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- EMDB-69549: Cryo-EM structure of the dimeric WDR11-FAM91A1-C17orf75 complex -

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Basic information

Entry
Database: EMDB / ID: EMD-69549
TitleCryo-EM structure of the dimeric WDR11-FAM91A1-C17orf75 complex
Map data
Sample
  • Complex: dimeric WDR11-FAM91A1-C17orf75 complex
    • Protein or peptide: WD repeat-containing protein 11
    • Protein or peptide: Protein Njmu-R1
    • Protein or peptide: Protein FAM91A1
Keywordsdimeric WDR11-FAM91A1-C17orf75 complex / PROTEIN TRANSPORT
Function / homology
Function and homology information


: / head development / regulation of smoothened signaling pathway / RHOH GTPase cycle / cilium assembly / axoneme / multicellular organism growth / centriole / sperm end piece / sperm principal piece ...: / head development / regulation of smoothened signaling pathway / RHOH GTPase cycle / cilium assembly / axoneme / multicellular organism growth / centriole / sperm end piece / sperm principal piece / trans-Golgi network / intracellular protein transport / heart development / microtubule cytoskeleton / cytoplasmic vesicle / cilium / ciliary basal body / lysosomal membrane / membrane / nucleus / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Protein Njmu-R1 / Njmu-R1-like protein family / WD repeat-containing protein 11 / : / : / : / WDR11 first beta-propeller / WDR11 second beta-propeller / WDR11 TPR domain / FAM91, N-terminal domain ...Protein Njmu-R1 / Njmu-R1-like protein family / WD repeat-containing protein 11 / : / : / : / WDR11 first beta-propeller / WDR11 second beta-propeller / WDR11 TPR domain / FAM91, N-terminal domain / FAM91, C-terminal domain / FAM91 / FAM91 N-terminus / FAM91 C-terminus / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Protein FAM91A1 / WD repeat-containing protein 11 / Protein Njmu-R1
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.07 Å
AuthorsLiu ZM / Zhang YF / Chen ZY / Chen ZG / Ding JP
Funding support China, 4 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32071190 China
National Natural Science Foundation of China (NSFC)32571391 China
Ministry of Science and Technology (MoST, China)2020YFA0509000 China
Ministry of Science and Technology (MoST, China)2020YFA0803200 China
CitationJournal: Structure / Year: 2026
Title: Cryo-EM structure of the dimeric WDR11-FAM91A1-C17orf75 complex (focused on body1)
Authors: Liu ZM / Zhang YF / Chen ZY / Chen ZG / Ding JP
History
DepositionMar 4, 2026-
Header (metadata) releaseJul 29, 2026-
Map releaseJul 29, 2026-
UpdateJul 29, 2026-
Current statusJul 29, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_69549.png

Downloads & links

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Map

FileDownload / File: emd_69549.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.93 Å/pix.
x 600 pix.
= 559.2 Å		0.93 Å/pix.
x 600 pix.
= 559.2 Å		0.93 Å/pix.
x 600 pix.
= 559.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.932 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 1.0
Minimum - Maximum0.0 - 19.753530000000001
Average (Standard dev.)0.010907599 (±0.2723172)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions600600600
Spacing600600600
CellA=B=C: 559.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : dimeric WDR11-FAM91A1-C17orf75 complex

EntireName: dimeric WDR11-FAM91A1-C17orf75 complex
Components
  • Complex: dimeric WDR11-FAM91A1-C17orf75 complex
    • Protein or peptide: WD repeat-containing protein 11
    • Protein or peptide: Protein Njmu-R1
    • Protein or peptide: Protein FAM91A1

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Supramolecule #1: dimeric WDR11-FAM91A1-C17orf75 complex

SupramoleculeName: dimeric WDR11-FAM91A1-C17orf75 complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: WD repeat-containing protein 11

MacromoleculeName: WD repeat-containing protein 11 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 138.330922 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MASDYKDDDD KGSGMLPYTV NFKVSARTLT GALNAHNKAA VDWGWQGLIA YGCHSLVVVI DSITAQTLQV LEKHKADVVK VKWARENYH HNIGSPYCLR LASADVNGKI IVWDVAAGVA QCEIQEHAKP IQDVQWLWNQ DASRDLLLAI HPPNYIVLWN A DTGTKLWK ...String:
MASDYKDDDD KGSGMLPYTV NFKVSARTLT GALNAHNKAA VDWGWQGLIA YGCHSLVVVI DSITAQTLQV LEKHKADVVK VKWARENYH HNIGSPYCLR LASADVNGKI IVWDVAAGVA QCEIQEHAKP IQDVQWLWNQ DASRDLLLAI HPPNYIVLWN A DTGTKLWK KSYADNILSF SFDPFDPSHL TLLTSEGIVF ISDFSPSKPP SGPGKKVYIS SPHSSPAHNK LATATGAKKA LN KVKILIT QEKPSAEFIT LNDCLQLAYL PSKRNHMLLL YPREILILDL EVNQTVGVIA IERTGVPFLQ VIPCFQRDGL FCL HENGCI TLRVRRSYNN IFTTSNEEPD PDPVQELTYD LRSQCDAIRV TKTVRPFSMV CCPVNENAAA LVVSDGRVMI WELK SAVCN RNSRNSSSGV SPLYSPVSFC GIPVGVLQNK LPDLSLDNMI GQSAIAGEEH PRGSILREVH LKFLLTGLLS GLPAP QFAI RMCPPLTTKN IKMYQPLLAV GTSNGSVLVY HLTSGLLHKE LSIHSCEVKG IEWTSLTSFL SFATSTPNNM GLVRNE LQL VDLPTGRSIA FRGERGNDES AIEMIKVSHL KQYLAVVFRD KPLELWDVRT CTLLREMSKN FPTITALEWS PSHNLKS LR KKQLATREAM ARQTVVSDTE LSIVESSVIS LLQEAESKSE LSQNISAREH FVFTDIDGQV YHLTVEGNSV KDSARIPP D GSMGSITCIA WKGDTLVLGD MDGNLNFWDL KGRVSRGIPT HRSWVRKIRF APGKGNQKLI AMYNDGAEVW DTKEVQMVS SLRSGRNVTF RILDVDWCTS DKVILASDDG CIRVLEMSMK SACFRMDEQE LTEPVWCPYL LVPRASLALK AFLLHQPWNG QYSLDISHV DYPENEEIKN LLQEQLNSLS NDIKKLLLDP EFTLLQRCLL VSRLYGDESE LHFWTVAAHY LHSLSQEKSA S TTAPKEAA PRDKLSNPLD ICYDVLCENA YFQKFQLERV NLQEVKRSTY DHTRKCTDQL LLLGQTDRAV QLLLETSADN QH YYCDSLK ACLVTTVTSS GPSQSTIKLV ATNMIANGKL AEGVQLLCLI DKAADACRYL QTYGEWNRAA WLAKVRLNPE ECA DVLRRW VDHLCSPQVN QKSKALLVLL SLGCFFSVAE TLHSMRYFDR AALFVEACLK YGAFEVTEDT EKLITAIYAD YARS LKNLG FKQGAVLFAS KAGAAGKDLL NELESPKEEP IEE

UniProtKB: WD repeat-containing protein 11

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Macromolecule #2: Protein Njmu-R1

MacromoleculeName: Protein Njmu-R1 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 46.157922 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MASDYKDDDD KGSGMLPSLQ ESMDGDEKEL ESSEEGGSAE ERRLEPPSSS HYCLYSYRGS RLAQQRGDSE DGSPSGTNAE TPSGDDFSL SLADTNLPSE VEPELRSFIA KRLSRGAVFE GLGNVASVEL KIPGYRVGCY YCLFQNEKLL PETVTIDSER N PSEYVVCF ...String:
MASDYKDDDD KGSGMLPSLQ ESMDGDEKEL ESSEEGGSAE ERRLEPPSSS HYCLYSYRGS RLAQQRGDSE DGSPSGTNAE TPSGDDFSL SLADTNLPSE VEPELRSFIA KRLSRGAVFE GLGNVASVEL KIPGYRVGCY YCLFQNEKLL PETVTIDSER N PSEYVVCF LGGSEKGLEL FRLELDKYIQ GLKNNMNCEA RGLESHIKSY LSSWFEDVVC PIQRVVLLFQ EKLTFLLHAA LS YTPVEVK ESDEKTKRDI NRFLSVASLQ GLIHEGTMTS LCMAMTEEQH KSVVIDCSSS QPQFCNAGSN RFCEDWMQAF LNG AKGGNP FLFRQVLENF KLKAIQDTNN LKRFIRQAEM NHYALFKCYM FLKNCGSGDI LLKIVKVEHE EMPEAKNVIA VLEE FMKEA LDQSF

UniProtKB: Protein Njmu-R1

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Macromolecule #3: Protein FAM91A1

MacromoleculeName: Protein FAM91A1 / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 95.433375 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MGYPYDVPDY AGSMNIDVEF HIRHNYPWNK LPANVRQSLG NSQREYEKQV VLYSIRNQLR YRNNLVKHVK KDERRYYEEL LKYSRDHLM LYPYHLSDIM VKGLRITPFS YYTGIMEDIM NSEKSYDSLP NFTAADCLRL LGIGRNQYID LMNQCRSSKK F FRRKTARD ...String:
MGYPYDVPDY AGSMNIDVEF HIRHNYPWNK LPANVRQSLG NSQREYEKQV VLYSIRNQLR YRNNLVKHVK KDERRYYEEL LKYSRDHLM LYPYHLSDIM VKGLRITPFS YYTGIMEDIM NSEKSYDSLP NFTAADCLRL LGIGRNQYID LMNQCRSSKK F FRRKTARD LLPIKPVEIA IEAWWVVQAG YITEDDIKIC TLPEKCAVDK IIDSGPQLSG SLDYNVVHSL YNKGFIYLDV PI SDDSCIA VPPLEGFVMN RVQGDYFETL LYKIFVSIDE HTNVAELANV LEIDLSLVKN AVSMYCRLGF AHKKGQVINL DQL HSSWKN VPSVNRLKST LDPQKMLLSW DGGESRSPVQ EASSATDTDT NSQEDPADTA SVSSLSLSTG HTKRIAFLFD STLT AFLMM GNLSPNLKSH AVTMFEVGKL SDESLDSFLI ELEKVQSTGE GEAQRYFDHA LTLRNTILFL RHNKDLVAQT AQPDQ PNYG FPLDLLRCES LLGLDPATCS RVLNKNYTLL VSMAPLTNEI RPVSSCTPQH IGPAIPEVSS VWFKLYIYHV TGQGPP SLL LSKGTRLRKL PDIFQSYDRL LITSWGHDPG VVPTSNVLTM LNDALTHSAV LIQGHGLHGI GETVHVPFPF DETELQG EF TRVNMGVHKA LQILRNRVDL QHLCGYVTML NASSQLADRK LSDASDERGE PDLASGSDVN GSTESFEMVI EEATIDSA T KQTSGATTEA DWVPLELCFG IPLFSSELNR KVCRKIAAHG LCRKESLQNL LHSSRKLSLQ VLNFVHSFQE GASILDIHT EPSFSSLLSQ SSCADMGVPL PAKNLIFKDG VLSEWSGRSP SSLLIANLHL Q

UniProtKB: Protein FAM91A1

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.07 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 190979
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING

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