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- EMDB-4906: Cryo-EM reconstruction of TssA protein from T6SS of Escherichia coli. -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-4906 | |||||||||
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Title | Cryo-EM reconstruction of TssA protein from T6SS of Escherichia coli. | |||||||||
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Function / homology | Type VI secretion system-associated, VCA0119 / Type VI secretion, EvfE, EvfF, ImpA, BimE, VC_A0119, VasJ / ImpA, N-terminal / ImpA, N-terminal, type VI secretion system / Putative type VI secretion protein![]() | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.2 Å | |||||||||
![]() | Nazarov S / Shneider M / Basler M / Leiman P | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structure of TssA protein from Type VI secretion system of E. coli. Authors: Nazarov S / Demurtas D / Shneider M / Basler M / Leiman P | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 100.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 19.7 KB 19.7 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 7.8 KB | Display | ![]() |
Images | ![]() | 144 KB | ||
Masks | ![]() | 129.7 MB | ![]() | |
Others | ![]() ![]() ![]() ![]() | 37.8 MB 120.5 MB 95.6 MB 97.2 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 401.1 KB | Display | ![]() |
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Full document | ![]() | 400.2 KB | Display | |
Data in XML | ![]() | 16 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6rjuMUC M: atomic model generated by this map U: unfit; in different coordinate system*YM C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 1.058 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
File | ![]() | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Additional map: #1
File | emd_4906_additional_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Additional map: #2
File | emd_4906_additional_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_4906_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_4906_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : C-terminal domain of T6SS protein TssA.
Entire | Name: C-terminal domain of T6SS protein TssA. |
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Components |
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-Supramolecule #1: C-terminal domain of T6SS protein TssA.
Supramolecule | Name: C-terminal domain of T6SS protein TssA. / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() |
Recombinant expression | Organism: ![]() ![]() |
Molecular weight | Theoretical: 634 KDa |
-Macromolecule #1: Putative type VI secretion protein
Macromolecule | Name: Putative type VI secretion protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 59.159789 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MASIHSLLSA CQTTPRDVAE PAQVRIALWD KWLAPVTPDN PAGDDAGYDD DFQQMREEVN KLSGADAGIV SQLAEKLLTT RTKDIRVAT WYIWARLRQD GEKGLADGLE LLTGLLQRFG EHLHPQRSRA RKAALEWLCS ARILDSLSLY PEVVKADTLR I AGALWLAE ...String: MASIHSLLSA CQTTPRDVAE PAQVRIALWD KWLAPVTPDN PAGDDAGYDD DFQQMREEVN KLSGADAGIV SQLAEKLLTT RTKDIRVAT WYIWARLRQD GEKGLADGLE LLTGLLQRFG EHLHPQRSRA RKAALEWLCS ARILDSLSLY PEVVKADTLR I AGALWLAE QTFTDEASAP VLNGLYQALE NRLMKAGGVD AVVPQEAAAP APTVTSGSVM ALSAITSGQE LLSQARVLAK YL RDQPEGW LAAHRLMKSV RHDTLHQLPP LSADGRTRIA PPGPDRRASL KRLYLQQNWL SLLEQCDDMF ARGASHLWLD LQW YIHQAL LQTGKENYAA IIQYDLKGLL LRLPGLETLA FNDGMPFADD VTLSWIQQQV MECGERWAEE PSVTITAAPG DNDI LSLEP EALQIADNEG TEAALSWLQA RPGIQSDRSN WLLRLL(MSE)ARV AEQTGKNDLA LHLLAELDER ATRLTLSQWE P ELVFEVKA RRLKLLR(MSE)KS AKTESDRVRL QPD(MSE)EHLLAG LIAIDAARAA VLCNSGSS |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE / Chamber humidity: 90 % / Chamber temperature: 293 K / Details: Leica EM GP2. |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Specialist optics | Energy filter - Name: GIF Quantum LS |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Number real images: 3269 / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |