: / Tyrosine-tRNA ligase, archaeal/eukaryotic-type / Aminoacyl-tRNA synthetase, class Ic / tRNA synthetases class I (W and Y) / Rossmann-like alpha/beta/alpha sandwich fold 類似検索 - ドメイン・相同性
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R35GM118070
米国
National Science Foundation (NSF, United States)
F32GM139385
米国
Howard Hughes Medical Institute (HHMI)
Hanna H. Gray Fellowship
米国
引用
ジャーナル: Science / 年: 2021 タイトル: A viral RNA hijacks host machinery using dynamic conformational changes of a tRNA-like structure. 著者: Steve L Bonilla / Madeline E Sherlock / Andrea MacFadden / Jeffrey S Kieft / 要旨: Viruses require multifunctional structured RNAs to hijack their host’s biochemistry, but their mechanisms can be obscured by the difficulty of solving conformationally dynamic RNA structures. Using ...Viruses require multifunctional structured RNAs to hijack their host’s biochemistry, but their mechanisms can be obscured by the difficulty of solving conformationally dynamic RNA structures. Using cryo–electron microscopy (cryo-EM), we visualized the structure of the mysterious viral transfer RNA (tRNA)–like structure (TLS) from the brome mosaic virus, which affects replication, translation, and genome encapsidation. Structures in isolation and those bound to tyrosyl-tRNA synthetase (TyrRS) show that this ~55-kilodalton purported tRNA mimic undergoes large conformational rearrangements to bind TyrRS in a form that differs substantially from that of tRNA. Our study reveals how viral RNAs can use a combination of static and dynamic RNA structures to bind host machinery through highly noncanonical interactions, and we highlight the utility of cryo-EM for visualizing small, conformationally dynamic structured RNAs.
履歴
登録
2021年9月27日
登録サイト: RCSB / 処理サイト: RCSB
改定 1.0
2021年12月1日
Provider: repository / タイプ: Initial release
改定 1.1
2024年6月5日
Group: Data collection / カテゴリ: chem_comp_atom / chem_comp_bond