+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 7mjo | ||||||
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タイトル | Vascular KATP channel: Kir6.1 SUR2B quatrefoil-like conformation 1 | ||||||
要素 |
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キーワード | TRANSPORT PROTEIN / KATP / potassium channel / vascular | ||||||
機能・相同性 | 機能・相同性情報 cardiac pacemaker cell differentiation / atrioventricular node cell differentiation / substrate-dependent cell migration, cell contraction / reactive gliosis / ATP sensitive Potassium channels / ABC-family proteins mediated transport / response to resveratrol / ATP-activated inward rectifier potassium channel activity / membrane repolarization during ventricular cardiac muscle cell action potential / glutamate secretion, neurotransmission ...cardiac pacemaker cell differentiation / atrioventricular node cell differentiation / substrate-dependent cell migration, cell contraction / reactive gliosis / ATP sensitive Potassium channels / ABC-family proteins mediated transport / response to resveratrol / ATP-activated inward rectifier potassium channel activity / membrane repolarization during ventricular cardiac muscle cell action potential / glutamate secretion, neurotransmission / inward rectifying potassium channel / NLRP3 inflammasome complex assembly / sulfonylurea receptor activity / ventricular cardiac muscle tissue development / circulatory system development / voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization / CAMKK-AMPK signaling cascade / cardiac conduction / voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / coronary vasculature development / ATPase-coupled monoatomic cation transmembrane transporter activity / vasculature development / nervous system process / cardiac muscle cell contraction / response to stress / synaptic assembly at neuromuscular junction / inorganic cation transmembrane transport / regulation of monoatomic ion transmembrane transport / syntaxin binding / sulfonylurea receptor binding / neuromuscular process / Ion homeostasis / response to ATP / establishment of cell polarity / myofibril / heterocyclic compound binding / response to exogenous dsRNA / blood vessel development / fat cell differentiation / transmission of nerve impulse / p38MAPK cascade / potassium ion import across plasma membrane / action potential / monoatomic cation transmembrane transport / ATPase-coupled transmembrane transporter activity / protein secretion / potassium channel regulator activity / ABC-type transporter activity / heart morphogenesis / fatty acid transport / presynaptic active zone membrane / potassium ion transmembrane transport / T-tubule / negative regulation of blood pressure / response to endoplasmic reticulum stress / sarcomere / regulation of heart rate / acrosomal vesicle / response to cytokine / kidney development / response to ischemia / determination of adult lifespan / calcium ion transmembrane transport / microglial cell activation / response to insulin / potassium ion transport / sarcolemma / transmembrane transport / regulation of blood pressure / vasodilation / presynapse / heart development / gene expression / fibroblast proliferation / defense response to virus / transmembrane transporter binding / adaptive immune response / response to lipopolysaccharide / cell population proliferation / response to hypoxia / inflammatory response / response to xenobiotic stimulus / glutamatergic synapse / apoptotic process / ATP hydrolysis activity / protein-containing complex / ATP binding / identical protein binding / membrane / plasma membrane 類似検索 - 分子機能 | ||||||
生物種 | Rattus norvegicus (ドブネズミ) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4 Å | ||||||
データ登録者 | Sung, M.W. / Shyng, S.L. | ||||||
資金援助 | 米国, 1件
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引用 | ジャーナル: Proc Natl Acad Sci U S A / 年: 2021 タイトル: Vascular K channel structural dynamics reveal regulatory mechanism by Mg-nucleotides. 著者: Min Woo Sung / Zhongying Yang / Camden M Driggers / Bruce L Patton / Barmak Mostofian / John D Russo / Daniel M Zuckerman / Show-Ling Shyng / 要旨: Vascular tone is dependent on smooth muscle K channels comprising pore-forming Kir6.1 and regulatory SUR2B subunits, in which mutations cause Cantú syndrome. Unique among K isoforms, they lack ...Vascular tone is dependent on smooth muscle K channels comprising pore-forming Kir6.1 and regulatory SUR2B subunits, in which mutations cause Cantú syndrome. Unique among K isoforms, they lack spontaneous activity and require Mg-nucleotides for activation. Structural mechanisms underlying these properties are unknown. Here, we determined cryogenic electron microscopy structures of vascular K channels bound to inhibitory ATP and glibenclamide, which differ informatively from similarly determined pancreatic K channel isoform (Kir6.2/SUR1). Unlike SUR1, SUR2B subunits adopt distinct rotational "propeller" and "quatrefoil" geometries surrounding their Kir6.1 core. The glutamate/aspartate-rich linker connecting the two halves of the SUR-ABC core is observed in a quatrefoil-like conformation. Molecular dynamics simulations reveal MgADP-dependent dynamic tripartite interactions between this linker, SUR2B, and Kir6.1. The structures captured implicate a progression of intermediate states between MgADP-free inactivated, and MgADP-bound activated conformations wherein the glutamate/aspartate-rich linker participates as mobile autoinhibitory domain, suggesting a conformational pathway toward K channel activation. | ||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 7mjo.cif.gz | 511.2 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb7mjo.ent.gz | 383.4 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 7mjo.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 7mjo_validation.pdf.gz | 1.6 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 7mjo_full_validation.pdf.gz | 1.7 MB | 表示 | |
XML形式データ | 7mjo_validation.xml.gz | 84.2 KB | 表示 | |
CIF形式データ | 7mjo_validation.cif.gz | 123.9 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/mj/7mjo ftp://data.pdbj.org/pub/pdb/validation_reports/mj/7mjo | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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-要素
-タンパク質 , 2種, 6分子 ABCDEG
#1: タンパク質 | 分子量: 48023.871 Da / 分子数: 4 / 由来タイプ: 組換発現 / 由来: (組換発現) Rattus norvegicus (ドブネズミ) / 遺伝子: Kcnj8 / 発現宿主: Chlorocebus aethiops (ミドリザル) / 参照: UniProt: Q63664 #2: タンパク質 | 分子量: 174488.562 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) Rattus norvegicus (ドブネズミ) / 遺伝子: Abcc9, Sur2 / 発現宿主: Chlorocebus aethiops (ミドリザル) / 参照: UniProt: Q63563 |
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-糖 , 1種, 2分子
#3: 多糖 |
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-非ポリマー , 5種, 21分子
#4: 化合物 | #5: 化合物 | ChemComp-ATP / #6: 化合物 | ChemComp-PTY / #7: 化合物 | #8: 化合物 | ChemComp-GBM / | |
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-詳細
研究の焦点であるリガンドがあるか | Y |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 | 名称: Vascular KATP channel: Kir6.1 SUR2B quatrefoil-like conformation 2 タイプ: COMPLEX / Entity ID: #1-#2 / 由来: RECOMBINANT |
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分子量 | 値: 0.889 MDa / 実験値: YES |
由来(天然) | 生物種: Rattus norvegicus (ドブネズミ) |
由来(組換発現) | 生物種: Chlorocebus aethiops (ミドリザル) |
緩衝液 | pH: 7.5 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
試料支持 | グリッドのタイプ: Quantifoil R2/1 |
急速凍結 | 凍結剤: ETHANE |
-電子顕微鏡撮影
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: SPOT SCAN |
電子レンズ | モード: BRIGHT FIELD |
撮影 | 電子線照射量: 40 e/Å2 / フィルム・検出器のモデル: GATAN K3 (6k x 4k) |
-解析
EMソフトウェア |
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CTF補正 | タイプ: NONE | ||||||||||||||||||||
3次元再構成 | 解像度: 4 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 71880 / 対称性のタイプ: POINT | ||||||||||||||||||||
原子モデル構築 | PDB-ID: 6BAA |