National Natural Science Foundation of China (NSFC)
32171187
中国
引用
ジャーナル: Cell Res / 年: 2023 タイトル: Structural basis of signaling regulation of the human melanocortin-2 receptor by MRAP1. 著者: Ping Luo / Wenbo Feng / Shanshan Ma / Antao Dai / Kai Wu / Xianyue Chen / Qingning Yuan / Xiaoqing Cai / Dehua Yang / Ming-Wei Wang / H Eric Xu / Yi Jiang / 要旨: G protein-coupled receptors (GPCRs) are regulated by various downstream proteins, of which the melanocortin receptor accessory protein 1 (MRAP1) is closely involved in the regulation of melanocortin ...G protein-coupled receptors (GPCRs) are regulated by various downstream proteins, of which the melanocortin receptor accessory protein 1 (MRAP1) is closely involved in the regulation of melanocortin receptor 2 (MC2R). Assisted by MRAP1, MC2R responds to adrenocorticotropic hormone (ACTH) and stimulates glucocorticoid biogenesis and cortisol secretion. MC2R activation plays an essential role in the hypothalamic-pituitary-adrenal (HPA) axis that regulates stress response, while its dysfunction causes glucocorticoid insufficiency- or cortisol excess-associated disorders. Here, we present a cryo-electron microscopy (cryo-EM) structure of the ACTH-bound MC2R-G-MRAP1 complex. Our structure, together with mutagenesis analysis, reveals a unique sharp kink at the extracellular region of MRAP1 and the 'seat-belt' effect of MRAP1 on stabilizing ACTH binding and MC2R activation. Mechanisms of ACTH recognition by MC2R and receptor activation are also demonstrated. These findings deepen our understanding of GPCR regulation by accessory proteins and provide valuable insights into the ab initio design of therapeutic agents targeting MC2R.
履歴
登録
2022年9月21日
登録サイト: PDBJ / 処理サイト: PDBE
改定 1.0
2023年1月11日
Provider: repository / タイプ: Initial release
改定 1.0
2023年1月11日
Data content type: EM metadata / Data content type: EM metadata / Provider: repository / タイプ: Initial release
改定 1.0
2023年1月11日
Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / タイプ: Initial release
改定 1.0
2023年1月11日
Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / タイプ: Initial release
改定 1.0
2023年1月11日
Data content type: Image / Data content type: Image / Provider: repository / タイプ: Initial release
改定 1.0
2023年1月11日
Data content type: Primary map / Data content type: Primary map / Provider: repository / タイプ: Initial release
改定 1.0
2023年1月11日
Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / タイプ: Initial release
改定 1.0
2023年1月11日
Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / タイプ: Initial release
改定 1.0
2023年1月11日
Data content type: Image / Data content type: Image / Provider: repository / タイプ: Initial release
改定 1.0
2023年1月11日
Data content type: Primary map / Data content type: Primary map / Provider: repository / タイプ: Initial release
改定 1.0
2023年1月11日
Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / タイプ: Initial release
改定 1.0
2023年1月11日
Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / タイプ: Initial release
改定 1.0
2023年1月11日
Data content type: Image / Data content type: Image / Provider: repository / タイプ: Initial release
改定 1.0
2023年1月11日
Data content type: Primary map / Data content type: Primary map / Provider: repository / タイプ: Initial release
Data content type: EM metadata / Data content type: EM metadata / EM metadata / Group: Data processing / Experimental summary / Data content type: EM metadata / EM metadata / カテゴリ: em_admin / em_software / Data content type: EM metadata / EM metadata / Item: _em_admin.last_update / _em_software.name
Guanine nucleotide-binding protein ... , 3種, 3分子 ABG
#1: タンパク質
Guaninenucleotide-bindingproteinG(i) subunitalpha-3,Guaninenucleotide-bindingproteinG(s) subunitalphaisoformsXLas / G(i) alpha-3 / Adenylate cyclase-stimulating G alpha protein / Extra large alphas protein / XLalphas