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- PDB-7yml: Structure of photosynthetic LH1-RC super-complex of Rhodobacter c... -
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Basic information
Entry | Database: PDB / ID: 7yml | |||||||||||||||||||||
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Title | Structure of photosynthetic LH1-RC super-complex of Rhodobacter capsulatus | |||||||||||||||||||||
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![]() | PHOTOSYNTHESIS / LH1-RC COMPLEX / PURPLE BACTERIA | |||||||||||||||||||||
Function / homology | ![]() organelle inner membrane / plasma membrane-derived chromatophore membrane / plasma membrane light-harvesting complex / bacteriochlorophyll binding / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / photosynthetic electron transport in photosystem II / membrane / metal ion binding / plasma membrane Similarity search - Function | |||||||||||||||||||||
Biological species | ![]() | |||||||||||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.6 Å | |||||||||||||||||||||
![]() | Tani, K. / Kanno, R. / Ji, X.-C. / Satoh, I. / Kobayashi, Y. / Nagashima, K.V.P. / Hall, M. / Yu, L.-J. / Kimura, Y. / Mizoguchi, A. ...Tani, K. / Kanno, R. / Ji, X.-C. / Satoh, I. / Kobayashi, Y. / Nagashima, K.V.P. / Hall, M. / Yu, L.-J. / Kimura, Y. / Mizoguchi, A. / Humbel, B.M. / Madigan, M.T. / Wang-Otomo, Z.-Y. | |||||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Rhodobacter capsulatus forms a compact crescent-shaped LH1-RC photocomplex. Authors: Kazutoshi Tani / Ryo Kanno / Xuan-Cheng Ji / Itsusei Satoh / Yuki Kobayashi / Malgorzata Hall / Long-Jiang Yu / Yukihiro Kimura / Akira Mizoguchi / Bruno M Humbel / Michael T Madigan / Zheng-Yu Wang-Otomo / ![]() ![]() ![]() Abstract: Rhodobacter (Rba.) capsulatus has been a favored model for studies of all aspects of bacterial photosynthesis. This purple phototroph contains PufX, a polypeptide crucial for dimerization of the ...Rhodobacter (Rba.) capsulatus has been a favored model for studies of all aspects of bacterial photosynthesis. This purple phototroph contains PufX, a polypeptide crucial for dimerization of the light-harvesting 1-reaction center (LH1-RC) complex, but lacks protein-U, a U-shaped polypeptide in the LH1-RC of its close relative Rba. sphaeroides. Here we present a cryo-EM structure of the Rba. capsulatus LH1-RC purified by DEAE chromatography. The crescent-shaped LH1-RC exhibits a compact structure containing only 10 LH1 αβ-subunits. Four αβ-subunits corresponding to those adjacent to protein-U in Rba. sphaeroides were absent. PufX in Rba. capsulatus exhibits a unique conformation in its N-terminus that self-associates with amino acids in its own transmembrane domain and interacts with nearby polypeptides, preventing it from interacting with proteins in other complexes and forming dimeric structures. These features are discussed in relation to the minimal requirements for the formation of LH1-RC monomers and dimers, the spectroscopic behavior of both the LH1 and RC, and the bioenergetics of energy transfer from LH1 to the RC. | |||||||||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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PDBx/mmCIF format | ![]() | 454.7 KB | Display | ![]() |
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PDB format | ![]() | 398.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 3.9 MB | Display | ![]() |
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Full document | ![]() | 4.1 MB | Display | |
Data in XML | ![]() | 104.2 KB | Display | |
Data in CIF | ![]() | 133.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 33931MC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
-Reaction center protein ... , 2 types, 2 molecules LM
#1: Protein | Mass: 31640.924 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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#2: Protein | Mass: 34405.578 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
-Photosynthetic reaction center ... , 2 types, 2 molecules HX
#3: Protein | Mass: 28384.271 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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#6: Protein | Mass: 8550.882 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
-Light-harvesting protein B-870 ... , 2 types, 20 molecules ADFIKOQSVYBEGJNPRTWZ
#4: Protein | Mass: 6628.930 Da / Num. of mol.: 10 / Source method: isolated from a natural source / Source: (natural) ![]() #5: Protein/peptide | Mass: 5470.303 Da / Num. of mol.: 10 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Sugars , 1 types, 18 molecules ![](data/chem/img/LMT.gif)
#13: Sugar | ChemComp-LMT / |
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-Non-polymers , 10 types, 78 molecules ![](data/chem/img/BCL.gif)
![](data/chem/img/BPH.gif)
![](data/chem/img/U10.gif)
![](data/chem/img/PGV.gif)
![](data/chem/img/PEE.gif)
![](data/chem/img/LDA.gif)
![](data/chem/img/FE.gif)
![](data/chem/img/SPO.gif)
![](data/chem/img/MYR.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/BPH.gif)
![](data/chem/img/U10.gif)
![](data/chem/img/PGV.gif)
![](data/chem/img/PEE.gif)
![](data/chem/img/LDA.gif)
![](data/chem/img/FE.gif)
![](data/chem/img/SPO.gif)
![](data/chem/img/MYR.gif)
![](data/chem/img/HOH.gif)
#7: Chemical | ChemComp-BCL / #8: Chemical | #9: Chemical | ChemComp-U10 / #10: Chemical | ChemComp-PGV / ( #11: Chemical | ChemComp-PEE / | #12: Chemical | ChemComp-LDA / #14: Chemical | ChemComp-FE / | #15: Chemical | ChemComp-SPO / #16: Chemical | ChemComp-MYR / | #17: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Photosynthetic LH1-RC complex from the purple phototrophic bacterium Rhodobacter capsulatus Type: COMPLEX / Entity ID: #1-#6 / Source: NATURAL |
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Molecular weight | Experimental value: NO |
Source (natural) | Organism: ![]() |
Buffer solution | pH: 7.5 |
Specimen | Conc.: 5 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES / Details: This sample was monodisperse. |
Vitrification | Instrument: LEICA EM GP / Cryogen name: ETHANE / Humidity: 80 % / Chamber temperature: 277 K |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2800 nm / Nominal defocus min: 700 nm / Alignment procedure: COMA FREE |
Specimen holder | Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Image recording | Average exposure time: 31.8 sec. / Electron dose: 40 e/Å2 / Detector mode: COUNTING / Film or detector model: FEI FALCON III (4k x 4k) |
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Processing
Software | Name: PHENIX / Version: 1.20_4459: / Classification: refinement | ||||||||||||||||||||||||||||||||
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EM software |
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CTF correction | Type: PHASE FLIPPING ONLY | ||||||||||||||||||||||||||||||||
3D reconstruction | Resolution: 2.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 224431 / Symmetry type: POINT | ||||||||||||||||||||||||||||||||
Atomic model building | B value: 50 / Protocol: RIGID BODY FIT / Space: REAL / Target criteria: Correlation coefficient | ||||||||||||||||||||||||||||||||
Atomic model building | PDB-ID: 7F0L | ||||||||||||||||||||||||||||||||
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