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Yorodumi- PDB-7d0a: Acinetobacter MlaFEDB complex in ADP-vanadate trapped Vclose conf... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7d0a | ||||||
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Title | Acinetobacter MlaFEDB complex in ADP-vanadate trapped Vclose conformation | ||||||
Components |
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Keywords | MEMBRANE PROTEIN | ||||||
Function / homology | Function and homology information phospholipid transporter activity / ATP-binding cassette (ABC) transporter complex / phospholipid binding / ATP hydrolysis activity / ATP binding / membrane Similarity search - Function | ||||||
Biological species | Acinetobacter baumannii (bacteria) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4 Å | ||||||
Authors | Zhang, Y.Y. / Fan, Q.X. / Chi, X.M. / Zhou, Q. / Li, Y.Y. | ||||||
Funding support | China, 1items
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Citation | Journal: Cell Discov / Year: 2020 Title: Cryo-EM structures of Acinetobacter baumannii glycerophospholipid transporter. Authors: Yuanyuan Zhang / Qiongxuan Fan / Ximin Chi / Qiang Zhou / Yanyan Li / | ||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 7d0a.cif.gz | 405.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7d0a.ent.gz | 325.2 KB | Display | PDB format |
PDBx/mmJSON format | 7d0a.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7d0a_validation.pdf.gz | 1.2 MB | Display | wwPDB validaton report |
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Full document | 7d0a_full_validation.pdf.gz | 1.3 MB | Display | |
Data in XML | 7d0a_validation.xml.gz | 77.8 KB | Display | |
Data in CIF | 7d0a_validation.cif.gz | 113.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/d0/7d0a ftp://data.pdbj.org/pub/pdb/validation_reports/d0/7d0a | HTTPS FTP |
-Related structure data
Related structure data | 30528MC 7d06C 7d08C 7d09C M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
-Protein , 4 types, 12 molecules ADBECFGHIJKL
#1: Protein | Mass: 27322.443 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acinetobacter baumannii (bacteria) / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: V5V9F4 #2: Protein | Mass: 30070.455 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acinetobacter baumannii (bacteria) / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: A0A086HZU3 #3: Protein | Mass: 11913.760 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acinetobacter baumannii (bacteria) / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: V5V9K5 #4: Protein | Mass: 24135.322 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acinetobacter baumannii (bacteria) / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: V5V921 |
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-Non-polymers , 2 types, 4 molecules
#5: Chemical | #6: Chemical | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Acinetobacter MlaFEDB complex in ADP-vanadate trapped Vclose conformation Type: COMPLEX / Entity ID: #1-#4 / Source: RECOMBINANT |
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Source (natural) | Organism: Acinetobacter baumannii (bacteria) |
Source (recombinant) | Organism: Escherichia coli K-12 (bacteria) |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Alignment procedure: COMA FREE |
Specimen holder | Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-Processing
EM software | Name: RELION / Version: 3.0.6 / Category: 3D reconstruction |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
3D reconstruction | Resolution: 4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 33995 / Symmetry type: POINT |