+
データを開く
-
基本情報
登録情報 | データベース: PDB / ID: 8jqi | ||||||
---|---|---|---|---|---|---|---|
タイトル | Cryo EM map of full length PLC gamma 2 and FGFR1 Kinase Domain | ||||||
![]() |
| ||||||
![]() | HYDROLASE / PLCg2 / PLC gamma 2 | ||||||
機能・相同性 | ![]() inositol trisphosphate biosynthetic process / regulation of calcineurin-NFAT signaling cascade / positive regulation of dendritic cell cytokine production / follicular B cell differentiation / Signaling by FGFR1 amplification mutants / negative regulation of fibroblast growth factor production / positive regulation of mitotic cell cycle DNA replication / regulation of extrinsic apoptotic signaling pathway in absence of ligand / Signaling by plasma membrane FGFR1 fusions / diphosphate metabolic process ...inositol trisphosphate biosynthetic process / regulation of calcineurin-NFAT signaling cascade / positive regulation of dendritic cell cytokine production / follicular B cell differentiation / Signaling by FGFR1 amplification mutants / negative regulation of fibroblast growth factor production / positive regulation of mitotic cell cycle DNA replication / regulation of extrinsic apoptotic signaling pathway in absence of ligand / Signaling by plasma membrane FGFR1 fusions / diphosphate metabolic process / phosphoinositide phospholipase C / regulation of phosphate transport / regulation of lateral mesodermal cell fate specification / FGFR1c and Klotho ligand binding and activation / antifungal innate immune response / vitamin D3 metabolic process / cementum mineralization / positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway / positive regulation of interleukin-23 production / cellular response to lectin / regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling / receptor-receptor interaction / response to sodium phosphate / fibroblast growth factor receptor signaling pathway involved in orbitofrontal cortex development / response to yeast / ventricular zone neuroblast division / phosphorylation-dependent protein binding / Epithelial-Mesenchymal Transition (EMT) during gastrulation / auditory receptor cell development / chordate embryonic development / positive regulation of phospholipase activity / phosphatidylinositol metabolic process / Toll Like Receptor 4 (TLR4) Cascade / positive regulation of cell cycle G1/S phase transition / positive regulation of parathyroid hormone secretion / mesenchymal cell proliferation / paraxial mesoderm development / positive regulation of phagocytosis, engulfment / regulation of postsynaptic density assembly / positive regulation of neuroinflammatory response / cell activation / FGFR1b ligand binding and activation / Signaling by activated point mutants of FGFR1 / Erythropoietin activates Phospholipase C gamma (PLCG) / FGFR1c ligand binding and activation / phospholipase C activity / organ induction / Downstream signaling of activated FGFR1 / branching involved in salivary gland morphogenesis / Phospholipase C-mediated cascade: FGFR1 / phosphatidylinositol-4,5-bisphosphate phospholipase C activity / lung-associated mesenchyme development / phosphatidylinositol biosynthetic process / programmed cell death / cell projection assembly / macrophage activation involved in immune response / phospholipid catabolic process / cellular response to lipid / outer ear morphogenesis / embryonic limb morphogenesis / regulation of canonical NF-kappaB signal transduction / negative regulation of programmed cell death / positive regulation of endothelial cell chemotaxis / positive regulation of vascular endothelial cell proliferation / positive regulation of mesenchymal cell proliferation / positive regulation of macrophage cytokine production / ureteric bud development / skeletal system morphogenesis / cardiac muscle cell proliferation / middle ear morphogenesis / inner ear morphogenesis / toll-like receptor signaling pathway / phosphatidylinositol-mediated signaling / PI-3K cascade:FGFR1 / Formation of paraxial mesoderm / Dectin-2 family / positive regulation of stem cell proliferation / intracellular vesicle / positive regulation of NLRP3 inflammasome complex assembly / Synthesis of IP3 and IP4 in the cytosol / midbrain development / positive regulation of reactive oxygen species biosynthetic process / stimulatory C-type lectin receptor signaling pathway / Fc-epsilon receptor signaling pathway / fibroblast growth factor binding / B cell activation / regulation of cell differentiation / positive regulation of interleukin-10 production / regulation of lipid metabolic process / positive regulation of receptor internalization / Generation of second messenger molecules / PI3K Cascade / positive regulation of epithelial cell migration / epithelial to mesenchymal transition / positive regulation of blood vessel endothelial cell migration / fibroblast growth factor receptor signaling pathway / positive regulation of type I interferon production / Role of phospholipids in phagocytosis / chondrocyte differentiation / positive regulation of MAP kinase activity 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.1 Å | ||||||
![]() | Shin, Y.-C. / Liao, M. | ||||||
資金援助 | 1件
| ||||||
![]() | ![]() タイトル: The crystal and cryo-EM structures of PLCγ2 reveal dynamic interdomain recognitions in autoinhibition. 著者: Young-Cheul Shin / Ashlee Marie Plummer-Medeiros / Alison Mungenast / Hyeong-Wook Choi / Karen TenDyke / Xiaojie Zhu / Jennifer Shepard / Kristen Sanders / Ningning Zhuang / Liang Hu / ...著者: Young-Cheul Shin / Ashlee Marie Plummer-Medeiros / Alison Mungenast / Hyeong-Wook Choi / Karen TenDyke / Xiaojie Zhu / Jennifer Shepard / Kristen Sanders / Ningning Zhuang / Liang Hu / Dongming Qian / Kangkang Song / Chen Xu / John Wang / Suresh B Poda / Maofu Liao / Yu Chen / ![]() ![]() 要旨: Phospholipase C gamma 2 (PLCγ2) plays important roles in cell signaling downstream of various membrane receptors. PLCγ2 contains a multidomain inhibitory region critical for its regulation, while ...Phospholipase C gamma 2 (PLCγ2) plays important roles in cell signaling downstream of various membrane receptors. PLCγ2 contains a multidomain inhibitory region critical for its regulation, while it has remained unclear how these domains contribute to PLCγ2 activity modulation. Here we determined three structures of human PLCγ2 in autoinhibited states, which reveal dynamic interactions at the autoinhibition interface, involving the conformational flexibility of the Src homology 3 (SH3) domain in the inhibitory region, and its previously unknown interaction with a carboxyl-terminal helical domain in the core region. We also determined a structure of PLCγ2 bound to the kinase domain of fibroblast growth factor receptor 1 (FGFR1), which demonstrates the recognition of FGFR1 by the nSH2 domain in the inhibitory region of PLCγ2. Our results provide structural insights into PLCγ2 regulation that will facilitate future mechanistic studies to understand the entire activation process. #1: ![]() タイトル: The crystal and cryo-EM structures of PLC gamma 2 reveal dynamic inter-domain recognitions in autoinhibition 著者: Shin, Y.C. / Plummer-Medeiros, A.M. / Mungenast, A. / Choi, H.W. / TenDyke, K. / Zhu, X. / Shepard, J. / Zhuang, N. / Hu, L. / Qian, D. / Song, K. / Xu, C. / Wang, J. / Poda, S.B. / Liao, M. / Chen, Y. | ||||||
履歴 |
|
-
構造の表示
構造ビューア | 分子: ![]() ![]() |
---|
-
ダウンロードとリンク
-
ダウンロード
PDBx/mmCIF形式 | ![]() | 272.1 KB | 表示 | ![]() |
---|---|---|---|---|
PDB形式 | ![]() | 208.8 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 1.2 MB | 表示 | ![]() |
---|---|---|---|---|
文書・詳細版 | ![]() | 1.2 MB | 表示 | |
XML形式データ | ![]() | 55.1 KB | 表示 | |
CIF形式データ | ![]() | 80.7 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
-
リンク
-
集合体
登録構造単位 | ![]()
|
---|---|
1 |
|
-
要素
#1: タンパク質 | 分子量: 148074.578 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() |
---|---|
#2: タンパク質 | 分子量: 91978.680 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() |
Has protein modification | N |
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
---|---|
EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-
試料調製
構成要素 | 名称: PLCg2 and FGFR1 / タイプ: COMPLEX / Entity ID: all / 由来: MULTIPLE SOURCES |
---|---|
分子量 | 値: 0.1478 MDa / 実験値: NO |
由来(天然) | 生物種: ![]() |
由来(組換発現) | 生物種: ![]() |
緩衝液 | pH: 7.9 / 詳細: 25 mM Tris pH 7.9, 150 mM NaCl, 1mM TCEP |
試料 | 濃度: 2.7 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE |
-
電子顕微鏡撮影
実験機器 | ![]() モデル: Talos Arctica / 画像提供: FEI Company |
---|---|
顕微鏡 | モデル: FEI TALOS ARCTICA |
電子銃 | 電子線源: ![]() |
電子レンズ | モード: OTHER / 最大 デフォーカス(公称値): 2200 nm / 最小 デフォーカス(公称値): 1000 nm |
撮影 | 電子線照射量: 53.0932 e/Å2 / フィルム・検出器のモデル: GATAN K3 (6k x 4k) |
-
解析
CTF補正 | タイプ: NONE |
---|---|
3次元再構成 | 解像度: 4.1 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 45439 / 対称性のタイプ: POINT |