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- EMDB-13892: Spraguea lophii ribosome -

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Basic information

Entry
Database: EMDB / ID: EMD-13892
TitleSpraguea lophii ribosome
Map dataBest overall map for both LSU and SSU
Sample
  • Complex: Ribosome
    • RNA: x 3 types
    • Protein or peptide: x 71 types
  • Ligand: x 3 types
KeywordsMicrosporidia / RIBOSOME
Function / homology
Function and homology information


translation regulator activity / cytosolic ribosome / ribosome binding / large ribosomal subunit / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / rRNA binding / ribosome / structural constituent of ribosome ...translation regulator activity / cytosolic ribosome / ribosome binding / large ribosomal subunit / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / RNA binding / zinc ion binding / membrane / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S26e / Ribosomal protein S26e superfamily / S27a-like superfamily / Ribosomal protein S26e / Ribosomal protein S25 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S21e ...Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S26e / Ribosomal protein S26e superfamily / S27a-like superfamily / Ribosomal protein S26e / Ribosomal protein S25 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / S25 ribosomal protein / Ribosomal protein L10e, conserved site / Ribosomal protein L10e / Ribosomal protein S19A/S15e / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / 40S ribosomal protein S11, N-terminal / Ribosomal protein S7e / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein L34e, conserved site / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein L44e / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal protein S19e / Ribosomal L22e protein family / 40S Ribosomal protein S10 / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal / Ribosomal_S17 N-terminal / Plectin/S10, N-terminal / Ribosomal protein L44 / Ribosomal protein L34Ae / Ribosomal protein S7e / Plectin/S10 domain / Ribosomal protein S8e / Ribosomal L27e protein family / Ribosomal protein S4, KOW domain / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal protein S13/S15, N-terminal / Ribosomal protein S15P / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein L30/YlxQ / Ribosomal protein L13e / Ribosomal protein L13e / RS4NT (NUC023) domain / Ribosomal protein L34e / 60S ribosomal protein L6E / Ribosomal protein L35A / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal L40e family / Ribosomal protein L36e / Ribosomal protein L35A superfamily / Ribosomal protein L44e signature. / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal family S4e / Ribosomal protein L7A/L8 / Ribosomal S13/S15 N-terminal domain / Ribosomal protein L10e signature. / Ribosomal protein L32e, conserved site / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L35Ae / Ribosomal protein S4e signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L34e signature. / Ribosomal protein L31e / Ribosomal protein L31e domain superfamily / Ribosomal_L31e / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein 60S L18 and 50S L18e / 60S ribosomal protein L19 / Ribosomal protein L3, domain 3, archaeal type superfamily / Ribosomal protein L3, archaeal/eukaryotic type / Ribosomal protein L21e / Ribosomal_L15e / Ribosomal protein L15e / Ribosomal protein L15e core domain superfamily / Ribosomal protein L32e / Ribosomal protein L32e superfamily / Ribosomal_L32e
Similarity search - Domain/homology
40S ribosomal protein S5 / 60S ribosomal protein L6 / 60S ribosomal protein L35a / 40S ribosomal protein S14 / Ribosomal protein L15 / Ribosomal protein L34 / 40S ribosomal protein S12 / 40S Ribosomal protein S19 / 40S ribosomal protein S2 / 60S ribosomal protein L26 ...40S ribosomal protein S5 / 60S ribosomal protein L6 / 60S ribosomal protein L35a / 40S ribosomal protein S14 / Ribosomal protein L15 / Ribosomal protein L34 / 40S ribosomal protein S12 / 40S Ribosomal protein S19 / 40S ribosomal protein S2 / 60S ribosomal protein L26 / 40S ribosomal protein S9 / 60s ribosomal protein L21 / 40s ribosomal protein s24 / 40S ribosomal protein S11 / 40S ribosomal protein S0 / 60S ribosomal protein L7 / Large ribosomal subunit protein eL22 / 40S ribosomal protein S20 / 40S ribosomal protein S26 / Ubiquitin/40s ribosomal protein S27a fusion / Ribosomal protein S21E / 60S ribosomal protein L20 / 60S ribosomal protein L18 / 40S ribosomal protein S25 / 40S ribosomal protein S3 / 60S ribosomal protein L3 / 60S ribosomal protein L11 / S60 ribosomal protein L10 / 60S ribosomal protein L19 / 60S ribosomal protein L4 / 60S ribosomal protein L17 / 40S ribosomal protein S16 / 60S ribosomal protein L36 / 60S ribosomal protein L3 / 60S ribosomal protein L23a / 40S ribosomal protein S4 / 40S ribosomal protein S8 / 60S ribosomal protein L5 / 40S ribosomal protein S18 / 40S ribosomal protein S29 / 40S ribosomal protein S15A / 60S ribosomal protein L32 / Ribosomal protein S19 / 60S ribosomal protein L44 / Ribosomal protein L23 / 60S ribosomal protein L9 / Ubiquitin / Ribosomal protein L24E / 60S ribosomal protein L39 / 60S ribosomal protein L27 / 40S ribosomal protein S10 / 60S ribosomal protein L8 / Ribosomal protein L13A / 40S ribosomal protein S7 / Guanine nucleotide binding protein beta subunit / 60S ribosomal protein L13 / Uncharacterized protein / 40S ribosomal protein S13 / 60S ribosomal protein L31
Similarity search - Component
Biological speciesSpraguea lophii 42_110 (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.79 Å
AuthorsGil Diez P / McLaren M
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Wellcome Trust212439/Z/18/Z United Kingdom
CitationJournal: Nat Microbiol / Year: 2023
Title: CryoEM reveals that ribosomes in microsporidian spores are locked in a dimeric hibernating state
Authors: McLaren M / Conners R / Isupov MN / Gil-Diez P / Gambelli L / Gold VAM / Walter A / Connell SR / Williams B / Daum B
History
DepositionNov 22, 2021-
Header (metadata) releaseNov 30, 2022-
Map releaseNov 30, 2022-
UpdateDec 20, 2023-
Current statusDec 20, 2023Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_13892.png

Downloads & links

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Map

FileDownload / File: emd_13892.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationBest overall map for both LSU and SSU
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.05 Å/pix.
x 320 pix.
= 337.28 Å		1.05 Å/pix.
x 320 pix.
= 337.28 Å		1.05 Å/pix.
x 320 pix.
= 337.28 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.054 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.05356
Minimum - Maximum-0.06865198 - 2.4027605
Average (Standard dev.)0.008050524 (±0.061276507)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 337.28 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Overall map before postprocessing

Fileemd_13892_additional_1.map
AnnotationOverall map before postprocessing
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: The map after deepenhancer with masked LSU

Fileemd_13892_additional_2.map
AnnotationThe map after deepenhancer with masked LSU
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: The map after deepenhancer with masked SSU body

Fileemd_13892_additional_3.map
AnnotationThe map after deepenhancer with masked SSU body
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: The map after deepenhancer with masked SSU head

Fileemd_13892_additional_4.map
AnnotationThe map after deepenhancer with masked SSU head
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: The map processed with the mask centered at L1 stalk region

Fileemd_13892_additional_5.map
AnnotationThe map processed with the mask centered at L1 stalk region
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Ribosome

EntireName: Ribosome
Components
  • Complex: Ribosome
    • RNA: RNA 23S
    • RNA: RNA 5S
    • Protein or peptide: 60S ribosomal protein L8
    • Protein or peptide: uL15 LAA
    • Protein or peptide: 60S ribosomal protein L3
    • Protein or peptide: 60S ribosomal protein L4
    • Protein or peptide: 60S ribosomal protein L3
    • Protein or peptide: 60S ribosomal protein L5
    • Protein or peptide: 60S ribosomal protein L31
    • Protein or peptide: 60S ribosomal protein L6
    • Protein or peptide: 60S ribosomal protein L32
    • Protein or peptide: 60S ribosomal protein L7
    • Protein or peptide: 60S ribosomal protein L35a
    • Protein or peptide: 60S ribosomal protein L8
    • Protein or peptide: Ribosomal protein L34
    • Protein or peptide: 60S ribosomal protein L9
    • Protein or peptide: Ribosomal L29 protein
    • Protein or peptide: S60 ribosomal protein L10
    • Protein or peptide: 60S ribosomal protein L36
    • Protein or peptide: 60S ribosomal protein L11
    • Protein or peptide: eL37 LJJ
    • Protein or peptide: 60S ribosomal protein L13
    • Protein or peptide: 60S ribosomal protein L39
    • Protein or peptide: eL14 LM0
    • Protein or peptide: Ubiquitin
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: Ribosomal protein L13ARibosome
    • Protein or peptide: 60S ribosomal protein L44
    • Protein or peptide: 60S ribosomal protein L17
    • Protein or peptide: 60S ribosomal protein L37a
    • Protein or peptide: 60S ribosomal protein L18
    • Protein or peptide: 60S ribosomal protein L19
    • Protein or peptide: 60S ribosomal protein L20
    • Protein or peptide: 60s ribosomal protein L21
    • Protein or peptide: 60S ribosomal protein L22
    • Protein or peptide: Ribosomal protein L23
    • Protein or peptide: Ribosomal protein L24ERibosome
    • Protein or peptide: 60S ribosomal protein L23a
    • Protein or peptide: 60S ribosomal protein L26
    • Protein or peptide: 60S ribosomal protein L27
    • RNA: RNA SSU
    • Protein or peptide: 40S ribosomal protein S0
    • Protein or peptide: 40S ribosomal protein S26
    • Protein or peptide: eS1 SB0
    • Protein or peptide: eS27 SBB
    • Protein or peptide: 40S ribosomal protein S2
    • Protein or peptide: eS28 SCC
    • Protein or peptide: 40S ribosomal protein S3
    • Protein or peptide: 40S ribosomal protein S29
    • Protein or peptide: 40S ribosomal protein S4
    • Protein or peptide: eS30 SEE
    • Protein or peptide: 40S ribosomal protein S5
    • Protein or peptide: Ubiquitin/40s ribosomal protein S27a fusion
    • Protein or peptide: 40S ribosomal protein S6
    • Protein or peptide: Guanine nucleotide binding protein beta subunit
    • Protein or peptide: 40S ribosomal protein S7
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: 40S ribosomal protein S9
    • Protein or peptide: 40S ribosomal protein S10
    • Protein or peptide: 40S ribosomal protein S11
    • Protein or peptide: 40S ribosomal protein S12
    • Protein or peptide: 40S ribosomal protein S13
    • Protein or peptide: 40S ribosomal protein S14
    • Protein or peptide: Ribosomal protein S1940S ribosomal protein S19
    • Protein or peptide: 40S ribosomal protein S16
    • Protein or peptide: eS17 SR0
    • Protein or peptide: 40S ribosomal protein S18
    • Protein or peptide: 40S Ribosomal protein S19
    • Protein or peptide: 40S ribosomal protein S20
    • Protein or peptide: Ribosomal protein S21ERibosome
    • Protein or peptide: 40S ribosomal protein S15A
    • Protein or peptide: uS12 SX0
    • Protein or peptide: 40s ribosomal protein s24
    • Protein or peptide: 40S ribosomal protein S25
  • Ligand: POTASSIUM IONPotassium
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: Ribosome

SupramoleculeName: Ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#74
Source (natural)Organism: Spraguea lophii 42_110 (fungus)

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Macromolecule #1: RNA 23S

MacromoleculeName: RNA 23S / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 849.039812 KDa
SequenceString: AUACCACACA CAAGGGAUCG UUUGGCUCCU GGGACGAGGA AGGGCGCAGC AGAGAGCGAU AUGUGUGGGA GCAGCAGCAA UGACCACAC GUCCCCGAAU ACAGCAUAGU GCUGGAGUAC UCCUUGGAAU UAAGCAUAUG AGUAAAGGAA GGAAAAGAAA C UAACGAGG ...String:
AUACCACACA CAAGGGAUCG UUUGGCUCCU GGGACGAGGA AGGGCGCAGC AGAGAGCGAU AUGUGUGGGA GCAGCAGCAA UGACCACAC GUCCCCGAAU ACAGCAUAGU GCUGGAGUAC UCCUUGGAAU UAAGCAUAUG AGUAAAGGAA GGAAAAGAAA C UAACGAGG AUUCCCGUAG UAGCGGCGAG UGAACAGGGA ACAGUCCAAC AGUGUAAUUC UCAAAUGAGA AGUUGUCAAA AG GAAGAAG AUGUGAAUCA GAAAGAAAAG CUGAACGAUA CAGGGUGAUA GUCCCGUAGU GUCUUCUUAC AUACGGUGAG UAG CUGUGC UCGGUAAUGC ACAGUGAAGA GGUGGCAGUG UGCAUCUAAG ACUAAAUACA ACAGGAGACC GAUAGCAAAG AAGU AGGUU GACCGAAAAC AUCUAAGUGA AAUUGUGUGU GGACCCGAAU GGAUCGGGCC CGUCUUGAAA CACGGACCAA GGAGU GCAC AUACACUGCA AGUUGAGCAG AGGAAGUCUG UGAGGCGUAG UGAAGACGGA AAGCAGUGUG AGUGCGACCC GAUAGA CUU GUGAACUAUA CCUUGUUGCU GUGAAGGCUG GCGAAAGCCA GCUGGAGGCG GCAAGCCGUA UUGAUCUGCA AAUCAUU GG CGUAAGCAGG GUAUAGGGGC GAAAGACCAA UCGAACAGUC UAGUUGCUGG UUCCCUCCGA AAUGUCUAUC AGGACAGC G CGCACGCAGA GAGAAGCAAG GUAGAGCAUG GGCCGGUAUC CUUAAGGAGA CGGACGAACU GCGAAUGUGC UUUUCUUCU AUGCAAGCGU AGUGGGCGAG UUCUGGUAAG CAGGACUGGC GAAGAGGAAU GAACCUGGCA CUGUGCUAAG GAACCCAAUG UCUGGACAG AGACCGAAAG AGGUAGGUGA AUAAGGACGG UAGGGCGGUA GCCAUGGAAG UCGGCAUCCG GUAAGAAACG U GUUACAAC GUACCUACGG AAUUCAUCUG CUCUGAAAAU UGAUGGCGCU UACCAGACAU CCGAUGCACA GUUAGCAGGU AG GAGGGCG UGUGUCUACU GGCGAAGGUA CCGAGUGAUC GGUGGUGGAG GUAGAUGCAG AGCAGAUCUU GGUGGUAGUA GCG AUAAUU UGUCUUCAGC GGCAAAGACU GAGGAGGAGA AGGGUUUCUU CUCAUAAGAA GAGUGAGCCG GGUCUAAGCG AGUG UGUAG AAGCAUGAGC GAAGGAGAAA CAGGUUGAUA UUCCUGUGCC UGUGCUGUCG UACGGCAACG UGUAUUAGCU UGUCG ACAC GAACAUCUAG ACAAAGCAGA AACACCUUUU CUGUUCAAGG GGAAAAAGAC CACUGAGACG GAUCAUCUGG CGACGU GGU UUUAUAUCUG UGACCAGGCC UGAGCCUGGU UUGUUGAGGA UGGUCGUCCA UGAAAAGACA ACUAUAUGGC AGCACAG UC CGUACCAACC GUAUCAGGAC UCCAAGGUGA AGAGCCUCUA GUGGAUGUUU UACACAUGUA AGGGAAUUCG GCAAAAUG G AUGCGUAACU UCGGGAGAAG UAUUGGUUCA AUUGUGAACU CAUUGUGACA AGGGGAAUCU GACUGUUUAG UAAAAACAU AGCUUCAUGA AGAGAGAUGA AGUGAGUUCU GCCCGGUGCU CGGUCGUGAC ACGGAGGAAA UGCCAGAAAG CACGGGUUAA CGGCGGGAG UAACUAUGAC UCUCUUAAGG UAGCCAAACG CCUCGUCAUU UAAAUGGUGA CGCGCAUGAA UGGAAUGACG A GAUUCCCA CUGUCCCUAC AUGUAGACUA GCGAACCCAU UUCCAGGGGA ACGGGCCUGG ACGGCCAGCG GGGAAAGAAG AC CCUGUUG AGCUUGACUC UAGUGUGGCC AAGUUGUGAC GAUUGAGGCG AUGUAGGAAG GUGGAGAGCG CAAGCAGAAG UGA AAGACC ACUGCGCCAU GAUCAUCACG ACACACUUGU UUAAGGACAA AGGCCAGAUG GGGAGUUUGG CUGGGGCGGU ACGA CCACA GAAUCAGAAC GUGGUCGACC GAAGGUACUC ACAGCGAGAC GAGAACACUC GUGUAGAGCA UAAGGACUAA AGAGU GCCU GAGUGUGAGC ACUACUGCUU GCAUGAGGGG AAACCCGGGC CUAGCGAUCC UACGCAUACG AGACACUUGU GGCGUG GGU GUCAGAAAAG UUACCACAGG GAUAACUGGC UUGUGGCCGC CGAGCGUAGA AAGCGACGCG GCUUUUUGAU UCUUCGA UG UCGGCUCUUC CUAGCAUGGC CAUGCAGCCG UGGCGAAGUG UUGGAUUGUU CACCCACUAA CAGGGAACGU GAGCUGGG U UUAGACCGUC GUGAGACAGG UUAGUUUUAU CCUACUGUCC AAACAGUUGA AGGGAGUGUG GAUUAGUACG AAAGGAAAC CCACACGUGA CCUCUGGUGC AGCGGGUGUG CGGAAGUGCA ACUGCCAUGC UACGUCUCCU CAAGCAUAGC UGGAAGCCUC UAAGCUAGA AAUGAGUCCU GACUCUGGAA GACGGCCGGG AAGACGACCC GUAGUUAACA GCGGUGUUGU ACGAUAUGAG C UUUCUAUU UGUGUAGUAG CGAGUAUUUG AGACACUGGU UGUUACCACC GUCGUUUAUU

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Macromolecule #2: RNA 5S

MacromoleculeName: RNA 5S / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 38.356801 KDa
SequenceString:
AGUUACGGCC AUAUCUACUG AAAAACACCG GAUCCCGUCC GAUCUCCGAA GUUAAGCCAA UAAGAGCCAU GCGAGUAUUA AGGUGGGCG ACUACUUGAG AAAGCGUGGU GCUGUAGUUU

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Macromolecule #41: RNA SSU

MacromoleculeName: RNA SSU / type: rna / ID: 41 / Number of copies: 1
Source (natural)Organism: Spraguea lophii 42_110 (fungus)
Molecular weightTheoretical: 444.812531 KDa
SequenceString: ACCAGGUUGA UUCUGCCUGA CGUGGAUGCU AGUCUCAUAG AUUUAGCCAU GCAUGUGUAA GCGAAGCGAU UAGUGAAGCG GCGGAAGGC UCAGUAACGG GCGAGUAUUU AAUCUCUAGA ACCGGACAAC CUCAGGAAAC AGAGGGGAAA ACGGAGUAAG A GCAUGUGU ...String:
ACCAGGUUGA UUCUGCCUGA CGUGGAUGCU AGUCUCAUAG AUUUAGCCAU GCAUGUGUAA GCGAAGCGAU UAGUGAAGCG GCGGAAGGC UCAGUAACGG GCGAGUAUUU AAUCUCUAGA ACCGGACAAC CUCAGGAAAC AGAGGGGAAA ACGGAGUAAG A GCAUGUGU UGUUAGCAUG UGAUGAGAAC AACAGUGCAG GAGAGUAAGA AGCCAUCCCA UCAGUUAGUA AGUAGGGUAA GG GCCUAUU UAGACGUAGA CGGGUACGGG GGAUUAGGGU UUGAUUCCGG AGAGGGAGCC UGAGAGACGG CUACCAGGUC CAA GGACAG CAGCAGGCGC GAAAAUUACC GAAGCCUCCA AGAGGGCGGU AGUGAAGAGA CGUGAAAACA AGUGUGUGUA AAAA CCACA UGAGUAACUG GAGGUCAAGG CUGGUGCCAG CAUCCGCGGU AAUACCAGCU CCAGGAGUGU CUAUGAUGAU UGCUG CGAU UAAAAGGUCC GUAGUCGAAG AACAGUACUU GUUUGUAAUG UGGCUUAUCG AGAGCCGAAU AGAACGAGAA GAGUUC AGA GUAAGGAGCG AAGGAGGGCU AGAUUAUUGA GCAGCGAGAG GUGAAAUUUG AUGACCUGCU UAGGAGUAAC AGAGGCG AA AGCGCUAGUC AAGAACGAAU CCGAUGAUCA AGGACGUAGG CUGGAGGAUC GAAGACGAUU AGAGACCGUA GUAGUUCC A GCAGUAAACG AUGCCUACGC UGUUGUGGUU AUGAGCACGG CAGAAGAGAA AUCUAGUAGG GCUUUGGGGA GAGUACGCG CGCAAGCGAU AAAUUUAAAG GAAAUUGACG GAAGAACACC ACAAGGAGUG GAGUGUGCGG CUUAAUUUGA CUCAACGCGG GACAGCUUA CCAUACCCGA UGGCCGUGUG AGCGUAGUAC GCGAUAGGUC AGAGAGUGGU GCAUGGCCGU UAACGACGAG U GGGGUGAC CUUUGGGUUA AGUCCGCGAA GUAGUGAGAU CCCUAGGAUA CGAAAGUUAU CGACAGGUGU UAAAAACACA GG AAGGAAG GGGCAAGAAC AGGUCAGUGA UGCCCUUAGA UGGUAUGGGC UGCACGCGCA CUACAGUGGU CACAGCAGAA ACA AAUAGA AGUAAAUGUG AUCGAGAGGG AAUGAGCACU GUAAUGUGCA CAGGAACGAG GAAUUGCUAG UAAUCGUUAU CUCA UUAAG AAGCGAUGAA UGUGUCCCUG UUCUUUGUAC ACACCGCCCG UCGUUAUCGA AGAUGGAACC AGGUGCGAAC AAGUG AAAG CGAGUGAGUG CAGGGUUCUA GAUCUGAUAC AAGUCGUAAC AAGGCAGCUG UAGGAGAACC UGUAGCUGGA UCAACU AGU UAAUU

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Macromolecule #3: 60S ribosomal protein L8

MacromoleculeName: 60S ribosomal protein L8 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 27.0055 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MGKVIRTLRL QKEHWKRKHH KSKGIVGFPT LKNCEATVEK IVHESGRPAP LAIINYKKEE KNTKGLLVAI EGIYEGQKIQ IGENASLEL GNVCPIKEIP EGSLVSMVEK KPKDGGKYVK SPGCYATIVY HNKLTNQTTI KLPSGERVRV TGESRALLGI I AGGGKDDK ...String:
MGKVIRTLRL QKEHWKRKHH KSKGIVGFPT LKNCEATVEK IVHESGRPAP LAIINYKKEE KNTKGLLVAI EGIYEGQKIQ IGENASLEL GNVCPIKEIP EGSLVSMVEK KPKDGGKYVK SPGCYATIVY HNKLTNQTTI KLPSGERVRV TGESRALLGI I AGGGKDDK PLLKAVNAYY KYKTKGKPWP RVRGVAMNPV DHPHGGGNHQ HVGHPTTVSR NAPVNGRVGL VAARRTGRKK GS RKITVN

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Macromolecule #4: uL15 LAA

MacromoleculeName: uL15 LAA / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 16.609498 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MFNKKSKSRK LRGHVSHGYG RVGKHRKHPA GRGKSGGLKH LRSLFQRYHP DHFRKLGIVM FHRNKNADFT RTVNVSNLWG LMKLEEQMK FKSSAEVPVV DCRNYGYLKV LGGGDLSVKK PIVVIARQFT KDAEEKINAV GGKCVVAA

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Macromolecule #5: 60S ribosomal protein L3

MacromoleculeName: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 44.320641 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MSCRNYSAPR HGSLAYCPKK RAKTTKPAIK SFPKDDASKP VHLTGYLAYK VGMTHVVRLS TRRSKTERVL KETQKEVLDA VTILEVPAM RIFGMVGYKS TVNGLKAIKT VYAEHLDEQV LARLRKRNSR SSKLMFKLYP QKYENGDIKR DYELLKRESD V VRVLAHTQ ...String:
MSCRNYSAPR HGSLAYCPKK RAKTTKPAIK SFPKDDASKP VHLTGYLAYK VGMTHVVRLS TRRSKTERVL KETQKEVLDA VTILEVPAM RIFGMVGYKS TVNGLKAIKT VYAEHLDEQV LARLRKRNSR SSKLMFKLYP QKYENGDIKR DYELLKRESD V VRVLAHTQ VSKIPSLNLK KAHILEIQLN GGTIEEKIEW AIERLEKEIP VSEVFSQQDL IDVIGVTKGK GKEGTTKRFG TT ILQHKSR KGKRKVACIG AWHPANVRRT VARAGQLGFH RRTAQNKKIY MMGNGKTPFS TEYDLTSKTI NPMGGFLRYG NVK SDFIMV KGGIQGPSKR VLALRKAFAP SNLGKDNEEI VLKFVDASSK KGYGRFQTTE EKKAYFGIKK AEVVEQ

UniProtKB: 60S ribosomal protein L3

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Macromolecule #6: 60S ribosomal protein L4

MacromoleculeName: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 37.241633 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MSRQVNIIEQ DGTSSRTTSV PAVFNLNVRQ DLISFTHKNV AMNSRQPYAV KPGAGMRHSA KSWGTGRAMA RVPRVRAGGT RRAGQGANA NFCRGGRMFA PTNVNRRWNR KTLLSIRRYA SAMAVAATSI PAYVEGRGHR IEQIENIPLV LSNKIDTEKT K EGIKILYN ...String:
MSRQVNIIEQ DGTSSRTTSV PAVFNLNVRQ DLISFTHKNV AMNSRQPYAV KPGAGMRHSA KSWGTGRAMA RVPRVRAGGT RRAGQGANA NFCRGGRMFA PTNVNRRWNR KTLLSIRRYA SAMAVAATSI PAYVEGRGHR IEQIENIPLV LSNKIDTEKT K EGIKILYN LGLKEELDKV EESKNIRAGK GKWRNRRYVL KKGPLFVYTS ETSFTKAIRN IPGVDTERVE NLSILDLAPG GQ AGRLIIW MEDAFEKLTE IYGEMNEESK FKKGYTMGMD VAENIDVENI FYSDEVQEFI DIPNFIKKDK MVMEKNKLAE IQQ YVEIEE RI

UniProtKB: 60S ribosomal protein L4

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Macromolecule #7: 60S ribosomal protein L3

MacromoleculeName: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 12.484438 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MSRKRSKREE SFVDKLPLAL KTGKVNLGFK ESLKSLVEKK TKLLILTSNF SPIKRKTLEY YAAISNNTPI YYYDGSNNDL SKNCGKYFR IGVISIQDFG EADLMNAMAQ A

UniProtKB: 60S ribosomal protein L3

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Macromolecule #8: 60S ribosomal protein L5

MacromoleculeName: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 33.89225 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MVSNSINFQS DNKSKKSYFQ RFQTKKRRRR LCKTNYRRRT KLIKLDQKNV GEDKSRLVVR ITNSKVICQV VKSHLGGDMV VAQATSAEL KEFGLKVGFT NYSAAYATGL LCARKILRME KLDNIYKPKF CTDVDVVEDI EGEKNAYRCY LDIGLSRNSK G AKVFAAMK ...String:
MVSNSINFQS DNKSKKSYFQ RFQTKKRRRR LCKTNYRRRT KLIKLDQKNV GEDKSRLVVR ITNSKVICQV VKSHLGGDMV VAQATSAEL KEFGLKVGFT NYSAAYATGL LCARKILRME KLDNIYKPKF CTDVDVVEDI EGEKNAYRCY LDIGLSRNSK G AKVFAAMK GASDGGIKIP YSPKIFPGYN KDEEFDQQKL RDRIFGKEVA NYMQLLKKED ETKFKKQFSE YIKQNINAED LE KMYENLF NSIVTKEISK KEKKDYSALK KYKVMKLTKE ERDARVQQKL VENN

UniProtKB: 60S ribosomal protein L5

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Macromolecule #9: 60S ribosomal protein L31

MacromoleculeName: 60S ribosomal protein L31 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 12.854213 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MAFKDDKAIE LTIPMGKITI DVSKRWKCRI GIRRLKKFIT KTFHDKEAEV QISPDLNKFL WERGMRNVPK RVRVRVNQEP YPKDPSKKV YKLSHVVVST FKGLGTEAIA E

UniProtKB: 60S ribosomal protein L31

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Macromolecule #10: 60S ribosomal protein L6

MacromoleculeName: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 20.220115 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MQSERQQRLN ISNIKNYYTA DDIPAFAEKL LELHKPAPIE LRTDLVQGNV VVVLEGEYAS YRVVYLSRTE DNKALCMGLP SINGIGLFE IDERFLLRTS IVLDIRLDKK YRAKESKRSF KKFNTKEKVL TKEENDIEEL LLKEIENEKF MKKYFETPYE I NNTVDFYE INH

UniProtKB: 60S ribosomal protein L6

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Macromolecule #11: 60S ribosomal protein L32

MacromoleculeName: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 16.075998 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MDIVNPSLSK VIDIINKGKS KKQFKRFQSW RVKRVSESWR KPRGIDNRMR RKFGGAPDMP GIGYKKPNAI RFLLPNGYRK IMVACVKDL EALISLNSFY CAEIRHAVGS KKRIEILERA EELGILVINK EGKLVKEEEE

UniProtKB: 60S ribosomal protein L32

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Macromolecule #12: 60S ribosomal protein L7

MacromoleculeName: 60S ribosomal protein L7 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 27.935564 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MSNVDLIAKK EQYNLRMEEL QKKQQEQHLK RVEENKKYAE KRTVELLETY KKIALEQQLS EERMQKENKI YAPKDPHFYA VVLIKSPLR ASNKIKKTLE LFRLKHINNC VLVKNNESSR QMLQKIRNFV AYGELKLKTL RELIYKRGSC HIFSEKLNIT N ETLEDAFK ...String:
MSNVDLIAKK EQYNLRMEEL QKKQQEQHLK RVEENKKYAE KRTVELLETY KKIALEQQLS EERMQKENKI YAPKDPHFYA VVLIKSPLR ASNKIKKTLE LFRLKHINNC VLVKNNESSR QMLQKIRNFV AYGELKLKTL RELIYKRGSC HIFSEKLNIT N ETLEDAFK NIRCIEEFIY ELYFNDERFK ELNNFLNPFK LTPPKGGYKG RKAKDIIEGG AVGNHQDKIG ELIKKMI

UniProtKB: 60S ribosomal protein L7

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Macromolecule #13: 60S ribosomal protein L35a

MacromoleculeName: 60S ribosomal protein L35a / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 12.757991 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MEVIKKIELK ERVCAKGTLY SHKRSKKNIY PRYSLITIEN VKTREAAMKY VGNAVELFKP ESTKEESIHG IIKAVHGRSG AVRAKFSRN ISPVEMGSKV YIKLYKMNED EI

UniProtKB: 60S ribosomal protein L35a

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Macromolecule #14: 60S ribosomal protein L8

MacromoleculeName: 60S ribosomal protein L8 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 23.60499 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MSDIQLTKKQ LLSMNPRKLT NKAQMDTRKE VIATRKEALV NAIKLPPALN QFTNYLSEED LKEVSDILSK FRPETRKERK QRLQNERKG PKPIIVKQGI KHVTKLIEEK KAKFVLIACD VDPIEVVLYL PTLCVKMGIP YALVKTKEDL GKIIKKKPTT C LCLCDVSS ...String:
MSDIQLTKKQ LLSMNPRKLT NKAQMDTRKE VIATRKEALV NAIKLPPALN QFTNYLSEED LKEVSDILSK FRPETRKERK QRLQNERKG PKPIIVKQGI KHVTKLIEEK KAKFVLIACD VDPIEVVLYL PTLCVKMGIP YALVKTKEDL GKIIKKKPTT C LCLCDVSS DIKSKFENVI AKCNGQFKDN YESAMKVWGS PKEKVKEE

UniProtKB: 60S ribosomal protein L8

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Macromolecule #15: Ribosomal protein L34

MacromoleculeName: Ribosomal protein L34 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 12.020348 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MVERQVVRRG STFNTRSNKR KKVRTPGNRL VFQDVKKKGK GPKCGQCGIK LAGVKEARPA AFSRMRKSCR KVNRILGGVQ CAKCVQEKV LDAFLNAEEK RMKELEV

UniProtKB: Ribosomal protein L34

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Macromolecule #16: 60S ribosomal protein L9

MacromoleculeName: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 21.53823 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MVYVYVEEKV QIPEGCKVEV VNKKVTVTGP KGTVSKDFSH IFVFIDIHEE NVRCRLWNCR KQERSKLITC SKLIKNMIDG SMSGYFYEL KAAYKHFPIT FEIKENGKLL IVKNFLGQKW AREFKMIGDT IIKQGVTKDH LILEGISKED VSQTAGLIQN N CLPKNLDR RIFQDGVYIA TKGLIEEVK

UniProtKB: 60S ribosomal protein L9

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Macromolecule #17: Ribosomal L29 protein

MacromoleculeName: Ribosomal L29 protein / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 14.183866 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MKLLAKDLRN NSIEELEKNL VDAKSEMLQI RQKKQSAILK PNELRNGRRN VAVILTIIRE KKYQQLLEKY KDADNIPKEL KPRMNRRKR QELTKKQKKI SKVGKTKLYT KGNKLIYAFE

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Macromolecule #18: S60 ribosomal protein L10

MacromoleculeName: S60 ribosomal protein L10 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 25.142328 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MGRRPGRCYR YCKNKPYIKS RFCRGVPDPK IRIHDLGNKN APCVDLPLNV NLISYSKEQL SSESLESARI AVNKYMMVNA GKDNYHVRI NVHPYHILRI NKMLSCAGAD RLQTGMRLAY GKPYGTAARV RFGQRIISIR TKDSNLKMAM EALRRARYKF P GKYEVQKS ...String:
MGRRPGRCYR YCKNKPYIKS RFCRGVPDPK IRIHDLGNKN APCVDLPLNV NLISYSKEQL SSESLESARI AVNKYMMVNA GKDNYHVRI NVHPYHILRI NKMLSCAGAD RLQTGMRLAY GKPYGTAARV RFGQRIISIR TKDSNLKMAM EALRRARYKF P GKYEVQKS NEYGFSGIER SQFEELKNNG KLINMGDHVK IIKEKGTVER YVKLCENAIQ

UniProtKB: S60 ribosomal protein L10

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Macromolecule #19: 60S ribosomal protein L36

MacromoleculeName: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 11.350697 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MKINKLNNAS KALSYPVKKL AKTEKAPLTE KQQRSKELKK FAMSIAREVG GLAPYELRAT ELLRRDEDKR CKKFMKKRLG SLKMAKKKI ELLAEEMRA

UniProtKB: 60S ribosomal protein L36

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Macromolecule #20: 60S ribosomal protein L11

MacromoleculeName: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 19.484965 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MAEENPMNKI KIQKLCLNIC TGGGEILNKA SKVLEQLTGQ KPRLSKSRLT IRSFGIRRNE KIACHVTVRG EKAMEILEKG IKIKEYELI DSNFSTQGSF GFGIKEHIDM GIKYEPDVGI FGMNFYVVLE KPGIRVSKRK RVKSRVGNKQ RVTKDEAKNW F VKKYEGIV LGK

UniProtKB: 60S ribosomal protein L11

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Macromolecule #21: eL37 LJJ

MacromoleculeName: eL37 LJJ / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus)
Molecular weightTheoretical: 10.517657 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MSKGTPSNGK KNKRNHTTCC RCGSVSFHKQ KGKCSSCAYP EKKMKKMQNR KAMLKKTTGT GKMRHMKKER ISRRAGYPGN PILKEIRNK IKS

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Macromolecule #22: 60S ribosomal protein L13

MacromoleculeName: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 19.513141 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MRGNQMLPNN HFNKGSMKFK NWFNQPMRAL KRKEVREQKS KICYPNPLKK LRPVVRCCSM RYNKKVRLGR GFTKMEIEKA GMDIKYAIS VGIAVDLRRK NKNEEGLEEN VKRLMDYKNK LKFFTTVREA RDNKIEQYKG EIMKPVNIIP EVKGIKVEEI A TIYGN

UniProtKB: 60S ribosomal protein L13

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Macromolecule #23: 60S ribosomal protein L39

MacromoleculeName: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 6.227581 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MGSIKTRRIK VQLVSALKKN RCIPQWKRAC PGFKQQYNFT KRSWKRKTLK AC

UniProtKB: 60S ribosomal protein L39

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Macromolecule #24: eL14 LM0

MacromoleculeName: eL14 LM0 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 14.158396 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
KYFSYPLMYF IKPGCLIKKK FSIYTSIVIS VIDNNSVVIQ SYDKSDNGDV ISIDREVINV SKIVPIGNID IKNKSKKEID GILVEENRN LKNKDDVLLM NDFERFKEQL KKEVEDMVIE EMA

UniProtKB: Uncharacterized protein

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Macromolecule #25: Ubiquitin

MacromoleculeName: Ubiquitin / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 14.563868 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MQLFLKSPNG TSTIDLALEN TLENVLTNEG VSTENNRFIY NGKQMDTNRC LFEYGFKGLE TIYVTGRICG GKLTDDDKVL AITKFEKQV CRKCYARLPI RATHCRKRAC GYSSKLRGKK KRRDESKK

UniProtKB: Ubiquitin

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Macromolecule #26: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 24.200426 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MGYLQYLKEI RRDKQSSVMR YLLRVRCWEY RQNTAIHRAS GPTYLDKARM LGYRNIPGMC IFRARVRRGA RPKKVLKGNT HGKPSKSGV YQRKPAKRLQ AFAEIRVGRK CSNMRVFNSY WVGQDRVYKY FEVIMVDPYC DRIKNDKKIN WLCKPVMKHR E ARGLTNGT ...String:
MGYLQYLKEI RRDKQSSVMR YLLRVRCWEY RQNTAIHRAS GPTYLDKARM LGYRNIPGMC IFRARVRRGA RPKKVLKGNT HGKPSKSGV YQRKPAKRLQ AFAEIRVGRK CSNMRVFNSY WVGQDRVYKY FEVIMVDPYC DRIKNDKKIN WLCKPVMKHR E ARGLTNGT KTSRGLGKGI RYNQTKGGSK RAAWRNRNTQ SLRRYR

UniProtKB: Ribosomal protein L15

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Macromolecule #27: Ribosomal protein L13A

MacromoleculeName: Ribosomal protein L13A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 22.885139 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MIGKRIVIDA IGHVAGKLAG HIAKELMCGI EVVIVHAENV MFTGPIKRGV GKFNSHLNKS CSYNPLRGPF HLRSPSMHLM KVIKRKLPH RKSKGQIALK RLQVFDGCPT EYLSVEKMVC PRDKLEYTTS PIRKSYKLGD LMIKFGWKYE SVVEKMNQNE E QFKIEETE ...String:
MIGKRIVIDA IGHVAGKLAG HIAKELMCGI EVVIVHAENV MFTGPIKRGV GKFNSHLNKS CSYNPLRGPF HLRSPSMHLM KVIKRKLPH RKSKGQIALK RLQVFDGCPT EYLSVEKMVC PRDKLEYTTS PIRKSYKLGD LMIKFGWKYE SVVEKMNQNE E QFKIEETE KQKVKEQKIE ELKKSNKFQQ RVEEIMVQMA

UniProtKB: Ribosomal protein L13A

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Macromolecule #28: 60S ribosomal protein L44

MacromoleculeName: 60S ribosomal protein L44 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 11.99051 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MVNIPKTRRT YCIKCAYHTE FKVGLLKKGK DSPRKQGARR YARKQRGVHG QTKPILKRKA KTTKKVILKI ECTKCKSSQL KVRGRAKHV EFGAEKKVKG EALIY

UniProtKB: 60S ribosomal protein L44

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Macromolecule #29: 60S ribosomal protein L17

MacromoleculeName: 60S ribosomal protein L17 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 19.11449 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MAHKYSCDIS EATSVRSQYN NIKIKFKNTR EVCNIIKTME LNKAIAYLND VLRFKKCIPV FRFAKKCGRT PQAKEFGTDK GKWPVKSVA FVKKMLEDLL VEAEKKGLGT SELVIKHIQV NQAPKIYGRK HGAFGRIKPY NKSPSHIEVI AVKREHNIKE G DVVEVKE

UniProtKB: 60S ribosomal protein L17

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Macromolecule #30: 60S ribosomal protein L37a

MacromoleculeName: 60S ribosomal protein L37a / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 10.151127 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MSRKTKKVGI TGKYGSRYGS SLKRRAMICM NAQSKRYCCS FCGKTTVKRE VIGIWSCRFC GKKVSGGAYV PITNQQVEYK QVINRMKME

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Macromolecule #31: 60S ribosomal protein L18

MacromoleculeName: 60S ribosomal protein L18 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 21.378133 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MLKQKMFEHY KKKAILFDES RVPRTKNVFY QSLFSLYKKV CENTENEIVH KITKRLTMSR TERHPLKLSN IIEQANGKEI VVFVGKVLD DDKILEIPKI TIVALKISKQ AKEKLTLNGG EFYTLDKLFY ISKDLKDVAL VKGDKNNRKC TRYFGASGDK G SETYPRTR KGARRGERRI KNKKK

UniProtKB: 60S ribosomal protein L18

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Macromolecule #32: 60S ribosomal protein L19

MacromoleculeName: 60S ribosomal protein L19 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 19.549994 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MKLPRVKELS ADILKCGVRR IYLDTFEPSK LSAATSRDDI RKLIKDGLIV RKAQKIHSRY HANKLMREKE KGRHSGPGKV KGSKNARMP EKDKWIKRIR DLRNTLKELR DKGEITKTEH KMYYQKTKGN GYKNSAALMG AIKQKHDDER RMKEIEEQAK A LKIAASEK

UniProtKB: 60S ribosomal protein L19

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Macromolecule #33: 60S ribosomal protein L20

MacromoleculeName: 60S ribosomal protein L20 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 20.043385 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MAINEYKVYG IKIDDNGVVG DEVYAHIVFA TNELLAKSSF NKILKKQKKI KPKNTKVIKV EEIKEDNKDL DVKNYGIDYY YKSKRCDHK GYKEFRGVSR CHAVDKLLCD IGGRHSVKRN LIFIMKVKEL TVDEIKKDKI KIFAMEDVEY PAYRNVLRSN K LFVSENEK LFN

UniProtKB: 60S ribosomal protein L20

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Macromolecule #34: 60s ribosomal protein L21

MacromoleculeName: 60s ribosomal protein L21 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 18.34442 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MVSSHGYRRQ TRNLFSQAFR KHGMPNTSTY LTQYAVGEYV DIKVNPAIHK GMPHKYYHGR TGIIKEVNRG SVIVLFNRIL ANKTVERKV IVGIEHIRKS KCQDEYLKRL EINKKIIEEA EKKGEVPVLF KRQPKGPKPA VEMLMNNIIE LSYDKVESQ

UniProtKB: 60s ribosomal protein L21

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Macromolecule #35: 60S ribosomal protein L22

MacromoleculeName: 60S ribosomal protein L22 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 12.854773 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MSEAAVQDQS ITYTLDCTKC TEQSLFSTDD LKEYLLGNIK YNGKKGQLGK KVLLSNTGHA VDVFFAKKTS KRYLKYLIKK FLRAKGLSD WVRPIAGDKQ SYKFSFFSKQ AGEE

UniProtKB: Large ribosomal subunit protein eL22

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Macromolecule #36: Ribosomal protein L23

MacromoleculeName: Ribosomal protein L23 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 15.239987 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MPGKGKQPLD AIKNRYGFTR GIQVGTVLAC ADNSGAKMLK TIGVKGVSGR LNRLPAAGPG DVIVASVVKG RSDMRKKIVL AVIIRQKQM IRRKDGVRLQ FEESAAVIIS NKGDLKGTQI SGPVLREVCD IWPKISSQAG SIY

UniProtKB: Ribosomal protein L23

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Macromolecule #37: Ribosomal protein L24E

MacromoleculeName: Ribosomal protein L24E / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 15.342161 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MFYEGTCVYS GYPVPRGKGT YFVSLDDKSS LFFSSKYKNF SDKKIKAKRV KQTLMSKQFC GKVESVHTEK KKKIVVQKIV RGFPALPKD AVEKIREQNK DYKKKIVDEK KVQHGKKVGK IEQKQMKQKQ KY

UniProtKB: Ribosomal protein L24E

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Macromolecule #38: 60S ribosomal protein L23a

MacromoleculeName: 60S ribosomal protein L23a / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 12.557947 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MPKLNRHKAS RLDKKIKQKK LIEKPLGFSS LDIIKYGIGH EGAAKLIEEA GTLTFVVDQR ADKINVKKAF EEIYGEKVKK VNINNTMKG VKKAYIRLVE LGNAAVVATK AGIL

UniProtKB: 60S ribosomal protein L23a

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Macromolecule #39: 60S ribosomal protein L26

MacromoleculeName: 60S ribosomal protein L26 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 15.026874 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MKFNKNVTAS RRKNRKAHFT SIGLERTKLM NAPLSKDLKE EYGIKRIAVR PGDMVKVVSG EFKKKEGVVM KVNRHDIKIE VEGCFVQKE DGKKINKGIH PSNLRIIKLS MEKDRAEIIN KKVEAMKSEV KA

UniProtKB: 60S ribosomal protein L26

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Macromolecule #40: 60S ribosomal protein L27

MacromoleculeName: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 18.247842 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MKPTFIIRIN LFFPFKKYFF LFHIFFLFFI FFCPMSETLF EKNMFVLLNR GRFAGNKGII VDTDLENNRI IIAGVSKIPK NKKRKTIVF LKKMNPMHIM ATTYKRDIGL SLDNLDSIFE DKVKKAVALN RVREIFNKNK NNEGFAWLYK KLEI

UniProtKB: 60S ribosomal protein L27

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Macromolecule #42: 40S ribosomal protein S0

MacromoleculeName: 40S ribosomal protein S0 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 26.201186 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MAVRVVPLSD KVAKAMVQCC CHLGGVTSST SLKEYIFGKR EEDKVNIIDI KKMWEKLNLA ARMICSIPNP KDIVVVSSKD FGRKAVIKF AESIGATAVT GRFIPGSFSN YEIKGVREPR LVLVSDCFAD KQVVKEASYV NAPCIAFCNT DNDIEFVDMV I PMNNRSQS ...String:
MAVRVVPLSD KVAKAMVQCC CHLGGVTSST SLKEYIFGKR EEDKVNIIDI KKMWEKLNLA ARMICSIPNP KDIVVVSSKD FGRKAVIKF AESIGATAVT GRFIPGSFSN YEIKGVREPR LVLVSDCFAD KQVVKEASYV NAPCIAFCNT DNDIEFVDMV I PMNNRSQS AIGAGFCILS KLVNYMKGNI DKVEDKLRDH IELYTYRDSD ELEQLYQEQK IEEEADMVLK DESDE

UniProtKB: 40S ribosomal protein S0

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Macromolecule #43: 40S ribosomal protein S26

MacromoleculeName: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 11.974061 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MTKKRSNNGR SKKNRGHTRC VRCEHCSAMV PNDKAIKKFV VRSVIDAASL DDLKIATIYE EYEVPKSYYK LNYCVSCAVH IKLVRVRSR WERKIRKEQR VSA

UniProtKB: 40S ribosomal protein S26

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Macromolecule #44: eS1 SB0

MacromoleculeName: eS1 SB0 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus)
Molecular weightTheoretical: 25.934658 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MAIKADNPKM RKGMKKGGKK KVEKDSFLKK EKYNLRIENF PVREVGATLV NKQSTVAALE KKLVGRVFEA NQGDITTSDS ANCYRKFSF KIDSLAGTNA HAIFNGMCLT SEKIKGMVKR GLTLIEGVKD VVTKEGITLR ILTIAQSKKE KNSTKKTAYA K TSVVKEVR ...String:
MAIKADNPKM RKGMKKGGKK KVEKDSFLKK EKYNLRIENF PVREVGATLV NKQSTVAALE KKLVGRVFEA NQGDITTSDS ANCYRKFSF KIDSLAGTNA HAIFNGMCLT SEKIKGMVKR GLTLIEGVKD VVTKEGITLR ILTIAQSKKE KNSTKKTAYA K TSVVKEVR KNIFRIVDNE LNGKDIEQIV KKLQMETVGK EIEKECQKII PLQNCHVLKV KVVKRPVKEE VE

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Macromolecule #45: eS27 SBB

MacromoleculeName: eS27 SBB / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus)
Molecular weightTheoretical: 9.189923 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MIAKNLLYPT VEEIKKNNKR KQLFQEVKGY FMEVKCAGCA TLNVCYSHSQ KTISCKGCSM VILRSTGGKA KISNECSYMV IS

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Macromolecule #46: 40S ribosomal protein S2

MacromoleculeName: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 27.241541 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MNSEETTKTS SQEQRRPKRA PREEAEWFPK TSLGKLVKAG RITLDEIFGH SLCIREPEII DYFLKATLKE EVLVIKSVQK QTKAGQKTR MKVVLALGDH NNYIGIGSGV ARDVVSAINA AKVVAKCSMR PIKKGFWGNE IGEAHTVPVK ALGKCGSVKV Q VTPAPKGT ...String:
MNSEETTKTS SQEQRRPKRA PREEAEWFPK TSLGKLVKAG RITLDEIFGH SLCIREPEII DYFLKATLKE EVLVIKSVQK QTKAGQKTR MKVVLALGDH NNYIGIGSGV ARDVVSAINA AKVVAKCSMR PIKKGFWGNE IGEAHTVPVK ALGKCGSVKV Q VTPAPKGT GLVAGGVSKT IFKLAGIKDI FTSSKGQTCT TENFARASLY AITNSTNFNM PNVIEEKVVE LNPILKNAEF LI EQEQRDL R

UniProtKB: 40S ribosomal protein S2

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Macromolecule #47: eS28 SCC

MacromoleculeName: eS28 SCC / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus)
Molecular weightTheoretical: 7.288479 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MSEEVYIGEV LQTLEKTGPG GGLTMCQVQL KDQKRTLYRA IMGPIAVGDL VALLDCEREH RRGRF

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Macromolecule #48: 40S ribosomal protein S3

MacromoleculeName: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 27.516459 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MQNKSIFPQL IFFNIFFQII FFTLMFNPEA SSIKNRFVER GCYYAEINEF LSKLLQKEGF SGLSLRLHER PIRVVIRLEK TTEVLKDNS VLLKQIKALI GKRLQEDVSN IDILIEMVKN KGLCPAIQAE FIRSKFEENI PYRRSVVSAI KNIKSAGGQG C VIEVSGKL ...String:
MQNKSIFPQL IFFNIFFQII FFTLMFNPEA SSIKNRFVER GCYYAEINEF LSKLLQKEGF SGLSLRLHER PIRVVIRLEK TTEVLKDNS VLLKQIKALI GKRLQEDVSN IDILIEMVKN KGLCPAIQAE FIRSKFEENI PYRRSVVSAI KNIKSAGGQG C VIEVSGKL KGQRARSVKT IDGKIIHAGQ PKEEFMRYAT TSVLLKQGII GIKVGIMLPQ DDEGIKGPVY DMVDKIKVLP IK EE

UniProtKB: 40S ribosomal protein S3

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Macromolecule #49: 40S ribosomal protein S29

MacromoleculeName: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 7.891175 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MNKQMIKYNA DLETTFTVER KDFGRTNRRC RACFAYNGVI RKYDLMICRR CFREYAGDIG FKIYD

UniProtKB: 40S ribosomal protein S29

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Macromolecule #50: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 31.442154 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MVRGPRKHLK RLNAPKSWSL DKNGGVFAPR TSTGPHSMQE SIPLCLLLTR ILKVASNNKE IKHIMKNRLI TVNGNIRTDS RYPVGIMDV LSIKKTNEHY RLLYNIAGKF VLHKITEEEA QYRIAKVTNK KVVKGNIPHT YTSCGGSFKY ADPSISIGDS V KIDIKTSK ...String:
MVRGPRKHLK RLNAPKSWSL DKNGGVFAPR TSTGPHSMQE SIPLCLLLTR ILKVASNNKE IKHIMKNRLI TVNGNIRTDS RYPVGIMDV LSIKKTNEHY RLLYNIAGKF VLHKITEEEA QYRIAKVTNK KVVKGNIPHT YTSCGGSFKY ADPSISIGDS V KIDIKTSK IVENSSLEVG KVVYLIKGKN IGCLGVITGI EKREGTHDIV NIVDKVGRNF STIFSNILVV GADDNPWVTF TK DEGIKYS EYEQSIKEYG PMNEEKEEES IPEVVETSEV ETR

UniProtKB: 40S ribosomal protein S4

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Macromolecule #51: eS30 SEE

MacromoleculeName: eS30 SEE / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus)
Molecular weightTheoretical: 6.81601 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MAKAISLNKA GKVRGQTPKV EKSEKEKALT GRAAWRAKFK RRQELFTGVG KFKFNKQGDF

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Macromolecule #52: 40S ribosomal protein S5

MacromoleculeName: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 21.982785 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MRYTETSGDI KLFGRYPYTD LEVKDITLEK YINVASKVIL PHTSSRLNTK KFGRTKIPVV ERFVNAMMRK GRNNGKKTLA MRIVKDAFF IIEGVTGQNP IQILVDAICN SGPREDSARI GKGGVMKRSS VDVSPLRRIN IAIFLLAKGV RNSTFKDIKM L PEIIAEEL ...String:
MRYTETSGDI KLFGRYPYTD LEVKDITLEK YINVASKVIL PHTSSRLNTK KFGRTKIPVV ERFVNAMMRK GRNNGKKTLA MRIVKDAFF IIEGVTGQNP IQILVDAICN SGPREDSARI GKGGVMKRSS VDVSPLRRIN IAIFLLAKGV RNSTFKDIKM L PEIIAEEL INAAKNTQSS FAVKKKEEIE RIAKSNR

UniProtKB: 40S ribosomal protein S5

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Macromolecule #53: Ubiquitin/40s ribosomal protein S27a fusion

MacromoleculeName: Ubiquitin/40s ribosomal protein S27a fusion / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 16.937791 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MQIFVKTLTG KTITLEVEST DSIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGKKKK KKKSYTTPK KVKKPKQDIK QIILTQFSVN KDGECKPLRN SCSTCGPSVF LGRSGNKEYC GRCYTVAILN K

UniProtKB: Ubiquitin/40s ribosomal protein S27a fusion

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Macromolecule #54: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 26.238879 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MKLNISYPTN GTHKTFKLDF KAEQRLYGKK TLDTFDGSII KPEWEGLQFQ ITGGSDVQGF PMIKTLLTDK RKKLLLKKGD KGFKPVNRK GLRKRKTVRG SIIASDISMV CVSLIDAKEV AIEGLTDVVV GATHWPKRFN KLKAKAGFSE DADVTAEQVL K VIKDAILE ...String:
MKLNISYPTN GTHKTFKLDF KAEQRLYGKK TLDTFDGSII KPEWEGLQFQ ITGGSDVQGF PMIKTLLTDK RKKLLLKKGD KGFKPVNRK GLRKRKTVRG SIIASDISMV CVSLIDAKEV AIEGLTDVVV GATHWPKRFN KLKAKAGFSE DADVTAEQVL K VIKDAILE VNGGDIKKLP KLKVSRYQTE KQKERKAEKI KLIEERKKKS EKERADFLKK YPDWQKKFAT KA

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Macromolecule #55: Guanine nucleotide binding protein beta subunit

MacromoleculeName: Guanine nucleotide binding protein beta subunit / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 36.182824 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString: MISLNEKGSQ YVHKQMVTCL EVPNEGNSDI LYTASRDKKI YSILLKPEED KVGKYIKSYE GHSGFVNCIT TNSSNDKIIS GSSDNTARI FDVESQKSVV LKGHTRDITS ISINTEDNKI ITGSVDGKIM LWNTQGKCAE VFDGECQNTH TSWINCVAFR P FNSNIVVS ...String:
MISLNEKGSQ YVHKQMVTCL EVPNEGNSDI LYTASRDKKI YSILLKPEED KVGKYIKSYE GHSGFVNCIT TNSSNDKIIS GSSDNTARI FDVESQKSVV LKGHTRDITS ISINTEDNKI ITGSVDGKIM LWNTQGKCAE VFDGECQNTH TSWINCVAFR P FNSNIVVS GSEDGTLKIW DIEEKRVMET FINGFSVTEI KEKGIEIERK SAVTSLSMSP DGSLCAYGSK NSMVYVFKFD DN KCVINFD AGSTVKALAF GLTDVSIACA TESEIILWDI LKNVRIARID YSSYGNGVYC TSLVWAKDML LAGFSDGRIT AYE RTIIEE

UniProtKB: Guanine nucleotide binding protein beta subunit

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Macromolecule #56: 40S ribosomal protein S7

MacromoleculeName: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 19.132023 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MSQSQSEMQV AEVIKKVCGS VIKDSLQSAL KTLRIKLISD SDGSNIMVIT LPLPLLDAIQ QYFSEIQNKL QKQFSEFQIF FIREAVYNP NNTLNKKLQE NWLKDLCFPS VVNQRMTVVK EGGNIKEEKV FVERKSEFTE EEFYSMKLVF KELTGRDVEY G LRYY

UniProtKB: 40S ribosomal protein S7

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Macromolecule #57: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 19.912068 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MGISRSNRHK RKETGGKKGL HEKKRKNNCG RPPSNTRIGE RRVKQVRVRG GNIKHRALRL DTGNIACKTH NFTLNTSIQN VMYHPSNNE FMRTNILTKS AVVKINGQPF KHELEKDQQF ESKDPVLFDN LRKGNCYAII TSRPGQVGMA DGYLLQGEEL K FYMEKFKK KSVKV

UniProtKB: 40S ribosomal protein S8

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Macromolecule #58: 40S ribosomal protein S9

MacromoleculeName: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 21.365959 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MKFFSTLMVK SKGIKKIVVP HRPFERERLV SEMYIVGKYG LRNKRELWTI QKMCDSFKLR AKDLLITPDE KEFTLSSRRL LKKLAEWGV FMEDVDYNNK QSLTNNLEKL LDLAPTSFLE RRLQHRIFDL GLASSVHQAR AFIHARRVKI NGSVVNKPGY L VKDGEDGF IEVVESKRSA LVAAEE

UniProtKB: 40S ribosomal protein S9

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Macromolecule #59: 40S ribosomal protein S10

MacromoleculeName: 40S ribosomal protein S10 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 12.702538 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MQLSREDQVA IRKYLFRESI CLVKDKDNGN HPELNINNKQ VMKFLRSYVS RGLVEKVFVW QHAYFLLKSE GVDVLREELY LDETEVPLT YMRETYEAPK AEVGKVQE

UniProtKB: 40S ribosomal protein S10

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Macromolecule #60: 40S ribosomal protein S11

MacromoleculeName: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 18.109406 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MLNEISVSKV FPKQAGVFNN PYEKDSKRRV KDVSEIYKAP ESARNSTYID KNCPFTGEIK IKNNFMKGEV LKMKQPTTIV VVKRYLHYV KKYKRYERRY KNVSVHLSPC FKGLVNVGDT VICGETRPLS KTKRFVVVGF EKKEIKEKKL KMLSEI

UniProtKB: 40S ribosomal protein S11

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Macromolecule #61: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 14.343721 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MSEIVVPMGS EELTLDQALY QVCRISNANN RMVKGIRQVS KKLLDSNLKL VLLSNEIDER SANIIISLAK KTNIPLIKVD DSITLGRYA GFEKRTISDE IKVAKCGCAG IVDYVQPSQG QVFLMNAIKQ G

UniProtKB: 40S ribosomal protein S12

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Macromolecule #62: 40S ribosomal protein S13

MacromoleculeName: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 16.19199 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MARMYSNGKG QSKSVTPYNL TYPTYIKHSK EEIVDVILNL ARKGTPPSQI GNILRDNYGV GKSFAVTKTK ILTLLKIAGL KPVIPEDLN NIVKKCVNIK AHLAKYTNDK SSKYQLIQMQ SCLYRVARYY KSKNELPAGW KPPF

UniProtKB: 40S ribosomal protein S13

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Macromolecule #63: 40S ribosomal protein S14

MacromoleculeName: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 14.708 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MSEPMVEQRL NFALLFIKAT KNDTIIHATD LTGAETLAKS SGGSKVKAHR DERDPYAAML ATQDVAAVLK EKGYNAVHIK IRGKGGNQS KIIGPGGSTV IRTMMRSEFK LGRVEDVTPV ATDSTRKKGG RRGRRL

UniProtKB: 40S ribosomal protein S14

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Macromolecule #64: Ribosomal protein S19

MacromoleculeName: Ribosomal protein S19 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 18.514629 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MAKKQNFQKK KKSVAVESMG DASARSKRTF KEFSYKGVNL ETLTSMSISE FKELLPSKLR RSINRGFSAA ERDFIKKCIK ARSENTGLE RPEQINTNTR NLPILPTMIG LQIGVYSGRD FMPMVIKEEM LGRRLRDYVE TRKLCTHGRP GVGATSSSKF V PLK

UniProtKB: Ribosomal protein S19

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Macromolecule #65: 40S ribosomal protein S16

MacromoleculeName: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 16.415074 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MSIAQVITKG TRKTAISKCV CSESNEFQLM VDNMTLDVHH DNFLVAKIKE IMKIIGYEHL ENLKFELTTR GGGPISRAYA IRQAFAKAV ISYFGKYYDE QRKQDIKEQI MKFDRAALVA DPRRVEPKKY GGPAARAKYT KSYR

UniProtKB: 40S ribosomal protein S16

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Macromolecule #66: eS17 SR0

MacromoleculeName: eS17 SR0 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus)
Molecular weightTheoretical: 14.055251 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MGQIKGKSVR TASKVIVERY FNRLTNDFYD NRNVVIDVAE IRSKKLRNKV SGCVTKLYKR VKKNGVPGLY IKEHEEEREK KESFIPKIS ILDEDKVEVD EVTMEMINQY EINGDYVLAK K

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Macromolecule #67: 40S ribosomal protein S18

MacromoleculeName: 40S ribosomal protein S18 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 18.171129 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MQAKESKLVF NNNPLPIQAM IRMFNTNIDG KKQIIKALTT IRGIGDRLSL AILRRAKIPT DKRAGELTED ELNEINEVIL NPQKYDIPE WMLNHQNDPI DGTTKHLIGN QIEGTIRLWN ERGKKIGLVR AFRTSLGLKV RGQRTKSNGR GGRTMGVSRK K

UniProtKB: 40S ribosomal protein S18

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Macromolecule #68: 40S Ribosomal protein S19

MacromoleculeName: 40S Ribosomal protein S19 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 16.603318 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MNTEIFKVRH DTFIPALSEY LRTNKTVVPI KNFDIIKTSR GKETSPLDIN WYYTRAASII RKLLISKAKN EKPLSIVRFS SEYGCAKDR GNRPNKHVRA SKGIIIKILN DLQEKGWVEK KENGWTLKKE AEEVSKQLIE KLIN

UniProtKB: 40S Ribosomal protein S19

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Macromolecule #69: 40S ribosomal protein S20

MacromoleculeName: 40S ribosomal protein S20 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 13.777075 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MNDVEKKYDI ETPVPEKLIN VELRLLCSNK KLLSDLSNRY YNYLFQNSPT AKKPRVLQPF ICDVTTRKSP CGQGTATWSK YKMIIHGRM FALQMTHQML KDSAAFLQNT DVEVRLTLKG

UniProtKB: 40S ribosomal protein S20

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Macromolecule #70: Ribosomal protein S21E

MacromoleculeName: Ribosomal protein S21E / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 7.768887 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MNSFQRVCDF SLKPISSQDR SSVQINMVQL DERGRATKDL MIFNVSGTVR KNGKIDSMLY ERINEME

UniProtKB: Ribosomal protein S21E

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Macromolecule #71: 40S ribosomal protein S15A

MacromoleculeName: 40S ribosomal protein S15A / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 14.620056 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MMDKLANLCI TINNANRAQK RSVVIRRISK VCRLFLESMQ KHGYITDIVY IDDHREGKAV VGLSGRLNRC GAICPRYDVE LKDVERYRA NLLPSRGFGH LIVTSSKGLI DHRECIQHNT GGKILGYFY

UniProtKB: 40S ribosomal protein S15A

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Macromolecule #72: uS12 SX0

MacromoleculeName: uS12 SX0 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus)
Molecular weightTheoretical: 15.822722 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MYGLLAARNL KKNRHKRRYA DKDFAKKARG DIYQNAVGYS APHAKGIVTE KLGVEAKQPN SAIRKCVRVH IVRLNKKVIA YVPHDGGLN QIEINDEVIV EGLGKKGCRS KGDIPGVRFR VCKVQKVSLK ALVTKKKERP AR

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Macromolecule #73: 40s ribosomal protein s24

MacromoleculeName: 40s ribosomal protein s24 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 17.189895 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MVSSFSNFQS FSIEILSRYD NELLHRSEVD LNISHENAMT PSRSDVAQKL AQLFNTGVNN IVIRGTKTFF GSGNSKVKVK VYNNMDQLK KIEHFCELKR IAEKSKTEMQ LVEKLPRRTR KDNRKKRNKM WGTEKRRMKK AEKKNSR

UniProtKB: 40s ribosomal protein s24

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Macromolecule #74: 40S ribosomal protein S25

MacromoleculeName: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spraguea lophii 42_110 (fungus) / Strain: 42_110
Molecular weightTheoretical: 14.777071 KDa
Recombinant expressionOrganism: Spraguea lophii (fungus)
SequenceString:
MAKKVLESKT SKAEKISNST KSKKKWSSGK VAEAVKRHER VEDDIFNKIV KDVKNMRILT RTTLSERYNL SLNISIKLLR FLEENQIIK LISSHRGSKI YAGSKYEPLK VVEQPKEVEA TVDNDQEWA

UniProtKB: 40S ribosomal protein S25

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Macromolecule #75: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 75 / Number of copies: 199 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #76: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 76 / Number of copies: 160 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #77: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 77 / Number of copies: 9 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration10 mg/mL
BufferpH: 7.5
Component:
ConcentrationFormulaName
25.0 mMHEPESHEPES
100.0 mMCH3CO2KPotassium Acetate
1.0 mMDTTDithiothreitol
15.0 mMMg(C2H3O2)2Magnessium Acetate

Details: Solution with HEPES, Potassium Acetate and Magnesium acetate was autoclaved. DTT was added fresh just before sample preparation.
GridModel: Quantifoil / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 1 sec. / Pretreatment - Atmosphere: OTHER / Pretreatment - Pressure: 0.026000000000000002 kPa / Details: 20 mA. Carbon coated grid
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 288.15 K / Instrument: FEI VITROBOT MARK IV / Details: blot force -1 and blot time 4 s.
DetailsMonomeric ribosomes from Spraguea lophii

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Electron microscopy #1

MicroscopeTFS KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.2 µm
Microscopy ID1
Image recordingImage recording ID: 1 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 41.5 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Electron microscopy #1~

MicroscopeTFS TALOS
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.2 µm
Microscopy ID1
Image recordingImage recording ID: 2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 41.34 e/Å2

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Image processing

Particle selectionNumber selected: 977879
Startup modelType of model: NONE
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final 3D classificationSoftware - Name: RELION (ver. 3.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.79 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 285940
Image recording ID2
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

6rm3


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: RECIPROCAL / Protocol: AB INITIO MODEL
Output model

PDB-7qca:
Spraguea lophii ribosome

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