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- EMDB-4665: Structure of a human fully-assembled precatalytic spliceosome (pr... -

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Basic information

Entry
Database: EMDB / ID: EMD-4665
TitleStructure of a human fully-assembled precatalytic spliceosome (pre-B complex).
Map dataNone
Sample
  • Complex: Fully-assembled precatalytic spliceosome (pre-B complex).
    • Complex: Fully-assembled precatalytic spliceosome (pre-B complex).
      • RNA: x 5 types
      • Protein or peptide: x 43 types
    • Complex: Probable ATP-dependent RNA helicase DDX23
      • Protein or peptide: x 1 types
    • Complex: AdML pre-mRNA
      • RNA: x 1 types
  • Ligand: x 4 types
Function / homology
Function and homology information


mRNA Splicing - Minor Pathway / negative regulation of protein refolding / mRNA Splicing - Major Pathway / Lsm2-8 complex / U6 snRNA 3'-end binding / spliceosomal snRNP complex / ribonucleoprotein complex localization / snRNP binding / U4atac snRNP / positive regulation of cytotoxic T cell differentiation ...mRNA Splicing - Minor Pathway / negative regulation of protein refolding / mRNA Splicing - Major Pathway / Lsm2-8 complex / U6 snRNA 3'-end binding / spliceosomal snRNP complex / ribonucleoprotein complex localization / snRNP binding / U4atac snRNP / positive regulation of cytotoxic T cell differentiation / maturation of 5S rRNA / RNA localization / R-loop processing / U4atac snRNA binding / regulation of ATP-dependent activity / mRNA decay by 5' to 3' exoribonuclease / Lsm1-7-Pat1 complex / regulation of mitotic cell cycle spindle assembly checkpoint / U11/U12 snRNP / U6 snRNP / box C/D sno(s)RNA binding / PH domain binding / U2 snRNP binding / U7 snRNA binding / histone pre-mRNA DCP binding / U7 snRNP / dense fibrillar component / B-WICH complex / histone pre-mRNA 3'end processing complex / cis assembly of pre-catalytic spliceosome / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / splicing factor binding / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / protein methylation / U12-type spliceosomal complex / methylosome / P-body assembly / U4/U6 snRNP / 7-methylguanosine cap hypermethylation / U1 snRNP binding / pICln-Sm protein complex / U2-type catalytic step 1 spliceosome / RNA splicing, via transesterification reactions / small nuclear ribonucleoprotein complex / sno(s)RNA-containing ribonucleoprotein complex / SMN-Sm protein complex / spliceosomal tri-snRNP complex / P granule / U4 snRNA binding / telomerase holoenzyme complex / box C/D methylation guide snoRNP complex / negative regulation of chaperone-mediated autophagy / U2-type spliceosomal complex / mRNA cis splicing, via spliceosome / U2-type precatalytic spliceosome / commitment complex / telomerase RNA binding / U2-type prespliceosome assembly / U2-type catalytic step 2 spliceosome / U4 snRNP / box C/D snoRNP assembly / U2 snRNP / SAGA complex / positive regulation of mRNA splicing, via spliceosome / rRNA modification in the nucleus and cytosol / RNA Polymerase II Transcription Termination / positive regulation of transcription by RNA polymerase III / tRNA processing / U1 snRNP / U3 snoRNA binding / pre-mRNA 5'-splice site binding / nuclear-transcribed mRNA catabolic process / U2-type prespliceosome / K63-linked polyubiquitin modification-dependent protein binding / nuclear androgen receptor binding / positive regulation of transcription by RNA polymerase I / precatalytic spliceosome / spliceosomal complex assembly / mRNA Splicing - Minor Pathway / regulation of alternative mRNA splicing, via spliceosome / mRNA catabolic process / negative regulation of mRNA splicing, via spliceosome / mRNA 5'-splice site recognition / regulation of RNA splicing / mRNA 3'-splice site recognition / MLL1 complex / spliceosomal tri-snRNP complex assembly / single fertilization / Major pathway of rRNA processing in the nucleolus and cytosol / U5 snRNA binding / U5 snRNP / RNA processing / U2 snRNA binding / U6 snRNA binding / regulation of DNA repair / spliceosomal snRNP assembly / Cajal body / ribonucleoprotein complex binding / pre-mRNA intronic binding
Similarity search - Function
Pre-mRNA-processing factor 6/Prp1/STA1 / snRNP70, RNA recognition motif / U4/U6.U5 small nuclear ribonucleoprotein 27kDa protein / RBM42, RNA recognition motif / U4/U6.U5 small nuclear ribonucleoproteins / Serine/threonine-protein PRP4, catalytic domain / U1 small nuclear ribonucleoprotein C / U1 small nuclear ribonucleoprotein of 70kDa N-terminal / U1 small nuclear ribonucleoprotein of 70kDa MW N terminal / USP39 ...Pre-mRNA-processing factor 6/Prp1/STA1 / snRNP70, RNA recognition motif / U4/U6.U5 small nuclear ribonucleoprotein 27kDa protein / RBM42, RNA recognition motif / U4/U6.U5 small nuclear ribonucleoproteins / Serine/threonine-protein PRP4, catalytic domain / U1 small nuclear ribonucleoprotein C / U1 small nuclear ribonucleoprotein of 70kDa N-terminal / U1 small nuclear ribonucleoprotein of 70kDa MW N terminal / USP39 / U1-C, C2H2-type zinc finger / U1 zinc finger / PRP4-like superfamily / pre-mRNA processing factor 4 (PRP4) like / SF3B4, RNA recognition motif 2 / SNU66/SART1 family / HIND motif / SART-1 family / HIND motif / Splicing factor SF3a60 binding domain / Splicing factor SF3a60 binding domain / PWI domain superfamily / PWI domain / PWI domain profile. / Sm-like protein Lsm8 / : / Replication stress response SDE2 C-terminal / Cactus-binding C-terminus of cactin protein / U6 snRNA-associated Sm-like protein Lsm1/8 / Pre-mRNA processing factor 4 (PRP4)-like / U6 snRNA-associated Sm-like protein LSm2 / Sm-like protein Lsm4 / Splicing factor 3A subunit 1, ubiquitin domain / Splicing Factor Motif, present in Prp18 and Pr04 / Sm-like protein Lsm7 / Sm-like protein LSm5 / Sm-like protein Lsm3 / U6 snRNA-associated Sm-like protein Lsm3 / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 2 / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 1 / Pre-mRNA-splicing factor 3 / U4/U6 small nuclear ribonucleoprotein Prp3 / pre-mRNA processing factor 3 domain / Prp31 C-terminal / U4/U6 small nuclear ribonucleoprotein Prp31 / Prp31 C terminal domain / Small nuclear ribonucleoprotein Prp3, C-terminal domain / SF3A2 domain / Small nuclear ribonucleoprotein Prp3, C-terminal domain / Pre-mRNA-splicing factor SF3a complex subunit 2 (Prp11) / PWI domain / Splicing factor SF3a60 /Prp9 subunit, C-terminal / SF3A3 domain / SF3B4, RNA recognition motif 1 / SF3a60/Prp9 C-terminal / Pre-mRNA-splicing factor SF3A3, of SF3a complex, Prp9 / PWI, domain in splicing factors / : / Dim1 family / Mitosis protein DIM1 / Mitosis protein DIM1 / Splicing factor 3B, subunit 5 / PRP1 splicing factor, N-terminal / PRP1 splicing factor, N-terminal / Splicing factor 3A subunit 1 / U1 small nuclear ribonucleoprotein A, RNA recognition motif 2 / Splicing factor 3B subunit 1 / Splicing factor 3B subunit 1 / Splicing factor 3A subunit 1, conserved domain / Pre-mRNA splicing factor PRP21 like protein / Domain of unknown function DUF382 / Domain of unknown function (DUF382) / U1 small nuclear ribonucleoprotein A, RNA recognition motif 1 / SWAP/Surp / SWAP/Surp superfamily / Surp module / Zinc-finger of C2H2 type / SURP motif repeat profile. / Suppressor-of-White-APricot splicing regulator / PHF5-like / PHF5-like protein / PSP, proline-rich / PSP / proline-rich domain in spliceosome associated proteins / Splicing factor 3B subunit 5/RDS3 complex subunit 10 / Splicing factor 3B subunit 10 (SF3b10) / Small ribonucleoprotein associated, SmB/SmN / Splicing factor 3B subunit 1-like / Matrin/U1-C, C2H2-type zinc finger / Zinc finger matrin-type profile. / Small nuclear ribonucleoprotein D1 / U2A'/phosphoprotein 32 family A, C-terminal / occurring C-terminal to leucine-rich repeats / Leucine-rich repeat / Brr2, N-terminal helicase PWI domain / : / N-terminal helicase PWI domain / Pre-mRNA-splicing helicase BRR2 plug domain / H/ACA ribonucleoprotein complex, subunit Nhp2-like / SAP domain
Similarity search - Domain/homology
U4/U6 small nuclear ribonucleoprotein Prp4 / U4/U6.U5 tri-snRNP-associated protein 1 / U4/U6 small nuclear ribonucleoprotein Prp3 / Splicing factor 3B subunit 1 / U5 small nuclear ribonucleoprotein 200 kDa helicase / U6 snRNA-associated Sm-like protein LSm8 / U2 small nuclear ribonucleoprotein B'' / U1 small nuclear ribonucleoprotein 70 kDa / U1 small nuclear ribonucleoprotein A / U1 small nuclear ribonucleoprotein C ...U4/U6 small nuclear ribonucleoprotein Prp4 / U4/U6.U5 tri-snRNP-associated protein 1 / U4/U6 small nuclear ribonucleoprotein Prp3 / Splicing factor 3B subunit 1 / U5 small nuclear ribonucleoprotein 200 kDa helicase / U6 snRNA-associated Sm-like protein LSm8 / U2 small nuclear ribonucleoprotein B'' / U1 small nuclear ribonucleoprotein 70 kDa / U1 small nuclear ribonucleoprotein A / U1 small nuclear ribonucleoprotein C / U2 small nuclear ribonucleoprotein A' / Small nuclear ribonucleoprotein-associated proteins B and B' / NHP2-like protein 1 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein G / U6 snRNA-associated Sm-like protein LSm3 / U6 snRNA-associated Sm-like protein LSm6 / Small nuclear ribonucleoprotein Sm D1 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / Thioredoxin-like protein 4A / Splicing factor 3A subunit 3 / Splicing factor 3B subunit 2 / Serine/threonine-protein kinase PRP4 homolog / 116 kDa U5 small nuclear ribonucleoprotein component / Splicing factor 3B subunit 3 / Splicing factor 3B subunit 4 / Splicing factor 3A subunit 2 / Splicing factor 3A subunit 1 / Ubiquitin carboxyl-terminal hydrolase 39 / Pre-mRNA-processing-splicing factor 8 / PHD finger-like domain-containing protein 5A / U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein / U4/U6 small nuclear ribonucleoprotein Prp31 / Pre-mRNA-processing factor 6 / U5 small nuclear ribonucleoprotein 40 kDa protein / RNA-binding protein 42 / Probable ATP-dependent RNA helicase DDX23 / Splicing factor 3B subunit 5 / U6 snRNA-associated Sm-like protein LSm7 / U6 snRNA-associated Sm-like protein LSm2 / U6 snRNA-associated Sm-like protein LSm5 / U6 snRNA-associated Sm-like protein LSm4
Similarity search - Component
Biological speciesHomo sapiens (human) / Human adenovirus 2 / Human (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.28 Å
AuthorsCharenton C / Wilkinson ME / Nagai K
Funding support United Kingdom, 2 items
OrganizationGrant numberCountry
European Research CouncilAdG-693087-SPLICE3D United Kingdom
Medical Research Council (United Kingdom)MC_U105184330 United Kingdom
CitationJournal: Science / Year: 2019
Title: Mechanism of 5' splice site transfer for human spliceosome activation.
Authors: Clément Charenton / Max E Wilkinson / Kiyoshi Nagai /
Abstract: The prespliceosome, comprising U1 and U2 small nuclear ribonucleoproteins (snRNPs) bound to the precursor messenger RNA 5' splice site (5'SS) and branch point sequence, associates with the U4/U6.U5 ...The prespliceosome, comprising U1 and U2 small nuclear ribonucleoproteins (snRNPs) bound to the precursor messenger RNA 5' splice site (5'SS) and branch point sequence, associates with the U4/U6.U5 tri-snRNP to form the fully assembled precatalytic pre-B spliceosome. Here, we report cryo-electron microscopy structures of the human pre-B complex captured before U1 snRNP dissociation at 3.3-angstrom core resolution and the human tri-snRNP at 2.9-angstrom resolution. U1 snRNP inserts the 5'SS-U1 snRNA helix between the two RecA domains of the Prp28 DEAD-box helicase. Adenosine 5'-triphosphate-dependent closure of the Prp28 RecA domains releases the 5'SS to pair with the nearby U6 ACAGAGA-box sequence presented as a mobile loop. The structures suggest that formation of the 5'SS-ACAGAGA helix triggers remodeling of an intricate protein-RNA network to induce Brr2 helicase relocation to its loading sequence in U4 snRNA, enabling Brr2 to unwind the U4/U6 snRNA duplex to allow U6 snRNA to form the catalytic center of the spliceosome.
History
DepositionMar 7, 2019-
Header (metadata) releaseApr 17, 2019-
Map releaseApr 17, 2019-
UpdateOct 7, 2020-
Current statusOct 7, 2020Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
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  • Surface view colored by radius
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  • Surface view with fitted model
  • Atomic models: PDB-6qx9
  • Surface level: 0.01
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6qx9
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_4665.png

Downloads & links

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Map

FileDownload / File: emd_4665.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationNone
Voxel sizeX=Y=Z: 1.022 Å
Density
Contour LevelBy AUTHOR: 0.01 / Movie #1: 0.01
Minimum - Maximum-0.047622178 - 0.09746684
Average (Standard dev.)0.0000085074 (±0.0032210834)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions420420420
Spacing420420420
CellA=B=C: 429.24 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0221.0221.022
M x/y/z420420420
origin x/y/z0.0000.0000.000
length x/y/z429.240429.240429.240
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS420420420
D min/max/mean-0.0480.0970.000

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Supplemental data

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Sample components

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Entire : Fully-assembled precatalytic spliceosome (pre-B complex).

EntireName: Fully-assembled precatalytic spliceosome (pre-B complex).
Components
  • Complex: Fully-assembled precatalytic spliceosome (pre-B complex).
    • Complex: Fully-assembled precatalytic spliceosome (pre-B complex).
      • RNA: U1 snRNA
      • RNA: U6 snRNA
      • Protein or peptide: U5 small nuclear ribonucleoprotein 40 kDa protein
      • Protein or peptide: Splicing factor 3B subunit 4
      • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
      • Protein or peptide: U4/U6 small nuclear ribonucleoprotein Prp4
      • Protein or peptide: Small nuclear ribonucleoprotein E
      • Protein or peptide: U1 small nuclear ribonucleoprotein 70 kDa
      • Protein or peptide: U4/U6 small nuclear ribonucleoprotein Prp31
      • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
      • Protein or peptide: RNA-binding protein 42
      • Protein or peptide: Small nuclear ribonucleoprotein F
      • Protein or peptide: U6 snRNA-associated Sm-like protein LSm6
      • Protein or peptide: Small nuclear ribonucleoprotein Sm D2
      • Protein or peptide: U6 snRNA-associated Sm-like protein LSm7
      • Protein or peptide: U6 snRNA-associated Sm-like protein LSm2
      • Protein or peptide: U2 small nuclear ribonucleoprotein B''
      • Protein or peptide: Splicing factor 3A subunit 2
      • Protein or peptide: Splicing factor 3B subunit 2
      • Protein or peptide: 116 kDa U5 small nuclear ribonucleoprotein component
      • Protein or peptide: U1 small nuclear ribonucleoprotein A
      • Protein or peptide: U4/U6.U5 tri-snRNP-associated protein 1
      • Protein or peptide: Pre-mRNA-processing factor 6
      • Protein or peptide: NHP2-like protein 1
      • Protein or peptide: U6 snRNA-associated Sm-like protein LSm3
      • RNA: U2 snRNA
      • Protein or peptide: Splicing factor 3B subunit 3
      • Protein or peptide: Small nuclear ribonucleoprotein G
      • Protein or peptide: U6 snRNA-associated Sm-like protein LSm8
      • Protein or peptide: Pre-mRNA-processing-splicing factor 8
      • Protein or peptide: Splicing factor 3A subunit 3
      • Protein or peptide: U4/U6.U5 tri-snRNP-associated protein 2
      • Protein or peptide: Thioredoxin-like protein 4A
      • Protein or peptide: U6 snRNA-associated Sm-like protein LSm4
      • Protein or peptide: PHD finger-like domain-containing protein 5A
      • Protein or peptide: U1 small nuclear ribonucleoprotein C
      • Protein or peptide: Serine/threonine-protein kinase PRP4 homolog
      • Protein or peptide: U4/U6 small nuclear ribonucleoprotein Prp3
      • RNA: U4 snRNA
      • Protein or peptide: U2 small nuclear ribonucleoprotein A'
      • Protein or peptide: Splicing factor 3A subunit 1,Splicing factor 3A subunit 1,Splicing factor 3A subunit 1
      • Protein or peptide: U6 snRNA-associated Sm-like protein LSm5
      • Protein or peptide: U5 small nuclear ribonucleoprotein 200 kDa helicase
      • Protein or peptide: Splicing factor 3B subunit 1
      • Protein or peptide: Small nuclear ribonucleoprotein-associated proteins B and B'
      • Protein or peptide: Splicing factor 3B subunit 5
      • Protein or peptide: U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein
      • RNA: U5 snRNA
    • Complex: Probable ATP-dependent RNA helicase DDX23
      • Protein or peptide: Probable ATP-dependent RNA helicase DDX23
    • Complex: AdML pre-mRNA
      • RNA: AdML pre-mRNA
  • Ligand: ZINC ION
  • Ligand: MAGNESIUM ION
  • Ligand: GUANOSINE-5'-TRIPHOSPHATE
  • Ligand: INOSITOL HEXAKISPHOSPHATE

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Supramolecule #1: Fully-assembled precatalytic spliceosome (pre-B complex).

SupramoleculeName: Fully-assembled precatalytic spliceosome (pre-B complex).
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#50
Molecular weightTheoretical: 3.0 MDa

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Supramolecule #2: Fully-assembled precatalytic spliceosome (pre-B complex).

SupramoleculeName: Fully-assembled precatalytic spliceosome (pre-B complex).
type: complex / ID: 2 / Parent: 1
Macromolecule list: #1-#7, #9-#14, #16, #18-#23, #27-#50, #25-#26, #15, #17
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #3: Probable ATP-dependent RNA helicase DDX23

SupramoleculeName: Probable ATP-dependent RNA helicase DDX23 / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #24
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)

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Supramolecule #4: AdML pre-mRNA

SupramoleculeName: AdML pre-mRNA / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #8
Source (natural)Organism: Human adenovirus 2
Recombinant expressionOrganism: synthetic construct (others)

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Macromolecule #1: U1 snRNA

MacromoleculeName: U1 snRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 52.69707 KDa
SequenceString:
AUACUUACCU GGCAGGGGAG AUACCAUGAU CACGAAGGUG GUUUUCCCAG GGCGAGGCUU AUCCAUUGCA CUCCGGAUGU GCUGACCCC UGCGAUUUCC CCAAAUGUGG GAAACUCGAC UGCAUAAUUU GUGGUAGUGG GGGACUGCGU UCGCGCUUUC C CCUG

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Macromolecule #2: U6 snRNA

MacromoleculeName: U6 snRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 34.09827 KDa
SequenceString:
GUGCUCGCUU CGGCAGCACA UAUACUAAAA UUGGAACGAU ACAGAGAAGA UUAGCAUGGC CCCUGCGCAA GGAUGACACG CAAAUUCGU GAAGCGUUCC AUAUUUU

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Macromolecule #8: AdML pre-mRNA

MacromoleculeName: AdML pre-mRNA / type: rna / ID: 8 / Number of copies: 1
Source (natural)Organism: Human adenovirus 2
Molecular weightTheoretical: 15.279272 KDa
SequenceString:
GGGUGAGUAC (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N) (N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)GC UUUCGUGCUG ACC

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Macromolecule #17: U5 snRNA

MacromoleculeName: U5 snRNA / type: rna / ID: 17 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 37.254855 KDa
SequenceString:
AUACUCUGGU UUCUCUUCAG AUCGCAUAAA UCUUUCGCCU UUUACUAAAG AUUUCCGUGG AGAGGAACAA CUCUGAGUCU UAACCCAAU UUUUUGAGGC CUUGCUUUGG CAAGGCUA

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Macromolecule #32: U2 snRNA

MacromoleculeName: U2 snRNA / type: rna / ID: 32 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 60.170445 KDa
SequenceString:
AUCGCUUCUC GGCCUUUUGG CUAAGAUCAA GUGUAGUAUC UGUUCUUAUC AGUUUAAUAU CUGAUACGUC CUCUAUCCGA GGACAAUAU AUUAAAUAGA UUUUUGGAGC AGGGAGAUGG AAUAGGAGCU UGCUCCGUCC ACUCCACGCA UCGACCUGGU A UUGCAGUA CCUCCAGGAA CGGUGCACCC

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Macromolecule #45: U4 snRNA

MacromoleculeName: U4 snRNA / type: rna / ID: 45 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 46.997742 KDa
SequenceString:
(M7M)AGCUUUGCG CAGUGGCAGU AUCGUAGCCA AUGAGGUUUA UCCGAGGCGC GAUUAUUGCU AAUUGAAAAC UUUUCC CAA UACCCCGCCG UGACGACUUG CAAUAUAGUC GGCAUUGGCA AUUUUUGACA GUCUCUACGG AGACUGG

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Macromolecule #3: U5 small nuclear ribonucleoprotein 40 kDa protein

MacromoleculeName: U5 small nuclear ribonucleoprotein 40 kDa protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 39.359492 KDa
SequenceString: MIEQQKRKGP ELPLVPVKRQ RHELLLGAGS GPGAGQQQAT PGALLQAGPP RCSSLQAPIM LLSGHEGEVY CCKFHPNGST LASAGFDRL ILLWNVYGDC DNYATLKGHS GAVMELHYNT DGSMLFSAST DKTVAVWDSE TGERVKRLKG HTSFVNSCYP A RRGPQLVC ...String:
MIEQQKRKGP ELPLVPVKRQ RHELLLGAGS GPGAGQQQAT PGALLQAGPP RCSSLQAPIM LLSGHEGEVY CCKFHPNGST LASAGFDRL ILLWNVYGDC DNYATLKGHS GAVMELHYNT DGSMLFSAST DKTVAVWDSE TGERVKRLKG HTSFVNSCYP A RRGPQLVC TGSDDGTVKL WDIRKKAAIQ TFQNTYQVLA VTFNDTSDQI ISGGIDNDIK VWDLRQNKLT YTMRGHADSV TG LSLSSEG SYLLSNAMDN TVRVWDVRPF APKERCVKIF QGNVHNFEKN LLRCSWSPDG SKIAAGSADR FVYVWDTTSR RIL YKLPGH AGSINEVAFH PDEPIIISAS SDKRLYMGEI Q

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Macromolecule #4: Splicing factor 3B subunit 4

MacromoleculeName: Splicing factor 3B subunit 4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 44.43657 KDa
SequenceString: MAAGPISERN QDATVYVGGL DEKVSEPLLW ELFLQAGPVV NTHMPKDRVT GQHQGYGFVE FLSEEDADYA IKIMNMIKLY GKPIRVNKA SAHNKNLDVG ANIFIGNLDP EIDEKLLYDT FSAFGVILQT PKIMRDPDTG NSKGYAFINF ASFDASDAAI E AMNGQYLC ...String:
MAAGPISERN QDATVYVGGL DEKVSEPLLW ELFLQAGPVV NTHMPKDRVT GQHQGYGFVE FLSEEDADYA IKIMNMIKLY GKPIRVNKA SAHNKNLDVG ANIFIGNLDP EIDEKLLYDT FSAFGVILQT PKIMRDPDTG NSKGYAFINF ASFDASDAAI E AMNGQYLC NRPITVSYAF KKDSKGERHG SAAERLLAAQ NPLSQADRPH QLFADAPPPP SAPNPVVSSL GSGLPPPGMP PP GSFPPPV PPPGALPPGI PPAMPPPPMP PGAAGHGPPS AGTPGAGHPG HGHSHPHPFP PGGMPHPGMS QMQLAHHGPH GLG HPHAGP PGSGGQPPPR PPPGMPHPGP PPMGMPPRGP PFGSPMGHPG PMPPHGMRGP PPLMPPHGYT GPPRPPPYGY QRGP LPPPR PTPRPPVPPR GPLRGPLPQ

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Macromolecule #5: Small nuclear ribonucleoprotein Sm D3

MacromoleculeName: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 5 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 13.940308 KDa
SequenceString:
MSIGVPIKVL HEAEGHIVTC ETNTGEVYRG KLIEAEDNMN CQMSNITVTY RDGRVAQLEQ VYIRGSKIRF LILPDMLKNA PMLKSMKNK NQGSGAGRGK AAILKAQVAA RGRGRGMGRG NIFQKRR

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Macromolecule #6: U4/U6 small nuclear ribonucleoprotein Prp4

MacromoleculeName: U4/U6 small nuclear ribonucleoprotein Prp4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 58.536105 KDa
SequenceString: MASSRASSTQ ATKTKAPDDL VAPVVKKPHI YYGSLEEKER ERLAKGESGI LGKDGLKAGI EAGNINITSG EVFEIEEHIS ERQAEVLAE FERRKRARQI NVSTDDSEVK ACLRALGEPI TLFGEGPAER RERLRNILSV VGTDALKKTK KDDEKSKKSK E EYQQTWYH ...String:
MASSRASSTQ ATKTKAPDDL VAPVVKKPHI YYGSLEEKER ERLAKGESGI LGKDGLKAGI EAGNINITSG EVFEIEEHIS ERQAEVLAE FERRKRARQI NVSTDDSEVK ACLRALGEPI TLFGEGPAER RERLRNILSV VGTDALKKTK KDDEKSKKSK E EYQQTWYH EGPNSLKVAR LWIANYSLPR AMKRLEEARL HKEIPETTRT SQMQELHKSL RSLNNFCSQI GDDRPISYCH FS PNSKMLA TACWSGLCKL WSVPDCNLLH TLRGHNTNVG AIVFHPKSTV SLDPKDVNLA SCAADGSVKL WSLDSDEPVA DIE GHTVRV ARVMWHPSGR FLGTTCYDRS WRLWDLEAQE EILHQEGHSM GVYDIAFHQD GSLAGTGGLD AFGRVWDLRT GRCI MFLEG HLKEIYGINF SPNGYHIATG SGDNTCKVWD LRQRRCVYTI PAHQNLVTGV KFEPIHGNFL LTGAYDNTAK IWTHP GWSP LKTLAGHEGK VMGLDISSDG QLIATCSYDR TFKLWMAE

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Macromolecule #7: Small nuclear ribonucleoprotein E

MacromoleculeName: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 7 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 10.817601 KDa
SequenceString:
MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKS RKQLGRIMLK GDNITLLQS VSN

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Macromolecule #9: U1 small nuclear ribonucleoprotein 70 kDa

MacromoleculeName: U1 small nuclear ribonucleoprotein 70 kDa / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 51.683184 KDa
SequenceString: MTQFLPPNLL ALFAPRDPIP YLPPLEKLPH EKHHNQPYCG IAPYIREFED PRDAPPPTRA ETREERMERK RREKIERRQQ EVETELKMW DPHNDPNAQG DAFKTLFVAR VNYDTTESKL RREFEVYGPI KRIHMVYSKR SGKPRGYAFI EYEHERDMHS A YKHADGKK ...String:
MTQFLPPNLL ALFAPRDPIP YLPPLEKLPH EKHHNQPYCG IAPYIREFED PRDAPPPTRA ETREERMERK RREKIERRQQ EVETELKMW DPHNDPNAQG DAFKTLFVAR VNYDTTESKL RREFEVYGPI KRIHMVYSKR SGKPRGYAFI EYEHERDMHS A YKHADGKK IDGRRVLVDV ERGRTVKGWR PRRLGGGLGG TRRGGADVNI RHSGRDDTSR YDERPGPSPL PHRDRDRDRE RE RRERSRE RDKERERRRS RSRDRRRRSR SRDKEERRRS RERSKDKDRD RKRRSSRSRE RARRERERKE ELRGGGGDMA EPS EAGDAP PDDGPPGELG PDGPDGPEEK GRDRDRERRR SHRSERERRR DRDRDRDRDR EHKRGERGSE RGRDEARGGG GGQD NGLEG LGNDSRDMYM ESEGGDGYLA PENGYLMEAA PE

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Macromolecule #10: U4/U6 small nuclear ribonucleoprotein Prp31

MacromoleculeName: U4/U6 small nuclear ribonucleoprotein Prp31 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 55.528969 KDa
SequenceString: MSLADELLAD LEEAAEEEEG GSYGEEEEEP AIEDVQEETQ LDLSGDSVKT IAKLWDSKMF AEIMMKIEEY ISKQAKASEV MGPVEAAPE YRVIVDANNL TVEIENELNI IHKFIRDKYS KRFPELESLV PNALDYIRTV KELGNSLDKC KNNENLQQIL T NATIMVVS ...String:
MSLADELLAD LEEAAEEEEG GSYGEEEEEP AIEDVQEETQ LDLSGDSVKT IAKLWDSKMF AEIMMKIEEY ISKQAKASEV MGPVEAAPE YRVIVDANNL TVEIENELNI IHKFIRDKYS KRFPELESLV PNALDYIRTV KELGNSLDKC KNNENLQQIL T NATIMVVS VTASTTQGQQ LSEEELERLE EACDMALELN ASKHRIYEYV ESRMSFIAPN LSIIIGASTA AKIMGVAGGL TN LSKMPAC NIMLLGAQRK TLSGFSSTSV LPHTGYIYHS DIVQSLPPDL RRKAARLVAA KCTLAARVDS FHESTEGKVG YEL KDEIER KFDKWQEPPP VKQVKPLPAP LDGQRKKRGG RRYRKMKERL GLTEIRKQAN RMSFGEIEED AYQEDLGFSL GHLG KSGSG RVRQTQVNEA TKARISKTLQ RTLQKQSVVY GGKSTIRDRS SGTASSVAFT PLQGLEIVNP QAAEKKVAEA NQKYF SSMA EFLKVKGEKS GLMST

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Macromolecule #11: Small nuclear ribonucleoprotein Sm D1

MacromoleculeName: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 11 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 13.310653 KDa
SequenceString:
MKLVRFLMKL SHETVTIELK NGTQVHGTIT GVDVSMNTHL KAVKMTLKNR EPVQLETLSI RGNNIRYFIL PDSLPLDTLL VDVEPKVKS KKREAVAGRG RGRGRGRGRG RGRGRGGPRR

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Macromolecule #12: RNA-binding protein 42

MacromoleculeName: RNA-binding protein 42 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 50.477922 KDa
SequenceString: MAGAGPAPGL PGAGGPVVPG PGAGIPGKSG EERLKEMEAE MALFEQEVLG APVPGIPTAV PAVPTVPTVP TVEAMQVPAA PVIRPIIAT NTYQQVQQTL EARAAAAATV VPPMVGGPPF VGPVGFGPGD RSHLDSPEAR EAMFLRRAAV APQRAPILRP A FVPHVLQR ...String:
MAGAGPAPGL PGAGGPVVPG PGAGIPGKSG EERLKEMEAE MALFEQEVLG APVPGIPTAV PAVPTVPTVP TVEAMQVPAA PVIRPIIAT NTYQQVQQTL EARAAAAATV VPPMVGGPPF VGPVGFGPGD RSHLDSPEAR EAMFLRRAAV APQRAPILRP A FVPHVLQR ADSALSSAAA GPRPMALRPP HQALVGPPLP GPPGPPMMLP PMARAPGPPL GSMAALRPPL EEPAAPRELG LG LGLGLKE KEEAVVAAAA GLEEASAAVA VGAGGAPAGP AVIGPSLPLA LAMPLPEPEP LPLPLEVVRG LLPPLRIPEL LSL RPRPRP PRPEPPPGLM ALEVPEPLGE DKKKGKPEKL KRCIRTAAGS SWEDPSLLEW DADDFRIFCG DLGNEVNDDI LARA FSRFP SFLKAKVIRD KRTGKTKGYG FVSFKDPSDY VRAMREMNGK YVGSRPIKLR KSMWKDRNLD VVRKKQKEKK KLGLR

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Macromolecule #13: Small nuclear ribonucleoprotein F

MacromoleculeName: Small nuclear ribonucleoprotein F / type: protein_or_peptide / ID: 13 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 9.734171 KDa
SequenceString:
MSLPLNPKPF LNGLTGKPVM VKLKWGMEYK GYLVSVDGYM NMQLANTEEY IDGALSGHLG EVLIRCNNVL YIRGVEEEEE DGEMRE

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Macromolecule #14: U6 snRNA-associated Sm-like protein LSm6

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm6 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 9.139571 KDa
SequenceString:
MSLRKQTPSD FLKQIIGRPV VVKLNSGVDY RGVLACLDGY MNIALEQTEE YVNGQLKNKY GDAFIRGNNV LYISTQKRRM

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Macromolecule #15: U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein

MacromoleculeName: U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein
type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 18.915414 KDa
SequenceString:
MGRSRSRSPR RERRRSRSTS RERERRRRER SRSRERDRRR SRSRSPHRRR SRSPRRHRST SPSPSRLKER RDEEKKETKE TKSKERQIT EEDLEGKTEE EIEMMKLMGF ASFDSTKGKK VDGSVNAYAI NVSQKRKYRQ YMNRKGGFNR PLDFIA

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Macromolecule #16: Small nuclear ribonucleoprotein Sm D2

MacromoleculeName: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 16 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 13.551928 KDa
SequenceString:
MSLLNKPKSE MTPEELQKRE EEEFNTGPLS VLTQSVKNNT QVLINCRNNK KLLGRVKAFD RHCNMVLENV KEMWTEVPKS GKGKKKSKP VNKDRYISKM FLRGDSVIVV LRNPLIAGK

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Macromolecule #18: U6 snRNA-associated Sm-like protein LSm7

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm7 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 11.617363 KDa
SequenceString:
MADKEKKKKE SILDLSKYID KTIRVKFQGG REASGILKGF DPLLNLVLDG TIEYMRDPDD QYKLTEDTRQ LGLVVCRGTS VVLICPQDG MEAIPNPFIQ QQDA

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Macromolecule #19: U6 snRNA-associated Sm-like protein LSm2

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm2 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 10.847495 KDa
SequenceString:
MLFYSFFKSL VGKDVVVELK NDLSICGTLH SVDQYLNIKL TDISVTDPEK YPHMLSVKNC FIRGSVVRYV QLPADEVDTQ LLQDAARKE ALQQKQ

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Macromolecule #20: U2 small nuclear ribonucleoprotein B''

MacromoleculeName: U2 small nuclear ribonucleoprotein B'' / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 25.524367 KDa
SequenceString: MDIRPNHTIY INNMNDKIKK EELKRSLYAL FSQFGHVVDI VALKTMKMRG QAFVIFKELG SSTNALRQLQ GFPFYGKPMR IQYAKTDSD IISKMRGTFA DKEKKKEKKK AKTVEQTATT TNKKPGQGTP NSANTQGNST PNPQVPDYPP NYILFLNNLP E ETNEMMLS ...String:
MDIRPNHTIY INNMNDKIKK EELKRSLYAL FSQFGHVVDI VALKTMKMRG QAFVIFKELG SSTNALRQLQ GFPFYGKPMR IQYAKTDSD IISKMRGTFA DKEKKKEKKK AKTVEQTATT TNKKPGQGTP NSANTQGNST PNPQVPDYPP NYILFLNNLP E ETNEMMLS MLFNQFPGFK EVRLVPGRHD IAFVEFENDG QAGAARDALQ GFKITPSHAM KITYAKK

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Macromolecule #21: Splicing factor 3A subunit 2

MacromoleculeName: Splicing factor 3A subunit 2 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 24.340717 KDa
SequenceString: MDFQHRPGGK TGSGGVASSS ESNRDRRERL RQLALETIDI NKDPYFMKNH LGSYECKLCL TLHNNEGSYL AHTQGKKHQT NLARRAAKE AKEAPAQPAP EKVKVEVKKF VKIGRPGYKV TKQRDSEMGQ QSLLFQIDYP EIAEGIMPRH RFMSAYEQRI E PPDRRWQY ...String:
MDFQHRPGGK TGSGGVASSS ESNRDRRERL RQLALETIDI NKDPYFMKNH LGSYECKLCL TLHNNEGSYL AHTQGKKHQT NLARRAAKE AKEAPAQPAP EKVKVEVKKF VKIGRPGYKV TKQRDSEMGQ QSLLFQIDYP EIAEGIMPRH RFMSAYEQRI E PPDRRWQY LLMAAEPYET IAFKVPSREI DKAEGKFWTH WNRETKQFFL Q

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Macromolecule #22: Splicing factor 3B subunit 2

MacromoleculeName: Splicing factor 3B subunit 2 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 100.377812 KDa
SequenceString: MATEHPEPPK AELQLPPPPP PGHYGAWAAQ ELQAKLAEIG APIQGNREEL VERLQSYTRQ TGIVLNRPVL RGEDGDKAAP PPMSAQLPG IPMPPPPLGL PPLQPPPPPP PPPPGLGLGF PMAHPPNLGP PPPLRVGEPV ALSEEERLKL AQQQAALLMQ Q EERAKQQG ...String:
MATEHPEPPK AELQLPPPPP PGHYGAWAAQ ELQAKLAEIG APIQGNREEL VERLQSYTRQ TGIVLNRPVL RGEDGDKAAP PPMSAQLPG IPMPPPPLGL PPLQPPPPPP PPPPGLGLGF PMAHPPNLGP PPPLRVGEPV ALSEEERLKL AQQQAALLMQ Q EERAKQQG DHSLKEHELL EQQKRAAVLL EQERQQEIAK MGTPVPRPPQ DMGQIGVRTP LGPRVAAPVG PVGPTPTVLP MG APVPRPR GPPPPPGDEN REMDDPSVGP KIPQALEKIL QLKESRQEEM NSQQEEEEME TDARSSLGQS ASETEEDTVS VSK KEKNRK RRNRKKKKKP QRVRGVSSES SGDREKDSTR SRGSDSPAAD VEIEYVTEEP EIYEPNFIFF KRIFEAFKLT DDVK KEKEK EPEKLDKLEN SAAPKKKGFE EEHKDSDDDS SDDEQEKKPE APKLSKKKLR RMNRFTVAEL KQLVARPDVV EMHDV TAQD PKLLVHLKAT RNSVPVPRHW CFKRKYLQGK RGIEKPPFEL PDFIKRTGIQ EMREALQEKE EQKTMKSKMR EKVRPK MGK IDIDYQKLHD AFFKWQTKPK LTIHGDLYYE GKEFETRLKE KKPGDLSDEL RISLGMPVGP NAHKVPPPWL IAMQRYG PP PSYPNLKIPG LNSPIPESCS FGYHAGGWGK PPVDETGKPL YGDVFGTNAA EFQTKTEEEE IDRTPWGELE PSDEESSE E EEEEESDEDK PDETGFITPA DSGLITPGGF SSVPAGMETP ELIELRKKKI EEAMDGSETP QLFTVLPEKR TATVGGAMM GSTHIYDMST VMSRKGPAPE LQGVEVALAP EELELDPMAM TQKYEEHVRE QQAQVEKEDF SDMVAEHAAK QKQKKRKAQP QDSRGGSKK YKEFKF

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Macromolecule #23: 116 kDa U5 small nuclear ribonucleoprotein component

MacromoleculeName: 116 kDa U5 small nuclear ribonucleoprotein component / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 95.968031 KDa
SequenceString: LMEQTLPVTV YEMDFLADLM DNSELIRNVT LCGHLHHGKT CFVDCLIEQT HPEIRKRYDQ DLCYTDILFT EQERGVGIKS TPVTVVLPD TKGKSYLFNI MDTPGHVNFS DEVTAGLRIS DGVVLFIDAA EGVMLNTERL IKHAVQERLA VTVCINKIDR L ILELKLPP ...String:
LMEQTLPVTV YEMDFLADLM DNSELIRNVT LCGHLHHGKT CFVDCLIEQT HPEIRKRYDQ DLCYTDILFT EQERGVGIKS TPVTVVLPD TKGKSYLFNI MDTPGHVNFS DEVTAGLRIS DGVVLFIDAA EGVMLNTERL IKHAVQERLA VTVCINKIDR L ILELKLPP TDAYYKLRHI VDEVNGLISM YSTDENLILS PLLGNVCFSS SQYSICFTLG SFAKIYADTF GDINYQEFAK RL WGDIYFN PKTRKFTKKA PTSSSQRSFV EFILEPLYKI LAQVVGDVDT SLPRTLDELG IHLTKEELKL NIRPLLRLVC KKF FGEFTG FVDMCVQHIP SPKVGAKPKI EHTYTGGVDS DLGEAMSDCD PDGPLMCHTT KMYSTDDGVQ FHAFGRVLSG TIHA GQPVK VLGENYTLED EEDSQICTVG RLWISVARYH IEVNRVPAGN WVLIEGVDQP IVKTATITEP RGNEEAQIFR PLKFN TTSV IKIAVEPVNP SELPKMLDGL RKVNKSYPSL TTKVEESGEH VILGTGELYL DCVMHDLRKM YSEIDIKVAD PVVTFC ETV VETSSLKCFA ETPNKKNKIT MIAEPLEKGL AEDIENEVVQ ITWNRKKLGE FFQTKYDWDL LAARSIWAFG PDATGPN IL VDDTLPSEVD KALLGSVKDS IVQGFQWGTR EGPLCDELIR NVKFKILDAV VAQEPLHRGG GQIIPTARRV VYSAFLMA T PRLMEPYYFV EVQAPADCVS AVYTVLARRR GHVTQDAPIP GSPLYTIKAF IPAIDSFGFE TDLRTHTQGQ AFSLSVFHH WQIVPGDPLD KSIVIRPLEP QPAPHLAREF MIKTRRRKGL SEDVSISKFF DDPM

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Macromolecule #24: Probable ATP-dependent RNA helicase DDX23

MacromoleculeName: Probable ATP-dependent RNA helicase DDX23 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 95.785148 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MAGELADKKD RDASPSKEER KRSRTPDRER DRDRDRKSSP SKDRKRHRSR DRRRGGSRSR SRSRSKSAER ERRHKERERD KERDRNKKD RDRDKDGHRR DKDRKRSSLS PGRGKDFKSR KDRDSKKDEE DEHGDKKPKA QPLSLEELLA KKKAEEEAEA K PKFLSKAE ...String:
MAGELADKKD RDASPSKEER KRSRTPDRER DRDRDRKSSP SKDRKRHRSR DRRRGGSRSR SRSRSKSAER ERRHKERERD KERDRNKKD RDRDKDGHRR DKDRKRSSLS PGRGKDFKSR KDRDSKKDEE DEHGDKKPKA QPLSLEELLA KKKAEEEAEA K PKFLSKAE REAEALKRRQ QEVEERQRML EEERKKRKQF QDLGRKMLED PQERERRERR ERMERETNGN EDEEGRQKIR EE KDKSKEL HAIKERYLGG IKKRRRTRHL NDRKFVFEWD ASEDTSIDYN PLYKERHQVQ LLGRGFIAGI DLKQQKREQS RFY GDLMEK RRTLEEKEQE EARLRKLRKK EAKQRWDDRH WSQKKLDEMT DRDWRIFRED YSITTKGGKI PNPIRSWKDS SLPP HILEV IDKCGYKEPT PIQRQAIPIG LQNRDIIGVA ETGSGKTAAF LIPLLVWITT LPKIDRIEES DQGPYAIILA PTREL AQQI EEETIKFGKP LGIRTVAVIG GISREDQGFR LRMGCEIVIA TPGRLIDVLE NRYLVLSRCT YVVLDEADRM IDMGFE PDV QKILEHMPVS NQKPDTDEAE DPEKMLANFE SGKHKYRQTV MFTATMPPAV ERLARSYLRR PAVVYIGSAG KPHERVE QK VFLMSESEKR KKLLAILEQG FDPPIIIFVN QKKGCDVLAK SLEKMGYNAC TLHGGKGQEQ REFALSNLKA GAKDILVA T DVAGRGIDIQ DVSMVVNYDM AKNIEDYIHR IGRTGRAGKS GVAITFLTKE DSAVFYELKQ AILESPVSSC PPELANHPD AQHKPGTILT KKRREETIFA

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Macromolecule #25: Small nuclear ribonucleoprotein-associated proteins B and B'

MacromoleculeName: Small nuclear ribonucleoprotein-associated proteins B and B'
type: protein_or_peptide / ID: 25 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 24.642131 KDa
SequenceString: MTVGKSSKML QHIDYRMRCI LQDGRIFIGT FKAFDKHMNL ILCDCDEFRK IKPKNSKQAE REEKRVLGLV LLRGENLVSM TVEGPPPKD TGIARVPLAG AAGGPGIGRA AGRGIPAGVP MPQAPAGLAG PVRGVGGPSQ QVMTPQGRGT VAAAAAAATA S IAGAPTQY ...String:
MTVGKSSKML QHIDYRMRCI LQDGRIFIGT FKAFDKHMNL ILCDCDEFRK IKPKNSKQAE REEKRVLGLV LLRGENLVSM TVEGPPPKD TGIARVPLAG AAGGPGIGRA AGRGIPAGVP MPQAPAGLAG PVRGVGGPSQ QVMTPQGRGT VAAAAAAATA S IAGAPTQY PPGRGGPPPP MGRGAPPPGM MGPPPGMRPP MGPPMGIPPG RGTPMGMPPP GMRPPPPGMR GPPPPGMRPP RP

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Macromolecule #26: Splicing factor 3B subunit 5

MacromoleculeName: Splicing factor 3B subunit 5 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 10.149369 KDa
SequenceString:
MTDRYTIHSQ LEHLQSKYIG TGHADTTKWE WLVNQHRDSY CSYMGHFDLL NYFAIAENES KARVRFNLME KMLQPCGPPA DKPEEN

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Macromolecule #27: U1 small nuclear ribonucleoprotein A

MacromoleculeName: U1 small nuclear ribonucleoprotein A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 31.319533 KDa
SequenceString: MAVPETRPNH TIYINNLNEK IKKDELKKSL YAIFSQFGQI LDILVSRSLK MRGQAFVIFK EVSSATNALR SMQGFPFYDK PMRIQYAKT DSDIIAKMKG TFVERDRKRE KRKPKSQETP ATKKAVQGGG ATPVVGAVQG PVPGMPPMTQ APRIMHHMPG Q PPYMPPPG ...String:
MAVPETRPNH TIYINNLNEK IKKDELKKSL YAIFSQFGQI LDILVSRSLK MRGQAFVIFK EVSSATNALR SMQGFPFYDK PMRIQYAKT DSDIIAKMKG TFVERDRKRE KRKPKSQETP ATKKAVQGGG ATPVVGAVQG PVPGMPPMTQ APRIMHHMPG Q PPYMPPPG MIPPPGLAPG QIPPGAMPPQ QLMPGQMPPA QPLSENPPNH ILFLTNLPEE TNELMLSMLF NQFPGFKEVR LV PGRHDIA FVEFDNEVQA GAARDALQGF KITQNNAMKI SFAKK

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Macromolecule #28: U4/U6.U5 tri-snRNP-associated protein 1

MacromoleculeName: U4/U6.U5 tri-snRNP-associated protein 1 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 90.414117 KDa
SequenceString: MGSSKKHRGE KEAAGTTAAA GTGGATEQPP RHREHKKHKH RSGGSGGSGG ERRKRSRERG GERGSGRRGA EAEARSSTHG RERSQAEPS ERRVKREKRD DGYEAAASSK TSSGDASSLS IEETNKLRAK LGLKPLEVNA IKKEAGTKEE PVTADVINPM A LRQREELR ...String:
MGSSKKHRGE KEAAGTTAAA GTGGATEQPP RHREHKKHKH RSGGSGGSGG ERRKRSRERG GERGSGRRGA EAEARSSTHG RERSQAEPS ERRVKREKRD DGYEAAASSK TSSGDASSLS IEETNKLRAK LGLKPLEVNA IKKEAGTKEE PVTADVINPM A LRQREELR EKLAAAKEKR LLNQKLGKIK TLGEDDPWLD DTAAWIERSR QLQKEKDLAE KRAKLLEEMD QEFGVSTLVE EE FGQRRQD LYSARDLQGL TVEHAIDSFR EGETMILTLK DKGVLQEEED VLVNVNLVDK ERAEKNVELR KKKPDYLPYA EDE SVDDLA QQKPRSILSK YDEELEGERP HSFRLEQGGT ADGLRERELE EIRAKLRLQA QSLSTVGPRL ASEYLTPEEM VTFK KTKRR VKKIRKKEKE VVVRADDLLP LGDQTQDGDF GSRLRGRGRR RVSEVEEEKE PVPQPLPSDD TRVENMDISD EEEGG APPP GSPQVLEEDE AELELQKQLE KGRRLRQLQQ LQQLRDSGEK VVEIVKKLES RQRGWEEDED PERKGAIVFN ATSEFC RTL GEIPTYGLAG NREEQEELMD FERDEERSAN GGSESDGEEN IGWSTVNLDE EKQQQDFSAS STTILDEEPI VNRGLAA AL LLCQNKGLLE TTVQKVARVK APNKSLPSAV YCIEDKMAID DKYSRREEYR GFTQDFKEKD GYKPDVKIEY VDETGRKL T PKEAFRQLSH RFHGKGSGKM KTERRMKKLD EEALLKKMSS SDTPLGTVAL LQEKQKAQKT PYIVLSGSGK SMNANTITK

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Macromolecule #29: Pre-mRNA-processing factor 6

MacromoleculeName: Pre-mRNA-processing factor 6 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 97.004688 KDa
SequenceString: VPGLGRGATG FTTRSDIGPA RDANDPVDDR HAPPGKRTVG DQMKKNQAAD DDDEDLNDTN YDEFNGYAGS LFSSGPYEKD DEEADAIYA ALDKRMDERR KERREQREKE EIEKYRMERP KIQQQFSDLK RKLAEVTEEE WLSIPEVGDG ELDMRKIGQA R NTLMDMRL ...String:
VPGLGRGATG FTTRSDIGPA RDANDPVDDR HAPPGKRTVG DQMKKNQAAD DDDEDLNDTN YDEFNGYAGS LFSSGPYEKD DEEADAIYA ALDKRMDERR KERREQREKE EIEKYRMERP KIQQQFSDLK RKLAEVTEEE WLSIPEVGDG ELDMRKIGQA R NTLMDMRL SQVSDSVSGQ TVVDPKGYLT DLNSMIPTHG GDINDIKKAR LLLKSVRETN PHHPPAWIAS ARLEEVTGKL QV ARNLIMK GTEMCPKSED VWLEAARLQP GDTAKAVVAQ AVRHLPQSVR IYIRAAELET DIRAKKRVLR KALEHVPNSV RLW KAAVEL EEPEDARIML SRAVECCPTS VELWLALARL ETYENARKVL NKARENIPTD RHIWITAAKL EEANGNTQMV EKII DRAIT SLRANGVEIN REQWIQDAEE CDRAGSVATC QAVMRAVIGI GIEEEDRKHT WMEDADSCVA HNALECARAI YAYAL QVFP SKKSVWLRAA YFEKNHGTRE SLEALLQRAV AHCPKAEVLW LMGAKSKWLA GDVPAARSIL ALAFQANPNS EEIWLA AVK LESENDEYER ARRLLAKARS SAPTARVFMK SVKLEWVQDN IRAAQDLCEE ALRHYEDFPK LWMMKGQIEE QKEMMEK AR EAYNQGLKKC PHSTPLWLLL SRLEEKIGQL TRARAILEKS RLKNPKNPGL WLESVRLEYR AGLKNIANTL MAKALQEC P NSGILWSEAI FLEARPQRRT KSVDALKKCE HDPHVLLAVA KLFWSQRKIT KAREWFHRTV KIDSDLGDAW AFFYKFELQ HGTEEQQEEV RKRCESAEPR HGELWCAVSK DIANWQKKIG DILRLVAGRI

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Macromolecule #30: NHP2-like protein 1

MacromoleculeName: NHP2-like protein 1 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 14.191524 KDa
SequenceString:
MTEADVNPKA YPLADAHLTK KLLDLVQQSC NYKQLRKGAN EATKTLNRGI SEFIVMAADA EPLEIILHLP LLCEDKNVPY VFVRSKQAL GRACGVSRPV IACSVTIKEG SQLKQQIQSI QQSIERLLV

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Macromolecule #31: U6 snRNA-associated Sm-like protein LSm3

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm3 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 11.85939 KDa
SequenceString:
MADDVDQQQT TNTVEEPLDL IRLSLDERIY VKMRNDRELR GRLHAYDQHL NMILGDVEET VTTIEIDEET YEEIYKSTKR NIPMLFVRG DGVVLVAPPL RVG

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Macromolecule #33: Splicing factor 3B subunit 3

MacromoleculeName: Splicing factor 3B subunit 3 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 135.718844 KDa
SequenceString: MFLYNLTLQR ATGISFAIHG NFSGTKQQEI VVSRGKILEL LRPDPNTGKV HTLLTVEVFG VIRSLMAFRL TGGTKDYIVV GSDSGRIVI LEYQPSKNMF EKIHQETFGK SGCRRIVPGQ FLAVDPKGRA VMISAIEKQK LVYILNRDAA ARLTISSPLE A HKANTLVY ...String:
MFLYNLTLQR ATGISFAIHG NFSGTKQQEI VVSRGKILEL LRPDPNTGKV HTLLTVEVFG VIRSLMAFRL TGGTKDYIVV GSDSGRIVI LEYQPSKNMF EKIHQETFGK SGCRRIVPGQ FLAVDPKGRA VMISAIEKQK LVYILNRDAA ARLTISSPLE A HKANTLVY HVVGVDVGFE NPMFACLEMD YEEADNDPTG EAAANTQQTL TFYELDLGLN HVVRKYSEPL EEHGNFLITV PG GSDGPSG VLICSENYIT YKNFGDQPDI RCPIPRRRND LDDPERGMIF VCSATHKTKS MFFFLAQTEQ GDIFKITLET DED MVTEIR LKYFDTVPVA AAMCVLKTGF LFVASEFGNH YLYQIAHLGD DDEEPEFSSA MPLEEGDTFF FQPRPLKNLV LVDE LDSLS PILFCQIADL ANEDTPQLYV ACGRGPRSSL RVLRHGLEVS EMAVSELPGN PNAVWTVRRH IEDEFDAYII VSFVN ATLV LSIGETVEEV TDSGFLGTTP TLSCSLLGDD ALVQVYPDGI RHIRADKRVN EWKTPGKKTI VKCAVNQRQV VIALTG GEL VYFEMDPSGQ LNEYTERKEM SADVVCMSLA NVPPGEQRSR FLAVGLVDNT VRIISLDPSD CLQPLSMQAL PAQPESL CI VEMGGTEKQD ELGERGSIGF LYLNIGLQNG VLLRTVLDPV TGDLSDTRTR YLGSRPVKLF RVRMQGQEAV LAMSSRSW L SYSYQSRFHL TPLSYETLEF ASGFASEQCP EGIVAISTNT LRILALEKLG AVFNQVAFPL QYTPRKFVIH PESNNLIII ETDHNAYTEA TKAQRKQQMA EEMVEAAGED ERELAAEMAA AFLNENLPES IFGAPKAGNG QWASVIRVMN PIQGNTLDLV QLEQNEAAF SVAVCRFSNT GEDWYVLVGV AKDLILNPRS VAGGFVYTYK LVNNGEKLEF LHKTPVEEVP AAIAPFQGRV L IGVGKLLR VYDLGKKKLL RKCENKHIAN YISGIQTIGH RVIVSDVQES FIWVRYKRNE NQLIIFADDT YPRWVTTASL LD YDTVAGA DKFGNICVVR LPPNTNDEVD EDPTGNKALW DRGLLNGASQ KAEVIMNYHV GETVLSLQKT TLIPGGSESL VYT TLSGGI GILVPFTSHE DHDFFQHVEM HLRSEHPPLC GRDHLSFRSY YFPVKNVIDG DLCEQFNSME PNKQKNVSEE LDRT PPEVS KKLEDIRTRY AF

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Macromolecule #34: Small nuclear ribonucleoprotein G

MacromoleculeName: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 34 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 8.508084 KDa
SequenceString:
MSKAHPPELK KFMDKKLSLK LNGGRHVQGI LRGFDPFMNL VIDECVEMAT SGQQNNIGMV VIRGNSIIML EALERV

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Macromolecule #35: U6 snRNA-associated Sm-like protein LSm8

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm8 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 10.410589 KDa
SequenceString:
MTSALENYIN RTVAVITSDG RMIVGTLKGF DQTINLILDE SHERVFSSSQ GVEQVVLGLY IVRGDNVAVI GEIDEETDSA LDLGNIRAE PLNSVAH

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Macromolecule #36: Pre-mRNA-processing-splicing factor 8

MacromoleculeName: Pre-mRNA-processing-splicing factor 8 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 271.508281 KDa
SequenceString: MSEEKLQEKA RKWQQLQAKR YAEKRKFGFV DAQKEDMPPE HVRKIIRDHG DMTNRKFRHD KRVYLGALKY MPHAVLKLLE NMPMPWEQI RDVPVLYHIT GAISFVNEIP WVIEPVYISQ WGSMWIMMRR EKRDRRHFKR MRFPPFDDEE PPLDYADNIL D VEPLEAIQ ...String:
MSEEKLQEKA RKWQQLQAKR YAEKRKFGFV DAQKEDMPPE HVRKIIRDHG DMTNRKFRHD KRVYLGALKY MPHAVLKLLE NMPMPWEQI RDVPVLYHIT GAISFVNEIP WVIEPVYISQ WGSMWIMMRR EKRDRRHFKR MRFPPFDDEE PPLDYADNIL D VEPLEAIQ LELDPEEDAP VLDWFYDHQP LRDSRKYVNG STYQRWQFTL PMMSTLYRLA NQLLTDLVDD NYFYLFDLKA FF TSKALNM AIPGGPKFEP LVRDINLQDE DWNEFNDINK IIIRQPIRTE YKIAFPYLYN NLPHHVHLTW YHTPNVVFIK TED PDLPAF YFDPLINPIS HRHSVKSQEP LPDDDEEFEL PEFVEPFLKD TPLYTDNTAN GIALLWAPRP FNLRSGRTRR ALDI PLVKN WYREHCPAGQ PVKVRVSYQK LLKYYVLNAL KHRPPKAQKK RYLFRSFKAT KFFQSTKLDW VEVGLQVCRQ GYNML NLLI HRKNLNYLHL DYNFNLKPVK TLTTKERKKS RFGNAFHLCR EVLRLTKLVV DSHVQYRLGN VDAFQLADGL QYIFAH VGQ LTGMYRYKYK LMRQIRMCKD LKHLIYYRFN TGPVGKGPGC GFWAAGWRVW LFFMRGITPL LERWLGNLLA RQFEGRH SK GVAKTVTKQR VESHFDLELR AAVMHDILDM MPEGIKQNKA RTILQHLSEA WRCWKANIPW KVPGLPTPIE NMILRYVK A KADWWTNTAH YNRERIRRGA TVDKTVCKKN LGRLTRLYLK AEQERQHNYL KDGPYITAEE AVAVYTTTVH WLESRRFSP IPFPPLSYKH DTKLLILALE RLKEAYSVKS RLNQSQREEL GLIEQAYDNP HEALSRIKRH LLTQRAFKEV GIEFMDLYSH LVPVYDVEP LEKITDAYLD QYLWYEADKR RLFPPWIKPA DTEPPPLLVY KWCQGINNLQ DVWETSEGEC NVMLESRFEK M YEKIDLTL LNRLLRLIVD HNIADYMTAK NNVVINYKDM NHTNSYGIIR GLQFASFIVQ YYGLVMDLLV LGLHRASEMA GP PQMPNDF LSFQDIATEA AHPIRLFCRY IDRIHIFFRF TADEARDLIQ RYLTEHPDPN NENIVGYNNK KCWPRDARMR LMK HDVNLG RAVFWDIKNR LPRSVTTVQW ENSFVSVYSK DNPNLLFNMC GFECRILPKC RTSYEEFTHK DGVWNLQNEV TKER TAQCF LRVDDESMQR FHNRVRQILM ASGSTTFTKI VNKWNTALIG LMTYFREAVV NTQELLDLLV KCENKIQTRI KIGLN SKMP SRFPPVVFYT PKELGGLGML SMGHVLIPQS DLRWSKQTDV GITHFRSGMS HEEDQLIPNL YRYIQPWESE FIDSQR VWA EYALKRQEAI AQNRRLTLED LEDSWDRGIP RINTLFQKDR HTLAYDKGWR VRTDFKQYQV LKQNPFWWTH QRHDGKL WN LNNYRTDMIQ ALGGVEGILE HTLFKGTYFP TWEGLFWEKA SGFEESMKWK KLTNAQRSGL NQIPNRRFTL WWSPTINR A NVYVGFQVQL DLTGIFMHGK IPTLKISLIQ IFRAHLWQKI HESIVMDLCQ VFDQELDALE IETVQKETIH PRKSYKMNS SCADILLFAS YKWNVSRPSL LADSKDVMDS TTTQKYWIDI QLRWGDYDSH DIERYARAKF LDYTTDNMSI YPSPTGVLIA IDLAYNLHS AYGNWFPGSK PLIQQAMAKI MKANPALYVL RERIRKGLQL YSSEPTEPYL SSQNYGELFS NQIIWFVDDT N VYRVTIHK TFEGNLTTKP INGAIFIFNP RTGQLFLKII HTSVWAGQKR LGQLAKWKTA EEVAALIRSL PVEEQPKQII VT RKGMLDP LEVHLLDFPN IVIKGSELQL PFQACLKVEK FGDLILKATE PQMVLFNLYD DWLKTISSYT AFSRLILILR ALH VNNDRA KVILKPDKTT ITEPHHIWPT LTDEEWIKVE VQLKDLILAD YGKKNNVNVA SLTQSEIRDI ILGMEISAPS QQRQ QIAEI EKQTKEQSQL TATQTRTVNK HGDEIITSTT SNYETQTFSS KTEWRVRAIS AANLHLRTNH IYVSSDDIKE TGYTY ILPK NVLKKFICIS DLRAQIAGYL YGVSPPDNPQ VKEIRCIVMV PQWGTHQTVH LPGQLPQHEY LKEMEPLGWI HTQPNE SPQ LSPQDVTTHA KIMADNPSWD GEKTIIITCS FTPGSCTLTA YKLTPSGYEW GRQNTDKGNN PKGYLPSHYE RVQMLLS DR FLGFFMVPAQ SSWNYNFMGV RHDPNMKYEL QLANPKEFYH EVHRPSHFLN FALLQEGEVY SADREDLYA

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Macromolecule #37: Splicing factor 3A subunit 3

MacromoleculeName: Splicing factor 3A subunit 3 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 58.934844 KDa
SequenceString: METILEQQRR YHEEKERLMD VMAKEMLTKK STLRDQINSD HRTRAMQDRY MEVSGNLRDL YDDKDGLRKE ELNAISGPNE FAEFYNRLK QIKEFHRKHP NEICVPMSVE FEELLKAREN PSEEAQNLVE FTDEEGYGRY LDLHDCYLKY INLKASEKLD Y ITYLSIFD ...String:
METILEQQRR YHEEKERLMD VMAKEMLTKK STLRDQINSD HRTRAMQDRY MEVSGNLRDL YDDKDGLRKE ELNAISGPNE FAEFYNRLK QIKEFHRKHP NEICVPMSVE FEELLKAREN PSEEAQNLVE FTDEEGYGRY LDLHDCYLKY INLKASEKLD Y ITYLSIFD QLFDIPKERK NAEYKRYLEM LLEYLQDYTD RVKPLQDQNE LFGKIQAEFE KKWENGTFPG WPKETSSALT HA GAHLDLS AFSSWEELAS LGLDRLKSAL LALGLKCGGT LEERAQRLFS TKGKSLESLD TSLFAKNPKS KGTKRDTERN KDI AFLEAQ IYEYVEILGE QRHLTHENVQ RKQARTGEER EEEEEEQISE SESEDEENEI IYNPKNLPLG WDGKPIPYWL YKLH GLNIN YNCEICGNYT YRGPKAFQRH FAEWRHAHGM RCLGIPNTAH FANVTQIEDA VSLWAKLKLQ KASERWQPDT EEEYE DSSG NVVNKKTYED LKRQGLL

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Macromolecule #38: U4/U6.U5 tri-snRNP-associated protein 2

MacromoleculeName: U4/U6.U5 tri-snRNP-associated protein 2 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 64.408445 KDa
SequenceString: MSGRSKRESR GSTRGKRESE SRGSSGRVKR ERDREREPEA ASSRGSPVRV KREFEPASAR EAPASVVPFV RVKREREVDE DSEPEREVR AKNGRVDSED RRSRHCPYLD TINRSVLDFD FEKLCSISLS HINAYACLVC GKYFQGRGLK SHAYIHSVQF S HHVFLNLH ...String:
MSGRSKRESR GSTRGKRESE SRGSSGRVKR ERDREREPEA ASSRGSPVRV KREFEPASAR EAPASVVPFV RVKREREVDE DSEPEREVR AKNGRVDSED RRSRHCPYLD TINRSVLDFD FEKLCSISLS HINAYACLVC GKYFQGRGLK SHAYIHSVQF S HHVFLNLH TLKFYCLPDN YEIIDSSLED ITYVLKPTFT KQQIANLDKQ AKLSRAYDGT TYLPGIVGLN NIKANDYANA VL QALSNVP PLRNYFLEED NYKNIKRPPG DIMFLLVQRF GELMRKLWNP RNFKAHVSPH EMLQAVVLCS KKTFQITKQG DGV DFLSWF LNALHSALGG TKKKKKTIVT DVFQGSMRIF TKKLPHPDLP AEEKEQLLHN DEYQETMVES TFMYLTLDLP TAPL YKDEK EQLIIPQVPL FNILAKFNGI TEKEYKTYKE NFLKRFQLTK LPPYLIFCIK RFTKNNFFVE KNPTIVNFPI TNVDL REYL SEEVQAVHKN TTYDLIANIV HDGKPSEGSY RIHVLHHGTG KWYELQDLQV TDILPQMITL SEAYIQIWKR R

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Macromolecule #39: Thioredoxin-like protein 4A

MacromoleculeName: Thioredoxin-like protein 4A / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 16.807346 KDa
SequenceString:
MSYMLPHLHN GWQVDQAILS EEDRVVVIRF GHDWDPTCMK MDEVLYSIAE KVKNFAVIYL VDITEVPDFN KMYELYDPCT VMFFFRNKH IMIDLGTGNN NKINWAMEDK QEMVDIIETV YRGARKGRGL VVSPKDYSTK YRY

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Macromolecule #40: U6 snRNA-associated Sm-like protein LSm4

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm4 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 15.375775 KDa
SequenceString:
MLPLSLLKTA QNHPMLVELK NGETYNGHLV SCDNWMNINL REVICTSRDG DKFWRMPECY IRGSTIKYLR IPDEIIDMVK EEVVAKGRG RGGLQQQKQQ KGRGMGGAGR GVFGGRGRGG IPGTGRGQPE KKPGRQAGKQ

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Macromolecule #41: PHD finger-like domain-containing protein 5A

MacromoleculeName: PHD finger-like domain-containing protein 5A / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 11.64457 KDa
SequenceString:
MAKHHPDLIF CRKQAGVAIG RLCEKCDGKC VICDSYVRPC TLVRICDECN YGSYQGRCVI CGGPGVSDAY YCKECTIQEK DRDGCPKIV NLGSSKTDLF YERKK

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Macromolecule #42: U1 small nuclear ribonucleoprotein C

MacromoleculeName: U1 small nuclear ribonucleoprotein C / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 17.415727 KDa
SequenceString:
MPKFYCDYCD TYLTHDSPSV RKTHCSGRKH KENVKDYYQK WMEEQAQSLI DKTTAAFQQG KIPPTPFSAP PPAGAMIPPP PSLPGPPRP GMMPAPHMGG PPMMPMMGPP PPGMMPVGPA PGMRPPMGGH MPMMPGPPMM RPPARPMMVP TRPGMTRPDR

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Macromolecule #43: Serine/threonine-protein kinase PRP4 homolog

MacromoleculeName: Serine/threonine-protein kinase PRP4 homolog / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO / EC number: non-specific serine/threonine protein kinase
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 117.264977 KDa
SequenceString: MAAAETQSLR EQPEMEDANS EKSINEENGE VSEDQSQNKH SRHKKKKHKH RSKHKKHKHS SEEDKDKKHK HKHKHKKHKR KEIIDASDK EGMSPAKRTK LDDLALLEDL EKQRALIKAE LDNELMEGKV QSGMGLILQG YESGSEEEGE IHEKARNGNR S STRSSSTK ...String:
MAAAETQSLR EQPEMEDANS EKSINEENGE VSEDQSQNKH SRHKKKKHKH RSKHKKHKHS SEEDKDKKHK HKHKHKKHKR KEIIDASDK EGMSPAKRTK LDDLALLEDL EKQRALIKAE LDNELMEGKV QSGMGLILQG YESGSEEEGE IHEKARNGNR S STRSSSTK GKLELVDNKI TTKKRSKSRS KERTRHRSDK KKSKGGIEIV KEKTTRSKSK ERKKSKSPSK RSKSQDQARK SK SPTLRRR SQEKIGKARS PTDDKVKIED KSKSKDRKKS PIINESRSRD RGKKSRSPVD LRGKSKDRRS RSKERKSKRS ETD KEKKPI KSPSKDASSG KENRSPSRRP GRSPKRRSLS PKPRDKSRRS RSPLLNDRRS KQSKSPSRTL SPGRRAKSRS LERK RREPE RRRLSSPRTR PRDDILSRRE RSKDASPINR WSPTRRRSRS PIRRRSRSPL RRSRSPRRRS RSPRRRDRGR RSRSR LRRR SRSRGGRRRR SRSKVKEDKF KGSLSEGMKV EQESSSDDNL EDFDVEEEDE EALIEQRRIQ RQAIVQKYKY LAEDSN MSV PSEPSSPQSS TRTRSPSPDD ILERVAADVK EYERENVDTF EASVKAKHNL MTVEQNNGSS QKKLLAPDMF TESDDMF AA YFDSARLRAA GIGKDFKENP NLRDNWTDAE GYYRVNIGEV LDKRYNVYGY TGQGVFSNVV RARDNARANQ EVAVKIIR N NELMQKTGLK ELEFLKKLND ADPDDKFHCL RLFRHFYHKQ HLCLVFEPLS MNLREVLKKY GKDVGLHIKA VRSYSQQLF LALKLLKRCN ILHADIKPDN ILVNESKTIL KLCDFGSASH VADNDITPYL VSRFYRAPEI IIGKSYDYGI DMWSVGCTLY ELYTGKILF PGKTNNHMLK LAMDLKGKMP NKMIRKGVFK DQHFDQNLNF MYIEVDKVTE REKVTVMSTI NPTKDLLADL I GCQRLPED QRKKVHQLKD LLDQILMLDP AKRISINQAL QHAFIQEKI

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Macromolecule #44: U4/U6 small nuclear ribonucleoprotein Prp3

MacromoleculeName: U4/U6 small nuclear ribonucleoprotein Prp3 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 77.669188 KDa
SequenceString: MALSKRELDE LKPWIEKTVK RVLGFSEPTV VTAALNCVGK GMDKKKAADH LKPFLDDSTL RFVDKLFEAV EEGRSSRHSK SSSDRSRKR ELKEVFGDDS EISKESSGVK KRRIPRFEEV EEEPEVIPGP PSESPGMLTK LQIKQMMEAA TRQIEERKKQ L SFISPPTP ...String:
MALSKRELDE LKPWIEKTVK RVLGFSEPTV VTAALNCVGK GMDKKKAADH LKPFLDDSTL RFVDKLFEAV EEGRSSRHSK SSSDRSRKR ELKEVFGDDS EISKESSGVK KRRIPRFEEV EEEPEVIPGP PSESPGMLTK LQIKQMMEAA TRQIEERKKQ L SFISPPTP QPKTPSSSQP ERLPIGNTIQ PSQAATFMND AIEKARKAAE LQARIQAQLA LKPGLIGNAN MVGLANLHAM GI APPKVEL KDQTKPTPLI LDEQGRTVDA TGKEIELTHR MPTLKANIRA VKREQFKQQL KEKPSEDMES NTFFDPRVSI APS QRQRRT FKFHDKGKFE KIAQRLRTKA QLEKLQAEIS QAARKTGIHT STRLALIAPK KELKEGDIPE IEWWDSYIIP NGFD LTEEN PKREDYFGIT NLVEHPAQLN PPVDNDTPVT LGVYLTKKEQ KKLRRQTRRE AQKELQEKVR LGLMPPPEPK VRISN LMRV LGTEAVQDPT KVEAHVRAQM AKRQKAHEEA NAARKLTAEQ RKVKKIKKLK EDISQGVHIS VYRVRNLSNP AKKFKI EAN AGQLYLTGVV VLHKDVNVVV VEGGPKAQKK FKRLMLHRIK WDEQTSNTKG DDDEESDEEA VKKTNKCVLV WEGTAKD RS FGEMKFKQCP TENMAREHFK KHGAEHYWDL ALSESVLEST D

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Macromolecule #46: U2 small nuclear ribonucleoprotein A'

MacromoleculeName: U2 small nuclear ribonucleoprotein A' / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 28.456584 KDa
SequenceString: MVKLTAELIE QAAQYTNAVR DRELDLRGYK IPVIENLGAT LDQFDAIDFS DNEIRKLDGF PLLRRLKTLL VNNNRICRIG EGLDQALPC LTELILTNNS LVELGDLDPL ASLKSLTYLS ILRNPVTNKK HYRLYVIYKV PQVRVLDFQK VKLKERQEAE K MFKGKRGA ...String:
MVKLTAELIE QAAQYTNAVR DRELDLRGYK IPVIENLGAT LDQFDAIDFS DNEIRKLDGF PLLRRLKTLL VNNNRICRIG EGLDQALPC LTELILTNNS LVELGDLDPL ASLKSLTYLS ILRNPVTNKK HYRLYVIYKV PQVRVLDFQK VKLKERQEAE K MFKGKRGA QLAKDIARRS KTFNPGAGLP TDKKKGGPSP GDVEAIKNAI ANASTLAEVE RLKGLLQSGQ IPGRERRSGP TD DGEEEME EDTVTNGS

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Macromolecule #47: Splicing factor 3A subunit 1,Splicing factor 3A subunit 1,Splicin...

MacromoleculeName: Splicing factor 3A subunit 1,Splicing factor 3A subunit 1,Splicing factor 3A subunit 1
type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 71.619383 KDa
SequenceString: MPAGPVQAVP PPPPVPTEPK QPTEEEASSK EDSAPSKPVV GIIYPPPEVR NIVDKTASFV ARNGPEFEAR IRQNEINNPK FNFLNPNDP YHAYYRHKVS EFKEGKAQEP SAAIPKVMQQ QQQTTQQQLP QKVQAQVIQE TIVPKEPPPE FEFIADPPSI S AFDLDVVK ...String:
MPAGPVQAVP PPPPVPTEPK QPTEEEASSK EDSAPSKPVV GIIYPPPEVR NIVDKTASFV ARNGPEFEAR IRQNEINNPK FNFLNPNDP YHAYYRHKVS EFKEGKAQEP SAAIPKVMQQ QQQTTQQQLP QKVQAQVIQE TIVPKEPPPE FEFIADPPSI S AFDLDVVK LTAQFVARNG RQFLTQLMQK EQRNYQFDFL RPQHSLFNYF TKLVEQYTKI LIPPKGLFSK LKKEAENPRE VL DQVCYRV EWAKFQERER KKEEEEKEKE RVAYAQIDWH DFVVV(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)VYAPGLD IESSLKQLAE RRTDIFGVEE TAIGKKIGEE EIQKPEEKVT WDGHSGSMAR TQQAAQAN I TLQEQIEAIH KAKGLVPEDD TKEKIGPSKP NEIPQQPPPP SSATNIPSSA PPITSVPRPP TMPPPVRTTV VSAVPVMPR PPMASVVRLP PGSVIAPMPP IIHAPRINVV PMPPSAPPIM APRPPPMIVP TAFVPAPPVA PVPAPAPMPP VHPPPPMEDE PTSKKLKTE DSLMPEEEFL RRNKGPVSIK VQVPNMQDKT EWKLNGQVLV FTLPLTDQVS VIKVKIHEAT GMPAGKQKLQ Y EGIFIKDS NSLAYYNMAN GAVIHLALKE RGGRKK

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Macromolecule #48: U6 snRNA-associated Sm-like protein LSm5

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm5 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 9.945448 KDa
SequenceString:
MAANATTNPS QLLPLELVDK CIGSRIHIVM KSDKEIVGTL LGFDDFVNMV LEDVTEFEIT PEGRRITKLD QILLNGNNIT MLVPGGEGP EV

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Macromolecule #49: U5 small nuclear ribonucleoprotein 200 kDa helicase

MacromoleculeName: U5 small nuclear ribonucleoprotein 200 kDa helicase / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 244.823422 KDa
SequenceString: MADVTARSLQ YEYKANSNLV LQADRSLIDR TRRDEPTGEV LSLVGKLEGT RMGDKAQRTK PQMQEERRAK RRKRDEDRHD INKMKGYTL LSEGIDEMVG IIYKPKTKET RETYEVLLSF IQAALGDQPR DILCGAADEV LAVLKNEKLR DKERRKEIDL L LGQTDDTR ...String:
MADVTARSLQ YEYKANSNLV LQADRSLIDR TRRDEPTGEV LSLVGKLEGT RMGDKAQRTK PQMQEERRAK RRKRDEDRHD INKMKGYTL LSEGIDEMVG IIYKPKTKET RETYEVLLSF IQAALGDQPR DILCGAADEV LAVLKNEKLR DKERRKEIDL L LGQTDDTR YHVLVNLGKK ITDYGGDKEI QNMDDNIDET YGVNVQFESD EEEGDEDVYG EVREEASDDD MEGDEAVVRC TL SANLVAS GELMSSKKKD LHPRDIDAFW LQRQLSRFYD DAIVSQKKAD EVLEILKTAS DDRECENQLV LLLGFNTFDF IKV LRQHRM MILYCTLLAS AQSEAEKERI MGKMEADPEL SKFLYQLHET EKEDLIREER SRRERVRQSR MDTDLETMDL DQGG EALAP RQVLDLEDLV FTQGSHFMAN KRCQLPDGSF RRQRKGYEEV HVPALKPKPF GSEEQLLPVE KLPKYAQAGF EGFKT LNRI QSKLYRAALE TDENLLLCAP TGAGKTNVAL MCMLREIGKH INMDGTINVD DFKIIYIAPM RSLVQEMVGS FGKRLA TYG ITVAELTGDH QLCKEEISAT QIIVCTPEKW DIITRKGGER TYTQLVRLII LDEIHLLHDD RGPVLEALVA RAIRNIE MT QEDVRLIGLS ATLPNYEDVA TFLRVDPAKG LFYFDNSFRP VPLEQTYVGI TEKKAIKRFQ IMNEIVYEKI MEHAGKNQ V LVFVHSRKET GKTARAIRDM CLEKDTLGLF LREGSASTEV LRTEAEQCKN LELKDLLPYG FAIHHAGMTR VDRTLVEDL FADKHIQVLV STATLAWGVN LPAHTVIIKG TQVYSPEKGR WTELGALDIL QMLGRAGRPQ YDTKGEGILI TSHGELQYYL SLLNQQLPI ESQMVSKLPD MLNAEIVLGN VQNAKDAVNW LGYAYLYIRM LRSPTLYGIS HDDLKGDPLL DQRRLDLVHT A ALMLDKNN LVKYDKKTGN FQVTELGRIA SHYYITNDTV QTYNQLLKPT LSEIELFRVF SLSSEFKNIT VREEEKLELQ KL LERVPIP VKESIEEPSA KINVLLQAFI SQLKLEGFAL MADMVYVTQS AGRLMRAIFE IVLNRGWAQL TDKTLNLCKM IDK RMWQSM CPLRQFRKLP EEVVKKIEKK NFPFERLYDL NHNEIGELIR MPKMGKTIHK YVHLFPKLEL SVHLQPITRS TLKV ELTIT PDFQWDEKVH GSSEAFWILV EDVDSEVILH HEYFLLKAKY AQDEHLITFF VPVFEPLPPQ YFIRVVSDRW LSCET QLPV SFRHLILPEK YPPPTELLDL QPLPVSALRN SAFESLYQDK FPFFNPIQTQ VFNTVYNSDD NVFVGAPTGS GKTICA EFA ILRMLLQSSE GRCVYITPME ALAEQVYMDW YEKFQDRLNK KVVLLTGETS TDLKLLGKGN IIISTPEKWD ILSRRWK QR KNVQNINLFV VDEVHLIGGE NGPVLEVICS RMRYISSQIE RPIRIVALSS SLSNAKDVAH WLGCSATSTF NFHPNVRP V PLELHIQGFN ISHTQTRLLS MAKPVYHAIT KHSPKKPVIV FVPSRKQTRL TAIDILTTCA ADIQRQRFLH CTEKDLIPY LEKLSDSTLK ETLLNGVGYL HEGLSPMERR LVEQLFSSGA IQVVVASRSL CWGMNVAAHL VIIMDTQYYN GKIHAYVDYP IYDVLQMVG HANRPLQDDE GRCVIMCQGS KKDFFKKFLY EPLPVESHLD HCMHDHFNAE IVTKTIENKQ DAVDYLTWTF L YRRMTQNP NYYNLQGISH RHLSDHLSEL VEQTLSDLEQ SKCISIEDEM DVAPLNLGMI AAYYYINYTT IELFSMSLNA KT KVRGLIE IISNAAEYEN IPIRHHEDNL LRQLAQKVPH KLNNPKFNDP HVKTNLLLQA HLSRMQLSAE LQSDTEEILS KAI RLIQAC VDVLSSNGWL SPALAAMELA QMVTQAMWSK DSYLKQLPHF TSEHIKRCTD KGVESVFDIM EMEDEERNAL LQLT DSQIA DVARFCNRYP NIELSYEVVD KDSIRSGGPV VVLVQLEREE EVTGPVIAPL FPQKREEGWW VVIGDAKSNS LISIK RLTL QQKAKVKLDF VAPATGAHNY TLYFMSDAYM GCDQEYKFSV DVKEAETDSD SD

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Macromolecule #50: Splicing factor 3B subunit 1

MacromoleculeName: Splicing factor 3B subunit 1 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 146.024938 KDa
SequenceString: MAKIAKTHED IEAQIREIQG KKAALDEAQG VGLDSTGYYD QEIYGGSDSR FAGYVTSIAA TELEDDDDDY SSSTSLLGQK KPGYHAPVA LLNDIPQSTE QYDPFAEHRP PKIADREDEY KKHRRTMIIS PERLDPFADG GKTPDPKMNA RTYMDVMREQ H LTKEEREI ...String:
MAKIAKTHED IEAQIREIQG KKAALDEAQG VGLDSTGYYD QEIYGGSDSR FAGYVTSIAA TELEDDDDDY SSSTSLLGQK KPGYHAPVA LLNDIPQSTE QYDPFAEHRP PKIADREDEY KKHRRTMIIS PERLDPFADG GKTPDPKMNA RTYMDVMREQ H LTKEEREI RQQLAEKAKA GELKVVNGAA ASQPPSKRKR RWDQTADQTP GATPKKLSSW DQAETPGHTP SLRWDETPGR AK GSETPGA TPGSKIWDPT PSHTPAGAAT PGRGDTPGHA TPGHGGATSS ARKNRWDETP KTERDTPGHG SGWAETPRTD RGG DSIGET PTPGASKRKS RWDETPASQM GGSTPVLTPG KTPIGTPAMN MATPTPGHIM SMTPEQLQAW RWEREIDERN RPLS DEELD AMFPEGYKVL PPPAGYVPIR TPARKLTATP TPLGGMTGFH MQTEDRTMKS VNDQPSGNLP FLKPDDIQYF DKLLV DVDE STLSPEEQKE RKIMKLLLKI KNGTPPMRKA ALRQITDKAR EFGAGPLFNQ ILPLLMSPTL EDQERHLLVK VIDRIL YKL DDLVRPYVHK ILVVIEPLLI DEDYYARVEG REIISNLAKA AGLATMISTM RPDIDNMDEY VRNTTARAFA VVASALG IP SLLPFLKAVC KSKKSWQARH TGIKIVQQIA ILMGCAILPH LRSLVEIIEH GLVDEQQKVR TISALAIAAL AEAATPYG I ESFDSVLKPL WKGIRQHRGK GLAAFLKAIG YLIPLMDAEY ANYYTREVML ILIREFQSPD EEMKKIVLKV VKQCCGTDG VEANYIKTEI LPPFFKHFWQ HRMALDRRNY RQLVDTTVEL ANKVGAAEII SRIVDDLKDE AEQYRKMVME TIEKIMGNLG AADIDHKLE EQLIDGILYA FQEQTTEDSV MLNGFGTVVN ALGKRVKPYL PQICGTVLWR LNNKSAKVRQ QAADLISRTA V VMKTCQEE KLMGHLGVVL YEYLGEEYPE VLGSILGALK AIVNVIGMHK MTPPIKDLLP RLTPILKNRH EKVQENCIDL VG RIADRGA EYVSAREWMR ICFELLELLK AHKKAIRRAT VNTFGYIAKA IGPHDVLATL LNNLKVQERQ NRVCTTVAIA IVA ETCSPF TVLPALMNEY RVPELNVQNG VLKSLSFLFE YIGEMGKDYI YAVTPLLEDA LMDRDLVHRQ TASAVVQHMS LGVY GFGCE DSLNHLLNYV WPNVFETSPH VIQAVMGALE GLRVAIGPCR MLQYCLQGLF HPARKVRDVY WKIYNSIYIG SQDAL IAHY PRIYNDDKNT YIRYELDYIL

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Macromolecule #51: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 51 / Number of copies: 6 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #52: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 52 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #53: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 53 / Number of copies: 1 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

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Macromolecule #54: INOSITOL HEXAKISPHOSPHATE

MacromoleculeName: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 54 / Number of copies: 1 / Formula: IHP
Molecular weightTheoretical: 660.035 Da
Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1.4 mg/mL
BufferpH: 7.9
Component:
ConcentrationName
20.0 mMHepes KOH
50.0 mMKCl
1.0 mMDTT
0.2 mMEDTA
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 6.0 nm / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK III

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Frames/image: 1-40 / Average exposure time: 6.0 sec. / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 1130953
CTF correctionSoftware - Name: Gctf
Startup modelType of model: EMDB MAP
EMDB ID:

6581

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.28 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 86146
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
Final 3D classificationNumber classes: 6 / Software - Name: RELION (ver. 3.0)
FSC plot (resolution estimation)

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