EMDB-65581: Cryo-EM structure of complex I on the bovine heart submitochondri...

Basic information

Entry

Database: EMDB / ID: EMD-65581

• Title: Cryo-EM structure of complex I on the bovine heart submitochondrial particles, closed

Sample

• Complex: Complex I on the bovine heart submitochondrial particles
  • Protein or peptide: x 42 types
• Protein or peptide: x 3 types
• Ligand: x 12 types

• Keywords: respiratory chain complex / supercomplex / submitochondrial particles / MOTOR PROTEIN / OXIDOREDUCTASE

Function / homology

Function:

Complex I biogenesis / Mitochondrial protein import / Protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / RHOG GTPase cycle / Complex I biogenesis / Mitochondrial ribosome-associated quality control / Complex I biogenesis / Respiratory electron transport / Respiratory electron transport / NADH dehydrogenase complex / Respiratory electron transport / protein lipoylation / cellular response to oxygen levels / iron-sulfur cluster assembly complex / ubiquinone biosynthetic process / mitochondrial large ribosomal subunit binding / mitochondrial [2Fe-2S] assembly complex / gliogenesis / Mitochondrial translation termination / Neutrophil degranulation / neural precursor cell proliferation / [2Fe-2S] cluster assembly / oxygen sensor activity / iron-sulfur cluster assembly / Mitochondrial protein degradation / NADH:ubiquinone reductase (H+-translocating) / ubiquinone binding / mitochondrial ATP synthesis coupled electron transport / electron transport coupled proton transport / proton motive force-driven mitochondrial ATP synthesis / mitochondrial electron transport, NADH to ubiquinone / acyl binding / mitochondrial respiratory chain complex I assembly / NADH dehydrogenase activity / oxidoreductase activity, acting on NAD(P)H / respiratory chain complex I / NADH dehydrogenase (ubiquinone) activity / response to cAMP / acyl carrier activity / quinone binding / ATP synthesis coupled electron transport / reactive oxygen species metabolic process / Mitochondrial protein degradation / neurogenesis / aerobic respiration / fatty acid binding / respiratory electron transport chain / electron transport chain / circadian rhythm / brain development / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / mitochondrial membrane / NAD binding / fatty acid biosynthetic process / FMN binding / 4 iron, 4 sulfur cluster binding / oxidoreductase activity / mitochondrial inner membrane / mitochondrial matrix / negative regulation of DNA-templated transcription / apoptotic process / protein-containing complex binding / structural molecule activity / mitochondrion / RNA binding / nucleoplasm / metal ion binding / cytoplasm

Domain/homology:

Complex1_LYR-like / NADH-ubiquinone oxidoreductase 1 subunit C1 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, NDUB1 / MNLL subunit / NADH-ubiquinone oxidoreductase flavoprotein 3 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / Tim17/Tim22/Tim23/Pmp24 family / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, metazoa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, animal type / NADH dehydrogenase 1 beta subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUC2 / NADH:ubiquinone oxidoreductase, subunit NDUFB4 / NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2) / NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4) / : / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH dehydrogenase 1, beta subcomplex, subunit 6 / NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit / NADH:ubiquinone oxidoreductase chain 4, N-terminal / NADH:ubiquinone oxidoreductase, chain 2 / NADH dehydrogenase subunit 2, C-terminal / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / : / : / NADH-ubiquinone oxidoreductase chain 4, amino terminus / NADH dehydrogenase subunit 2 C-terminus / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / NADH-ubiquinone oxidoreductase MWFE subunit / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / : / NADH dehydrogenase 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain / Zinc finger, CHCC-type / Zinc-finger domain / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / GRIM-19 / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NDUFB9, LYR domain / Deoxynucleoside kinase domain / Deoxynucleoside kinase / NDUFA6, LYR domain / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / : / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / : / NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain / : / SLBB domain / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH-quinone oxidoreductase, chain I / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH-ubiquinone oxidoreductase chain 4L/K / NADH-quinone oxidoreductase, chain M/4 / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like / NADH-ubiquinone/plastoquinone oxidoreductase chain 4L / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / NAD(P)H-quinone oxidoreductase subunit D/H / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / NADH ubiquinone oxidoreductase, F subunit / NADH-quinone oxidoreductase, subunit D

Component:

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 6 / NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial / NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 / Acyl carrier protein, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial / NADH dehydrogenase [ubiquinone] 1 subunit C2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 1 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH-ubiquinone oxidoreductase chain 3 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / Acyl carrier protein, mitochondrial

• Biological species: Bos taurus (domestic cattle)
• Method: single particle reconstruction / cryo EM / Resolution: 2.8 Å
• Authors: Nakano A / Masuya T / Akisada S / Ishikawa-Fukuda M / Mitsuoka K / Miyoshi H / Murai M / Yokoyama K

Funding support

Japan, 3 items

Organization	Grant number	Country
Japan Society for the Promotion of Science (JSPS)	23H02453	Japan
Japan Society for the Promotion of Science (JSPS)	25K01958	Japan
Ministry of Education, Culture, Sports, Science and Technology (Japan)		Japan

• Citation: Journal: Nat Commun / Year: 2026; Title: Structures of respiratory supercomplexes and ATP synthase oligomers in mammalian mitochondrial inner membrane.; Authors: Atsuki Nakano / Takahiro Masuya / Shinsuke Akisada / Moe Ishikawa-Fukuda / Kaoru Mitsuoka / Hideto Miyoshi / Masatoshi Murai / Ken Yokoyama / ; Abstract: Understanding the functional mechanisms of membrane protein complexes requires structural analysis within their native membrane environment. Here, we applied cryo-electron microscopy to determine the structures of FF ATP synthase and respiratory supercomplexes (SCs) on sub-mitochondrial particles (SMPs) isolated from bovine heart mitochondria. Most FF complexes were observed as dimers stabilized by the regulatory factor IF₁, and a tetrameric assembly comprising two FF-IF₁ dimers arranged linearly was also identified. This finding indicates that the tetrameric units of FF are present in the mitochondrial inner membrane and contribute to shaping cristae tips in mammalian mitochondria. F domain maps resolve the e-subunit- c₈-ring interface and show no discrete density for a tightly bound lipid within the c₈-ring. In addition to the previously reported SCs compositions CI₁CIII₂CIV₁ and CI₁CIII₂CIV₂, our analysis identified an additional assembly with the composition CI₁CIII₂CIV₃, as well as a CI₂CIII₂CIV₆ mega-complex. This approach enables rapid structural determination of FF ATP synthase and SCs from minimal membrane fractions, providing a foundation for elucidating the molecular basis of metabolic disorders and mitochondrial diseases at the level of higher-order architecture.

History

Deposition	Jul 28, 2025	-
Header (metadata) release	Apr 1, 2026	-
Map release	Apr 1, 2026	-
Update	Apr 1, 2026	-
Current status	Apr 1, 2026	Processing site: PDBj / Status: Released

Map

• File: Download / File: emd_65581.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.09 Å

Density

Contour Level	By AUTHOR: 0.29
Minimum - Maximum	-0.5844577 - 2.0014608
Average (Standard dev.)	-0.00019763396 (±0.02759837)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 600 600 600 Spacing 600 600 600
Cell	A=B=C: 654.0 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_65581_msk_1.map

Additional map: #1

• File: emd_65581_additional_1.map

Half map: #2

• File: emd_65581_half_map_1.map

Half map: #1

• File: emd_65581_half_map_2.map

Sample components

Entire : Complex I on the bovine heart submitochondrial particles

• Entire: Name: Complex I on the bovine heart submitochondrial particles

Components

• Complex: Complex I on the bovine heart submitochondrial particles
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 3
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
  • Protein or peptide: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 1
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 6
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4L
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 5
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 2
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
  • Protein or peptide: Acyl carrier protein, mitochondrial
  • Protein or peptide: Acyl carrier protein, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 subunit C2
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
  • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
• Ligand: N-FORMYLMETHIONINE
• Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine
• Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
• Ligand: IRON/SULFUR CLUSTER
• Ligand: FE2/S2 (INORGANIC) CLUSTER
• Ligand: FLAVIN MONONUCLEOTIDE
• Ligand: POTASSIUM ION
• Ligand: CARDIOLIPIN
• Ligand: MAGNESIUM ION
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
• Ligand: ZINC ION

Supramolecule #1: Complex I on the bovine heart submitochondrial particles

• Supramolecule: Name: Complex I on the bovine heart submitochondrial particles; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#24, #26-#34, #36-#43, #45
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 600 KDa

Macromolecule #1: NADH-ubiquinone oxidoreductase chain 3

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 3 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 12.927325 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

NLMLALLTNF TLATLLVIIA FWLPQLNVYS EKTSPYECGF DPMGSARLPF SMKFFLVAIT FLLFDLEIAL LLPLPWASQT ANLNTMLTM ALFLIILLAV SLAYEWTQKG LEWTE

UniProtKB: NADH-ubiquinone oxidoreductase chain 3

Macromolecule #2: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial; type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 17.773889 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

RGEYVVAKLD DLINWARRSS LWPMTFGLAC CAVEMMHMAA PRYDMDRFGV VFRASPRQSD VMIVAGTLTN KMAPALRKVY DQMPEPRYV VSMGSCANGG GYYHYSYSVV RGCDRIVPVD IYVPGCPPTA EALLYGILQL QKKIKREKRL RIWYRR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

Macromolecule #3: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial; type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 24.547701 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

DTRPTVRPRN DVAHKQLSAF GEYVAEILPK YVQQVQVSCF NELEICIHPD GVIPVLTFLR DHSNAQFKSL ADLTAVDIPT RQNRFEIVY NLLSLRFNSR IRVKTYTDEL TPIESSVPVY KAANWYEREI WDMFGVFFAN HPDLRRILTD YGFEGHPFRK D FPLSGYVE LRYDDEVKRV VAEPVELAQE FRKFDLNSPW EAFPAYRQPP E

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

Macromolecule #4: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial; type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 49.26341 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

ARQWQPDVEW AEQYGGAVMY PTKETAHWKP PPWNDVDPPK DTLVSNLTLN FGPQHPAAHG VLRLVMELSG EMVRKCDPHI GLLH(2MR)GTEK LIEYKTYLQA LPYFDRLDYV SMMCNEQAYS LAVEKLLNIR PPPRAQWIRV LFGEITRLLN HIMAVTT HA LDIGAMTPFF WMFEEREKMF EFYERVSGAR MHAAYVRPGG VHQDLPLGLM DDIYEFSKNF SLRIDELEEM LTNNRIWR N RTVDIGIVTA EDALNYGFSG VMLRGSGIQW DLRKTQPYDV YDQVEFDVPI GSRGDCYDRY LCRVEEMRQS IRIISQCLN KMPPGEIKVD DAKVSPPKRA EMKTSMESLI HHFKLYTEGY QVPPGATYTA IEAPKGEFGV YLVSDGSSRP YRCKIKAPGF AHLAGLDKM SKGHMLADVV AIIGTQDIVF GEVDR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

Macromolecule #5: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 23.655178 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

GALFVHRDTP ENNPETPFDF TPENYKRIEA IVKNYPEGHK AAAVLPVLDL AQRQNGWLPI SAMNKVAEIL QVPPMRVYEV ATFYTMYNR KPVGKYHIQV CTTTPCMLRN SDSILEAIQK KLGIKVGETT PDKLFTLIEV ECLGACVNAP MVQINDNYYE D LTPKDIEE IIDELKAGKI PKPGPRSGRF SCEPAGGLTS LTEPPKGPGF GVQAGL

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Macromolecule #6: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial; type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 47.383906 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

PKKTSFGSLK DEDRIFTNLY GRHDWRLKGA QSRGDWYKTK EILLKGPDWI LGEVKTSGLR GRGGAGFPTG LKWSFMNKPS DGRPKYLVV NADEGEPGTC KDREIIRHDP HKLVEGCLVG GRAMGARAAY IYIRGEFYNE ASNLQVAIRE AYEAGLIGKN A CGSGYDFD VFVVRGAGAY ICGEETALIE SIEGKQGKPR LKPPFPADVG VFGCPTTVAN VETVAVSPTI CRRGGAWFAS FG RERNSGT KLFNISGHVN NPCTVEEEMS VPLKELIEKH AGGVTGGWDN LLAVIPGGSS TPLIPKSVCE TVLMDFDALI QAQ TGLGTA AVIVMDRSTD IVKAIARLIE FYKHESCGQC TPCREGVDWM NKVMARFVRG DARPAEIDSL WEISKQIEGH TICA LGDGA AWPVQGLIRH FRPELEERMQ QFAQQHQ

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

Macromolecule #7: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

• Macromolecule: Name: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 76.628633 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

SNLIEVFVDG QSVMVEPGTT VLQACEKVGM QIPRFCYHER LSVAGNCRMC LVEIEKAPKV VAACAMPVMK GWNILTNSEK TKKAREGVM EFLLANHPLD CPICDQGGEC DLQDQSMMFG SDRSRFLEGK RAVEDKNIGP LVKTIMTRCI QCTRCIRFAS E IAGVDDLG TTGRGNDMQV GTYIEKMFMS ELSGNIIDIC PVGALTSKPY AFTARPWETR KTESIDVMDA VGSNIVVSTR TG EVMRILP RMHEDINEEW ISDKTRFAYD GLKRQRLTEP MVRNEKGLLT HTTWEDALSR VAGMLQSFQG NDVAAIAGGL VDA EALIAL KDLLNRVDSD TLCTEEVFPT AGAGTDLRSN YLLNTTIAGV EEADVVLLVG TNPRFEAPLF NARIRKSWLH NDLK VALIG SPVDLTYRYD HLGDSPKILQ DIASGSHPFS QVLQEAKKPM VILGSSALQR NDGAAILAAV SNIAQKIRTS SGVTG DWKV MNILHRIASQ VAALDLGYKP GVEAIQKNPP KMLFLLGADG GCITRQDLPK DCFIVYQGHH GDVGAPIADV ILPGAA YTE KSATYVNTEG RAQQTKVAVT PPGLAREDWK IIRALSEIAG MTLPYDTLDQ VRNRLEEVSP NLVRYDDVEG ANYFQQA SE LSKLVNQQLL ADPLVPPQLT IKDFYMTDSI SRASQTMAKC VKAVTEGAHA VEEPSIC

UniProtKB: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Macromolecule #8: NADH-ubiquinone oxidoreductase chain 1

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 35.716785 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

(FME)FMINILMLI IPILLAVAFL TLVERKVLGY MQLRKGPNVV GPYGLLQPIA DAIKLFIKEP LRPATSSASM FILAPI MAL GLALTMWIPL PMPYPLINMN LGVLFMLAMS SLAVYSILWS GWASNSKYAL IGALRAVAQT ISYEVTLAII LLSVLLM SG SFTLSTLITT QEQMWLILPA WPLAMMWFIS TLAETNRAPF DLTEGESELV SGFNVEYAAG PFALFFMAEY ANIIMMNI F TAILFLGTSH NPHMPELYTI NFTIKSLLLT MSFLWIRASY PRFRYDQLMH LLWKNFLPLT LALCMWHVSL PILTSGIPP QT

UniProtKB: NADH-ubiquinone oxidoreductase chain 1

Macromolecule #9: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial; type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 20.219947 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

TYKYVNLREP SMDMKSVTDR AAQTLLWTEL IRGLGMTLSY LFREPATINY PFEKGPLSPR FRGEHALRRY PSGEERCIAC KLCEAVCPA QAITIEAEPR ADGSRRTTRY DIDMTKCIYC GFCQEACPVD AIVEGPNFEF STETHEELLY NKEKLLNNGD K WEAEIAAN IQADYLYR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Macromolecule #10: NADH-ubiquinone oxidoreductase chain 6

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 6 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 19.110488 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

(FME)MLYIVFILS VIFVMGFVGF SSKPSPIYGG LGLIVSGGVG CGIVLNFGGS FLGLMVFLIY LGGMMVVFGY TTAMAT EQY PEIWLSNKAV LGAFVTGLLM EFFMVYYVLK DKEVEVVFEF NGLGDWVIYD TGDSGFFSEE AMGIAALYSY GTWLVIV TG WSLLIGVVVI MEITRGN

UniProtKB: NADH-ubiquinone oxidoreductase chain 6

Macromolecule #11: NADH-ubiquinone oxidoreductase chain 4L

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 4L / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 10.828195 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

(FME)SMVYMNIMM AFTVSLVGLL MYRSHLMSSL LCLEGMMLSL FVMAALTILN SHFTLASMMP IILLVFAACE AALGLS LLV MVSNTYGTDY VQNLNLLQC

UniProtKB: NADH-ubiquinone oxidoreductase chain 4L

Macromolecule #12: NADH-ubiquinone oxidoreductase chain 5

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 5 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 68.196727 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

NMFSSLSLVT LLLLTMPIMM MSFNTYKPSN YPLYVKTAIS YAFITSMIPT MMFIHSGQEL IISNWHWLTI QTLKLSLSFK MDYFSMMFI PVALFVTWSI MEFSMWYMYS DPNINKFFKY LLLFLITMLI LVTANNLFQL FIGWEGVGIM SFLLIGWWYG R ADANTAAL QAILYNRIGD IGFILAMAWF LTNLNTWDLQ QIFMLNPSDS NMPLIGLALA ATGKSAQFGL HPWLPSAMEG PT PVSALLH SSTMVVAGIF LLIRFYPLTE NNKYIQSITL CLGAITTLFT AMCALTQNDI KKIIAFSTSS QLGLMMVTIG INQ PYLAFL HICTHAFFKA MLFMCSGSII HSLNDEQDIR KMGGLFKAMP FTTTALIVGS LALTGMPFLT GFYSKDLIIE AANT SYTNA WALLMTLIAT SFTAIYSTRI IFFALLGQPR FPTLVNINEN NPLLINSIKR LLIGSLFAGY IISNNIPPTT IPQMT MPYY LKTTALIVTI LGFILALEIS NMTKNLKYHY PSNAFKFSTL LGYFPTIMHR LAPYMNLSMS QKSASSLLDL IWLEAI LPK TISLAQMKAS TLVTNQKGLI KLYFLSFLIT ILISMILFNF HE

UniProtKB: NADH-ubiquinone oxidoreductase chain 5

Macromolecule #13: NADH-ubiquinone oxidoreductase chain 4

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 4 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 51.999559 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

LKYIIPTIML MPLTWLSKNN MIWVNSTAHS LLISFTSLLL MNQFGDNSLN FSLLFFSDSL STPLLILTMW LLPLMLMASQ HHLSKENLT RKKLFITMLI SLQLFLIMTF TAMELILFYI LFEATLVPTL IIITRWGNQT ERLNAGLYFL FYTLAGSLPL L VALIYIQN TVGSLNFLML QYWVQPVHNS WSNVFMWLAC MMAFMVKMPL YGLHLWLPKA HVEAPIAGSM VLAAVLLKLG GY GMLRITL ILNPMTDFMA YPFIMLSLWG MIMTSSICLR QTDLKSLIAY SSVSHMALVI VAILIQTPWS YMGATALMIA HGL TSSMLF CLANSNYERI HSRTMILARG LQTLLPLMAT WWLLASLTNL ALPPTINLIG ELFVVMSTFS WSNITIILMG VNMV ITALY SLYMLIMTQR GKYTYHINNI SPSFTRENAL MSLHILPLLL LTLNPKIILG PLY

UniProtKB: NADH-ubiquinone oxidoreductase chain 4

Macromolecule #14: NADH-ubiquinone oxidoreductase chain 2

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 2 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 39.143734 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

NPIIFIIILL TIMLGTIIVM ISSHWLLVWI GFEMNMLAII PIMMKNHNPR ATEASTKYFL TQSTASMLLM MAVIINLMFS GQWTVMKLF NPMASMLMTM ALAMKLGMAP FHFWVPEVTQ GIPLSSGLIL LTWQKLAPMS VLYQIFPSIN LNLILTLSVL S ILIGGWGG LNQTQLRKIM AYSSIAHMGW MTAVLPYNPT MTLLNLIIYI IMTSTMFTMF MANSTTTTLS LSHTWNKTPI MT VLILATL LSMGGLPPLS GFMPKWMIIQ EMTKNNSIIL PTFMAITALL NLYFYMRLTY STTLTMFPST NNMKMKWQFP LMK KMTFLP TMVVLSTMML PLTPMLSVLE

UniProtKB: NADH-ubiquinone oxidoreductase chain 2

Macromolecule #15: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mi...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 36.754812 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

LQYGPLAYIL GEKATKKMTE KSKLITVDGN ICSGKSKLAK EIAEKLGLKH FPEAGIHYVD STTGDGKPLP VQFSGNCSLE KFYDDPKSN DGNSYRLQAW LYASRLLQYA DALEHLLSTG QGVVLERSIY SDFVFLEAMY RQGFIRKQCV DHYNQVKKVT I CEYLPPHV VVYVDVPVPE VQSRIQKKGN PHEMKITSAY LQDIENAYKG TFLPEMSEKC EVLQYSAWEA QDAEKVVEDI EY LKYDKGP WLDQNDRKLH KLRMLVQDKL EVLNYTSIPV FLPEVTVGAH QSDQVFQEFT ELPGRKYRAG YNEDVGDKWI WLK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial

Macromolecule #16: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mit...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial; type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 38.932789 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

LHHAVIPHGK GGRSSVSGIV ATVFGATGFL GRYVVNHLGR MGSQVIVPHR CEPYDTMHLR PMGDLGQIIF MDWNGRDKDS IRRAVEHSS VVINLVGREW ETQNFDFEDV FVKIPQAIAQ VSKEAGVEKF IHISHLNADI KSSSKYLRSK AVGEKEVRET F PEATIIKP AEIFGREDRF LNYFANIRWF GGVPLISLGK KTVKQPVYIV DVTKGIINAI KDPDARGKTF AFVGPSRYLL FD LVQYVFA VAHRPFLPYP LPHFAYRWIG RLFEISPFEP WTTRDKVERI HTTDKILPHL PGLEDLGVEA TPLELKAIEV LRR HRTYRW LSSEIEDVQP AKTIPT

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial

Macromolecule #17: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial; type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 14.918852 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

TRDTQLITVD EKLDITTITG VPEEHIKTRK ARIFVPARNN MQSGVNNTKK WKMEFDTRER WENPLMGWAS TADPLSNLVL TFSTKEDAV AFAEKNGWSY DVEERKVPKP KSKSYGANFS WNKRTRVSTK

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

Macromolecule #18: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial; type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 10.55172 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

GVRTSPTGEK VTHTGQVYDD GDYRKVRFVG RQKEVNENFA IDLIAEQPVS QVGSRVISCD GGGGALGHPR VYINLDKETK TGTCGYCGL QFRQQHH

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

Macromolecule #19: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 9.970458 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

KLGLREIRIH LCQRSPGSQG VRDFIEKRYV ELKKANPDLP ILIRECSDVQ PKLWARYAFG QEKNVSLNNF SADQVTRALE NVLSSKA

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

Macromolecule #20: Acyl carrier protein, mitochondrial

• Macromolecule: Name: Acyl carrier protein, mitochondrial / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 9.846298 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

PPLTLEGIKD RVLYVLKLYD KIDPEKLSVN SHFMKDLGLD SLDQVEIIMA MEDEFGFEIP DIDAEKLMCP QEIVDYIADK KDVYE

UniProtKB: Acyl carrier protein, mitochondrial

Macromolecule #21: Acyl carrier protein, mitochondrial

• Macromolecule: Name: Acyl carrier protein, mitochondrial / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 9.917375 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

APPLTLEGIK DRVLYVLKLY DKIDPEKLSV NSHFMKDLGL DSLDQVEIIM AMEDEFGFEI PDIDAEKLMC PQEIVDYIAD KKDVYE

UniProtKB: Acyl carrier protein, mitochondrial

Macromolecule #22: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5; type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 13.203414 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

AGLLKKTTGL VGLAVCETPH ERLKILYTKI LDVLGHIPKN AAYRKYTEQI TNEKLSMVKA EPDVKKLEER LQGGQIEEVI LQAENELSL ARKMIQWKPW EPLVEEPPAS QWKWPI

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

Macromolecule #23: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6; type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 13.88414 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

STSVKPIFSR DMNEAKRRVR ELYRAWYREV PNTVHLFQLD ISVKQGRDKV REMFKKNAHI TDPRVVDLLV IKGKMELEET IKVWKQRTH VMRFFHETEA PRPKDFLSKF YVGHDP

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

Macromolecule #24: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 19.992936 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

PGIVELPSLE DLKVQEVKVS SSVLKAAAHH YGAQCDKPNK EFMLCRWEEK DPRRCLEEGK LVNQCALEFF RQIKRHCAEP FTEYWTCID YSGLQLFRRC RKQQAQFDEC VLDKLGWVRP DLGDLSKVTK VKTDRPLPEN PYHSRARPEP NPEVEGDLKP A RHGSRLFF WTM

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

Macromolecule #25: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11; type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 14.666862 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

(ACE)AKTVLRQYW DIPEGTECHR KTYATTSIGG AAGLVVSAYS VALKTPTSFL EGVARTGRYT FTAAAIGAIF GLTSCI SAQ VREKPDDPLN YLIGGCAGGL ILGARTRSYG IGAAACAYMG LTAALVKMGQ LEGWQVFAEP KV

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

Macromolecule #26: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13; type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 16.421041 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

SKVKQDMPPV GGYGPIDYKR NLPRRGLSGY SMFAVGIGAL LFGYWSMMKW NRERRRLQIE DFEARIALMP LLQAEKDRRV LQMLRENLE EEATVMKDVP GWKVGESVFH TTRWVTPMMG ELYGLRASEE VLSATYGFIW YT

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

Macromolecule #27: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 8.117445 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

MWFEVLPGIA VMGVCLFIPG MATARIHRFS NGGKEKRVAH YPYQWYLMER DRRVSGVNRS YVSKGLENID

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

Macromolecule #28: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3; type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 9.226654 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

AERVAAFLKN VWAKEPVLVA SFAIAGLAVI LPTLSPYTKY SLMINRATPY NYPVPLRDDG NMPDVPSHPQ DPQGPSLEWL KRL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

Macromolecule #29: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial; type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 5.836698 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

KFYIQEPPHG SPNWLKVGLT LGTSVFLWIY LIKQHNEDVL EYKRRNGLE

UniProtKB: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

Macromolecule #30: NADH dehydrogenase [ubiquinone] 1 subunit C2

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 subunit C2 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 13.986099 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

MTGRQGRATF QFLPDEARSL PPPKLTDPRL AFVGFLGYCS GLIDNAIRRR PVLLAGLHRQ LLYITSFVFV GYYLLKRQDY MYAVRDHDM FSYIKSHPED FPEKDKKTYG EVFEEFHPVR

UniProtKB: NADH dehydrogenase [ubiquinone] 1 subunit C2

Macromolecule #31: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 11.835733 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

PFFDVQKRLG VDLDRWMTIQ SAEQPHKIPS RCHAFEKEWI ECAHGIGSIR AEKECKIEFE DFRECLLRQK TMKRLHAIRR QREKLIKEG KYTPPPHHSG

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

Macromolecule #32: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1; type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 6.978148 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

MNLLQVVRDH WVHVLVPMGF VFGYYLDRKN DEKLTAFRNK SLLYKRELKP NEEVTWK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

Macromolecule #33: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mit...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial; type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 11.897325 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

TLRWQEDPEP EDENLYEKNP DSHGYDKDPA VDIWNMRVVF FFGFSIVLVL GSTFVAYLPD YRMQEWARRE AERLVKYREA HGLPIMESN CFDPSKIQLP E

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial

Macromolecule #34: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mito...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial; type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 16.297941 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

KRLFIIKPSG FYDKRFLKLL RFYILLTGIP VAIGITLINV FIGEAELAEI PEGYVPEHWE YFKHPISRWI ARTFFDGPEK NYERTMAIL QIEAEKAELR LKELEVRRLM RARGDGPWFH YPTIDKELID HSPKATPDN

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial

Macromolecule #35: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6; type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 15.444929 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

(ACE)SGYTPEEKL RLQQLRELRR RWLKDQELSP REPVLPPQRV SPVERFWNKF LQDGALWKNV IYKTYRHSIF AFTHVL IPV WIIHYYLKYH VTTKPYTIVE KKPRIFPGDT ILETGEVIPP MKEFPDQHH

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

Macromolecule #36: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mito...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial; type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 8.385201 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

AGGGAHIEPR YRQFPQLTRS QVIQAEFFSA TMWFWILWRF WHDSDAVLGH FPYPDPSQWT DEELGIPPDD E

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial

Macromolecule #37: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3; type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 9.229537 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

SKMELPDYKQ WKIEGTPLET VQEKLAARGL RDPWGRNEAW RYMGGFANNV SFVGALLKGF KWGFAAFVVA IGAEYYLESQ K

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

Macromolecule #38: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 18.599799 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

HITKDMLPGP YPKTPEERAA AAKKYNMRVE DYEPYPDDGT GYGDYPKLPD RSQQERDPWY DWDHPDLRLN WGEPMHWDLD MYIRNRVDT SPTPVNWNLM CKHLFGFVAF MLFMFWVGET YPAYQPVGPK QYPYNNLYLE RGGDPNKEPE PVVHYEI

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

Macromolecule #39: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4; type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 15.075063 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

SFPKYEASRL SSLPTTLDPA EYDISSETRK AQAERLAIRS RLKREYQLQY YDPSRRGVIE DPALVRWTYA RSANIYPNFR PNTKTSLLG ALFGIGPLVF WYYVFKTDRD RKEKLIQEGK LDRTFNISY

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

Macromolecule #40: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 21.134076 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

AYLTHQQKVL RLYKRALRHL ESWCIHRDKY RYFACLLRAR FDEHKNEKDM VKATQLLREA EEEFWHGQHP QPYIFPESPG GTSYERYEC YKVPEWCLDD WHPSEKAMYP DYFAKREQWK KLRRESWERE VKQLQEETPV GGPRTEALPP ARKQGDLPPL W WHIVTRPR ERPM

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

Macromolecule #41: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7; type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 14.97812 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

GAHLARRYLG DASVEPDPLR MPTFPPDYGF PERKEREMVA TQQEMNDAQL VLQQRDYCAH YLIRFLKCKR DSFPNFLACK HERHDWDYC EHLDYVKRMK EFERERRLLQ RKKRREQREA DMA

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

Macromolecule #42: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10; type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 20.70151 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

DSWDKDVYPE PPRRTPAPSP QTSLPNPITY LTKAFDLLVD RPVTLVREFI ERQHAKNKYY YYHREFRRVP DITECQEKDV LCMFEAEMQ WRRDYKVDQE IVNIIQERLK ACQQREGESH RQNCAKELEQ FTQVVKAYQD RYHDLGAHYS ARKCLAKQKQ R MLAERKAA KEAAA

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

Macromolecule #43: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12; type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 17.115508 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

MELLQVLKRG LQQVSGHGGL RGYLRVLFRA NDVRVGTLVG EDKYGNKYYE DNKQFFGRHR WVIYTTEMNG KNTFWDVDGS MVPPEWHRW LHCMTDDPPT VKPPTARKFI WTNHKFNLSG TPQQYVPYST TRKKIQEWVP PSTPYK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

Macromolecule #44: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7; type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 12.590425 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

(ACE)ASATRFIQW LRNWASGRDL QAKLQLRYQE ISKRTQPPPK LPVGPSHKLS NNYYCTRDGR REAMPPSIVM SSQKVL VAG KPAESSAVAA SEKKAVSPAP PIKRWELSQD EPYL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

Macromolecule #45: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial; type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (domestic cattle)
• Molecular weight: Theoretical: 5.392883 KDa
• Recombinant expression: Organism: Bos taurus (domestic cattle)

Sequence

String:

EPFDNTTYKN LQHHDYSTYT FLDLNLDLSK FRMPQPSSGR ESPRH

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial

Macromolecule #46: N-FORMYLMETHIONINE

• Macromolecule: Name: N-FORMYLMETHIONINE / type: ligand / ID: 46 / Number of copies: 4 / Formula: FME
• Molecular weight: Theoretical: 177.221 Da

Chemical component information

ChemComp-FME:
N-FORMYLMETHIONINE

Macromolecule #47: 1,2-Distearoyl-sn-glycerophosphoethanolamine

• Macromolecule: Name: 1,2-Distearoyl-sn-glycerophosphoethanolamine / type: ligand / ID: 47 / Number of copies: 19 / Formula: 3PE
• Molecular weight: Theoretical: 748.065 Da

Chemical component information

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM

Macromolecule #48: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

• Macromolecule: Name: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 48 / Number of copies: 12 / Formula: PC1
• Molecular weight: Theoretical: 790.145 Da

Chemical component information

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM

Macromolecule #49: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 49 / Number of copies: 6 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

Macromolecule #50: FE2/S2 (INORGANIC) CLUSTER

• Macromolecule: Name: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 50 / Number of copies: 2 / Formula: FES
• Molecular weight: Theoretical: 175.82 Da

Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

Macromolecule #51: FLAVIN MONONUCLEOTIDE

• Macromolecule: Name: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 51 / Number of copies: 1 / Formula: FMN
• Molecular weight: Theoretical: 456.344 Da

Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

Macromolecule #52: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 52 / Number of copies: 1 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #53: CARDIOLIPIN

• Macromolecule: Name: CARDIOLIPIN / type: ligand / ID: 53 / Number of copies: 6 / Formula: CDL
• Molecular weight: Theoretical: 1.464043 KDa

Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

Macromolecule #54: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 54 / Number of copies: 1 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #55: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 55 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #56: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

• Macromolecule: Name: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE; type: ligand / ID: 56 / Number of copies: 1 / Formula: NDP
• Molecular weight: Theoretical: 745.421 Da

Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Macromolecule #57: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 57 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 298 K

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.8 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 30421492
• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Startup model: Type of model: INSILICO MODEL
• Final reconstruction: Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.7) / Number images used: 217828
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD

Atomic model buiding 1

Initial model

PDB ID:

6zpo

Chain - Source name: PDB / Chain - Initial model type: experimental model

• Refinement: Space: REAL / Protocol: FLEXIBLE FIT

Output model

PDB-9w2v:
Cryo-EM structure of complex I on the bovine heart submitochondrial particles, closed