National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI156636
米国
引用
ジャーナル: Sci Adv / 年: 2022 タイトル: Structure and host specificity of bacteriophage Andhra. 著者: N'Toia C Hawkins / James L Kizziah / Asma Hatoum-Aslan / Terje Dokland / 要旨: is an opportunistic pathogen of the human skin, often associated with infections of implanted medical devices. Staphylococcal picoviruses are a group of strictly lytic, short-tailed bacteriophages ... is an opportunistic pathogen of the human skin, often associated with infections of implanted medical devices. Staphylococcal picoviruses are a group of strictly lytic, short-tailed bacteriophages with compact genomes that are attractive candidates for therapeutic use. Here, we report the structure of the complete virion of -infecting phage Andhra, determined using high-resolution cryo-electron microscopy, allowing atomic modeling of 11 capsid and tail proteins. The capsid is a = 4 icosahedron containing a unique stabilizing capsid lining protein. The tail includes 12 trimers of a unique receptor binding protein (RBP), a lytic protein that also serves to anchor the RBPs to the tail stem, and a hexameric tail knob that acts as a gatekeeper for DNA ejection. Using structure prediction with AlphaFold, we identified the two proteins that comprise the tail tip heterooctamer. Our findings elucidate critical features for virion assembly, host recognition, and penetration.
履歴
登録
2022年9月13日
登録サイト: RCSB / 処理サイト: RCSB
改定 1.0
2022年12月14日
Provider: repository / タイプ: Initial release
改定 1.1
2024年6月19日
Group: Data collection / カテゴリ: chem_comp_atom / chem_comp_bond
H: gp19, capsid lining protein G: gp19, capsid lining protein A: Major capsid protein B: Major capsid protein C: Major capsid protein D: Major capsid protein F: gp19, capsid lining protein E: gp19, capsid lining protein
H: gp19, capsid lining protein G: gp19, capsid lining protein A: Major capsid protein B: Major capsid protein C: Major capsid protein D: Major capsid protein F: gp19, capsid lining protein E: gp19, capsid lining protein
H: gp19, capsid lining protein G: gp19, capsid lining protein A: Major capsid protein B: Major capsid protein C: Major capsid protein D: Major capsid protein F: gp19, capsid lining protein E: gp19, capsid lining protein
x 5
icosahedral pentamer
1.07 MDa, 40 ポリマー
分子量 (理論値)
分子数
合計 (水以外)
1,069,895
40
ポリマ-
1,069,895
40
非ポリマー
0
0
水
0
タイプ
名称
対称操作
数
identity operation
1_555
x,y,z
1
point symmetry operation
4
4
H: gp19, capsid lining protein G: gp19, capsid lining protein A: Major capsid protein B: Major capsid protein C: Major capsid protein D: Major capsid protein F: gp19, capsid lining protein E: gp19, capsid lining protein