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- PDB-8agc: Structure of yeast oligosaccharylransferase complex with lipid-li... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8agc | |||||||||
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Title | Structure of yeast oligosaccharylransferase complex with lipid-linked oligosaccharide and non-acceptor peptide bound | |||||||||
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![]() | TRANSFERASE / N-glycosylation OST complex | |||||||||
Function / homology | ![]() oligosaccharyltransferase I complex / oligosaccharyltransferase complex / dolichyl-diphosphooligosaccharide-protein glycotransferase / dolichyl-diphosphooligosaccharide-protein glycotransferase activity / protein N-linked glycosylation via asparagine / protein N-linked glycosylation / protein glycosylation / endoplasmic reticulum membrane / structural molecule activity / membrane / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.1 Å | |||||||||
![]() | Ramirez, A.S. / de Capitani, M. / Pesciullesi, G. / Kowal, J. / Bloch, J.S. / Irobalieva, R.N. / Aebi, M. / Reymond, J.L. / Locher, K.P. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Molecular basis for glycan recognition and reaction priming of eukaryotic oligosaccharyltransferase. Authors: Ana S Ramírez / Mario de Capitani / Giorgio Pesciullesi / Julia Kowal / Joël S Bloch / Rossitza N Irobalieva / Jean-Louis Reymond / Markus Aebi / Kaspar P Locher / ![]() Abstract: Oligosaccharyltransferase (OST) is the central enzyme of N-linked protein glycosylation. It catalyzes the transfer of a pre-assembled glycan, GlcNAcManGlc, from a dolichyl-pyrophosphate donor to ...Oligosaccharyltransferase (OST) is the central enzyme of N-linked protein glycosylation. It catalyzes the transfer of a pre-assembled glycan, GlcNAcManGlc, from a dolichyl-pyrophosphate donor to acceptor sites in secretory proteins in the lumen of the endoplasmic reticulum. Precise recognition of the fully assembled glycan by OST is essential for the subsequent quality control steps of glycoprotein biosynthesis. However, the molecular basis of the OST-donor glycan interaction is unknown. Here we present cryo-EM structures of S. cerevisiae OST in distinct functional states. Our findings reveal that the terminal glucoses (Glc) of a chemo-enzymatically generated donor glycan analog bind to a pocket formed by the non-catalytic subunits WBP1 and OST2. We further find that binding either donor or acceptor substrate leads to distinct primed states of OST, where subsequent binding of the other substrate triggers conformational changes required for catalysis. This alternate priming allows OST to efficiently process closely spaced N-glycosylation sites. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 417 KB | Display | ![]() |
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PDB format | ![]() | 327.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 15420MC ![]() 4161C ![]() 6eznC ![]() 8agbC ![]() 8ageC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
-Protein , 3 types, 3 molecules ABH
#1: Protein | Mass: 81604.539 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: STT3, GI527_G0002399 / Production host: ![]() ![]() References: UniProt: A0A6A5Q0M3, dolichyl-diphosphooligosaccharide-protein glycotransferase |
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#2: Protein | Mass: 6871.818 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: OST4, GI527_G0000704 / Production host: ![]() ![]() |
#8: Protein | Mass: 39518.160 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: OST3, GI527_G0005459 / Production host: ![]() ![]() |
-Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ... , 5 types, 5 molecules CDEFG
#3: Protein | Mass: 9525.090 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 204508 / S288c / Gene: OST5, YGL226C-A, YGL226BC / Production host: ![]() ![]() |
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#4: Protein | Mass: 14712.531 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: OST2, GI527_G0005476 / Production host: ![]() ![]() |
#5: Protein | Mass: 54116.477 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: OST1, GI527_G0003255 / Production host: ![]() ![]() |
#6: Protein | Mass: 31340.443 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: SWP1, GI527_G0004591 / Production host: ![]() ![]() |
#7: Protein | Mass: 49444.438 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: WBP1, GI527_G0001785 / Production host: ![]() ![]() |
-Protein/peptide , 1 types, 1 molecules P
#9: Protein/peptide | Mass: 682.723 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() ![]() |
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-Sugars , 4 types, 6 molecules ![](data/chem/img/NAG.gif)
#10: Polysaccharide | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source | ||||
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#11: Polysaccharide | Source method: isolated from a genetically manipulated source #12: Polysaccharide | alpha-D-glucopyranose-(1-2)-alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose-(1-3)-alpha-D- ...alpha-D-glucopyranose-(1-2)-alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose-(1-3)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose | Type: oligosaccharide / Mass: 1883.668 Da / Num. of mol.: 1 / Source method: obtained synthetically #17: Sugar | |
-Non-polymers , 5 types, 9 molecules ![](data/chem/img/ELU.gif)
![](data/chem/img/CPL.gif)
![](data/chem/img/PTY.gif)
![](data/chem/img/MN.gif)
![](data/chem/img/323.gif)
![](data/chem/img/CPL.gif)
![](data/chem/img/PTY.gif)
![](data/chem/img/MN.gif)
![](data/chem/img/323.gif)
#13: Chemical | ChemComp-ELU / | ||||||
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#14: Chemical | ChemComp-CPL / #15: Chemical | #16: Chemical | ChemComp-MN / | #18: Chemical | ChemComp-323 / | |
-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Yeast OST complex with lipid-linked oligosaccharide and non-acceptor peptide bound Type: COMPLEX / Entity ID: #1-#8 / Source: NATURAL |
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Molecular weight | Value: 0.284 MDa / Experimental value: NO |
Source (natural) | Organism: ![]() ![]() |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE-PROPANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2400 nm / Nominal defocus min: 600 nm |
Image recording | Electron dose: 64 e/Å2 / Film or detector model: GATAN K2 QUANTUM (4k x 4k) |
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Processing
Software | Name: PHENIX / Version: 1.17.1_3660: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 75382 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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