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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 8qk3 | ||||||
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タイトル | Human Adenovirus type 11 fiber knob in complex with its cell receptors, Desmoglein-2 and CD46 | ||||||
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![]() | VIRAL PROTEIN / adenovirus / cell entry / receptor binding | ||||||
機能・相同性 | ![]() sequestering of extracellular ligand from receptor / inner acrosomal membrane / Purkinje myocyte development / bundle of His cell-Purkinje myocyte adhesion involved in cell communication / cell adhesive protein binding involved in bundle of His cell-Purkinje myocyte communication / desmosome organization / Keratinization / regulation of Notch signaling pathway / desmosome / negative regulation of complement activation, classical pathway ...sequestering of extracellular ligand from receptor / inner acrosomal membrane / Purkinje myocyte development / bundle of His cell-Purkinje myocyte adhesion involved in cell communication / cell adhesive protein binding involved in bundle of His cell-Purkinje myocyte communication / desmosome organization / Keratinization / regulation of Notch signaling pathway / desmosome / negative regulation of complement activation, classical pathway / positive regulation of transforming growth factor beta production / T cell mediated immunity / Formation of the cornified envelope / cornified envelope / positive regulation of memory T cell differentiation / regulation of ventricular cardiac muscle cell action potential / positive regulation of regulatory T cell differentiation / Apoptotic cleavage of cell adhesion proteins / adhesion receptor-mediated virion attachment to host cell / regulation of heart rate by cardiac conduction / homophilic cell adhesion via plasma membrane adhesion molecules / positive regulation of interleukin-10 production / intercalated disc / RHOG GTPase cycle / lateral plasma membrane / single fertilization / RAC2 GTPase cycle / RAC3 GTPase cycle / maternal process involved in female pregnancy / positive regulation of T cell proliferation / cell adhesion molecule binding / complement activation, classical pathway / Regulation of Complement cascade / response to progesterone / cell-cell adhesion / viral capsid / cell-cell junction / cell junction / virus receptor activity / signaling receptor activity / adaptive immune response / cell adhesion / cadherin binding / symbiont entry into host cell / apical plasma membrane / negative regulation of gene expression / intracellular membrane-bounded organelle / innate immune response / focal adhesion / calcium ion binding / host cell nucleus / positive regulation of gene expression / cell surface / extracellular space / extracellular exosome / plasma membrane 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.2 Å | ||||||
![]() | Effantin, G. | ||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Toward the understanding of DSG2 and CD46 interaction with HAdV-11 fiber, a super-complex analysis. 著者: Gregory Effantin / Marc-André Hograindleur / Daphna Fenel / Pascal Fender / Emilie Vassal-Stermann / ![]() 要旨: The main limitation of oncolytic vectors is neutralization by blood components, which prevents intratumoral administration to patients. Enadenotucirev, a chimeric HAdV-11p/HAdV-3 adenovirus ...The main limitation of oncolytic vectors is neutralization by blood components, which prevents intratumoral administration to patients. Enadenotucirev, a chimeric HAdV-11p/HAdV-3 adenovirus identified by bio-selection, is a low seroprevalence vector active against a broad range of human carcinoma cell lines. At this stage, there's still some uncertainty about tropism and primary receptor utilization by HAdV-11. However, this information is very important, as it has a direct influence on the effectiveness of HAdV-11-based vectors. The aim of this work is to determine which of the two receptors, DSG2 and CD46, is involved in the attachment of the virus to the host, and what role they play in the early stages of infection. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 224.3 KB | 表示 | ![]() |
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PDB形式 | ![]() | 160.2 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 1.2 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 1.2 MB | 表示 | |
XML形式データ | ![]() | 38.2 KB | 表示 | |
CIF形式データ | ![]() | 57.9 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 18455MC ![]() 8qjxC ![]() 8qjyC C: 同じ文献を引用 ( M: このデータのモデリングに利用したマップデータ |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
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集合体
登録構造単位 | ![]()
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要素
#1: タンパク質 | 分子量: 35564.773 Da / 分子数: 3 / 由来タイプ: 組換発現 由来: (組換発現) ![]() 遺伝子: L5 / 発現宿主: ![]() ![]() #2: タンパク質 | | 分子量: 43795.164 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() #3: タンパク質 | | 分子量: 122421.633 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 | 名称: Complex between the human adenovirus 11 fiber knob, the human desmoglein 2 (domains ec2/ec3) and the human CD46 (domains SCR1 and SCR2) タイプ: COMPLEX / Entity ID: all / 由来: RECOMBINANT |
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分子量 | 実験値: NO |
由来(天然) | 生物種: ![]() ![]() |
由来(組換発現) | 生物種: ![]() ![]() |
緩衝液 | pH: 8 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE |
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電子顕微鏡撮影
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2500 nm / 最小 デフォーカス(公称値): 1000 nm |
撮影 | 電子線照射量: 58 e/Å2 フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) |
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解析
CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3次元再構成 | 解像度: 3.2 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 180510 / 対称性のタイプ: POINT | ||||||||||||||||||||||||
拘束条件 |
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