+Open data
-Basic information
Entry | Database: PDB / ID: 7qca | ||||||
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Title | Spraguea lophii ribosome | ||||||
Components |
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Keywords | RIBOSOME / Microsporidia | ||||||
Function / homology | Function and homology information 90S preribosome / translation regulator activity / maturation of LSU-rRNA / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein kinase C binding / modification-dependent protein catabolic process / protein tag activity ...90S preribosome / translation regulator activity / maturation of LSU-rRNA / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein kinase C binding / modification-dependent protein catabolic process / protein tag activity / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosome binding / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit / negative regulation of translation / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / ribonucleoprotein complex / positive regulation of protein phosphorylation / translation / mRNA binding / ubiquitin protein ligase binding / nucleolus / RNA binding / zinc ion binding / membrane / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Spraguea lophii 42_110 (fungus) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.79 Å | ||||||
Authors | Gil Diez, P. / McLaren, M. / Isupov, M.N. / Daum, B. / Conners, R. / Williams, B. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: Nat Microbiol / Year: 2023 Title: CryoEM reveals that ribosomes in microsporidian spores are locked in a dimeric hibernating state Authors: McLaren, M. / Conners, R. / Isupov, M.N. / Gil-Diez, P. / Gambelli, L. / Gold, V.A.M. / Walter, A. / Connell, S.R. / Williams, B. / Daum, B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7qca.cif.gz | 4.4 MB | Display | PDBx/mmCIF format |
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PDB format | pdb7qca.ent.gz | Display | PDB format | |
PDBx/mmJSON format | 7qca.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qc/7qca ftp://data.pdbj.org/pub/pdb/validation_reports/qc/7qca | HTTPS FTP |
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-Related structure data
Related structure data | 13892MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
-RNA chain , 3 types, 3 molecules L50L70S60
#1: RNA chain | Mass: 849039.812 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Strain: 42_110 / Production host: Spraguea lophii (fungus) |
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#2: RNA chain | Mass: 38356.801 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Strain: 42_110 / Production host: Spraguea lophii (fungus) |
#41: RNA chain | Mass: 444812.531 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Production host: Spraguea lophii (fungus) |
+60S ribosomal protein ... , 27 types, 27 molecules LA0LB0LC0LCCLD0LDDLE0LEELF0LFFLG0LH0LIILJ0LL0LLLLOOLP0LPPLQ0LR0LS0LT0LU0LX0LY0LZ0
-Protein , 14 types, 14 molecules LAALHHLI0LJJLM0LMMSB0SBBSCCSEESFFSGGSR0SX0
#4: Protein | Mass: 16609.498 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Strain: 42_110 / Production host: Spraguea lophii (fungus) |
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#17: Protein | Mass: 14183.866 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Strain: 42_110 / Gene: SLOPH_2194 / Production host: Spraguea lophii (fungus) |
#18: Protein | Mass: 25142.328 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Strain: 42_110 / Gene: SLOPH_1601 / Production host: Spraguea lophii (fungus) / References: UniProt: S7WBF8 |
#21: Protein | Mass: 10517.657 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Production host: Spraguea lophii (fungus) |
#24: Protein | Mass: 14158.396 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Strain: 42_110 / Gene: SLOPH_1179 / Production host: Spraguea lophii (fungus) / References: UniProt: S7XVN9 |
#25: Protein | Mass: 14563.868 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Strain: 42_110 / Gene: SLOPH_2019 / Production host: Spraguea lophii (fungus) / References: UniProt: S7XSQ3 |
#44: Protein | Mass: 25934.658 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Production host: Spraguea lophii (fungus) |
#45: Protein | Mass: 9189.923 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Production host: Spraguea lophii (fungus) |
#47: Protein | Mass: 7288.479 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Production host: Spraguea lophii (fungus) |
#51: Protein | Mass: 6816.010 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Production host: Spraguea lophii (fungus) |
#53: Protein | Mass: 16937.791 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Strain: 42_110 / Gene: SLOPH_2039 / Production host: Spraguea lophii (fungus) / References: UniProt: S7W9X5 |
#55: Protein | Mass: 36182.824 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Strain: 42_110 / Gene: SLOPH_2232 / Production host: Spraguea lophii (fungus) / References: UniProt: S7XVG3 |
#66: Protein | Mass: 14055.251 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Production host: Spraguea lophii (fungus) |
#72: Protein | Mass: 15822.722 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Production host: Spraguea lophii (fungus) |
-Ribosomal protein ... , 7 types, 7 molecules LGGLN0LO0LV0LW0SP0SV0
#15: Protein | Mass: 12020.348 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Strain: 42_110 / Gene: SLOPH_1567 / Production host: Spraguea lophii (fungus) / References: UniProt: S7W7C6 |
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#26: Protein | Mass: 24200.426 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Strain: 42_110 / Gene: SLOPH_1565 / Production host: Spraguea lophii (fungus) / References: UniProt: S7W7A2 |
#27: Protein | Mass: 22885.139 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Strain: 42_110 / Gene: SLOPH_1564 / Production host: Spraguea lophii (fungus) / References: UniProt: S7XUD8 |
#36: Protein | Mass: 15239.987 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Strain: 42_110 / Gene: SLOPH_2233 / Production host: Spraguea lophii (fungus) / References: UniProt: S7XLC4 |
#37: Protein | Mass: 15342.161 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Strain: 42_110 / Gene: SLOPH_1566 / Production host: Spraguea lophii (fungus) / References: UniProt: S7XSY2 |
#64: Protein | Mass: 18514.629 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Strain: 42_110 / Gene: SLOPH_1570 / Production host: Spraguea lophii (fungus) / References: UniProt: S7XKY9 |
#70: Protein | Mass: 7768.887 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spraguea lophii 42_110 (fungus) / Strain: 42_110 / Gene: SLOPH_2726 / Production host: Spraguea lophii (fungus) / References: UniProt: S7WAC1 |
+40S ribosomal protein ... , 23 types, 23 molecules SA0SAASC0SD0SDDSE0SF0SG0SH0SI0SJ0SK0SL0SM0SN0SO0SQ0SS0ST0SU0SW0SY0SZ0
-Non-polymers , 3 types, 368 molecules
#75: Chemical | ChemComp-K / #76: Chemical | ChemComp-MG / #77: Chemical | ChemComp-ZN / |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Ribosome / Type: RIBOSOME / Entity ID: #1-#74 / Source: NATURAL | |||||||||||||||||||||||||
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Source (natural) | Organism: Spraguea lophii 42_110 (fungus) | |||||||||||||||||||||||||
Buffer solution | pH: 7.5 Details: Solution with HEPES, Potassium Acetate and Magnesium acetate was autoclaved. DTT was added fresh just before sample preparation. | |||||||||||||||||||||||||
Buffer component |
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Specimen | Conc.: 10 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES / Details: Monomeric ribosomes from Spraguea lophii | |||||||||||||||||||||||||
Specimen support | Details: 20 mA. Carbon coated grid / Grid material: COPPER / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil | |||||||||||||||||||||||||
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 288.15 K / Details: blot force -1 and blot time 4 s |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company | ||||||||||||
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EM imaging | Electron source: FIELD EMISSION GUN / Illumination mode: FLOOD BEAM / Mode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 1200 nm / Specimen-ID: 1
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Image recording |
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-Processing
Software | Name: REFMAC / Version: 5.8.0267 / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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EM software |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Particle selection | Num. of particles selected: 977879 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Point symmetry: C1 (asymmetric) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3D reconstruction | Resolution: 2.79 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 285940 / Algorithm: FOURIER SPACE / Symmetry type: POINT | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Atomic model building | Protocol: AB INITIO MODEL / Space: RECIPROCAL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Atomic model building | PDB-ID: 6RM3 Accession code: 6RM3 / Source name: PDB / Type: experimental model | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | Resolution: 2.79→2.79 Å / Cor.coef. Fo:Fc: 0.877 / WRfactor Rwork: 0.256 / SU B: 9.074 / SU ML: 0.169 / Average fsc overall: 0.8886 / Average fsc work: 0.8886 / ESU R: 0.168 / Details: Hydrogens have not been used
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Solvent computation | Solvent model: BABINET MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 79.767 Å2
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Refine LS restraints |
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LS refinement shell | Refine-ID: ELECTRON MICROSCOPY / Num. reflection Rfree: _ / Total num. of bins used: 20 / % reflection obs: 100 %
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