+Open data
-Basic information
Entry | Database: PDB / ID: 8aky | ||||||
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Title | 290 A SynPspA rod after incubation with ATP | ||||||
Components | Chloroplast membrane-associated 30 kD protein | ||||||
Keywords | LIPID BINDING PROTEIN / Nucleotide binding / Helical assembly / ESCRT-III fold / Membrane remodeling | ||||||
Function / homology | phage shock / PspA/IM30 / PspA/IM30 family / cytosol / Chloroplast membrane-associated 30 kD protein Function and homology information | ||||||
Biological species | Synechocystis sp. PCC 6803 (bacteria) | ||||||
Method | ELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 4.7 Å | ||||||
Authors | Junglas, B. / Hudina, E. / Schoennenbeck, P. / Ritter, I. / Santiago-Schuebel, B. / Huesgen, P. / Sachse, C. | ||||||
Funding support | European Union, 1items
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Citation | Journal: To Be Published Title: Non-canonical ATPase activity drives PspA membrane constriction Authors: Junglas, B. / Hudina, E. / Schoennenbeck, P. / Ritter, I. / Santiago-Schuebel, B. / Huesgen, P. / Sachse, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8aky.cif.gz | 3.7 MB | Display | PDBx/mmCIF format |
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PDB format | pdb8aky.ent.gz | Display | PDB format | |
PDBx/mmJSON format | 8aky.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8aky_validation.pdf.gz | 1.8 MB | Display | wwPDB validaton report |
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Full document | 8aky_full_validation.pdf.gz | 1.8 MB | Display | |
Data in XML | 8aky_validation.xml.gz | 253.8 KB | Display | |
Data in CIF | 8aky_validation.cif.gz | 349 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ak/8aky ftp://data.pdbj.org/pub/pdb/validation_reports/ak/8aky | HTTPS FTP |
-Related structure data
Related structure data | 15497MC 8akqC 8akrC 8aksC 8aktC 8akuC 8akvC 8akwC 8akxC 8akzC 8al0C M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 28097.758 Da / Num. of mol.: 60 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Synechocystis sp. PCC 6803 (bacteria) / Strain: PCC 6803 / Kazusa / Gene: im30 / Plasmid: pET50del Details (production host): Based on pET50b(+) with deletion of NusA tag Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): C41 / References: UniProt: P74717 |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: HELICAL ARRAY / 3D reconstruction method: helical reconstruction |
-Sample preparation
Component | Name: Phage shock protein A (PspA) / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Molecular weight | Value: 149.2 kDa/nm / Experimental value: NO |
Source (natural) | Organism: Synechocystis sp. PCC 6803 (bacteria) |
Source (recombinant) | Organism: Escherichia coli BL21(DE3) (bacteria) / Plasmid: pET50del |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Talos Arctica / Image courtesy: FEI Company |
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Microscopy | Model: FEI TALOS ARCTICA |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 4000 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 58 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-Processing
Software | Name: UCSF ChimeraX / Version: 1.3/v9 / Classification: model building / URL: https://www.rbvi.ucsf.edu/chimerax/ / Os: macOS / Type: package |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
Helical symmerty | Angular rotation/subunit: 21.2 ° / Axial rise/subunit: 6.92 Å / Axial symmetry: C4 |
3D reconstruction | Resolution: 4.7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 44377 / Symmetry type: HELICAL |