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-基本情報
登録情報 | データベース: PDB / ID: 8fte | ||||||
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タイトル | CryoEM strucutre of 22-mer RBM2 of the Salmonella MS-ring | ||||||
要素 | Flagellar M-ring protein | ||||||
キーワード | MOTOR PROTEIN / flagellar / 22-fold / ring-building motif / MS-ring / FliF | ||||||
機能・相同性 | 機能・相同性情報 bacterial-type flagellum basal body, MS ring / cytoskeletal motor activity / bacterial-type flagellum-dependent cell motility / plasma membrane 類似検索 - 分子機能 | ||||||
生物種 | Salmonella enterica subsp. enterica serovar Typhimurium (サルモネラ菌) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.8 Å | ||||||
データ登録者 | Singh, P.S. / Nakagawa, T. / Cecchini, G. / Iverson, T.M. | ||||||
資金援助 | 米国, 1件
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引用 | ジャーナル: PLoS One / 年: 2023 タイトル: CryoEM structure of a post-assembly MS-ring reveals plasticity in stoichiometry and conformation. 著者: Prashant K Singh / Gary Cecchini / Terunaga Nakagawa / T M Iverson / 要旨: The flagellar motor supports bacterial chemotaxis, a process that allows bacteria to move in response to their environment. A central feature of this motor is the MS-ring, which is composed entirely ...The flagellar motor supports bacterial chemotaxis, a process that allows bacteria to move in response to their environment. A central feature of this motor is the MS-ring, which is composed entirely of repeats of the FliF subunit. This MS-ring is critical for the assembly and stability of the flagellar switch and the entire flagellum. Despite multiple independent cryoEM structures of the MS-ring, there remains a debate about the stoichiometry and organization of the ring-building motifs (RBMs). Here, we report the cryoEM structure of a Salmonella MS-ring that was purified from the assembled flagellar switch complex (MSC-ring). We term this the 'post-assembly' state. Using 2D class averages, we show that under these conditions, the post-assembly MS-ring can contain 32, 33, or 34 FliF subunits, with 33 being the most common. RBM3 has a single location with C32, C33, or C34 symmetry. RBM2 is found in two locations with RBM2inner having C21 or C22 symmetry and an RBM2outer-RBM1 having C11 symmetry. Comparison to previously reported structures identifies several differences. Most strikingly, we find that the membrane domain forms 11 regions of discrete density at the base of the structure rather than a contiguous ring, although density could not be unambiguously interpreted. We further find density in some previously unresolved areas, and we assigned amino acids to those regions. Finally, we find differences in interdomain angles in RBM3 that affect the diameter of the ring. Together, these investigations support a model of the flagellum with structural plasticity, which may be important for flagellar assembly and function. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 8fte.cif.gz | 441.7 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb8fte.ent.gz | 301.9 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 8fte.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 8fte_validation.pdf.gz | 1.4 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 8fte_full_validation.pdf.gz | 1.5 MB | 表示 | |
XML形式データ | 8fte_validation.xml.gz | 59.6 KB | 表示 | |
CIF形式データ | 8fte_validation.cif.gz | 94.8 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/ft/8fte ftp://data.pdbj.org/pub/pdb/validation_reports/ft/8fte | HTTPS FTP |
-関連構造データ
関連構造データ | 29424MC 8ftfC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
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非結晶学的対称性 (NCS) | NCSドメイン:
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