+
データを開く
-
基本情報
| 登録情報 | ![]() | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| タイトル | Influenza A/H7N9 polymerase pre-cleavage cap-snatching complex | |||||||||
マップデータ | H7N9 cap-snatching pre-cleavage complex consensus | |||||||||
試料 |
| |||||||||
キーワード | Influenza virus / influenza A / cap-snatching / transcription / VIRUS | |||||||||
| 機能・相同性 | 機能・相同性情報negative regulation of DNA-templated transcription, elongation / DSIF complex / regulation of transcription elongation by RNA polymerase II / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / RNA polymerase II transcribes snRNA genes ...negative regulation of DNA-templated transcription, elongation / DSIF complex / regulation of transcription elongation by RNA polymerase II / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / RNA polymerase II transcribes snRNA genes / mRNA Capping / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Elongation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Pol II CTD phosphorylation and interaction with CE / Estrogen-dependent gene expression / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / mRNA Splicing - Major Pathway / positive regulation of DNA-templated transcription, elongation / Abortive elongation of HIV-1 transcript in the absence of Tat / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE / transcription elongation-coupled chromatin remodeling / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / cap snatching / mRNA Capping / viral transcription / RNA polymerase II complex binding / symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / negative regulation of transcription elongation by RNA polymerase II / positive regulation of macroautophagy / RNA polymerase II transcribes snRNA genes / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / 7-methylguanosine mRNA capping / host cell mitochondrion / Tat-mediated elongation of the HIV-1 transcript / Formation of HIV-1 elongation complex containing HIV-1 Tat / RNA polymerase I complex / transcription elongation by RNA polymerase I / RNA polymerase III complex / core promoter sequence-specific DNA binding / Formation of HIV elongation complex in the absence of HIV Tat / RNA polymerase II, core complex / tRNA transcription by RNA polymerase III / transcription by RNA polymerase I / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex / translation initiation factor binding / transcription-coupled nucleotide-excision repair / RNA Polymerase II Pre-transcription Events / DNA-directed RNA polymerase complex / TP53 Regulates Transcription of DNA Repair Genes / transcription initiation at RNA polymerase II promoter / transcription elongation by RNA polymerase II / DNA-templated transcription initiation / positive regulation of transcription elongation by RNA polymerase II / euchromatin / virion component / ribonucleoside binding / fibrillar center / DNA-directed RNA polymerase / DNA-directed RNA polymerase activity / endonuclease activity / nucleic acid binding / 加水分解酵素; エステル加水分解酵素 / transcription by RNA polymerase II / host cell cytoplasm / protein dimerization activity / nuclear speck / symbiont-mediated suppression of host gene expression / protein heterodimerization activity / viral translational frameshifting / RNA-directed RNA polymerase / viral RNA genome replication / nucleotide binding / RNA-directed RNA polymerase activity / mRNA binding / DNA-templated transcription / chromatin binding / regulation of DNA-templated transcription / nucleolus / host cell nucleus / enzyme binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / mitochondrion / DNA binding / RNA binding / zinc ion binding 類似検索 - 分子機能 | |||||||||
| 生物種 | Homo sapiens (ヒト) / Influenza B virus (B型インフルエンザウイルス) / Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)) (A型インフルエンザウイルス) / synthetic construct (人工物) / ![]() | |||||||||
| 手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.3 Å | |||||||||
データ登録者 | Rotsch AH / Li D / Dienemann C / Cusack S / Cramer P | |||||||||
| 資金援助 | ドイツ, European Union, 2件
| |||||||||
引用 | ジャーナル: To Be Publishedタイトル: Mechanism of Co-Transcriptional Cap-Snatching by Influenza Polymerase 著者: Rotsch AH / Li D / Dupont M / Krischuns T / Oberthuer C / Stelfox A / Lukarska M / Fianu I / Lidschreiber M / Naffakh N / Dienemann C / Cusack S / Cramer P | |||||||||
| 履歴 |
|
-
構造の表示
| 添付画像 |
|---|
-
ダウンロードとリンク
-EMDBアーカイブ
| マップデータ | emd_50892.map.gz | 271.3 MB | EMDBマップデータ形式 | |
|---|---|---|---|---|
| ヘッダ (付随情報) | emd-50892-v30.xml emd-50892.xml | 71.1 KB 71.1 KB | 表示 表示 | EMDBヘッダ |
| 画像 | emd_50892.png | 69.6 KB | ||
| マスクデータ | emd_50892_msk_1.map emd_50892_msk_2.map emd_50892_msk_3.map | 343 MB 343 MB 343 MB | マスクマップ | |
| Filedesc metadata | emd-50892.cif.gz | 13.9 KB | ||
| その他 | emd_50892_additional_1.map.gz emd_50892_additional_2.map.gz emd_50892_additional_3.map.gz emd_50892_additional_4.map.gz emd_50892_additional_5.map.gz emd_50892_additional_6.map.gz emd_50892_additional_7.map.gz emd_50892_additional_8.map.gz emd_50892_additional_9.map.gz emd_50892_half_map_1.map.gz emd_50892_half_map_2.map.gz | 273.8 MB 318.9 MB 315.6 MB 275.4 MB 275.3 MB 316.4 MB 273.4 MB 274.9 MB 274.8 MB 274.4 MB 274.6 MB | ||
| アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-50892 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-50892 | HTTPS FTP |
-検証レポート
| 文書・要旨 | emd_50892_validation.pdf.gz | 987.9 KB | 表示 | EMDB検証レポート |
|---|---|---|---|---|
| 文書・詳細版 | emd_50892_full_validation.pdf.gz | 987.5 KB | 表示 | |
| XML形式データ | emd_50892_validation.xml.gz | 17.1 KB | 表示 | |
| CIF形式データ | emd_50892_validation.cif.gz | 20.5 KB | 表示 | |
| アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-50892 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-50892 | HTTPS FTP |
-関連構造データ
| 関連構造データ | ![]() 9fyxMC ![]() 9g0aC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
|---|---|
| 類似構造データ | 類似検索 - 機能・相同性 F&H 検索 |
-
リンク
| EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
|---|---|
| 「今月の分子」の関連する項目 |
-
マップ
| ファイル | ダウンロード / ファイル: emd_50892.map.gz / 形式: CCP4 / 大きさ: 343 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 注釈 | H7N9 cap-snatching pre-cleavage complex consensus | ||||||||||||||||||||||||||||||||||||
| 投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
| ボクセルのサイズ | X=Y=Z: 1.05 Å | ||||||||||||||||||||||||||||||||||||
| 密度 |
| ||||||||||||||||||||||||||||||||||||
| 対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
| 詳細 | EMDB XML:
|
-添付データ
+マスク #1
+マスク #2
+マスク #3
+追加マップ: H7N9 cap-snatching pre-cleavage complex Pol II focussed refinement
+追加マップ: H7N9 cap-snatching pre-cleavage complex consensus, post processed
+追加マップ: H7N9 cap-snatching pre-cleavage complex Pol II focussed refinement,...
+追加マップ: H7N9 cap-snatching pre-cleavage complex Pol II focussed refinement,...
+追加マップ: H7N9 cap-snatching pre-cleavage complex Pol II focussed refinement,...
+追加マップ: H7N9 cap-snatching pre-cleavage complex FluPol focussed refinement, post...
+追加マップ: H7N9 cap-snatching pre-cleavage complex FluPol focussed refinement
+追加マップ: H7N9 cap-snatching pre-cleavage complex FluPol focussed refinement, Halfmap...
+追加マップ: H7N9 cap-snatching pre-cleavage complex FluPol focussed refinement, Halfmap...
+ハーフマップ: H7N9 cap-snatching pre-cleavage complex consensus, half map 1
+ハーフマップ: H7N9 cap-snatching pre-cleavage complex consensus, half map 2
-
試料の構成要素
+全体 : Influenza A/H7N9 polymerase pre-cleavage cap-snatching complex
+超分子 #1: Influenza A/H7N9 polymerase pre-cleavage cap-snatching complex
+超分子 #2: Transcription elongation factors SPT4 and SPT5
+超分子 #3: RNA
+超分子 #4: Influenza virus RNA-dependent RNA polymerase
+超分子 #5: DNA
+超分子 #6: Human RNA polymerase II
+分子 #1: RNA polymerase II subunit D
+分子 #2: DNA-directed RNA polymerase II subunit E
+分子 #3: DNA-directed RNA polymerases I, II, and III subunit RPABC2
+分子 #4: DNA-directed RNA polymerase subunit
+分子 #5: DNA-directed RNA polymerases I, II, and III subunit RPABC3
+分子 #6: DNA-directed RNA polymerase II subunit RPB9
+分子 #7: DNA-directed RNA polymerases I, II, and III subunit RPABC5
+分子 #8: DNA-directed RNA polymerase II subunit RPB11-a
+分子 #9: RNA polymerase II, I and III subunit K
+分子 #13: Transcription elongation factor SPT4
+分子 #14: Transcription elongation factor SPT5
+分子 #15: DNA-directed RNA polymerase subunit
+分子 #16: DNA-directed RNA polymerase subunit beta
+分子 #17: DNA-directed RNA polymerase II subunit RPB3
+分子 #18: Polymerase acidic protein
+分子 #19: RNA-directed RNA polymerase catalytic subunit
+分子 #20: Polymerase basic protein 2
+分子 #23: Likely SPT5 KOWx4-KOW5 linker, register unclear
+分子 #10: non-template DNA (43-mer)
+分子 #12: Template DNA (43-MER)
+分子 #11: cap(1)-RNA (35-MER)
+分子 #21: 3' vRNA
+分子 #22: 5'-vRNA
+分子 #24: ZINC ION
+分子 #25: 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-(2'-O-METHYL)-GUANOSINE
+分子 #26: MAGNESIUM ION
+分子 #27: PHOSPHATE ION
-実験情報
-構造解析
| 手法 | クライオ電子顕微鏡法 |
|---|---|
解析 | 単粒子再構成法 |
| 試料の集合状態 | particle |
-
試料調製
| 緩衝液 | pH: 8 詳細: 20 mM BICINE pH 8.0 150 mM NaCl 0.1 mM MgCl2 4% glycerol |
|---|---|
| 凍結 | 凍結剤: ETHANE / チャンバー内湿度: 95 % / 装置: FEI VITROBOT MARK IV |
-
電子顕微鏡法
| 顕微鏡 | TFS KRIOS |
|---|---|
| 撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均露光時間: 1.95 sec. / 平均電子線量: 39.94 e/Å2 |
| 電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
| 電子光学系 | 照射モード: OTHER / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.0 µm / 最小 デフォーカス(公称値): 0.2 µm |
| 実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
ムービー
コントローラー
万見について




キーワード
Homo sapiens (ヒト)
Influenza B virus (B型インフルエンザウイルス)
データ登録者
ドイツ, European Union, 2件
引用

















Z (Sec.)
Y (Row.)
X (Col.)





































































































































Trichoplusia ni (イラクサキンウワバ)

解析
FIELD EMISSION GUN
