National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)
1R01GM124348-01
United States
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)
1R01GM123089
United States
American Heart Association
18POST34030308
United States
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)
R01GM097194
United States
German Research Foundation (DFG)
AR1164/1-1
Germany
Citation
Journal: J Cell Biol / Year: 2018 Title: Cryo-electron microscopy structure of the lipid droplet-formation protein seipin. Authors: Xuewu Sui / Henning Arlt / Kelly P Brock / Zon Weng Lai / Frank DiMaio / Debora S Marks / Maofu Liao / Robert V Farese / Tobias C Walther / Abstract: Metabolic energy is stored in cells primarily as triacylglycerols in lipid droplets (LDs), and LD dysregulation leads to metabolic diseases. The formation of monolayer-bound LDs from the endoplasmic ...Metabolic energy is stored in cells primarily as triacylglycerols in lipid droplets (LDs), and LD dysregulation leads to metabolic diseases. The formation of monolayer-bound LDs from the endoplasmic reticulum (ER) bilayer is poorly understood, but the ER protein seipin is essential to this process. In this study, we report a cryo-electron microscopy structure and functional characterization of seipin. The structure reveals a ring-shaped dodecamer with the luminal domain of each monomer resolved at ∼4.0 Å. Each luminal domain monomer exhibits two distinctive features: a hydrophobic helix (HH) positioned toward the ER bilayer and a β-sandwich domain with structural similarity to lipid-binding proteins. This structure and our functional testing in cells suggest a model in which seipin oligomers initially detect forming LDs in the ER via HHs and subsequently act as membrane anchors to enable lipid transfer and LD growth.
History
Deposition
Sep 28, 2018
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Header (metadata) release
Oct 17, 2018
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Map release
Oct 17, 2018
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Update
Dec 18, 2019
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Current status
Dec 18, 2019
Processing site: RCSB / Status: Released
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Structure visualization
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Surface view with section colored by density value
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