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- EMDB-60794: Cryo-EM structure of MT3-Muscarinic acetylcholine receptor 4 -

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Basic information

Entry
Database: EMDB / ID: EMD-60794
TitleCryo-EM structure of MT3-Muscarinic acetylcholine receptor 4
Map data
Sample
  • Complex: MT3-alpha2AAR complex
    • Protein or peptide: Muscarinic toxin 3
    • Protein or peptide: Muscarinic acetylcholine receptor M4,Soluble cytochrome b562
Keywordscomplex / MEMBRANE PROTEIN
Function / homology
Function and homology information


Muscarinic acetylcholine receptors / G protein-coupled acetylcholine receptor activity / adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway / regulation of locomotion / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / G protein-coupled acetylcholine receptor signaling pathway / toxin activity / G alpha (i) signalling events / chemical synaptic transmission / postsynaptic membrane ...Muscarinic acetylcholine receptors / G protein-coupled acetylcholine receptor activity / adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway / regulation of locomotion / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / G protein-coupled acetylcholine receptor signaling pathway / toxin activity / G alpha (i) signalling events / chemical synaptic transmission / postsynaptic membrane / cell surface receptor signaling pathway / synapse / dendrite / signal transduction / extracellular region / plasma membrane
Similarity search - Function
Muscarinic acetylcholine receptor M4 / Snake cytotoxin, cobra-type / Muscarinic acetylcholine receptor family / Snake toxin, conserved site / Snake toxins signature. / : / Snake toxin cobra-type / Snake three-finger toxin / Snake toxin-like superfamily / Serpentine type 7TM GPCR chemoreceptor Srsx ...Muscarinic acetylcholine receptor M4 / Snake cytotoxin, cobra-type / Muscarinic acetylcholine receptor family / Snake toxin, conserved site / Snake toxins signature. / : / Snake toxin cobra-type / Snake three-finger toxin / Snake toxin-like superfamily / Serpentine type 7TM GPCR chemoreceptor Srsx / G-protein coupled receptors family 1 signature. / G protein-coupled receptor, rhodopsin-like / GPCR, rhodopsin-like, 7TM / G-protein coupled receptors family 1 profile. / 7 transmembrane receptor (rhodopsin family)
Similarity search - Domain/homology
Muscarinic acetylcholine receptor M4 / Muscarinic toxin 3
Similarity search - Component
Biological speciesHomo sapiens (human) / Dendroaspis angusticeps (eastern green mamba)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsZhong YX / Tao HH / Tao YY
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: To Be Published
Title: Cryo-EM structure of MT3-alpha2AAR
Authors: Zhong YX / Tao HH / Tao YY
History
DepositionJul 13, 2024-
Header (metadata) releaseJul 23, 2025-
Map releaseJul 23, 2025-
UpdateJul 23, 2025-
Current statusJul 23, 2025Processing site: PDBj / Status: Released

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Structure visualization

Downloads & links

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Map

FileDownload / File: emd_60794.map.gz / Format: CCP4 / Size: 83.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.07 Å
Density
Contour LevelBy AUTHOR: 0.546
Minimum - Maximum-1.7069795 - 2.8597615
Average (Standard dev.)0.0005547446 (±0.046579957)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions280280280
Spacing280280280
CellA=B=C: 299.6 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : MT3-alpha2AAR complex

EntireName: MT3-alpha2AAR complex
Components
  • Complex: MT3-alpha2AAR complex
    • Protein or peptide: Muscarinic toxin 3
    • Protein or peptide: Muscarinic acetylcholine receptor M4,Soluble cytochrome b562

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Supramolecule #1: MT3-alpha2AAR complex

SupramoleculeName: MT3-alpha2AAR complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Muscarinic toxin 3

MacromoleculeName: Muscarinic toxin 3 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Dendroaspis angusticeps (eastern green mamba)
Molecular weightTheoretical: 7.396488 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
LTCVTKNTIF GITTENCPAG QNLCFKRWHY VIPRYTEITR GCAATCPIPE NYDSIHCCKT DKCNE

UniProtKB: Muscarinic toxin 3

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Macromolecule #2: Muscarinic acetylcholine receptor M4,Soluble cytochrome b562

MacromoleculeName: Muscarinic acetylcholine receptor M4,Soluble cytochrome b562
type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 48.768914 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: HNRYETVEMV FIATVTGSLS LVTVVGNILV MLSIKVNRQL QTVNNYFLFS LACADLIIGA FSMNLYTVYI IKGYWPLGAV VCDLWLALD YVVSNASVMN LLIISFDRYF CVTKPLTYPA RRTTKMAGLM IAAAWVLSFV LWAPAILFWQ FVVGKRTVPD N QCFIQFLS ...String:
HNRYETVEMV FIATVTGSLS LVTVVGNILV MLSIKVNRQL QTVNNYFLFS LACADLIIGA FSMNLYTVYI IKGYWPLGAV VCDLWLALD YVVSNASVMN LLIISFDRYF CVTKPLTYPA RRTTKMAGLM IAAAWVLSFV LWAPAILFWQ FVVGKRTVPD N QCFIQFLS NPAVTFGTAI AAFYLPVVIM TVLYIHISLA SRSRVADLED NWETLNDNLK VIEKADNAAQ VKDALTKMRA AA LDAQKAS GSGSPEMKDF RHGFDILVGQ IDDALKLANE GKVKEAQAAA EQLKTTRNAY IQKYLRMAAR ERKVTRTIFA ILL AFILTW TPYNVMVLVN TFCQSCIPDT VWSIGYWLCY VNSTINPACY ALCNATFKKT FRHLLLKIAA LKEKIAALKE KIAA LKEAE EKRASRLEEE LRRRLTEGSH HHHHHHH

UniProtKB: Muscarinic acetylcholine receptor M4, Muscarinic acetylcholine receptor M4

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statecell

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: NITROGEN / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS GLACIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 52.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 5.0 µm / Nominal defocus min: 1.2 µm

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 5747771
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION

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