Ministry of Education, Youth and Sports of the Czech Republic
LM2015043
Czech Republic
Citation
Journal: Sci Adv / Year: 2019 Title: Structural basis for the multitasking nature of the potato virus Y coat protein. Authors: Andreja Kežar / Luka Kavčič / Martin Polák / Jiří Nováček / Ion Gutiérrez-Aguirre / Magda Tušek Žnidarič / Anna Coll / Katja Stare / Kristina Gruden / Maja Ravnikar / David ...Authors: Andreja Kežar / Luka Kavčič / Martin Polák / Jiří Nováček / Ion Gutiérrez-Aguirre / Magda Tušek Žnidarič / Anna Coll / Katja Stare / Kristina Gruden / Maja Ravnikar / David Pahovnik / Ema Žagar / Franci Merzel / Gregor Anderluh / Marjetka Podobnik / Abstract: Potato virus Y (PVY) is among the most economically important plant pathogens. Using cryoelectron microscopy, we determined the near-atomic structure of PVY's flexuous virions, revealing a previously ...Potato virus Y (PVY) is among the most economically important plant pathogens. Using cryoelectron microscopy, we determined the near-atomic structure of PVY's flexuous virions, revealing a previously unknown lumenal interplay between extended carboxyl-terminal regions of the coat protein units and viral RNA. RNA-coat protein interactions are crucial for the helical configuration and stability of the virion, as revealed by the unique near-atomic structure of RNA-free virus-like particles. The structures offer the first evidence for plasticity of the coat protein's amino- and carboxyl-terminal regions. Together with mutational analysis and in planta experiments, we show their crucial role in PVY infectivity and explain the ability of the coat protein to perform multiple biological tasks. Moreover, the high modularity of PVY virus-like particles suggests their potential as a new molecular scaffold for nanobiotechnological applications.
Mass: 29935.797 Da / Num. of mol.: 35 / Source method: isolated from a natural source / Source: (natural) Potato virus Y / References: UniProt: A0A0A7DJ81
#2: RNA chain
... RNA (5'-R(P*UP*UP*UP*UP*U)-3')
Mass: 1485.872 Da / Num. of mol.: 35 / Source method: isolated from a natural source Details: Due to helical averaging during cryo-EM data processing, all nucleotides were assigned the uracil base. Source: (natural) Potato virus Y
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Experimental details
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Experiment
Experiment
Method: ELECTRON MICROSCOPY
EM experiment
Aggregation state: HELICAL ARRAY / 3D reconstruction method: helical reconstruction
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