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- EMDB-75688: Bacteriophage Goslar Tail Assembly -

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Basic information

Entry
Database: EMDB / ID: EMD-75688
TitleBacteriophage Goslar Tail Assembly
Map data
Sample
  • Virus: Goslarvirus
    • Protein or peptide: Tail Tube of Bacteriophage Goslar
    • Protein or peptide: Tail sheath protein gp29 gp29PR domain-containing protein
KeywordsTail Assembly / VIRAL PROTEIN
Function / homology: / Phage tail tube protein / : / Bacteriophage phiKZ, gp29PR / Uncharacterized protein / Tail sheath protein gp29 gp29PR domain-containing protein
Function and homology information
Biological speciesGoslarvirus
Methodsingle particle reconstruction / cryo EM / Resolution: 3.39 Å
AuthorsBasu D / Gu Y / Corbett KD
Funding support United States, 1 items
OrganizationGrant numberCountry
Howard Hughes Medical Institute (HHMI)Emerging Pathogens Initiative United States
CitationJournal: To Be Published
Title: Bacteriophage Goslar Tail Assembly
Authors: Basu D / Gu Y / Corbett KD
History
DepositionFeb 24, 2026-
Header (metadata) releaseAug 19, 2026-
Map releaseAug 19, 2026-
UpdateAug 19, 2026-
Current statusAug 19, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_75688.png

Downloads & links

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Map

FileDownload / File: emd_75688.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.4 Å/pix.
x 420 pix.
= 588. Å		1.4 Å/pix.
x 420 pix.
= 588. Å		1.4 Å/pix.
x 420 pix.
= 588. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.4 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.25
Minimum - Maximum-0.2786223 - 1.031038
Average (Standard dev.)0.00792015 (±0.06994646)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions420420420
Spacing420420420
CellA=B=C: 588.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_75688_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_75688_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Goslarvirus

EntireName: Goslarvirus
Components
  • Virus: Goslarvirus
    • Protein or peptide: Tail Tube of Bacteriophage Goslar
    • Protein or peptide: Tail sheath protein gp29 gp29PR domain-containing protein

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Supramolecule #1: Goslarvirus

SupramoleculeName: Goslarvirus / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / NCBI-ID: 2733105 / Sci species name: Goslarvirus / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No

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Macromolecule #1: Tail Tube of Bacteriophage Goslar

MacromoleculeName: Tail Tube of Bacteriophage Goslar / type: protein_or_peptide / ID: 1 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Goslarvirus
Molecular weightTheoretical: 32.990105 KDa
SequenceString: MRPDNTLLGE QGVGALSNAA MVDIRAGAQN GYISNIGTYV ANANYIPNQM FCLLLEAPRG FNYLPNPDVQ IGYLKALVEE VAHSITGLQ RGLEVEFVSV PVSGSGEIQE EVSDVKRPRS NPTFGIYEKE GRSVSYFLEQ WITYLLMDPD AKYPMLSSIV S TGGPTDLL ...String:
MRPDNTLLGE QGVGALSNAA MVDIRAGAQN GYISNIGTYV ANANYIPNQM FCLLLEAPRG FNYLPNPDVQ IGYLKALVEE VAHSITGLQ RGLEVEFVSV PVSGSGEIQE EVSDVKRPRS NPTFGIYEKE GRSVSYFLEQ WITYLLMDPD AKYPMLSSIV S TGGPTDLL ADYRSATMLF VEPDRQHKKV VNAWLCTNMM PHGTGDWTSR RNKNDAPNVV ELSIQFTALT QTNYGVRAFA QR LLDKMNL LKVNPDFRPA YLSDIDPAVA AQKVGYTFDF DNNDTFEVPN TYQNVTPRV

UniProtKB: Uncharacterized protein

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Macromolecule #2: Tail sheath protein gp29 gp29PR domain-containing protein

MacromoleculeName: Tail sheath protein gp29 gp29PR domain-containing protein
type: protein_or_peptide / ID: 2 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Goslarvirus
Molecular weightTheoretical: 76.777891 KDa
SequenceString: MRLKNSTPRA IFTGFKEGLT TDPVTAPEIT PIHLPYVFIQ GGRGKEDTLL LTGDALSTVY GEDMLNYRSK YASPATLIAR QAASTGSAI FTKRLVAPDA TAARIRIGVE VVADKLVVYQ RNADGSFVKD TLGNKIPDGD KTVDGLKMRW VVNHDKDAET P NAFGKDEV ...String:
MRLKNSTPRA IFTGFKEGLT TDPVTAPEIT PIHLPYVFIQ GGRGKEDTLL LTGDALSTVY GEDMLNYRSK YASPATLIAR QAASTGSAI FTKRLVAPDA TAARIRIGVE VVADKLVVYQ RNADGSFVKD TLGNKIPDGD KTVDGLKMRW VVNHDKDAET P NAFGKDEV VLGQLTATGG AQSNYYPILD GLVSWRGAEG DNIGIRLEAP TALSSNPTRT DIIERIGAFL YRIQFVERKS SR TAPTVIR TISGMEQETF ALQEGVIQPD DETDLSFGKV VVDAYEYNET GNTPIFAPMD QMHLYQKNID DVVKMLYESE RKV NNDLLT DVEFVEGQMN IFTGVDYNGI PYQTIEVLDA DQGGAIFDGT STFYAIDGSD GDVNWDTFDA LAKQQFESFG NMGE DLEDM AFYPFSIVYD IGYKVDTKKA MANVLSLRPD VMIIASTHTW KGVELNVDEE SSMGAMLRNY YRLHPESVLY NTPAC RAAV FMQYGESIEA PSLGKVPLTL EVANKLATYM GASDGVIRGT RIDNNPGNVV TTMRKINKTY RNATVRDRDW ENGLNY VQT YNRNSNFFPA WGTIYNNDRS VWRSLINVII GCDLTRVCYR LWAETTGTTG MDETLFLKLM GERFLEYTTG RYNDHVT VK GRFYLDKEDG YNGFSYHGDI SLIGDPGMTV GTFTITGLRR NTEAAEAA

UniProtKB: Tail sheath protein gp29 gp29PR domain-containing protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
GridModel: Quantifoil
VitrificationCryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK II

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.39 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 47640
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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