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基本情報
登録情報 | ![]() | |||||||||
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タイトル | Human RNA Polymerase III de novo transcribing complex 4 (TC4) | |||||||||
![]() | Pol III TC4 main map | |||||||||
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![]() | initiation transcribing complex / TRANSCRIPTION/DNA/RNA / TRANSCRIPTION-DNA-RNA complex | |||||||||
機能・相同性 | ![]() snRNA-activating protein complex / snRNA transcription / transcription preinitiation complex assembly / snRNA transcription by RNA polymerase III / bent DNA binding / RNA polymerase III preinitiation complex assembly / TFIIIC-class transcription factor complex binding / RNA Polymerase III Chain Elongation / transcription factor TFIIIB complex / RNA Polymerase III Transcription Termination ...snRNA-activating protein complex / snRNA transcription / transcription preinitiation complex assembly / snRNA transcription by RNA polymerase III / bent DNA binding / RNA polymerase III preinitiation complex assembly / TFIIIC-class transcription factor complex binding / RNA Polymerase III Chain Elongation / transcription factor TFIIIB complex / RNA Polymerase III Transcription Termination / calcitonin gene-related peptide receptor activity / DNA/RNA hybrid binding / RNA polymerase III type 3 promoter sequence-specific DNA binding / RNA polymerase transcription factor SL1 complex / snRNA transcription by RNA polymerase II / regulation of transcription by RNA polymerase I / regulation of transcription by RNA polymerase III / RNA polymerase III general transcription initiation factor activity / RNA polymerase I core promoter sequence-specific DNA binding / RPAP3/R2TP/prefoldin-like complex / DNA polymerase III complex / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / transcription factor TFIIA complex / Cytosolic sensors of pathogen-associated DNA / RNA Polymerase III Abortive And Retractive Initiation / female germ cell nucleus / male pronucleus / female pronucleus / positive regulation of innate immune response / RNA polymerase II general transcription initiation factor binding / nucleobase-containing compound metabolic process / Abortive elongation of HIV-1 transcript in the absence of Tat / RNA Polymerase I Transcription Termination / FGFR2 alternative splicing / transcription preinitiation complex / MicroRNA (miRNA) biogenesis / Viral Messenger RNA Synthesis / Signaling by FGFR2 IIIa TM / RNA polymerase II general transcription initiation factor activity / transcription factor TFIID complex / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / HIV Transcription Initiation / RNA Polymerase II HIV Promoter Escape / Transcription of the HIV genome / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / mRNA Capping / transcription initiation at RNA polymerase III promoter / mRNA Splicing - Minor Pathway / PIWI-interacting RNA (piRNA) biogenesis / aryl hydrocarbon receptor binding / TFIIB-class transcription factor binding / RNA Polymerase I Transcription Initiation / Processing of Capped Intron-Containing Pre-mRNA / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / RNA polymerase II transcribes snRNA genes / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / neuropeptide signaling pathway / positive regulation of transcription initiation by RNA polymerase II / : / transcription by RNA polymerase III / Tat-mediated elongation of the HIV-1 transcript / Formation of HIV-1 elongation complex containing HIV-1 Tat / transcription by RNA polymerase I / RNA polymerase I complex / transcription elongation by RNA polymerase I / RNA polymerase III complex / Formation of HIV elongation complex in the absence of HIV Tat / RNA polymerase II core promoter sequence-specific DNA binding / core promoter sequence-specific DNA binding / RNA polymerase II, core complex / RNA polymerase II preinitiation complex assembly / tRNA transcription by RNA polymerase III / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex / RNA Polymerase II Pre-transcription Events / Inhibition of DNA recombination at telomere / mRNA Splicing - Major Pathway / positive regulation of interferon-beta production / TBP-class protein binding / acrosomal vesicle / male germ cell nucleus / SIRT1 negatively regulates rRNA expression / transcription initiation at RNA polymerase II promoter / TP53 Regulates Transcription of DNA Repair Genes / RNA Polymerase I Promoter Escape / DNA-templated transcription initiation / Transcriptional regulation by small RNAs / mRNA transcription by RNA polymerase II / DNA-directed RNA polymerase activity / euchromatin 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.5 Å | |||||||||
![]() | Wang Q / Ren Y / Jin Q / Chen X / Xu Y | |||||||||
資金援助 | 1件
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![]() | ![]() タイトル: Structural insights into human Pol III transcription initiation in action. 著者: Qianmin Wang / Yulei Ren / Qianwei Jin / Xizi Chen / Yanhui Xu / ![]() 要旨: RNA polymerase III (Pol III) transcribes highly demanded RNAs grouped into three types of classical promoters, including type 1 (5S rRNA), type 2 (tRNA) and type 3 (short non-coding RNAs, such as U6, ...RNA polymerase III (Pol III) transcribes highly demanded RNAs grouped into three types of classical promoters, including type 1 (5S rRNA), type 2 (tRNA) and type 3 (short non-coding RNAs, such as U6, 7SK and RNase H1) promoters. While structures of the Pol III preinitiation complex (PIC) and elongation complex (EC) have been determined, the mechanism underlying the transition from initiation to elongation remains unclear. Here we reconstituted seven human Pol III transcribing complexes (TC4, TC5, TC6, TC8, TC10, TC12 and TC13) halted on U6 promoters with nascent RNAs of 4-13 nucleotides. Cryo-electron microscopy structures captured initially transcribing complexes (ITCs; TC4 and TC5) and ECs (TC6-13). Together with KMnO footprinting, the data reveal extensive modular rearrangements: the transcription bubble expands from PIC to TC5, followed by general transcription factor (GTF) dissociation and abrupt bubble collapse from TC5 to TC6, marking the ITC-EC transition. In TC5, SNAPc and TFIIIB remain bound to the promoter and Pol III, while the RNA-DNA hybrid adopts a tilted conformation with template DNA blocked by BRF2, a TFIIIB subunit. Hybrid forward translocation during ITC-EC transition triggers BRF2-finger retraction, GTF release and transcription-bubble collapse. Pol III then escapes the promoter while GTFs stay bound upstream, potentially enabling reinitiation. These findings reveal molecular insights into Pol III dynamics and reinitiation mechanisms on type 3 promoters of highly demanded small RNAs, with the earliest documented initiation-elongation transition for an RNA polymerase. | |||||||||
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 4.2 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 60.3 KB 60.3 KB | 表示 表示 | ![]() |
画像 | ![]() | 110.5 KB | ||
Filedesc metadata | ![]() | 15.5 KB | ||
その他 | ![]() ![]() ![]() ![]() | 118.1 MB 115.2 MB 118.4 MB 118.4 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 811.1 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 810.6 KB | 表示 | |
XML形式データ | ![]() | 14.1 KB | 表示 | |
CIF形式データ | ![]() | 16.9 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 9k3vMC ![]() 9k2gC ![]() 9k36C ![]() 9k38C ![]() 9k39C ![]() 9k3bC ![]() 9k3uC ![]() 9lktC ![]() 9lxnC ![]() 9lxoC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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注釈 | Pol III TC4 main map | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.33254 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-追加マップ: SNAPC-TFIIIB-U6 local map
ファイル | emd_62030_additional_1.map | ||||||||||||
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注釈 | SNAPC-TFIIIB-U6 local map | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: Pol III TC4 postprocess map
ファイル | emd_62030_additional_2.map | ||||||||||||
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注釈 | Pol III TC4 postprocess map | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Pol III TC4 half map1
ファイル | emd_62030_half_map_1.map | ||||||||||||
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注釈 | Pol III TC4 half map1 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Pol III TC4 half map2
ファイル | emd_62030_half_map_2.map | ||||||||||||
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注釈 | Pol III TC4 half map2 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : RNA polymerase III transcribing complex 4
+超分子 #1: RNA polymerase III transcribing complex 4
+分子 #1: snRNA-activating protein complex subunit 1
+分子 #2: snRNA-activating protein complex subunit 3
+分子 #3: snRNA-activating protein complex subunit 4
+分子 #4: DNA-directed RNA polymerase III subunit RPC1
+分子 #5: DNA-directed RNA polymerase III subunit RPC2
+分子 #6: DNA-directed RNA polymerases I and III subunit RPAC1
+分子 #7: DNA-directed RNA polymerase III subunit RPC9
+分子 #8: DNA-directed RNA polymerases I, II, and III subunit RPABC1
+分子 #9: DNA-directed RNA polymerases I, II, and III subunit RPABC2
+分子 #10: DNA-directed RNA polymerase III subunit RPC8
+分子 #11: DNA-directed RNA polymerases I, II, and III subunit RPABC3
+分子 #12: DNA-directed RNA polymerase III subunit RPC10
+分子 #13: DNA-directed RNA polymerases I, II, and III subunit RPABC5
+分子 #14: DNA-directed RNA polymerases I and III subunit RPAC2
+分子 #15: DNA-directed RNA polymerases I, II, and III subunit RPABC4
+分子 #16: DNA-directed RNA polymerase III subunit RPC5
+分子 #17: DNA-directed RNA polymerase III subunit RPC4
+分子 #18: DNA-directed RNA polymerase III subunit RPC3
+分子 #19: DNA-directed RNA polymerase III subunit RPC6
+分子 #20: DNA-directed RNA polymerase III subunit RPC7
+分子 #21: TATA-box-binding protein
+分子 #22: Transcription factor IIIB 50 kDa subunit
+分子 #23: Transcription factor TFIIIB component B'' homolog
+分子 #24: DNA (96-MER)
+分子 #25: DNA (96-MER)
+分子 #26: RNA (5'-R(P*UP*GP*C)-3')
+分子 #27: ZINC ION
+分子 #28: 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE
+分子 #29: IRON/SULFUR CLUSTER
+分子 #30: MAGNESIUM ION
+分子 #31: GUANOSINE-5'-TRIPHOSPHATE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 8 |
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凍結 | 凍結剤: ETHANE-PROPANE |
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電子顕微鏡法
顕微鏡 | TFS KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 50.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: DARK FIELD / 最大 デフォーカス(公称値): 2.5 µm / 最小 デフォーカス(公称値): 1.5 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |