+Open data
-Basic information
Entry | Database: PDB / ID: 8jnl | ||||||
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Title | Psb34 from red algal Porphyridium purpureum. | ||||||
Components | Psb34 | ||||||
Keywords | PHOTOSYNTHESIS / Psb34 | ||||||
Function / homology | membrane / Uncharacterized protein Function and homology information | ||||||
Biological species | Porphyridium purpureum (eukaryote) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.2 Å | ||||||
Authors | You, X. / Zhang, X. / Cheng, J. / Xiao, Y.N. / Sun, S. / Sui, S.F. | ||||||
Funding support | 1items
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Citation | Journal: To Be Published Title: Structure of lateral hexamer of PBS-PSII-PSI-LHCs megacomplex at 6.3 Angstroms resolution. Authors: You, X. / Zhang, X. / Cheng, J. / Xiao, Y.N. / Sun, S. / Sui, S.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8jnl.cif.gz | 15.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8jnl.ent.gz | 7.3 KB | Display | PDB format |
PDBx/mmJSON format | 8jnl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8jnl_validation.pdf.gz | 1.5 MB | Display | wwPDB validaton report |
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Full document | 8jnl_full_validation.pdf.gz | 1.5 MB | Display | |
Data in XML | 8jnl_validation.xml.gz | 18.6 KB | Display | |
Data in CIF | 8jnl_validation.cif.gz | 23.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jn/8jnl ftp://data.pdbj.org/pub/pdb/validation_reports/jn/8jnl | HTTPS FTP |
-Related structure data
Related structure data | 33597MC 8jngC 8jnmC 8jnnC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 9873.483 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Porphyridium purpureum (eukaryote) / Gene: FVE85_2199 / Production host: Porphyridium purpureum (eukaryote) / References: UniProt: A0A5J4YZ83 |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: CELL / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: PSII subunit Psb34 from Porphyridium purpureum. / Type: CELL / Entity ID: all / Source: NATURAL |
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Source (natural) | Organism: Porphyridium purpureum (eukaryote) |
Buffer solution | pH: 7.6 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: SPOT SCAN |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 6000 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 35 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING ONLY |
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3D reconstruction | Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 762000 / Symmetry type: POINT |