapaqarasataAaBaCaDaEaFaGaOaPaQaRaSaTaUbcbdbe...

#1: Protein ...
Mu-like prophage FluMu N-terminal domain-containing protein


Mass: 12112.113 Da / Num. of mol.: 420 / Source method: isolated from a natural source / Details: Minor capsid protein gp39 / Source: (natural) Pseudomonas phage JBD30 (virus) / References: UniProt: L7P800
#2: Protein ...
Bacteriophage Mu GpT domain-containing protein


Mass: 33694.980 Da / Num. of mol.: 415 / Source method: isolated from a natural source / Details: major capsid protein gp38 / Source: (natural) Pseudomonas phage JBD30 (virus) / References: UniProt: L7P7W9
#3: Protein
Stopper protein


Mass: 16990.168 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Details: stopper protein gp42 / Source: (natural) Pseudomonas phage JBD30 (virus) / References: UniProt: L7P7R4
#4: Protein
Virion structural protein


Mass: 26983.729 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Details: major tail protein gp44 / Source: (natural) Pseudomonas phage JBD30 (virus) / References: UniProt: L7P801
#5: Protein
Portal protein


Mass: 57791.332 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Details: portal protein gp32 / Source: (natural) Pseudomonas phage JBD30 (virus) / References: UniProt: L7P7R0
#6: Protein
DUF1320 domain-containing protein


Mass: 15155.176 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Details: adaptor protein gp41 / Source: (natural) Pseudomonas phage JBD30 (virus) / References: UniProt: L7P846

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Details

Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Pseudomonas phage JBD30 / Type: VIRUS
Details: Phage JBD30 was propagated in P. aeruginosa strain BAA-28 and purified using CsCl gradient.
Entity ID: all / Source: NATURAL
Molecular weightValue: 20.19 MDa / Experimental value: NO
Source (natural)Organism: Pseudomonas phage JBD30 (virus)
Details of virusEmpty: NO / Enveloped: NO / Isolate: STRAIN / Type: VIRION
Natural hostOrganism: Pseudomonas aeruginosa / Strain: BAA-28
Virus shellName: JBD30 capsid / Diameter: 640 nm / Triangulation number (T number): 7
Buffer solutionpH: 8 / Details: 10 mM MgSO4, 10 mM NaCl, 50 mM Tris pH 8
Buffer component
IDConc.NameFormulaBuffer-ID
110 mMsodium chlorideNaCl1
210 mMmagnesium sulphateMgSO41
350 mMTris hydrochlorideTris-HCl1
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES / Details: phage titer 10^11 PFU
Specimen supportDetails: Gatan Solarus II / Grid material: COPPER / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R2/1
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277.15 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal magnification: 130000 X / Nominal defocus max: 1600 nm / Nominal defocus min: 600 nm / Cs: 2.7 mm / C2 aperture diameter: 50 µm / Alignment procedure: COMA FREE
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingAverage exposure time: 5 sec. / Electron dose: 30 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Num. of grids imaged: 1 / Num. of real images: 11300
EM imaging opticsEnergyfilter name: GIF Quantum LS / Energyfilter slit width: 20 eV
Image scansWidth: 3838 / Height: 3710 / Movie frames/image: 25 / Used frames/image: 1-25

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Processing

EM software
IDNameVersionCategoryDetails
1crYOLO1.7.6particle selection
2EPUimage acquisition
4Gctf1.06CTF correctionctf estimation
10RELION3.1initial Euler assignment
11RELION3.1final Euler assignment
12RELION3.1classification
13RELION3.13D reconstruction
CTF correctionType: PHASE FLIPPING ONLY
Particle selectionNum. of particles selected: 13129
SymmetryPoint symmetry: C1 (asymmetric)
3D reconstructionResolution: 8 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 2913 / Symmetry type: POINT

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