dynein (ダイニン) / cilia (繊毛) / intraflagellar transport / complex / motor protein (モータータンパク質)
機能・相同性
機能・相同性情報
intraciliary transport involved in cilium assembly / nitric-oxide synthase inhibitor activity / negative regulation of DNA strand resection involved in replication fork processing / intraciliary retrograde transport / visual behavior / organelle organization / methylated-DNA-[protein]-cysteine S-methyltransferase / methylated-DNA-[protein]-cysteine S-methyltransferase activity / intraciliary transport / ciliary transition zone ...intraciliary transport involved in cilium assembly / nitric-oxide synthase inhibitor activity / negative regulation of DNA strand resection involved in replication fork processing / intraciliary retrograde transport / visual behavior / organelle organization / methylated-DNA-[protein]-cysteine S-methyltransferase / methylated-DNA-[protein]-cysteine S-methyltransferase activity / intraciliary transport / ciliary transition zone / dynein light chain binding / regulation of cilium assembly / motile cilium assembly / dynein heavy chain binding / Activation of BIM and translocation to mitochondria / embryonic skeletal system morphogenesis / ciliary tip / DNA modification / Intraflagellar transport / negative regulation of nitric oxide biosynthetic process / cell projection organization / dynein complex / negative regulation of phosphorylation / COPI-independent Golgi-to-ER retrograde traffic / minus-end-directed microtubule motor activity / cytoplasmic dynein complex / dynein light intermediate chain binding / ciliary plasm / 繊毛 / enzyme inhibitor activity / determination of left/right symmetry / microtubule motor activity / ciliary base / dynein intermediate chain binding / オートファジー / 中心体マトリックス / microtubule-based movement / axoneme / positive regulation of insulin secretion involved in cellular response to glucose stimulus / tertiary granule membrane / ficolin-1-rich granule membrane / cilium assembly / spermatid development / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / COPI-mediated anterograde transport / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / axon cytoplasm / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of mitotic centrosome proteins and complexes / Resolution of Sister Chromatid Cohesion / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / substantia nigra development / MHC class II antigen presentation / 中心小体 / AURKA Activation by TPX2 / ciliary basal body / filopodium / RHO GTPases Activate Formins / 繊毛 / 紡錘体 / 動原体 / Aggrephagy / HCMV Early Events / Separation of Sister Chromatids / Regulation of PLK1 Activity at G2/M Transition / apical part of cell / site of double-strand break / メチル化 / scaffold protein binding / 微小管 / 細胞骨格 / DNA修復 / 中心体 / apoptotic process / Neutrophil degranulation / protein-containing complex binding / ゴルジ体 / enzyme binding / ATP hydrolysis activity / ミトコンドリア / DNA binding / extracellular space / ATP binding / 生体膜 / identical protein binding / metal ion binding / 細胞核 / 細胞膜 / 細胞質基質 / 細胞質 類似検索 - 分子機能
Cytoplasmic dynein 2 light intermediate chain 1 / Cytoplasmic dynein 2 intermediate chain 1 / : / Cytoplasmic dynein 2 heavy chain 1, AAA+ ATPase domain / Methylguanine DNA methyltransferase, ribonuclease-like domain / Dynein family light intermediate chain / 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain / Dynein light intermediate chain (DLIC) / Dynein light chain roadblock-type 1/2 / Methylated DNA-protein cysteine methyltransferase domain superfamily ...Cytoplasmic dynein 2 light intermediate chain 1 / Cytoplasmic dynein 2 intermediate chain 1 / : / Cytoplasmic dynein 2 heavy chain 1, AAA+ ATPase domain / Methylguanine DNA methyltransferase, ribonuclease-like domain / Dynein family light intermediate chain / 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain / Dynein light intermediate chain (DLIC) / Dynein light chain roadblock-type 1/2 / Methylated DNA-protein cysteine methyltransferase domain superfamily / Methylated-DNA-[protein]-cysteine S-methyltransferase, active site / Methylated-DNA--protein-cysteine methyltransferase active site. / Methylated-DNA-[protein]-cysteine S-methyltransferase, DNA binding / Methylated DNA-protein cysteine methyltransferase, DNA binding domain / 6-O-methylguanine DNA methyltransferase, DNA binding domain / Dynein light chain, type 1/2, conserved site / Dynein light chain type 1 signature. / Dynein light chain type 1 / Dynein light chain, type 1/2 / Dynein light chain superfamily / Dynein light chain type 1 / Roadblock/LAMTOR2 domain / Roadblock/LC7 domain / Roadblock/LC7 domain / Dynein heavy chain, C-terminal domain / Dynein heavy chain, C-terminal domain, barrel region / Dynein heavy chain C-terminal domain / P-loop containing dynein motor region / Dynein heavy chain, tail / Dynein heavy chain, N-terminal region 1 / Dynein heavy chain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, linker / Dynein heavy chain, AAA module D4 / Dynein heavy chain, coiled coil stalk / Dynein heavy chain, hydrolytic ATP-binding dynein motor region / Dynein heavy chain, ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Dynein heavy chain AAA lid domain superfamily / Dynein heavy chain, domain 2, N-terminal / Dynein heavy chain, linker, subdomain 3 / Dynein heavy chain, AAA1 domain, small subdomain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, N-terminal region 2 / Hydrolytic ATP binding site of dynein motor region / Microtubule-binding stalk of dynein motor / P-loop containing dynein motor region D4 / ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40リピート / WD40リピート / WD40-repeat-containing domain superfamily / Winged helix-like DNA-binding domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性
Biotechnology and Biological Sciences Research Council
BB/P008348/1
英国
Royal Society
RG170260
英国
Wellcome Trust
WT100387
英国
Medical Research Council (United Kingdom)
MC_UP_A025_1011
英国
Wellcome Trust
079605/Z/06/Z
英国
Biotechnology and Biological Sciences Research Council
BB/L014211/1
英国
引用
ジャーナル: Nat Struct Mol Biol / 年: 2019 タイトル: Structure of the dynein-2 complex and its assembly with intraflagellar transport trains. 著者: Katerina Toropova / Ruta Zalyte / Aakash G Mukhopadhyay / Miroslav Mladenov / Andrew P Carter / Anthony J Roberts / 要旨: Dynein-2 assembles with polymeric intraflagellar transport (IFT) trains to form a transport machinery that is crucial for cilia biogenesis and signaling. Here we recombinantly expressed the ~1.4-MDa ...Dynein-2 assembles with polymeric intraflagellar transport (IFT) trains to form a transport machinery that is crucial for cilia biogenesis and signaling. Here we recombinantly expressed the ~1.4-MDa human dynein-2 complex and solved its cryo-EM structure to near-atomic resolution. The two identical copies of the dynein-2 heavy chain are contorted into different conformations by a WDR60-WDR34 heterodimer and a block of two RB and six LC8 light chains. One heavy chain is steered into a zig-zag conformation, which matches the periodicity of the anterograde IFT-B train. Contacts between adjacent dyneins along the train indicate a cooperative mode of assembly. Removal of the WDR60-WDR34-light chain subcomplex renders dynein-2 monomeric and relieves autoinhibition of its motility. Our results converge on a model in which an unusual stoichiometry of non-motor subunits controls dynein-2 assembly, asymmetry, and activity, giving mechanistic insight into the interaction of dynein-2 with IFT trains and the origin of diverse functions in the dynein family.
フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 検出モード: COUNTING / 平均電子線量: 49.6 e/Å2 詳細: Average electron dose per image (e-/A2) for additional datasets was 46.8 and 45.4
詳細: EMD-3707 map was low pass filtered to 60A and used as a reference model for 3D classification and reconstruction of an initial, in-house collected cryo-EM dataset (Polara 300kV instrument). ...詳細: EMD-3707 map was low pass filtered to 60A and used as a reference model for 3D classification and reconstruction of an initial, in-house collected cryo-EM dataset (Polara 300kV instrument). This initial map was then used as a reference model for the dataset described in this entry.
初期 角度割当
タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION (ver. 2.1)
最終 角度割当
タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION (ver. 2.1)