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Open data
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Basic information
Entry | Database: PDB / ID: 8akx | ||||||
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Title | 305 A SynPspA rod after incubation with ATP | ||||||
![]() | Chloroplast membrane-associated 30 kD protein | ||||||
![]() | LIPID BINDING PROTEIN / ![]() | ||||||
Function / homology | phage shock / PspA/IM30 / PspA/IM30 family / ![]() ![]() | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Junglas, B. / Hudina, E. / Schoennenbeck, P. / Ritter, I. / Santiago-Schuebel, B. / Huesgen, P. / Sachse, C. | ||||||
Funding support | European Union, 1items
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![]() | ![]() Title: Non-canonical ATPase activity drives PspA membrane constriction Authors: Junglas, B. / Hudina, E. / Schoennenbeck, P. / Ritter, I. / Santiago-Schuebel, B. / Huesgen, P. / Sachse, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 3.7 MB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 15496MC ![]() 8akqC ![]() 8akrC ![]() 8aksC ![]() 8aktC ![]() 8akuC ![]() 8akvC ![]() 8akwC ![]() 8akyC ![]() 8akzC ![]() 8al0C M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein | Mass: 28097.758 Da / Num. of mol.: 60 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Details (production host): Based on pET50b(+) with deletion of NusA tag Production host: ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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EM experiment | Aggregation state: HELICAL ARRAY / 3D reconstruction method: helical reconstruction |
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Sample preparation
Component | Name: Phage shock protein A (PspA) / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Molecular weight | Value: 162.2 kDa/nm / Experimental value: NO |
Source (natural) | Organism: ![]() ![]() ![]() |
Source (recombinant) | Organism: ![]() ![]() ![]() ![]() |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied![]() ![]() |
Vitrification![]() | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
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Microscopy | Model: FEI TALOS ARCTICA |
Electron gun | Electron source![]() ![]() |
Electron lens | Mode: BRIGHT FIELD![]() |
Image recording | Electron dose: 58 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
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Processing
Software | Name: UCSF ChimeraX / Version: 1.3/v9 / Classification: model building / URL: https://www.rbvi.ucsf.edu/chimerax/ / Os: macOS / Type: package |
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CTF correction![]() | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
Helical symmerty | Angular rotation/subunit: -85 ° / Axial rise/subunit: 1.64 Å / Axial symmetry: C1 |
3D reconstruction![]() | Resolution: 6.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 25901 / Symmetry type: HELICAL |