- PDB-6ofg: In vitro polymerized PrgI V67A filaments -
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基本情報
登録情報
データベース: PDB / ID: 6ofg
タイトル
In vitro polymerized PrgI V67A filaments
要素
Protein PrgI
キーワード
PROTEIN TRANSPORT / helical reconstruction / Salmonella / Type III secretion system
機能・相同性
機能・相同性情報
type III protein secretion system complex / protein secretion by the type III secretion system / cell surface / extracellular region / identical protein binding 類似検索 - 分子機能
Type III secretion, needle-protein-like / Type III secretion, needle-protein-like superfamily / Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF / Type III secretion system, needle protein 類似検索 - ドメイン・相同性
Type III secretion system apparatus / SPI-1 type 3 secretion system needle filament protein 類似検索 - 構成要素
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI030492
米国
引用
ジャーナル: PLoS Biol / 年: 2019 タイトル: A polymorphic helix of a Salmonella needle protein relays signals defining distinct steps in type III secretion. 著者: Emily Z Guo / Daniel C Desrosiers / Jan Zalesak / James Tolchard / Mélanie Berbon / Birgit Habenstein / Thomas Marlovits / Antoine Loquet / Jorge E Galán / 要旨: Type III protein-secretion machines are essential for the interactions of many pathogenic or symbiotic bacterial species with their respective eukaryotic hosts. The core component of these machines ...Type III protein-secretion machines are essential for the interactions of many pathogenic or symbiotic bacterial species with their respective eukaryotic hosts. The core component of these machines is the injectisome, a multiprotein complex that mediates the selection of substrates, their passage through the bacterial envelope, and ultimately their delivery into eukaryotic target cells. The injectisome is composed of a large cytoplasmic complex or sorting platform, a multiring base embedded in the bacterial envelope, and a needle-like filament that protrudes several nanometers from the bacterial surface and is capped at its distal end by the tip complex. A characteristic feature of these machines is that their activity is stimulated by contact with target host cells. The sensing of target cells, thought to be mediated by the distal tip of the needle filament, generates an activating signal that must be transduced to the secretion machine by the needle filament. Here, through a multidisciplinary approach, including solid-state NMR (SSNMR) and cryo electron microscopy (cryo-EM) analyses, we have identified critical residues of the needle filament protein of a Salmonella Typhimurium type III secretion system that are involved in the regulation of the activity of the secretion machine. We found that mutations in the needle filament protein result in various specific phenotypes associated with different steps in the type III secretion process. More specifically, these studies reveal an important role for a polymorphic helix of the needle filament protein and the residues that line the lumen of its central channel in the control of type III secretion.
P: Protein PrgI Q: Protein PrgI R: Protein PrgI K: Protein PrgI L: Protein PrgI M: Protein PrgI N: Protein PrgI O: Protein PrgI F: Protein PrgI G: Protein PrgI H: Protein PrgI I: Protein PrgI J: Protein PrgI A: Protein PrgI B: Protein PrgI C: Protein PrgI D: Protein PrgI E: Protein PrgI