- PDB-8otz: 48-nm repeat of the native axonemal doublet microtubule from bovi... -
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基本情報
登録情報
データベース: PDB / ID: 8otz
タイトル
48-nm repeat of the native axonemal doublet microtubule from bovine sperm
要素
(Cilia- and flagella-associated protein ...) x 11
(EF-hand domain-containing ...) x 4
(Outer dense fiber of sperm tails ...) x 3
(Piercer of microtubule wall ...) x 2
(Stabilizer of axonemal microtubules ...) x 2
(Testis expressed ...) x 2
(Testis-expressed ...) x 2
(Uncharacterized protein ...) x 4
ATP6V1F neighbor
CFAP97 domain containing 1
Chromosome 13 C20orf85 homolog
Chromosome 19 C17orf98 homolog
Chromosome 20 C5orf49 homolog
Chromosome 7 open reading frame 31
Cilia and flagella associated protein 77
Cilia- and flagella- associated protein 210
Coiled-coil domain-containing protein 105
Dual specificity phosphatase 21
EF-hand calcium-binding domain-containing protein 3
Enkurin
Family with sequence similarity 166 member A
Meiosis-specific nuclear structural protein 1
Nucleoside diphosphate kinase 7
Parkin coregulated gene protein
Protein FAM166C
Protein Flattop
Protein phosphatase 1 regulatory subunit 32
RIB43A-like with coiled-coils protein 2
Sperm acrosome associated 9
Sperm-associated antigen 8
Spermatid-specific manchette-related protein 1
Spermatogenesis-associated protein 48
TEPP protein
Tektin bundle interacting protein 1
Tektin-1
Tektin-2
Tektin-3
Tektin-4
Tektin-5
Testis specific serine kinase 6
Theg spermatid protein like
Tubulin alpha-3 chain
Tubulin beta-4B chain
UPF0602 protein C4orf47 homolog
キーワード
STRUCTURAL PROTEIN / microtubule / cilia / sperm / axoneme
機能・相同性
機能・相同性情報
sperm flagellum assembly / outer acrosomal membrane / regulation of brood size / establishment of left/right asymmetry / manchette assembly / axonemal B tubule inner sheath / axonemal A tubule inner sheath / protein polyglutamylation / sperm axoneme assembly / positive regulation of feeding behavior ...sperm flagellum assembly / outer acrosomal membrane / regulation of brood size / establishment of left/right asymmetry / manchette assembly / axonemal B tubule inner sheath / axonemal A tubule inner sheath / protein polyglutamylation / sperm axoneme assembly / positive regulation of feeding behavior / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Intraflagellar transport / Carboxyterminal post-translational modifications of tubulin / Sealing of the nuclear envelope (NE) by ESCRT-III / Kinesins / Resolution of Sister Chromatid Cohesion / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / cilium-dependent cell motility / COPI-dependent Golgi-to-ER retrograde traffic / COPI-independent Golgi-to-ER retrograde traffic / MAP kinase tyrosine/serine/threonine phosphatase activity / COPI-mediated anterograde transport / RHO GTPases activate IQGAPs / regulation of cilium beat frequency involved in ciliary motility / RHO GTPases Activate Formins / cilium movement involved in cell motility / 転移酵素; リンを含む基を移すもの / acrosomal membrane / ciliary transition zone / cilium movement / MHC class II antigen presentation / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / axoneme assembly / axonemal microtubule / Aggrephagy / sperm DNA condensation / flagellated sperm motility / cilium organization / The role of GTSE1 in G2/M progression after G2 checkpoint / Separation of Sister Chromatids / gamma-tubulin ring complex / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / Recruitment of NuMA to mitotic centrosomes / manchette / nucleotide-activated protein kinase complex / Regulation of PLK1 Activity at G2/M Transition / Hedgehog 'off' state / UTP biosynthetic process / CTP biosynthetic process / motile cilium / negative regulation of TOR signaling / positive regulation of cell motility / determination of left/right symmetry / GTP biosynthetic process / microtubule organizing center / Neutrophil degranulation / nucleoside diphosphate kinase activity / ciliary base / myosin phosphatase activity / dephosphorylation / regulation of neuron projection development / beta-tubulin binding / axoneme / mitotic cytokinesis / spermatid development / centriolar satellite / cilium assembly / cellular response to UV-C / single fertilization / sperm flagellum / alpha-tubulin binding / phosphatase binding / cellular response to glucose starvation / sperm midpiece / 3'-5' exonuclease activity / centriole / Hsp70 protein binding / acrosomal vesicle / mitotic spindle organization / ciliary basal body / protein tyrosine phosphatase activity / meiotic cell cycle / cell projection / G protein-coupled receptor binding / Hsp90 protein binding / mitotic spindle / cilium / structural constituent of cytoskeleton / spindle / spindle pole / microtubule cytoskeleton organization / SH3 domain binding / calcium-dependent protein binding / calcium ion transport 類似検索 - 分子機能
Protein of unknown function DUF4542 / Uncharacterized protein C1orf100 / Sperm microtubule associated protein 1 / Family of unknown function (DUF5530) / Testicular haploid expressed repeat / Uncharacterised protein family C20orf85 / Protein of unknown function DUF4562 / Spermatid-specific manchette-related protein 1 / Testis-expressed protein 33 / Protein of unknown function DUF4586 ...Protein of unknown function DUF4542 / Uncharacterized protein C1orf100 / Sperm microtubule associated protein 1 / Family of unknown function (DUF5530) / Testicular haploid expressed repeat / Uncharacterised protein family C20orf85 / Protein of unknown function DUF4562 / Spermatid-specific manchette-related protein 1 / Testis-expressed protein 33 / Protein of unknown function DUF4586 / Testis-expressed sequence 37 protein / Hemingway/CFA97 / CFAP97 domain-containing protein 1/2 / Testicular haploid expressed gene protein-like / Testis-specific serine/threonine-protein kinase 6, catalytic domain / C10orf82-like / Hemingway/CFA97 / Testicular haploid expressed repeat / Normal lung function maintenance, Low in Lung Cancer 1 protein / Domain of unknown function (DUF4562) / Spermatid-specific manchette-related protein 1 / TSC21 family / Cilia-and flagella-associated protein 96 / Testis-expressed sequence 33 protein family / Repeats in THEG (testicular haploid expressed gene) and several fly proteins. / Protein phosphatase 1 regulatory subunit 32 / Testis, prostate and placenta-expressed protein / Testis-expressed protein 49 / Protein phosphatase 1 regulatory subunit 32 / Cilia- and flagella-associated protein 143 / Cilia and flagella associated protein 107 / Protein of unknown function DUF1143 / Atypical dual specificity phosphatase, subfamily B / Protein of unknown function DUF4541 / Protein of unknown function DUF4513 / Cilia- and flagella-associated protein 68 / Sperm-associated microtubule inner protein 10 / Cilia and flagella associated protein 90 / Sperm-tail PG-rich repeat / DJBP, EF-hand domain / Tektin bundle interacting protein 1 / Coiled-coil domain-containing protein 105 / Sperm-tail PG-rich repeat / EF-hand domain / Tektin bundle interacting protein 1 / FAM183 family / FAM183A and FAM183B related / Sperm-associated antigen 8 / Sperm acrosome-associated protein 9 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain / Cilia- and flagella-associated protein 107 / : / Sperm acrosome-associated protein 9 / Protein CFAP95 / Tektin / Cilia- and flagella-associated protein 276 / Tektin family / Protein of unknown function (DUF3695) / Cilia- and flagella-associated protein 77 / Cilia- and flagella-associated protein 77 / Uncharacterised protein FAM166/UPF0605 / Ciliary microtubule inner protein 2B-like / Nucleoside diphosphate kinase 7 / Meiosis-specific nuclear structural protein 1 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / : / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / Calmodulin-binding / Enkurin domain profile. / : / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / Parkin co-regulated protein / Parkin co-regulated protein / Dual specificity phosphatase, catalytic domain / Nucleoside diphosphate kinase (NDPK)-like domain profile. / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / Dual specificity protein phosphatase domain / Dual specificity protein phosphatase domain profile. / Nucleoside diphosphate kinase / Nucleoside diphosphate kinase-like domain 類似検索 - ドメイン・相同性
GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Uncharacterized protein / Piercer of microtubule wall 2 protein / Cilia and flagella associated protein 77 / Chromosome 20 C5orf49 homolog / Theg spermatid protein like / Sperm microtubule inner protein 11 / Sperm acrosome associated 9 / EF-hand domain-containing family member C2 ...GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Uncharacterized protein / Piercer of microtubule wall 2 protein / Cilia and flagella associated protein 77 / Chromosome 20 C5orf49 homolog / Theg spermatid protein like / Sperm microtubule inner protein 11 / Sperm acrosome associated 9 / EF-hand domain-containing family member C2 / Chromosome 13 C20orf85 homolog / Protein SPMIP9 / Parkin coregulated gene protein / Tektin-3 / Sperm-associated microtubule inner protein 5 / Cilia- and flagella-associated protein 144 / Sperm-associated microtubule inner protein 10 / TEPP protein / Enkurin / Cilia- and flagella-associated protein 276 / Chromosome 19 C17orf98 homolog / Cilia- and flagella- associated protein 210 / Cilia- and flagella-associated protein 52 / EF-hand domain-containing protein 1 / Sperm-associated antigen 8 / EF-hand domain-containing family member B / Tektin bundle interacting protein 1 / Testis specific serine kinase 6 / Cilia- and flagella-associated protein 53 / Cilia- and flagella-associated protein 161 / CFAP97 domain containing 1 / Outer dense fiber of sperm tails 3 / Ciliary microtubule inner protein 2A / ATP6V1F neighbor / EF-hand domain-containing protein / Dual specificity phosphatase 21 / Meiosis-specific nuclear structural protein 1 / Protein SPMIP2 / Cilia-and flagella-associated protein 96 / EF-hand calcium-binding domain-containing protein 3 / Cilia- and flagella-associated protein 68 / Tektin-2 / Stabilizer of axonemal microtubules 4 / Cilia- and flagella-associated protein 107 / Tektin-4 / Tektin-5 / Tektin-like protein 1 / Tubulin alpha-3 chain / Sperm microtubule inner protein 6 / Tektin-1 / Protein SPMIP3 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 95 / Testis expressed 33 / RIB43A-like with coiled-coils protein 2 / Cilia- and flagella-associated protein 45 / Piercer of microtubule wall 1 protein / Tubulin beta-4B chain / Ciliary microtubule inner protein 2C / Nucleoside diphosphate kinase homolog 7 / Cilia- and flagella-associated protein 20 類似検索 - 構成要素
Netherlands Organisation for Scientific Research (NWO)
740.018.007
オランダ
引用
ジャーナル: Cell / 年: 2023 タイトル: Structural specializations of the sperm tail. 著者: Miguel Ricardo Leung / Jianwei Zeng / Xiangli Wang / Marc C Roelofs / Wei Huang / Riccardo Zenezini Chiozzi / Johannes F Hevler / Albert J R Heck / Susan K Dutcher / Alan Brown / Rui Zhang / ...著者: Miguel Ricardo Leung / Jianwei Zeng / Xiangli Wang / Marc C Roelofs / Wei Huang / Riccardo Zenezini Chiozzi / Johannes F Hevler / Albert J R Heck / Susan K Dutcher / Alan Brown / Rui Zhang / Tzviya Zeev-Ben-Mordehai / 要旨: Sperm motility is crucial to reproductive success in sexually reproducing organisms. Impaired sperm movement causes male infertility, which is increasing globally. Sperm are powered by a microtubule- ...Sperm motility is crucial to reproductive success in sexually reproducing organisms. Impaired sperm movement causes male infertility, which is increasing globally. Sperm are powered by a microtubule-based molecular machine-the axoneme-but it is unclear how axonemal microtubules are ornamented to support motility in diverse fertilization environments. Here, we present high-resolution structures of native axonemal doublet microtubules (DMTs) from sea urchin and bovine sperm, representing external and internal fertilizers. We identify >60 proteins decorating sperm DMTs; at least 15 are sperm associated and 16 are linked to infertility. By comparing DMTs across species and cell types, we define core microtubule inner proteins (MIPs) and analyze evolution of the tektin bundle. We identify conserved axonemal microtubule-associated proteins (MAPs) with unique tubulin-binding modes. Additionally, we identify a testis-specific serine/threonine kinase that links DMTs to outer dense fibers in mammalian sperm. Our study provides structural foundations for understanding sperm evolution, motility, and dysfunction at a molecular level.