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- PDB-11hf: Bacteriophage Goslar Tail Assembly -

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Basic information

Entry
Database: PDB / ID: 11hf
TitleBacteriophage Goslar Tail Assembly
Components
  • Tail Tube of Bacteriophage Goslar
  • Tail sheath protein gp29 gp29PR domain-containing protein
KeywordsVIRAL PROTEIN / Tail Assembly
Function / homology: / Phage tail tube protein / : / Bacteriophage phiKZ, gp29PR / Uncharacterized protein / Tail sheath protein gp29 gp29PR domain-containing protein
Function and homology information
Biological speciesGoslarvirus
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.39 Å
AuthorsBasu, D. / Gu, Y. / Corbett, K.D.
Funding support United States, 1items
OrganizationGrant numberCountry
Howard Hughes Medical Institute (HHMI)Emerging Pathogens Initiative United States
CitationJournal: To Be Published
Title: Bacteriophage Goslar Tail Assembly
Authors: Basu, D. / Gu, Y. / Corbett, K.D.
History
DepositionFeb 24, 2026Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 19, 2026Provider: repository / Type: Initial release
Revision 1.0Aug 19, 2026Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
AG: Tail Tube of Bacteriophage Goslar
AH: Tail Tube of Bacteriophage Goslar
AI: Tail Tube of Bacteriophage Goslar
AJ: Tail Tube of Bacteriophage Goslar
AK: Tail Tube of Bacteriophage Goslar
AL: Tail Tube of Bacteriophage Goslar
AM: Tail Tube of Bacteriophage Goslar
AN: Tail Tube of Bacteriophage Goslar
AO: Tail Tube of Bacteriophage Goslar
AP: Tail Tube of Bacteriophage Goslar
AQ: Tail Tube of Bacteriophage Goslar
AR: Tail Tube of Bacteriophage Goslar
L: Tail sheath protein gp29 gp29PR domain-containing protein
M: Tail sheath protein gp29 gp29PR domain-containing protein
N: Tail sheath protein gp29 gp29PR domain-containing protein
O: Tail sheath protein gp29 gp29PR domain-containing protein
P: Tail sheath protein gp29 gp29PR domain-containing protein
Q: Tail sheath protein gp29 gp29PR domain-containing protein
R: Tail sheath protein gp29 gp29PR domain-containing protein
S: Tail sheath protein gp29 gp29PR domain-containing protein
T: Tail sheath protein gp29 gp29PR domain-containing protein
U: Tail sheath protein gp29 gp29PR domain-containing protein
V: Tail sheath protein gp29 gp29PR domain-containing protein
W: Tail sheath protein gp29 gp29PR domain-containing protein


Theoretical massNumber of molelcules
Total (without water)1,317,21624
Polymers1,317,21624
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails (eV)
d_1ens_1chain "AP"
d_2ens_1chain "AH"
d_3ens_1chain "AI"
d_4ens_1chain "AJ"
d_5ens_1chain "AK"
d_6ens_1chain "AL"
d_7ens_1chain "AM"
d_8ens_1chain "AN"
d_9ens_1chain "AO"
d_10ens_1chain "AQ"
d_11ens_1chain "AG"
d_12ens_1chain "AR"
d_1ens_2chain "M"
d_2ens_2chain "N"
d_3ens_2chain "L"
d_4ens_2chain "O"
d_5ens_2chain "P"
d_6ens_2chain "Q"
d_7ens_2chain "R"
d_8ens_2chain "S"
d_9ens_2chain "T"
d_10ens_2chain "U"
d_11ens_2chain "V"
d_12ens_2chain "W"

NCS domain segments:

Component-ID: 1

Dom-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_1ens_1METMETVALVALAPJ1 - 2961 - 296
d_2ens_1METMETVALVALAHB1 - 2961 - 296
d_3ens_1METMETVALVALAIC1 - 2961 - 296
d_4ens_1METMETVALVALAJD1 - 2961 - 296
d_5ens_1METMETVALVALAKE1 - 2961 - 296
d_6ens_1METMETVALVALALF1 - 2961 - 296
d_7ens_1METMETVALVALAMG1 - 2961 - 296
d_8ens_1METMETVALVALANH1 - 2961 - 296
d_9ens_1METMETVALVALAOI1 - 2961 - 296
d_10ens_1METMETVALVALAQK1 - 2961 - 296
d_11ens_1METMETVALVALAGA1 - 2961 - 296
d_12ens_1METMETVALVALARL1 - 2961 - 296
d_1ens_2LEULEUARGARGMN3 - 6823 - 682
d_2ens_2LEULEUARGARGNO3 - 6823 - 682
d_3ens_2LEULEUARGARGLM3 - 6823 - 682
d_4ens_2LEULEUARGARGOP3 - 6823 - 682
d_5ens_2LEULEUARGARGPQ3 - 6823 - 682
d_6ens_2LEULEUARGARGQR3 - 6823 - 682
d_7ens_2LEULEUARGARGRS3 - 6823 - 682
d_8ens_2LEULEUARGARGST3 - 6823 - 682
d_9ens_2LEULEUARGARGTU3 - 6823 - 682
d_10ens_2LEULEUARGARGUV3 - 6823 - 682
d_11ens_2LEULEUARGARGVW3 - 6823 - 682
d_12ens_2LEULEUARGARGWX3 - 6823 - 682

NCS ensembles :
ID
ens_1
ens_2

NCS oper:
IDCodeMatrixVector
1given(-0.147350979592, 0.989063569869, 0.00639871635476), (-0.989069185097, -0.147381376085, 0.00456914382295), (0.00546222532232, -0.00565550535246, 0.999969089199)44.7290245861, 626.916128966, 38.9326662649
2given(0.78283717812, 0.622226496664, 0.000373367681265), (-0.622217007641, 0.782821624509, 0.00602491523138), (0.00345658160233, -0.00494884335953, 0.999981780331)-119.337951377, 245.045690325, 39.3509606147
3given(0.932861692806, -0.360100049003, -0.00984971086011), (0.360155552873, 0.932881200231, 0.00454356576199), (0.00755247183557, -0.00778594650856, 0.999941167872)128.608488297, -87.2822365825, 38.902585206
4given(0.153177183239, -0.9881980149, -0.00119744872984), (0.988198637408, 0.153177608058, -0.000270952299372), (0.000451176856582, -0.00114181349318, 0.99999924635)539.662792814, -41.3389665605, 39.3233721704
5given(-0.781170570376, -0.624317459547, 0.000499683036997), (0.624312232297, -0.781166866683, -0.00354443241813), (0.00260318687517, -0.00245684806146, 0.999993593637)706.949211744, 341.191903511, 38.9912906196
6given(-0.999990797875, 0.00407372618894, -0.00134496085105), (-0.00407060171599, -0.999989029134, -0.00231771714846), (-0.00135438784071, -0.00231222102059, 0.999996409627)587.152853825, 590.141901464, 1.01680140002
7given(-0.496402892928, 0.868091889207, -0.000799865896497), (-0.868091408596, -0.4964034854, -0.000941278159323), (-0.00121417215447, 0.00022710351144, 0.999999237105)184.813170778, 695.62364538, 0.253716014389
8given(0.503173606671, 0.864182979943, 0.00202453136738), (-0.864185350128, 0.503172098755, 0.00123274443465), (4.66290619217E-5, -0.00236985481185, 0.999997190803)-108.825930067, 399.828566626, 0.60858165531
9given(0.501601049329, -0.865093432627, -0.00312091926217), (0.865098820984, 0.501594951728, 0.00255623398736), (-0.000645943888068, -0.00398211322448, 0.999991862732)401.829499422, -108.481738385, 1.2920247589
10given(-0.930988095215, 0.365046594761, 0.00146636326291), (-0.365048944292, -0.930986321414, -0.00193329008111), (0.000659423179201, -0.00233516441118, 0.99999705608)459.895978, 675.676305734, 39.6064717662
11given(-0.498374177906, -0.866961772124, 0.00068152172107), (0.866961439881, -0.498374603879, -0.000784836082854), (0.00102007599899, 0.000199711015026, 0.99999945978)695.120137658, 185.963458453, -0.403356180563
12given(0.499398746739, -0.866354732868, -0.00551077052799), (0.866352859338, 0.499420028219, -0.00351547019703), (0.00579783341605, -0.00301865039348, 0.999978636211)403.485671993, -106.399694305, -1.07153518418
13given(0.498722771942, 0.866687675781, -0.0113167749881), (-0.866699554193, 0.498800755042, 0.00544881009315), (0.0103672324641, 0.00709079816365, 0.999921117425)-104.049191177, 400.727030641, -6.25444400794
14given(-0.494946123862, -0.868915199184, 0.00383550536886), (0.868916269581, -0.494955300649, -0.00194082914689), (0.00358481965773, 0.00237212715373, 0.999990760998)693.768929452, 184.244049525, -2.16230393482
15given(-0.99990825925, -0.00416746472739, 0.0128881853068), (0.0041823375249, -0.999990618636, 0.00112724971692), (0.0128833666246, 0.00118104904322, 0.999916308492)585.545557291, 586.080722569, -4.91600263123
16given(-0.502653625822, 0.864485989384, 0.00181840767812), (-0.864478990899, -0.502656883151, 0.00348311854605), (0.00392514231821, 0.000178826931714, 0.99999228061)187.268120738, 695.019906875, -1.49648991922
17given(0.783167979034, 0.621810169144, -0.000173679642334), (-0.621797098307, 0.78315326797, 0.00627115656818), (0.00403548670586, -0.00480337551807, 0.999980321022)-118.910420199, 244.622680826, 39.5230195986
18given(0.930433886395, -0.366458141995, -0.00110145940873), (0.366459789196, 0.930429030334, 0.00300706052789), (-7.71320043014E-5, -0.00320151159632, 0.999994872174)128.630718825, -88.1694603923, 40.1835713471
19given(0.149461712832, -0.988729036834, -0.00872285041813), (0.988765149746, 0.14947483069, -0.000868123255946), (0.00216218526009, -0.00849509931111, 0.999961578383)543.16179452, -40.2594510328, 41.418220743
20given(-0.780567554969, -0.625069843806, -0.00140801054496), (0.625071362158, -0.780566536963, -0.0012936705495), (-0.000290411466778, -0.00188990432703, 0.99999817196)707.835975681, 339.858046111, 39.8563607502
21given(-0.925248725795, 0.379285089975, 0.00759051629559), (-0.379264591489, -0.925279466263, 0.00403471877057), (0.00855365753856, 0.000854304339375, 0.999963051871)451.141388356, 676.534353337, 35.755167905
22given(-0.145873955415, 0.989294437182, 0.00416001118191), (-0.989301352797, -0.145880485609, 0.00131044745604), (0.00190328282983, -0.00392434453614, 0.999990488472)44.7018066574, 627.22380306, 39.8066483965

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Components

#1: Protein
Tail Tube of Bacteriophage Goslar


Mass: 32990.105 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Source: (natural) Goslarvirus / References: UniProt: A0A482GE68
#2: Protein
Tail sheath protein gp29 gp29PR domain-containing protein


Mass: 76777.891 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Source: (natural) Goslarvirus / References: UniProt: A0A482GKT6
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Goslarvirus / Type: VIRUS / Entity ID: all / Source: NATURAL
Molecular weightExperimental value: NO
Source (natural)Organism: Goslarvirus
Details of virusEmpty: NO / Enveloped: NO / Isolate: STRAIN / Type: VIRION
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid type: Quantifoil
VitrificationInstrument: FEI VITROBOT MARK II / Cryogen name: ETHANE / Humidity: 95 % / Chamber temperature: 277 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1200 nm
Image recordingElectron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)

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Processing

EM software
IDNameCategory
1cryoSPARCparticle selection
2EPUimage acquisition
4cryoSPARCCTF correction
13cryoSPARC3D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.39 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 47640 / Symmetry type: POINT
RefinementCross valid method: NONE
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Displacement parametersBiso mean: 131.68 Å2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.001593408
ELECTRON MICROSCOPYf_angle_d0.3675127008
ELECTRON MICROSCOPYf_chiral_restr0.03914148
ELECTRON MICROSCOPYf_plane_restr0.003116692
ELECTRON MICROSCOPYf_dihedral_angle_d7.006834116
Refine LS restraints NCS
Ens-IDDom-IDAsym-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2JAPELECTRON MICROSCOPYNCS constraints1.37851268994E-12
ens_1d_3JAPELECTRON MICROSCOPYNCS constraints3.2078009437E-11
ens_1d_4JAPELECTRON MICROSCOPYNCS constraints1.47923211371E-12
ens_1d_5JAPELECTRON MICROSCOPYNCS constraints1.16996162695E-12
ens_1d_6JAPELECTRON MICROSCOPYNCS constraints1.01640102855E-12
ens_1d_7JAPELECTRON MICROSCOPYNCS constraints2.54862996759E-10
ens_1d_8JAPELECTRON MICROSCOPYNCS constraints1.56620880632E-12
ens_1d_9JAPELECTRON MICROSCOPYNCS constraints3.79037485444E-13
ens_1d_10JAPELECTRON MICROSCOPYNCS constraints1.45599144938E-12
ens_1d_11JAPELECTRON MICROSCOPYNCS constraints1.81052925527E-12
ens_1d_12JAPELECTRON MICROSCOPYNCS constraints2.05702322423E-10
ens_2d_2NMELECTRON MICROSCOPYNCS constraints3.08756554428E-12
ens_2d_3NMELECTRON MICROSCOPYNCS constraints1.91220257452E-12
ens_2d_4NMELECTRON MICROSCOPYNCS constraints1.83696727603E-13
ens_2d_5NMELECTRON MICROSCOPYNCS constraints3.13928745759E-11
ens_2d_6NMELECTRON MICROSCOPYNCS constraints1.02318064674E-12
ens_2d_7NMELECTRON MICROSCOPYNCS constraints1.5422223607E-12
ens_2d_8NMELECTRON MICROSCOPYNCS constraints1.15806604567E-12
ens_2d_9NMELECTRON MICROSCOPYNCS constraints4.38088507801E-12
ens_2d_10NMELECTRON MICROSCOPYNCS constraints8.51970732937E-13
ens_2d_11NMELECTRON MICROSCOPYNCS constraints5.61460435578E-12
ens_2d_12NMELECTRON MICROSCOPYNCS constraints1.93919218006E-12

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