- PDB-5w68: Type II secretin from Enteropathogenic Escherichia coli - GspD -
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基本情報
登録情報
データベース: PDB / ID: 5w68
タイトル
Type II secretin from Enteropathogenic Escherichia coli - GspD
要素
Putative type II secretion protein
キーワード
MEMBRANE PROTEIN / Type 2 secretin / outer membrane complex / homo oligomer
機能・相同性
機能・相同性情報
protein secretion by the type II secretion system / type II protein secretion system complex / cell outer membrane / membrane => GO:0016020 類似検索 - 分子機能
Type II secretion system protein GspD / GspD/PilQ family / NolW-like / Bacterial type II/III secretion system short domain / NolW-like superfamily / Type II/III secretion system / Bacterial type II and III secretion system protein 類似検索 - ドメイン・相同性
National Health and Medical Research Council (NHMRC, Australia)
1092262
オーストラリア
Australian Research Council (ARC)
FL130100038
オーストラリア
引用
ジャーナル: J Bacteriol / 年: 2018 タイトル: Structure and Membrane Topography of the Vibrio-Type Secretin Complex from the Type 2 Secretion System of Enteropathogenic Escherichia coli. 著者: Iain D Hay / Matthew J Belousoff / Rhys A Dunstan / Rebecca S Bamert / Trevor Lithgow / 要旨: The β-barrel assembly machinery (BAM) complex is the core machinery for the assembly of β-barrel membrane proteins, and inhibition of BAM complex activity is lethal to bacteria. Discovery of ...The β-barrel assembly machinery (BAM) complex is the core machinery for the assembly of β-barrel membrane proteins, and inhibition of BAM complex activity is lethal to bacteria. Discovery of integral membrane proteins that are key to pathogenesis and yet do not require assistance from the BAM complex raises the question of how these proteins assemble into bacterial outer membranes. Here, we address this question through a structural analysis of the type 2 secretion system (T2SS) secretin from enteropathogenic O127:H6 strain E2348/69. Long β-strands assemble into a barrel extending 17 Å through and beyond the outer membrane, adding insight to how these extensive β-strands are assembled into the outer membrane. The substrate docking chamber of this secretin is shown to be sufficient to accommodate the substrate mucinase SteC. In order to cause disease, bacterial pathogens inhibit immune responses and induce pathology that will favor their replication and dissemination. In Gram-negative bacteria, these key attributes of pathogenesis depend on structures assembled into or onto the outer membrane. One of these is the T2SS. The -type T2SS mediates cholera toxin secretion in , and in O127:H6 strain E2348/69, the same machinery mediates secretion of the mucinases that enable the pathogen to penetrate intestinal mucus and thereby establish deadly infections.
A: Putative type II secretion protein B: Putative type II secretion protein C: Putative type II secretion protein D: Putative type II secretion protein E: Putative type II secretion protein F: Putative type II secretion protein G: Putative type II secretion protein H: Putative type II secretion protein I: Putative type II secretion protein J: Putative type II secretion protein K: Putative type II secretion protein L: Putative type II secretion protein M: Putative type II secretion protein N: Putative type II secretion protein O: Putative type II secretion protein