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Yorodumi- EMDB-7821: CryoEM structure of Tetrahymena telomerase with telomeric DNA at ... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-7821 | ||||||||||||||||||||||||||||||
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Title | CryoEM structure of Tetrahymena telomerase with telomeric DNA at 4.8 Angstrom resolution | ||||||||||||||||||||||||||||||
Map data | Tetrahymena telomerase with telomeric DNA | ||||||||||||||||||||||||||||||
Sample |
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Keywords | Telomerase / telomere / REPLICATION | ||||||||||||||||||||||||||||||
Function / homology | Function and homology information telomerase catalytic core complex assembly / telomerase RNA stabilization / telomerase catalytic core complex / : / DNA replication factor A complex / single-stranded telomeric DNA binding / telomerase RNA binding / telomerase holoenzyme complex / telomeric DNA binding / telomere maintenance via telomerase ...telomerase catalytic core complex assembly / telomerase RNA stabilization / telomerase catalytic core complex / : / DNA replication factor A complex / single-stranded telomeric DNA binding / telomerase RNA binding / telomerase holoenzyme complex / telomeric DNA binding / telomere maintenance via telomerase / RNA-directed DNA polymerase / DNA recombination / DNA replication / chromosome, telomeric region / DNA repair / DNA binding / zinc ion binding / metal ion binding Similarity search - Function | ||||||||||||||||||||||||||||||
Biological species | Tetrahymena thermophila (eukaryote) | ||||||||||||||||||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.8 Å | ||||||||||||||||||||||||||||||
Authors | Jiang J / Wang Y / Susac L / Chan H / Basu R / Zhou ZH / Feigon J | ||||||||||||||||||||||||||||||
Funding support | United States, 9 items
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Citation | Journal: Cell / Year: 2018 Title: Structure of Telomerase with Telomeric DNA. Authors: Jiansen Jiang / Yaqiang Wang / Lukas Sušac / Henry Chan / Ritwika Basu / Z Hong Zhou / Juli Feigon / Abstract: Telomerase is an RNA-protein complex (RNP) that extends telomeric DNA at the 3' ends of chromosomes using its telomerase reverse transcriptase (TERT) and integral template-containing telomerase RNA ...Telomerase is an RNA-protein complex (RNP) that extends telomeric DNA at the 3' ends of chromosomes using its telomerase reverse transcriptase (TERT) and integral template-containing telomerase RNA (TER). Its activity is a critical determinant of human health, affecting aging, cancer, and stem cell renewal. Lack of atomic models of telomerase, particularly one with DNA bound, has limited our mechanistic understanding of telomeric DNA repeat synthesis. We report the 4.8 Å resolution cryoelectron microscopy structure of active Tetrahymena telomerase bound to telomeric DNA. The catalytic core is an intricately interlocked structure of TERT and TER, including a previously structurally uncharacterized TERT domain that interacts with the TEN domain to physically enclose TER and regulate activity. This complete structure of a telomerase catalytic core and its interactions with telomeric DNA from the template to telomere-interacting p50-TEB complex provides unanticipated insights into telomerase assembly and catalytic cycle and a new paradigm for a reverse transcriptase RNP. | ||||||||||||||||||||||||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_7821.map.gz | 58.5 MB | EMDB map data format | |
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Header (meta data) | emd-7821-v30.xml emd-7821.xml | 21.8 KB 21.8 KB | Display Display | EMDB header |
Images | emd_7821.png | 153.8 KB | ||
Masks | emd_7821_msk_1.map | 64 MB | Mask map | |
Filedesc metadata | emd-7821.cif.gz | 7.5 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-7821 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-7821 | HTTPS FTP |
-Validation report
Summary document | emd_7821_validation.pdf.gz | 542 KB | Display | EMDB validaton report |
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Full document | emd_7821_full_validation.pdf.gz | 541.6 KB | Display | |
Data in XML | emd_7821_validation.xml.gz | 6 KB | Display | |
Data in CIF | emd_7821_validation.cif.gz | 6.9 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-7821 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-7821 | HTTPS FTP |
-Related structure data
Related structure data | 6d6vMC 7820C C: citing same article (ref.) M: atomic model generated by this map |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_7821.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Tetrahymena telomerase with telomeric DNA | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.36 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
File | emd_7821_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
+Entire : Endogenous assembled Tetrahymena telomerase bound with telomeric DNA
+Supramolecule #1: Endogenous assembled Tetrahymena telomerase bound with telomeric DNA
+Macromolecule #1: Telomerase reverse transcriptase
+Macromolecule #2: Telomerase-associated protein 82
+Macromolecule #3: Telomerase holoenzyme TEB heterotrimer Teb3 subunit
+Macromolecule #4: Telomerase holoenzyme Teb2 subunit
+Macromolecule #7: Telomerase-associated protein 50
+Macromolecule #8: Telomerase associated protein p65
+Macromolecule #5: RNA (159-MER)
+Macromolecule #6: DNA (5'-D(P*GP*TP*TP*GP*GP*GP*GP*TP*TP*GP*GP*GP*GP*TP*TP*GP*GP*GP...
+Macromolecule #9: ZINC ION
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average exposure time: 12.0 sec. / Average electron dose: 52.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: EMDB MAP EMDB ID: |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 4.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 52506 |
Initial angle assignment | Type: PROJECTION MATCHING / Software - Name: RELION |
Final angle assignment | Type: PROJECTION MATCHING / Software - Name: RELION |