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Open data
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Basic information
Entry | Database: PDB / ID: 7piw | |||||||||
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Title | Stacked stretched Dunaliella PSII | |||||||||
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![]() | PHOTOSYNTHESIS / green algae / photosystem II / thylakoid / oxygen evolving complex / cryo-EM / stacking | |||||||||
Function / homology | ![]() oxygen evolving activity / photosystem II / photosystem II reaction center / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / response to herbicide / photosystem II / chlorophyll binding / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity ...oxygen evolving activity / photosystem II / photosystem II reaction center / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / response to herbicide / photosystem II / chlorophyll binding / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / phosphate ion binding / chloroplast thylakoid membrane / photosynthetic electron transport in photosystem II / photosynthesis / membrane => GO:0016020 / electron transfer activity / protein stabilization / iron ion binding / heme binding / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4 Å | |||||||||
![]() | Caspy, I. / Fadeeva, M. / Mazor, Y. / Nelson, N. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of photosystem II reveals conformational flexibility of stacked and unstacked supercomplexes. Authors: Ido Caspy / Maria Fadeeva / Yuval Mazor / Nathan Nelson / ![]() ![]() Abstract: Photosystem II (PSII) generates an oxidant whose redox potential is high enough to enable water oxidation , a substrate so abundant that it assures a practically unlimited electron source for life on ...Photosystem II (PSII) generates an oxidant whose redox potential is high enough to enable water oxidation , a substrate so abundant that it assures a practically unlimited electron source for life on earth . Our knowledge on the mechanism of water photooxidation was greatly advanced by high-resolution structures of prokaryotic PSII . Here, we show high-resolution cryogenic electron microscopy (cryo-EM) structures of eukaryotic PSII from the green alga at two distinct conformations. The conformers are also present in stacked PSII, exhibiting flexibility that may be relevant to the grana formation in chloroplasts of the green lineage. CP29, one of PSII associated light-harvesting antennae, plays a major role in distinguishing the two conformations of the supercomplex. We also show that the stacked PSII dimer, a form suggested to support the organisation of thylakoid membranes , can appear in many different orientations providing a flexible stacking mechanism for the arrangement of grana stacks in thylakoids. Our findings provide a structural basis for the heterogenous nature of the eukaryotic PSII on multiple levels. | |||||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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PDBx/mmCIF format | ![]() | 3.1 MB | Display | ![]() |
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PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 13455MC ![]() 7pi0C ![]() 7pi5C ![]() 7pinC ![]() 7pnkC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Assembly
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Components
-Photosystem II ... , 13 types, 47 molecules AaA1a1BbB1b1VvV1v1CcC1c1DdD1d1HhH1h1IiI1i1Jj...
#1: Protein | Mass: 37291.488 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #2: Protein | Mass: 53289.516 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #3: Protein/peptide | Mass: 3217.949 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #4: Protein | Mass: 49128.992 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #5: Protein | Mass: 38965.383 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #8: Protein | Mass: 7124.381 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #9: Protein/peptide | Mass: 3929.648 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #10: Protein/peptide | Mass: 3737.500 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #11: Protein/peptide | Mass: 4159.991 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #12: Protein/peptide | Mass: 4417.203 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() #13: Protein/peptide | Mass: 3437.093 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #16: Protein/peptide | Mass: 3478.194 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #26: Protein/peptide | Mass: 3307.979 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Cytochrome b559 subunit ... , 2 types, 8 molecules EeE1e1FfF1f1
#6: Protein | Mass: 8743.847 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #7: Protein/peptide | Mass: 3549.299 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Protein , 9 types, 32 molecules OoO1o1PpP1p1ZzZ1z1NnN1n1GgG1g1RrSsS1s1YyY1y1R1r1
#14: Protein | Mass: 25874.908 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #15: Protein | Mass: 20425.707 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #19: Protein | Mass: 6430.616 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #20: Protein | Mass: 24075.082 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #21: Protein | Mass: 23719.543 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #22: Protein | Mass: 21748.461 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #23: Protein | Mass: 26233.607 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #24: Protein | Mass: 23537.566 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #27: Protein | Mass: 21828.438 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Protein/peptide , 3 types, 12 molecules WwW1w1XxX1x1UuU1u1
#17: Protein/peptide | Mass: 4698.315 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #18: Protein/peptide | Mass: 2854.407 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #25: Protein/peptide | Mass: 3219.625 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Sugars , 2 types, 18 molecules ![](data/chem/img/DGD.gif)
![](data/chem/img/LMT.gif)
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#40: Sugar | ChemComp-DGD / #55: Sugar | |
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+Non-polymers , 28 types, 685 molecules ![](data/chem/img/OEX.gif)
![](data/chem/img/FE2.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/CLA.gif)
![](data/chem/img/PHO.gif)
![](data/chem/img/BCR.gif)
![](data/chem/img/SQD.gif)
![](data/chem/img/LMG.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/C7Z.gif)
![](data/chem/img/DGA.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/LHG.gif)
![](data/chem/img/LMK.gif)
![](data/chem/img/BCT.gif)
![](data/chem/img/PL9.gif)
![](data/chem/img/HEM.gif)
![](data/chem/img/RRX.gif)
![](data/chem/img/CHL.gif)
![](data/chem/img/LUT.gif)
![](data/chem/img/XAT.gif)
![](data/chem/img/NEX.gif)
![](data/chem/img/LPX.gif)
![](data/chem/img/3PH.gif)
![](data/chem/img/SPH.gif)
![](data/chem/img/4RF.gif)
![](data/chem/img/ERG.gif)
![](data/chem/img/PTY.gif)
![](data/chem/img/FE2.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/CLA.gif)
![](data/chem/img/PHO.gif)
![](data/chem/img/BCR.gif)
![](data/chem/img/SQD.gif)
![](data/chem/img/LMG.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/C7Z.gif)
![](data/chem/img/DGA.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/LHG.gif)
![](data/chem/img/LMK.gif)
![](data/chem/img/BCT.gif)
![](data/chem/img/PL9.gif)
![](data/chem/img/HEM.gif)
![](data/chem/img/RRX.gif)
![](data/chem/img/CHL.gif)
![](data/chem/img/LUT.gif)
![](data/chem/img/XAT.gif)
![](data/chem/img/NEX.gif)
![](data/chem/img/LPX.gif)
![](data/chem/img/3PH.gif)
![](data/chem/img/SPH.gif)
![](data/chem/img/4RF.gif)
![](data/chem/img/ERG.gif)
![](data/chem/img/PTY.gif)