+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 6gsr | ||||||
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タイトル | Single Particle Cryo-EM map of human Transferrin receptor 1 - H-Ferritin complex at 5.5 Angstrom resolution. | ||||||
要素 |
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キーワード | METAL BINDING PROTEIN / Transferrin Receptor 1 / Ferritin / Complex / Single Particle Cryo-EM | ||||||
機能・相同性 | 機能・相同性情報 transferrin receptor activity / negative regulation of mitochondrial fusion / transferrin transport / Transferrin endocytosis and recycling / Differentiation of keratinocytes in interfollicular epidermis in mammalian skin / positive regulation of isotype switching / iron ion sequestering activity / : / response to copper ion / response to iron ion ...transferrin receptor activity / negative regulation of mitochondrial fusion / transferrin transport / Transferrin endocytosis and recycling / Differentiation of keratinocytes in interfollicular epidermis in mammalian skin / positive regulation of isotype switching / iron ion sequestering activity / : / response to copper ion / response to iron ion / RND1 GTPase cycle / response to manganese ion / RND2 GTPase cycle / autolysosome / Scavenging by Class A Receptors / RHOB GTPase cycle / Golgi Associated Vesicle Biogenesis / ferroxidase / RHOJ GTPase cycle / RHOC GTPase cycle / RHOQ GTPase cycle / CDC42 GTPase cycle / RHOH GTPase cycle / transport across blood-brain barrier / RHOG GTPase cycle / RHOA GTPase cycle / intracellular sequestering of iron ion / ferroxidase activity / RAC2 GTPase cycle / RAC3 GTPase cycle / positive regulation of bone resorption / response to retinoic acid / clathrin-coated pit / positive regulation of T cell proliferation / negative regulation of fibroblast proliferation / positive regulation of B cell proliferation / Hsp70 protein binding / RAC1 GTPase cycle / ferric iron binding / response to nutrient / osteoclast differentiation / cellular response to leukemia inhibitory factor / acute-phase response / Iron uptake and transport / clathrin-coated endocytic vesicle membrane / positive regulation of protein-containing complex assembly / ferrous iron binding / HFE-transferrin receptor complex / recycling endosome / receptor internalization / positive regulation of protein localization to nucleus / recycling endosome membrane / double-stranded RNA binding / extracellular vesicle / melanosome / cellular response to xenobiotic stimulus / Cargo recognition for clathrin-mediated endocytosis / tertiary granule lumen / positive regulation of peptidyl-serine phosphorylation / Clathrin-mediated endocytosis / virus receptor activity / positive regulation of NF-kappaB transcription factor activity / iron ion transport / cytoplasmic vesicle / basolateral plasma membrane / positive regulation of canonical NF-kappaB signal transduction / intracellular iron ion homeostasis / ficolin-1-rich granule lumen / early endosome / blood microparticle / response to hypoxia / endosome membrane / intracellular signal transduction / endosome / iron ion binding / positive regulation of protein phosphorylation / immune response / negative regulation of cell population proliferation / external side of plasma membrane / intracellular membrane-bounded organelle / Neutrophil degranulation / positive regulation of gene expression / protein-containing complex binding / negative regulation of apoptotic process / protein kinase binding / perinuclear region of cytoplasm / cell surface / protein homodimerization activity / RNA binding / extracellular space / extracellular exosome / extracellular region / identical protein binding / membrane / nucleus / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 5.5 Å | ||||||
データ登録者 | Testi, C. / Montemiglio, L.C. / Vallone, B. / Des Georges, A. / Boffi, A. / Mancia, F. / Baiocco, P. | ||||||
引用 | ジャーナル: Nat Commun / 年: 2019 タイトル: Cryo-EM structure of the human ferritin-transferrin receptor 1 complex. 著者: Linda Celeste Montemiglio / Claudia Testi / Pierpaolo Ceci / Elisabetta Falvo / Martina Pitea / Carmelinda Savino / Alessandro Arcovito / Giovanna Peruzzi / Paola Baiocco / Filippo Mancia / ...著者: Linda Celeste Montemiglio / Claudia Testi / Pierpaolo Ceci / Elisabetta Falvo / Martina Pitea / Carmelinda Savino / Alessandro Arcovito / Giovanna Peruzzi / Paola Baiocco / Filippo Mancia / Alberto Boffi / Amédée des Georges / Beatrice Vallone / 要旨: Human transferrin receptor 1 (CD71) guarantees iron supply by endocytosis upon binding of iron-loaded transferrin and ferritin. Arenaviruses and the malaria parasite exploit CD71 for cell invasion ...Human transferrin receptor 1 (CD71) guarantees iron supply by endocytosis upon binding of iron-loaded transferrin and ferritin. Arenaviruses and the malaria parasite exploit CD71 for cell invasion and epitopes on CD71 for interaction with transferrin and pathogenic hosts were identified. Here, we provide the molecular basis of the CD71 ectodomain-human ferritin interaction by determining the 3.9 Å resolution single-particle cryo-electron microscopy structure of their complex and by validating our structural findings in a cellular context. The contact surfaces between the heavy-chain ferritin and CD71 largely overlap with arenaviruses and Plasmodium vivax binding regions in the apical part of the receptor ectodomain. Our data account for transferrin-independent binding of ferritin to CD71 and suggest that select pathogens may have adapted to enter cells by mimicking the ferritin access gate. | ||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 6gsr.cif.gz | 1.3 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb6gsr.ent.gz | 表示 | PDB形式 | |
PDBx/mmJSON形式 | 6gsr.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 6gsr_validation.pdf.gz | 933.5 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 6gsr_full_validation.pdf.gz | 933 KB | 表示 | |
XML形式データ | 6gsr_validation.xml.gz | 104.4 KB | 表示 | |
CIF形式データ | 6gsr_validation.cif.gz | 153.6 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/gs/6gsr ftp://data.pdbj.org/pub/pdb/validation_reports/gs/6gsr | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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-要素
#1: タンパク質 | 分子量: 21124.459 Da / 分子数: 24 / 由来タイプ: 組換発現 詳細: Chain Am, E14, R22, L29, R79, F81, K119 no side chains 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: FTH1, FTH, FTHL6, OK/SW-cl.84, PIG15 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P02794, ferroxidase #2: タンパク質 | 分子量: 71807.258 Da / 分子数: 2 / 由来タイプ: 組換発現 / 詳細: Chain Ab, L209 and Y211 no side chains. / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: TFRC / 発現宿主: Homo sapiens (ヒト) / 参照: UniProt: P02786 |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 |
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由来(天然) | 生物種: Homo sapiens (ヒト) | ||||||||||||||||||||||||
由来(組換発現) | 生物種: Escherichia coli (大腸菌) | ||||||||||||||||||||||||
緩衝液 | pH: 7.2 | ||||||||||||||||||||||||
試料 | 濃度: 0.2 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES | ||||||||||||||||||||||||
急速凍結 | 凍結剤: ETHANE / 湿度: 100 % / 凍結前の試料温度: 277 K |
-電子顕微鏡撮影
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: OTHER |
電子レンズ | モード: OTHER |
撮影 | 電子線照射量: 40 e/Å2 フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) |
-解析
CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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粒子像の選択 | 選択した粒子像数: 25870 |
対称性 | 点対称性: C1 (非対称) |
3次元再構成 | 解像度: 5.5 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 25870 / 対称性のタイプ: POINT |
原子モデル構築 | プロトコル: RIGID BODY FIT |