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- PDB-9iqs: Cryo-EM structure of MT3-Muscarinic acetylcholine receptor 4 -

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Basic information

Entry
Database: PDB / ID: 9iqs
TitleCryo-EM structure of MT3-Muscarinic acetylcholine receptor 4
Components
  • Muscarinic acetylcholine receptor M4,Soluble cytochrome b562
  • Muscarinic toxin 3
KeywordsMEMBRANE PROTEIN / complex
Function / homology
Function and homology information


Muscarinic acetylcholine receptors / G protein-coupled acetylcholine receptor activity / adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway / regulation of locomotion / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / G protein-coupled acetylcholine receptor signaling pathway / toxin activity / G alpha (i) signalling events / chemical synaptic transmission / postsynaptic membrane ...Muscarinic acetylcholine receptors / G protein-coupled acetylcholine receptor activity / adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway / regulation of locomotion / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / G protein-coupled acetylcholine receptor signaling pathway / toxin activity / G alpha (i) signalling events / chemical synaptic transmission / postsynaptic membrane / cell surface receptor signaling pathway / synapse / dendrite / signal transduction / extracellular region / plasma membrane
Similarity search - Function
Muscarinic acetylcholine receptor M4 / Snake cytotoxin, cobra-type / Muscarinic acetylcholine receptor family / Snake toxin, conserved site / Snake toxins signature. / : / Snake toxin cobra-type / Snake three-finger toxin / Snake toxin-like superfamily / Serpentine type 7TM GPCR chemoreceptor Srsx ...Muscarinic acetylcholine receptor M4 / Snake cytotoxin, cobra-type / Muscarinic acetylcholine receptor family / Snake toxin, conserved site / Snake toxins signature. / : / Snake toxin cobra-type / Snake three-finger toxin / Snake toxin-like superfamily / Serpentine type 7TM GPCR chemoreceptor Srsx / G-protein coupled receptors family 1 signature. / G protein-coupled receptor, rhodopsin-like / GPCR, rhodopsin-like, 7TM / G-protein coupled receptors family 1 profile. / 7 transmembrane receptor (rhodopsin family)
Similarity search - Domain/homology
Muscarinic acetylcholine receptor M4 / Muscarinic toxin 3
Similarity search - Component
Biological speciesDendroaspis angusticeps (eastern green mamba)
Homo sapiens (human)
synthetic construct (others)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsZhong, Y.X. / Tao, H.H. / Tao, Y.Y.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: To Be Published
Title: Cryo-EM structure of MT3-alpha2AAR
Authors: Zhong, Y.X. / Tao, H.H. / Tao, Y.Y.
History
DepositionJul 13, 2024Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jul 23, 2025Provider: repository / Type: Initial release
Revision 1.0Jul 23, 2025Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0Jul 23, 2025Data content type: FSC / Data content type: FSC / Provider: repository / Type: Initial release
Revision 1.0Jul 23, 2025Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Jul 23, 2025Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Jul 23, 2025Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0Jul 23, 2025Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Muscarinic toxin 3
B: Muscarinic acetylcholine receptor M4,Soluble cytochrome b562


Theoretical massNumber of molelcules
Total (without water)56,1652
Polymers56,1652
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein Muscarinic toxin 3 / MT3


Mass: 7396.488 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Dendroaspis angusticeps (eastern green mamba)
Production host: Homo sapiens (human) / References: UniProt: P81031
#2: Protein Muscarinic acetylcholine receptor M4,Soluble cytochrome b562


Mass: 48768.914 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human), (gene. exp.) synthetic construct (others)
Gene: CHRM4 / Production host: Homo sapiens (human) / References: UniProt: P08173
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: CELL / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: MT3-alpha2AAR complex / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Source (natural)
IDEntity assembly-IDOrganismNcbi tax-ID
11Homo sapiens (human)9606
21Dendroaspis angusticeps (eastern green mamba)8618
Source (recombinant)Organism: Homo sapiens (human)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: NITROGEN

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Electron microscopy imaging

MicroscopyModel: TFS GLACIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 5000 nm / Nominal defocus min: 1200 nm
Image recordingElectron dose: 52 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 5747771 / Symmetry type: POINT

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