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- EMDB-63175: Cryo-EM structure of the receptor of PL45-Olfr110-Gs complex -

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Basic information

Entry
Database: EMDB / ID: EMD-63175
TitleCryo-EM structure of the receptor of PL45-Olfr110-Gs complex
Map data
Sample
  • Complex: Cryo-EM structure of the receptor of PL45-Olfr110-Gs complex
    • Protein or peptide: Olfactory receptor
  • Ligand: methyl (9R,10E,12E)-9-methoxyoctadeca-10,12-dienoate
KeywordsOlfr110 / receptor / MEMBRANE PROTEIN
Function / homology
Function and homology information


olfactory receptor activity / detection of chemical stimulus involved in sensory perception of smell / G protein-coupled receptor activity / sensory perception of smell / G protein-coupled receptor signaling pathway / membrane / plasma membrane
Similarity search - Function
: / Olfactory receptor / Olfactory receptor / G-protein coupled receptors family 1 signature. / G protein-coupled receptor, rhodopsin-like / GPCR, rhodopsin-like, 7TM / G-protein coupled receptors family 1 profile.
Similarity search - Domain/homology
Biological speciesMus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsRong NK / Zhang MH / Yang F / Sun JP
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)81773704 China
CitationJournal: To Be Published
Title: Cryo-EM structure of the receptor of PL45-Olfr110-Gs complex
Authors: Rong NK / Zhang MH / Yang F / Sun JP
History
DepositionJan 16, 2025-
Header (metadata) releaseJan 21, 2026-
Map releaseJan 21, 2026-
UpdateJan 21, 2026-
Current statusJan 21, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_63175.png

Downloads & links

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Map

FileDownload / File: emd_63175.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.92 Å/pix.
x 256 pix.
= 235.52 Å		0.92 Å/pix.
x 256 pix.
= 235.52 Å		0.92 Å/pix.
x 256 pix.
= 235.52 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.92 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 4.56
Minimum - Maximum-0.12559775 - 24.513152999999999
Average (Standard dev.)-0.02662061 (±0.3094191)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 235.52 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_63175_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_63175_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_63175_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Cryo-EM structure of the receptor of PL45-Olfr110-Gs complex

EntireName: Cryo-EM structure of the receptor of PL45-Olfr110-Gs complex
Components
  • Complex: Cryo-EM structure of the receptor of PL45-Olfr110-Gs complex
    • Protein or peptide: Olfactory receptor
  • Ligand: methyl (9R,10E,12E)-9-methoxyoctadeca-10,12-dienoate

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Supramolecule #1: Cryo-EM structure of the receptor of PL45-Olfr110-Gs complex

SupramoleculeName: Cryo-EM structure of the receptor of PL45-Olfr110-Gs complex
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Mus musculus (house mouse)

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Macromolecule #1: Olfactory receptor

MacromoleculeName: Olfactory receptor / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 35.788172 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MEGKNQTAPS EFIILGFDHL NELQYLLFTI FFLTYICTLG GNVFIIVVTI ADSHLHTPMY YFLGNLALID ICYTTTNVPQ MMVHLLSEK KIISYGGCVT QLFAFIFFVG SECLLLAAMA YDRYIAICKP LRYSFIMNKA LCSWLAASCW TGGFLNSVLH T VLTFHLPF ...String:
MEGKNQTAPS EFIILGFDHL NELQYLLFTI FFLTYICTLG GNVFIIVVTI ADSHLHTPMY YFLGNLALID ICYTTTNVPQ MMVHLLSEK KIISYGGCVT QLFAFIFFVG SECLLLAAMA YDRYIAICKP LRYSFIMNKA LCSWLAASCW TGGFLNSVLH T VLTFHLPF CGNNQINYFF CDIPPLLILS CGDTSLNELA LLSIGILIGW TPFLCVILSY LYIISTILRI RSSEGRHKAF ST CASHLLI VILYYGSAIF TYVRPISSYS LEKDRLISVL YSVVTPMLNP VIYTLRNKDI KEAVKAIGRK WQPPVFSSDI

UniProtKB: Olfactory receptor

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Macromolecule #2: methyl (9R,10E,12E)-9-methoxyoctadeca-10,12-dienoate

MacromoleculeName: methyl (9R,10E,12E)-9-methoxyoctadeca-10,12-dienoate / type: ligand / ID: 2 / Number of copies: 1 / Formula: A1EKK
Molecular weightTheoretical: 324.498 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.2
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 1.875 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: DIFFRACTION / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.1) / Number images used: 168745
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION

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