+Open data
-Basic information
Entry | Database: PDB / ID: 7u6o | |||||||||
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Title | Glutamine Synthetase Type III from Ostreococcus tauri | |||||||||
Components | Glutamine synthetase | |||||||||
Keywords | LIGASE / hexamer / glutamine | |||||||||
Function / homology | Function and homology information | |||||||||
Biological species | Ostreococcus tauri (plant) | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.2 Å | |||||||||
Authors | Novikova, I.V. / Powell, S.M. / Evans, J.E. | |||||||||
Funding support | United States, 2items
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Citation | Journal: To Be Published Title: Eukaryotic Glutamine Synthetase Type III Assembles as a Hexamer and Lacks Ring-Ring Quaternary Contacts. Authors: Novikova, I.V. / Powell, S.M. / Smallwood, C.R. / Purvine, S.O. / Zhou, M. / Evans, J.E. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7u6o.cif.gz | 633.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7u6o.ent.gz | 530 KB | Display | PDB format |
PDBx/mmJSON format | 7u6o.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7u6o_validation.pdf.gz | 1.2 MB | Display | wwPDB validaton report |
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Full document | 7u6o_full_validation.pdf.gz | 1.3 MB | Display | |
Data in XML | 7u6o_validation.xml.gz | 111.7 KB | Display | |
Data in CIF | 7u6o_validation.cif.gz | 166.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/u6/7u6o ftp://data.pdbj.org/pub/pdb/validation_reports/u6/7u6o | HTTPS FTP |
-Related structure data
Related structure data | 26377MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 80098.070 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Ostreococcus tauri (plant) / Gene: BE221DRAFT_61941 / Production host: Triticum aestivum (bread wheat) / References: UniProt: A0A1Y5I5Q4, glutamine synthetase |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: A hexamer complex of eukaryotic glutamine synthetase type III Type: CELL / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: Ostreococcus tauri (plant) |
Source (recombinant) | Organism: Triticum aestivum (bread wheat) |
Buffer solution | pH: 7.5 |
Specimen | Conc.: 1.2 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: TFS KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 5000 nm |
Image recording | Electron dose: 100 e/Å2 / Film or detector model: GATAN K2 BASE (4k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.19.2_4158: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 270133 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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