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- PDB-8rko: Bacteriophage JBD30 empty particle capsid -

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Basic information

Entry
Database: PDB / ID: 8rko
TitleBacteriophage JBD30 empty particle capsid
ComponentsBacteriophage Mu GpT domain-containing protein
KeywordsVIRUS / bacteriophage JBD30 / virion / capsid / empty particle
Function / homologyBacteriophage Mu, GpT / Mu-like prophage major head subunit gpT / Bacteriophage Mu GpT domain-containing protein
Function and homology information
Biological speciesPseudomonas phage JBD30 (virus)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.58 Å
AuthorsValentova, L. / Fuzik, T. / Plevka, P.
Funding support Czech Republic, European Union, 2items
OrganizationGrant numberCountry
Ministry of Education, Youth and Sports of the Czech RepublicLX22NPO5103 Czech Republic
European Research Council (ERC)101043452European Union
CitationJournal: Embo J. / Year: 2024
Title: Structure and replication of Pseudomonas aeruginosa phage JBD30
Authors: Valentova, L. / Plevka, P. / Fuzik, T. / Novacek, J. / Pospisil, J.
History
DepositionDec 27, 2023Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 14, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein


Theoretical massNumber of molelcules
Total (without water)235,8657
Polymers235,8657
Non-polymers00
Water00
1
C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
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Z: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
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B: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
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B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
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B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein

C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
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U: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
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Z: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
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C: Bacteriophage Mu GpT domain-containing protein
M: Bacteriophage Mu GpT domain-containing protein
L: Bacteriophage Mu GpT domain-containing protein
B: Bacteriophage Mu GpT domain-containing protein
Z: Bacteriophage Mu GpT domain-containing protein
U: Bacteriophage Mu GpT domain-containing protein
K: Bacteriophage Mu GpT domain-containing protein


Theoretical massNumber of molelcules
Total (without water)14,151,892420
Polymers14,151,892420
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
point symmetry operation59
MethodUCSF CHIMERA

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Components

#1: Protein
Bacteriophage Mu GpT domain-containing protein


Mass: 33694.980 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Pseudomonas phage JBD30 (virus) / References: UniProt: L7P7W9

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Pseudomonas phage JBD30 / Type: VIRUS
Details: Phage JBD30 was propagated in P. aeruginosa strain BAA-28 and purified using CsCl gradient.
Entity ID: all / Source: NATURAL
Molecular weightValue: 13.971 MDa / Experimental value: NO
Source (natural)Organism: Pseudomonas phage JBD30 (virus)
Details of virusEmpty: NO / Enveloped: NO / Isolate: STRAIN / Type: VIRION
Natural hostOrganism: Pseudomonas aeruginosa / Strain: BAA-28
Virus shellName: JBD30 capsid / Diameter: 640 nm / Triangulation number (T number): 7
Buffer solutionpH: 8 / Details: 10 mM MgSO4, 10 mM NaCl, 50 mM Tris pH 8
Buffer component
IDConc.NameFormulaBuffer-ID
110 mMsodium chlorideNaCl1
210 mMmagnesium sulphateMgSO41
350 mMTris hydrochlorideTris-HCl1
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES / Details: phage titer 10^11 PFU
Specimen supportDetails: Gatan Solaris II / Grid material: COPPER / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R2/1
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277.15 K
Details: blotting force 0, blotting time 2 s, waiting time 15 s

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal magnification: 105000 X / Nominal defocus max: 1600 nm / Nominal defocus min: 600 nm / Cs: 2.7 mm / C2 aperture diameter: 50 µm / Alignment procedure: COMA FREE
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingAverage exposure time: 2 sec. / Electron dose: 34 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) / Num. of grids imaged: 1 / Num. of real images: 12356
EM imaging opticsEnergyfilter name: GIF Bioquantum / Energyfilter slit width: 10 eV
Image scansWidth: 5760 / Height: 4092

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Processing

EM software
IDNameVersionCategoryDetails
1crYOLO1.8particle selection
2EPUimage acquisition
4Gctf1.06CTF correctionctf estimation
7Coot0.9.8.7model fitting
9RELION3.1initial Euler assignment
10RELION3.1final Euler assignment
11RELION3.1classification
12RELION3.13D reconstruction
13PHENIX1.20.1model refinement
CTF correctionType: PHASE FLIPPING ONLY
Particle selectionNum. of particles selected: 3037
SymmetryPoint symmetry: I (icosahedral)
3D reconstructionResolution: 2.58 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 2666 / Algorithm: FOURIER SPACE / Num. of class averages: 1 / Symmetry type: POINT
Atomic model buildingProtocol: OTHER / Space: REAL
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.0160300
ELECTRON MICROSCOPYf_angle_d0.87882050
ELECTRON MICROSCOPYf_dihedral_angle_d4.9518175
ELECTRON MICROSCOPYf_chiral_restr0.0429125
ELECTRON MICROSCOPYf_plane_restr0.00610800

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