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Yorodumi- EMDB-6976: Structure of the Herpes simplex virus type 2 C-capsid with capsid... -
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-Basic information
Entry | Database: EMDB / ID: EMD-6976 | |||||||||
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Title | Structure of the Herpes simplex virus type 2 C-capsid with capsid-vertex-specific component | |||||||||
Map data | full_map | |||||||||
Sample |
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Function / homology | Function and homology information chromosome organization => GO:0051276 / T=16 icosahedral viral capsid / deNEDDylase activity / viral genome packaging / viral tegument / viral capsid assembly / viral DNA genome replication / viral release from host cell / viral process / viral penetration into host nucleus ...chromosome organization => GO:0051276 / T=16 icosahedral viral capsid / deNEDDylase activity / viral genome packaging / viral tegument / viral capsid assembly / viral DNA genome replication / viral release from host cell / viral process / viral penetration into host nucleus / viral capsid / symbiont-mediated perturbation of host ubiquitin-like protein modification / ubiquitinyl hydrolase 1 / host cell cytoplasm / cysteine-type deubiquitinase activity / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / symbiont entry into host cell / host cell nucleus / structural molecule activity / DNA binding Similarity search - Function | |||||||||
Biological species | Human herpesvirus 2 | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.75 Å | |||||||||
Authors | Wang JL / Yuan S / Zhu DJ / Tang H / Wang N / Chen WY / Gao Q / Li YH / Wang JZ / Liu HR ...Wang JL / Yuan S / Zhu DJ / Tang H / Wang N / Chen WY / Gao Q / Li YH / Wang JZ / Liu HR / Zhang XZ / Rao ZH / Wang XX | |||||||||
Citation | Journal: Nat Commun / Year: 2018 Title: Structure of the herpes simplex virus type 2 C-capsid with capsid-vertex-specific component. Authors: Jialing Wang / Shuai Yuan / Dongjie Zhu / Hao Tang / Nan Wang / Wenyuan Chen / Qiang Gao / Yuhua Li / Junzhi Wang / Hongrong Liu / Xinzheng Zhang / Zihe Rao / Xiangxi Wang / Abstract: Herpes simplex viruses (HSVs) cause human oral and genital ulcer diseases. Patients with HSV-2 have a higher risk of acquiring a human immunodeficiency virus infection. HSV-2 is a member of the α- ...Herpes simplex viruses (HSVs) cause human oral and genital ulcer diseases. Patients with HSV-2 have a higher risk of acquiring a human immunodeficiency virus infection. HSV-2 is a member of the α-herpesvirinae subfamily that together with the β- and γ-herpesvirinae subfamilies forms the Herpesviridae family. Here, we report the cryo-electron microscopy structure of the HSV-2 C-capsid with capsid-vertex-specific component (CVSC) that was determined at 3.75 Å using a block-based reconstruction strategy. We present atomic models of multiple conformers for the capsid proteins (VP5, VP23, VP19C, and VP26) and CVSC. Comparison of the HSV-2 homologs yields information about structural similarities and differences between the three herpesviruses sub-families and we identify α-herpesvirus-specific structural features. The hetero-pentameric CVSC, consisting of a UL17 monomer, a UL25 dimer and a UL36 dimer, is bound tightly by a five-helix bundle that forms extensive networks of subunit contacts with surrounding capsid proteins, which reinforce capsid stability. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_6976.map.gz | 1.4 GB | EMDB map data format | |
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Header (meta data) | emd-6976-v30.xml emd-6976.xml | 21.5 KB 21.5 KB | Display Display | EMDB header |
Images | emd_6976.png | 328.9 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-6976 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-6976 | HTTPS FTP |
-Related structure data
Related structure data | 5zz8MC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_6976.map.gz / Format: CCP4 / Size: 6.4 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | full_map | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.38 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Human herpesvirus 2
Entire | Name: Human herpesvirus 2 |
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Components |
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-Supramolecule #1: Human herpesvirus 2
Supramolecule | Name: Human herpesvirus 2 / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / NCBI-ID: 10310 / Sci species name: Human herpesvirus 2 / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: Yes |
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-Macromolecule #1: VP19C
Macromolecule | Name: VP19C / type: protein_or_peptide / ID: 1 / Number of copies: 5 / Enantiomer: LEVO |
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Source (natural) | Organism: Human herpesvirus 2 |
Molecular weight | Theoretical: 50.566648 KDa |
Recombinant expression | Organism: Chlorocebus sabaeus (green monkey) |
Sequence | String: MKTKPLPTAP MAWAESAVET TTSPRELAGH APLRRVLRPP IARRDGPVLL GDRAPRRTAS TMWLLGIDPA ESSPGTRATR DDTEQAVDK ILRGARRAGG LTVPGAPRYH LTRQVTLTDL CQPNAERAGA LLLALRHPTD LPHLARHRAP PGRQTERLAE A WGQLLEAS ...String: MKTKPLPTAP MAWAESAVET TTSPRELAGH APLRRVLRPP IARRDGPVLL GDRAPRRTAS TMWLLGIDPA ESSPGTRATR DDTEQAVDK ILRGARRAGG LTVPGAPRYH LTRQVTLTDL CQPNAERAGA LLLALRHPTD LPHLARHRAP PGRQTERLAE A WGQLLEAS ALGSGRAESG CARAGLVSFN FLVAACAAAY DARDAAEAVR AHITTNYGGT RAGARLDRFS ECLRAMVHTH VF PHEVMRF FGGLVSWVTQ DELASVTAVC SGPQEATHTG HPGRPRSAVT IPACAFVDLD AELCLGGPGA AFLYLVFTYR QCR DQELCC VYVVKSQLPP RGLEAALERL FGRLRITNTI HGAEDMTPPP PNRNVDFPLA VLAASSQSPR CSASQVTNPQ FVDR LYRWQ PDLRGRPTAR TCTYAAFAEL GVMPDDSPRC LHRTERFGAV GVPVVILEGV VWRPGGWRAC A |
-Macromolecule #2: VP23
Macromolecule | Name: VP23 / type: protein_or_peptide / ID: 2 / Number of copies: 10 / Enantiomer: LEVO |
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Source (natural) | Organism: Human herpesvirus 2 |
Molecular weight | Theoretical: 34.373785 KDa |
Recombinant expression | Organism: Chlorocebus sabaeus (green monkey) |
Sequence | String: MITDCFEADI AIPSGISRPD AAALQRCEGR VVFLPTIRRQ LALADVAHES FVSGGVSPDT LGLLLAYRRR FPAVITRVLP TRIVACPVD LGLTHAGTVN LRNTSPVDLC NGDPVSLVPP VFEGQATDVR LESLDLTLRF PVPLPTPLAR EIVARLVARG I RDLNPDPR ...String: MITDCFEADI AIPSGISRPD AAALQRCEGR VVFLPTIRRQ LALADVAHES FVSGGVSPDT LGLLLAYRRR FPAVITRVLP TRIVACPVD LGLTHAGTVN LRNTSPVDLC NGDPVSLVPP VFEGQATDVR LESLDLTLRF PVPLPTPLAR EIVARLVARG I RDLNPDPR TPGELPDLNV LYYNGARLSL VADVQQLASV NTELRSLVLN MVYSITEGTT LILTLIPRLL ALSAQDGYVN AL LQMQSVT REAAQLIHPE APMLMQDGER RLPLYEALVA WLAHAGQLGD ILALAPAVRV CTFDGAAVVQ SGDMAPVIRY P |
-Macromolecule #3: Major capsid protein
Macromolecule | Name: Major capsid protein / type: protein_or_peptide / ID: 3 / Number of copies: 16 / Enantiomer: LEVO |
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Source (natural) | Organism: Human herpesvirus 2 |
Molecular weight | Theoretical: 149.384375 KDa |
Recombinant expression | Organism: Chlorocebus sabaeus (green monkey) |
Sequence | String: MAAPARDPPG YRYAAAMVPT GSILSTIEVA SHRRLFDFFA RVRSDENSLY DVEFDALLGS YCNTLSLVRF LELGLSVACV CTKFPELAY MNEGRVQFEV HQPLIARDGP HPVEQPVHNY MTKVIDRRAL NAAFSLATEA IALLTGEALD GTGISLHRQL R AIQQLARN ...String: MAAPARDPPG YRYAAAMVPT GSILSTIEVA SHRRLFDFFA RVRSDENSLY DVEFDALLGS YCNTLSLVRF LELGLSVACV CTKFPELAY MNEGRVQFEV HQPLIARDGP HPVEQPVHNY MTKVIDRRAL NAAFSLATEA IALLTGEALD GTGISLHRQL R AIQQLARN VQAVLGAFER GTADQMLHVL LEKAPPLALL LPMQRYLDNG RLATRVARAT LVAELKRSFC DTSFFLGKAG HR REAIEAW LVDLTTATQP SVAVPRLTHA DTRGRPVDGV LVTTAAIKQR LLQSFLKVED TEADVPVTYG EMVLNGANLV TAL VMGKAV RSLDDVGRHL LEMQEEQLEA NRETLDELES APQTTRVRAD LVAIGDRLVF LEALEKRIYA ATNVPYPLVG AMDL TFVLP LGLFNPAMER FAAHAGDLVP APGHPEPRAF PPRQLFFWGK DHQVLRLSME NAVGTVCHPS LMNIDAAVGG VNHDP VEAA NPYGAYVAAP AGPGADMQQR FLNAWRQRLA HGRVRWVAEC QMTAEQFMQP DNANLALELH PAFDFFAGVA DVELPG GEV PPAGPGAIQA TWRVVNGNLP LALCPVAFRD ARGLELGVGR HAMAPATIAA VRGAFEDRSY PAVFYLLQAA IHGSEHV FC ALARLVTQCI TSYWNNTRCA AFVNDYSLVS YIVTYLGGDL PEECMAVYRD LVAHVEALAQ LVDDFTLPGP ELGGQAQA E LNHLMRDPAL LPPLVWDCDG LMRHAALDRH RDCRIDAGGH EPVYAAACNV ATADFNRNDG RLLHNTQARA ADAADDRPH RPADWTVHHK IYYYVLVPAF SRGRCCTAGV RFDRVYATLQ NMVVPEIAPG EECPSDPVTD PAHPLHPANL VANTVNAMFH NGRVVVDGP AMLTLQVLAH NMAERTTALL CSAAPDAGAN TASTANMRIF DGALHAGVLL MAPQHLDHTI QNGEYFYVLP V HALFAGAD HVANAPNFPP ALRDLARHVP LVPPALGANY FSSIRQPVVQ HARESAAGEN ALTYALMAGY FKMSPVALYH QL KTGLHPG FGFTVVRQDR FVTENVLFSE RASEAYFLGQ LQVARHETGG GVNFTLTQPR GNVDLGVGYT AVAATATVRN PVT DMGNLP QNFYLGRGAP PLLDNAAAVY LRNAVVAGNR LGPAQPLPVF GCAQVPRRAG MDHGQDAVCE FIATPVATDI NYFR RPCNP RGRAAGGVYA GDKEGDVIAL MYDHGQSDPA RPFAATANPW ASQRFSYGDL LYNGAYHLNG ASPVLSPCFK FFTAA DITA KHRCLERLIV ETGSAVSTAT AASDVQFKRP PGCRELVEDP CGLFQEAYPI TCASDPALLR SARDGEAHAR ETHFTQ YLI YDASPLKGLS L |
-Macromolecule #4: VP26
Macromolecule | Name: VP26 / type: protein_or_peptide / ID: 4 / Number of copies: 15 / Enantiomer: LEVO |
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Source (natural) | Organism: Human herpesvirus 2 |
Molecular weight | Theoretical: 12.147707 KDa |
Recombinant expression | Organism: Chlorocebus sabaeus (green monkey) |
Sequence | String: MAAPQFHRPS TITADNVRAL GMRGLVLATN NAQFIMDNSY PHPHGTQGAV REFLRGQAAA LTDLGVTHAN NTFAPQPMFA GDAAAEWLR PSFGLKRTYS PFVVRDPKTP STP |
-Macromolecule #5: UL17
Macromolecule | Name: UL17 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Human herpesvirus 2 |
Molecular weight | Theoretical: 74.747805 KDa |
Recombinant expression | Organism: Chlorocebus sabaeus (green monkey) |
Sequence | String: MNAHFANEVQ YDLTRDPSSP ASLIHVIISS ECLAAAGVPL SALVRGRPDG GAAANFRVET QTRAHATGDC TPWRSAFAAY VPADAVGAI LAPVIPAHPD LLPRVPSAGG LFVSLPVACD AQGVYDPYTV AALRLAWGPW ATCARVLLFS YDELVPPNTR Y AADGARLM ...String: MNAHFANEVQ YDLTRDPSSP ASLIHVIISS ECLAAAGVPL SALVRGRPDG GAAANFRVET QTRAHATGDC TPWRSAFAAY VPADAVGAI LAPVIPAHPD LLPRVPSAGG LFVSLPVACD AQGVYDPYTV AALRLAWGPW ATCARVLLFS YDELVPPNTR Y AADGARLM RLCRHFCRYV ARLGAAAPAA ATEAAAHLSL GMGESGTPTP QASSVSGGAG PAVVGTPDPP ISPEEQLTAP GG DTATAED VSITQENEEI LALVQRAVQD VTRRHPVRAR PKHAASGVAS GLRQGALVHQ AVSGGALGAS DAEAVLAGLE PPG GGRFAT PGGPRAAGED VLNDVLTLVP GTAKPRSLVE WLDRGWEALA GGDRPDWLWS RRSISVVLRH HYGTKQRFVV VSYE NSVAW GGRRARPPRL SSELATALTE ACAAERVVRP HQLSPAAQTA LLRRFPALEG PLRHPRPVLQ PFDIAAEVAF VARIQ IACL RALGHSIRAA LQGGPRIFQR LRYDFGPHQS EWLGEVTRRF PVLLENLMRA LEGTAPDAFF HTAYALAVLA HLGGQG GRG RRRRLVPLSD DIPARFADSD AHYAFDYYST SGDTLRLTNR PIAVVIDGDV NGREQSKCRF MEGSPSTAPH RVCEQYL PG ESYAYLCLGF NRRLCGLVVF PGGFAFTINT AAYLSLADPV ARAVGLRFCR GAATGPGLVR |
-Macromolecule #6: UL25
Macromolecule | Name: UL25 / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: Human herpesvirus 2 |
Molecular weight | Theoretical: 63.623836 KDa |
Recombinant expression | Organism: Chlorocebus sabaeus (green monkey) |
Sequence | String: MDPYYPFDAL DVWEHRRFIV ADSRSFITPE FPRDFWMLPV FNIPRETAAE RAAVLQAQRT AAAAALENAA LQAAELPVDI ERRIRPIEQ QVHHIADALE ALETAAAAAE EADAARDAEA RGEGAADGAA PSPTAGPAAA EMEVQIVRND PPLRYDTNLP V DLLHMVYA ...String: MDPYYPFDAL DVWEHRRFIV ADSRSFITPE FPRDFWMLPV FNIPRETAAE RAAVLQAQRT AAAAALENAA LQAAELPVDI ERRIRPIEQ QVHHIADALE ALETAAAAAE EADAARDAEA RGEGAADGAA PSPTAGPAAA EMEVQIVRND PPLRYDTNLP V DLLHMVYA GRGAAGSSGV VFGTWYRTIQ ERTIADFPLT TRSADFRDGR MSKTFMTALV LSLQSCGRLY VGQRHYSAFE CA VLCLYLL YRTTHESSPD RDRAPVAFGD LLARLPRYLA RLAAVIGDES GRPQYRYRDD KLPKAQFAAA GGRYEHGALA THV VIATLV RHGVLPAAPG DVPRDTSTRV NPDDVAHRDD VNRAAAAFLA RGHNLFLWED QTLLRATANT ITALAVLRRL LANG NVYAD RLDNRLQLGM LIPGAVPAEA IARGASGLDS GAIKSGDNNL EALCVNYVLP LYQADPTVEL TQLFPGLAAL CLDAQ AGRP LASTRRVVDM SSGARQAALV RLTALELINR TRTNTTPVGE IINAHDALGI QYEQGLGLLA QQARIGLASN AKRFAT FNV GSDYDLLYFL CLGFIPQYLS VA |
-Macromolecule #7: UL36
Macromolecule | Name: UL36 / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: Human herpesvirus 2 |
Molecular weight | Theoretical: 330.616156 KDa |
Recombinant expression | Organism: Chlorocebus sabaeus (green monkey) |
Sequence | String: MIPAALPHPT MKRQGDRDIV VTGVRNQFAT DLEPGGSVSC MRSSLSFLSL LFDVGPRDVL SAEAIEGCLV EGGEWTRAAA GSGPPRMCS IIELPNFLEY PAARGGLRCV FSRVYGEVGF FGEPTAGLLE TQCPAHTFFA GPWAMRPLSY TLLTIGPLGM G LYRDGDTA ...String: MIPAALPHPT MKRQGDRDIV VTGVRNQFAT DLEPGGSVSC MRSSLSFLSL LFDVGPRDVL SAEAIEGCLV EGGEWTRAAA GSGPPRMCS IIELPNFLEY PAARGGLRCV FSRVYGEVGF FGEPTAGLLE TQCPAHTFFA GPWAMRPLSY TLLTIGPLGM G LYRDGDTA YLFDPHGLPA GTPAFIAKVR AGDVYPYLTY YAHDRPKVRW AGAMVFFVPS GPGAVAPADL TAAALHLYGA SE TYLQDEP FVERRVAITH PLRGEIGGLG ALFVGVVPRG DGEGSGPVVP ALPAPTHVQT PRADRPPEAP RGASGPPDTP QAG HPNRPP DDVWAAALEG TPPAKPSAPD AAASGPPHAA PPPQTPAGDA AEEAEDLRVL EVGAVPVGRH RARYSTGLPK RRRP TWTPP SSVEDLTSGE RPAPKAPPAK AKKKSAPKKK APVAAEVPAS SPTPIAATVP PAPDTPPQSG QGGGDDGPAS PSSPS VLET LGARRPPEPP GADLAQLFEV HPNVAATAVR LAARDAALAR EVAACSQLTI NALRSPYPAH PGLLELCVIF FFERVL AFL IENGARTHTQ AGVAGPAAAL LDFTLRMLPR KTAVGDFLAS TRMSLADVAA HRPLIQHLLD ENSQIGRLAL AKLVLVA RD VIRETDAFYG DLADLDLQLR AAPPANLYAR LGEWLLERSR AHPNTLFAPA TPTHPEPLLH RIQALAQFAR GEEMRVEA E AREMREALDA LARGVDSVSQ RAGPLTVMPV PAAPGAGGRA PCPPALGPEA IQARLEDVRI QARRAIESAI KEYFHRGAV YSAKALQASD SHDCRFHVAS AAVVPMVQLL ESLPAFDQHT RDVAQRAALP PPPPLATSPQ AILLRDLLQR GQTLDAPEDL AAWLSVLTD AATQGLIERK PLEELARSIH GINDQQARRS SGLAELQRFD ALDAALAQQL DSDAAFVPAT GPAPYVDGGG L SPEATRMA EDALRQARAM EAAKMTAELA PEARSRLRER AHALEAMLND ARERAKVAHD AREKFLHKLQ GVLRPLPDFV GL KACPAVL ATLRASLPAG WTDLADAVRG PPPEVTAALR ADLWGLLGQY REALEHPTPD TATALAGLHP AFVVVLKTLF ADA PETPVL VQFFSDHAPT IAKAVSNAIN AGSAAVATAS PAATVDAAVR AHGALADAVS ALGAAARDPA SPLSFLAALA DSAA GYVKA TRLALEARGA IDELTTLGSA AADLVVQARR ACAQPEGDHA ALIDAAARAT TAARESLAGH EAGFGGLLHA EGTAG DHSP SGRALQELGK VIGATRRRAD ELEAAVADLT AKMAAQRARG SSERWAAGVE AALDRVENRA EFDVVELRRL QALAGT HGY NPRDFRKRAE QALAANAEAV TLALDTAFAF NPYTPENQRH PMLPPLAAIH RLGWSAAFHA AAETYADMFR VDAEPLA RL LRIAEGLLEM AQAGDGFIDY HEAVGRLADD MTSVPGLRRY VPFFQHGYAD YVELRDRLDA IRADVHRALG GVPLDLAA A AEQISAARND PEATAELVRT GVTLPCPSED ALVACAAALE RVDQSPVKNT AYAEYVAFVT RQDTAETKDA VVRAKQQRA EATERVMAGL REALAARERR AQIEAEGLAN LKTMLKVVAV PATVAKTLDQ ARSVAEIADQ VEVLLDQTEK TRELDVPAVI WLEHAQRTF ETHPLSAARG DGPGPLARHA GRLGALFDTR RRVDALRRSL EEAEAEWDEV WGRFGRVRGG AWKSPEGFRA M HEQLRALQ DTTNTVSGLR AQPAYERLSA RYQGVLGAKG AERAEAVEEL GARVTKHTAL CARLRDEVVR RVPWEMNFDA LG RLLAEFD AAAADLAPWA VEEFRGAREL IQYRMGLYSA YARAGGQTGA GAESAPAPLL VDLRALDARA RASSSPEGHE VDP QLLRRR GEAYLRAGGD PGPLVLREAV SALDLPFATS FLAPDGTPLQ YALCFPAVTD KLGALLMRPE AACVRPPLPT DVLE SAPTV TAMYVLTVVN RLQLALSDAQ AANFQLFGRF VRHRQATWGA SMDAAAELYV ALVATTLTRE FGCRWAQLGW ASGAA APRP PPGPRGSQRH CVAFNENDVL VALVAGVPEH IYNFWRLDLV RQHEYMHLTL ERAFEDAAES MLFIQRLTPH PDARIR VLP TFLDGGPPTR GLLFGTRLAD WRRGKLSETD PLAPWRSALE LGTQRRDAPA LGKLSPAQAL AAVSVLGRMC LPSAALA AL WTCMFPDDYT EYDSFDALLA ARLESGQTLG PAGGREASLP EAPHALYRPT GQHVAVLAAA THRTPAARVT AMDLVLAA V LLGAPVVVAL RNTTAFSRES ELELCLTLFD SRPGGPDAAL RDVVSSDIET WAVGLLHTDL NPIENACLAA QLPRLSALI AERPLADGPP CLVLVDISMT PVAVLWEAPE PPGPPDVRFV GSEATEELPF VATAGDVLAA SAADADPFFA RAILGRPFDA SLLTGELFP GHPVYQRPLA DEAGPSAPTA ARDPRDLAGG DGGSGPEDPA APPARQADPG VLAPTLLTDA TTGEPVPPRM W AWIHGLEE LASDDAGGPT PNPAPALLPP PATDQSVPTS QYAPRPIGPA ATARETRPSV PPQQNTGRVP VAPRDDPRPS PP TPSPPAD AALPPPAFSG SAAAFSAAVP RVRRSRRTRA KSRAPRASAP PEGWRPPALP APVAPVAASA RPPDQPPTPE SAP PAWVSA LPLPPGPASA RGAFPAPTLA PIPPPPAEGA VVPGGDRRRG RRQTTAGPSP TPPRGPAAGP PRRLTRPAVA SLSA SLNSL PSPRDPADHA AAVSAAAAAV PPSPGLAPPT SAVQTSPPPL APGPVAPSEP LCGWVVPGGP VARRPPPQSP ATKPA ARTR IRARSVPQPP LPQPPLPQPP LPQPPLPQPP LPQPPLPQPP LPQPPLPQPP LPQPPLPQPP LPPVTRTLTP QSRDSV PTP ESPTHTNTHL PVSAVTSWAS SLALHVDSAP PPASLLQTLH ISSDDEHSDA DSLRFSDSDD TEALDPLPPE PHLPPAD EP PGPLAADHLQ SPHSQFGPLP VQANAVLSRR YVRSTGRSAL AVLIRACRRI QQQLQRTRRA LFQRSNAVLT SLHHVRML L G |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 30.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: OTHER |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.75 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 56901 |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |