+Open data
-Basic information
Entry | Database: PDB / ID: 8rkx | |||||||||
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Title | Procapsid of bacteriophage JBD30 computed in I4 symmetry | |||||||||
Components | Bacteriophage Mu GpT domain-containing protein | |||||||||
Keywords | VIRUS / bacteriophage / virion / procapsid | |||||||||
Function / homology | Bacteriophage Mu, GpT / Mu-like prophage major head subunit gpT / Bacteriophage Mu GpT domain-containing protein Function and homology information | |||||||||
Biological species | Pseudomonas phage JBD30 (virus) | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.01 Å | |||||||||
Authors | Valentova, L. / Fuzik, T. / Plevka, P. | |||||||||
Funding support | European Union, Czech Republic, 2items
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Citation | Journal: Embo J. / Year: 2024 Title: Structure and replication of Pseudomonas aeruginosa phage JBD30 Authors: Valentova, L. / Plevka, P. / Fuzik, T. / Novacek, J. / Pospisil, J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8rkx.cif.gz | 356.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8rkx.ent.gz | 297.7 KB | Display | PDB format |
PDBx/mmJSON format | 8rkx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8rkx_validation.pdf.gz | 1.6 MB | Display | wwPDB validaton report |
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Full document | 8rkx_full_validation.pdf.gz | 1.6 MB | Display | |
Data in XML | 8rkx_validation.xml.gz | 82.8 KB | Display | |
Data in CIF | 8rkx_validation.cif.gz | 121.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rk/8rkx ftp://data.pdbj.org/pub/pdb/validation_reports/rk/8rkx | HTTPS FTP |
-Related structure data
Related structure data | 19285MC 8rk3C 8rk4C 8rk5C 8rk6C 8rk7C 8rk8C 8rk9C 8rkaC 8rkbC 8rkcC 8rknC 8rkoC 8rqeC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 33492.707 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Pseudomonas phage JBD30 (virus) / References: UniProt: L7P7W9 |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Pseudomonas phage JBD30 / Type: VIRUS Details: Phage JBD30 was propagated in P. aeruginosa strain BAA-28 and purified using CsCl gradient. Entity ID: all / Source: NATURAL | ||||||||||||||||||||
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Molecular weight | Value: 13.971 MDa / Experimental value: NO | ||||||||||||||||||||
Source (natural) | Organism: Pseudomonas phage JBD30 (virus) | ||||||||||||||||||||
Details of virus | Empty: NO / Enveloped: NO / Isolate: STRAIN / Type: VIRION | ||||||||||||||||||||
Natural host | Organism: Pseudomonas aeruginosa / Strain: BAA-28 | ||||||||||||||||||||
Virus shell | Name: JBD30 procapsid / Diameter: 570 nm / Triangulation number (T number): 7 | ||||||||||||||||||||
Buffer solution | pH: 8 / Details: 10 mM MgSO4, 10 mM NaCl, 50 mM Tris pH 8 | ||||||||||||||||||||
Buffer component |
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Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES / Details: phage titer 10^11 PFU | ||||||||||||||||||||
Specimen support | Details: Gatan Solarus II / Grid material: COPPER / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R2/1 | ||||||||||||||||||||
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277.15 K Details: blotting force 0, blotting time 2 s, waiting time 15 s |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal magnification: 130000 X / Nominal defocus max: 1600 nm / Nominal defocus min: 600 nm / Cs: 2.7 mm / C2 aperture diameter: 50 µm / Alignment procedure: COMA FREE |
Specimen holder | Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Image recording | Average exposure time: 7.5 sec. / Electron dose: 53 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Num. of grids imaged: 1 / Num. of real images: 13769 |
EM imaging optics | Energyfilter name: GIF Quantum LS / Energyfilter slit width: 20 eV |
Image scans | Width: 3838 / Height: 3710 / Movie frames/image: 40 / Used frames/image: 1-40 |
-Processing
EM software |
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CTF correction | Type: PHASE FLIPPING ONLY | ||||||||||||||||||||||||||||||||||||||||||||||||||
Particle selection | Num. of particles selected: 783 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Point symmetry: I (icosahedral) | ||||||||||||||||||||||||||||||||||||||||||||||||||
3D reconstruction | Resolution: 4.01 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 404 / Algorithm: FOURIER SPACE / Num. of class averages: 1 / Symmetry type: POINT | ||||||||||||||||||||||||||||||||||||||||||||||||||
Atomic model building | Protocol: OTHER / Space: REAL / Details: de novo model building | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
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